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The map label for this gene is guaA [H]

Identifier: 161523334

GI number: 161523334

Start: 174897

End: 175499

Strand: Reverse

Name: guaA [H]

Synonym: Bmul_0154

Alternate gene names: 161523334

Gene position: 175499-174897 (Counterclockwise)

Preceding gene: 161523337

Following gene: 161523331

Centisome position: 5.09

GC content: 65.17

Gene sequence:

>603_bases
ATGTCGCAGCGATGCAACTGGGTCAAAACCGAAGCGGACGCTCACTATCACGACACCGAGTGGGGTGTGCCGTCGCACGA
CGATCGTCATCTGTTCGAGATGCTGATCCTCGAAGGCGCGCAGGCCGGGCTGTCGTGGTCGACGATCCTGAACAAGCGCG
CCGGTTACCGCGACGCATTCGCCGATTTCGACGTCGACGCCGTCGCGCGCTTCACGCCGAAGCGCATCGAGAAGCTGCTG
GAAAATCCGGGCATCGTGCGCAACCGCGCGAAGGTGGAAGCCGCGGTCGCCAATGCGCGCGCGGTGCAGCGCATCCGTGA
GGAGCACGGTTCGCTCGCGCACTTCCTCTGGTCGTTCGTCGATCACACGCCGATCCAGAACGCGTGGCAGTCGTATCGAG
ACGCACCGGCGTCGACGGAGCGCTCCGATGCGCTCAGCAAGGCGCTGAAGGCGTACGGCTGCAAGTTCGTCGGTTCGACG
ATCTGCTACGCGCTGATGCAGGCAACCGGAATGGTCAACGATCATGAAGTCGGCTGCCCGTGCCATGCGCGATGCGCGGC
GCTCGGCGGCAAACAGCCGGCGCGCGCGCGCAAAGCGGGCTGA

Upstream 100 bases:

>100_bases
ATCGGCGGCGCACGCCCATCTGCCGTACGGCCAGCTATCCCGCGCCGGGGCCTTCCGCTATGCTGGCGCGATGGAATCGA
TACGGGAAGCGAGGCAGACG

Downstream 100 bases:

>100_bases
CGAATCGGCGGCGCGCGGGCAGCCGCCGTTCGGCGCTCAGCGCGCGGGCCGCGACGAAGCGGGTACGGCTGTCCGCGACG
CGCCCCGCGCCGCCCGCAGG

Product: DNA-3-methyladenine glycosylase I

Products: NA

Alternate protein names: GMP synthetase; Glutamine amidotransferase [H]

Number of amino acids: Translated: 200; Mature: 199

Protein sequence:

>200_residues
MSQRCNWVKTEADAHYHDTEWGVPSHDDRHLFEMLILEGAQAGLSWSTILNKRAGYRDAFADFDVDAVARFTPKRIEKLL
ENPGIVRNRAKVEAAVANARAVQRIREEHGSLAHFLWSFVDHTPIQNAWQSYRDAPASTERSDALSKALKAYGCKFVGST
ICYALMQATGMVNDHEVGCPCHARCAALGGKQPARARKAG

Sequences:

>Translated_200_residues
MSQRCNWVKTEADAHYHDTEWGVPSHDDRHLFEMLILEGAQAGLSWSTILNKRAGYRDAFADFDVDAVARFTPKRIEKLL
ENPGIVRNRAKVEAAVANARAVQRIREEHGSLAHFLWSFVDHTPIQNAWQSYRDAPASTERSDALSKALKAYGCKFVGST
ICYALMQATGMVNDHEVGCPCHARCAALGGKQPARARKAG
>Mature_199_residues
SQRCNWVKTEADAHYHDTEWGVPSHDDRHLFEMLILEGAQAGLSWSTILNKRAGYRDAFADFDVDAVARFTPKRIEKLLE
NPGIVRNRAKVEAAVANARAVQRIREEHGSLAHFLWSFVDHTPIQNAWQSYRDAPASTERSDALSKALKAYGCKFVGSTI
CYALMQATGMVNDHEVGCPCHARCAALGGKQPARARKAG

Specific function: Catalyzes the synthesis of GMP from XMP [H]

COG id: COG2818

COG function: function code L; 3-methyladenine DNA glycosylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 N-acetyltransferase domains [H]

Homologues:

Organism=Escherichia coli, GI1789971, Length=181, Percent_Identity=49.171270718232, Blast_Score=188, Evalue=2e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000182
- InterPro:   IPR016181
- InterPro:   IPR005019
- InterPro:   IPR001962
- InterPro:   IPR001317
- InterPro:   IPR011257
- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR001674
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00583 Acetyltransf_1; PF03352 Adenine_glyco; PF00733 Asn_synthase; PF00117 GATase; PF00958 GMP_synt_C [H]

EC number: =6.3.5.2 [H]

Molecular weight: Translated: 22224; Mature: 22093

Theoretical pI: Translated: 8.38; Mature: 8.38

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
3.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQRCNWVKTEADAHYHDTEWGVPSHDDRHLFEMLILEGAQAGLSWSTILNKRAGYRDAF
CCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHCCCHHHH
ADFDVDAVARFTPKRIEKLLENPGIVRNRAKVEAAVANARAVQRIREEHGSLAHFLWSFV
HCCCHHHHHHCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DHTPIQNAWQSYRDAPASTERSDALSKALKAYGCKFVGSTICYALMQATGMVNDHEVGCP
CCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCC
CHARCAALGGKQPARARKAG
HHHHHHHHCCCCCCHHCCCC
>Mature Secondary Structure 
SQRCNWVKTEADAHYHDTEWGVPSHDDRHLFEMLILEGAQAGLSWSTILNKRAGYRDAF
CCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHCCCHHHH
ADFDVDAVARFTPKRIEKLLENPGIVRNRAKVEAAVANARAVQRIREEHGSLAHFLWSFV
HCCCHHHHHHCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DHTPIQNAWQSYRDAPASTERSDALSKALKAYGCKFVGSTICYALMQATGMVNDHEVGCP
CCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCC
CHARCAALGGKQPARARKAG
HHHHHHHHCCCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12810954 [H]