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The map label for this gene is gcvH

Identifier: 161523322

GI number: 161523322

Start: 159516

End: 159896

Strand: Reverse

Name: gcvH

Synonym: Bmul_0142

Alternate gene names: 161523322

Gene position: 159896-159516 (Counterclockwise)

Preceding gene: 161523323

Following gene: 161523321

Centisome position: 4.64

GC content: 63.78

Gene sequence:

>381_bases
ATGAGCAACGTCCCGGCCGATCTGAAGTACACCGAGGAACACGAGTGGATCCGTACCGAAGCAGACGGCACGCTGACGGT
CGGCATCACCGATCACGCGCAGAGCACGCTCGGCGACATCGTCTTCCTCGAACTGCCGGAAGTCGGCAAGCAGGTGACGG
CCGGCGACGCGGTGGGCGTCGTCGAGTCGGTGAAGGCCGCGTCCGACATCTATTCGCCGGTGTCGGGCGAAATCGTCGAG
ATCAACTCGGCGATCGTCGACACGCCGGACTCGGTCAACAGCGACGCATACGCGAGCTGGCTGTTCAAGATCAAGCTCGC
CGACGGCGCATCGACCGACAAGCTGATCGACGCGGCCGCCTACGGCAAGCTGATCGACTGA

Upstream 100 bases:

>100_bases
TCCCGGTTCGCCGGGATATGGCGCCCGGCAAAACAACCGCATCGGCGCGCCCGCCCGGCGCGCCGGCACAACGAATCACA
CTTTTATCCAGGAGCATCCG

Downstream 100 bases:

>100_bases
TTTTTCCGACCCGAAACGCGCGGCGCCGGCAGCCATCCGGTGCCGCGCAAGCCGGAGGCGGCCGCCGCGCCGCTTCCGGT
CGACCGCCCAGCATGGGGCC

Product: glycine cleavage system protein H

Products: Proton; NADH; NH3; CO2; 5,10-methylene-THF [C]

Alternate protein names: NA

Number of amino acids: Translated: 126; Mature: 125

Protein sequence:

>126_residues
MSNVPADLKYTEEHEWIRTEADGTLTVGITDHAQSTLGDIVFLELPEVGKQVTAGDAVGVVESVKAASDIYSPVSGEIVE
INSAIVDTPDSVNSDAYASWLFKIKLADGASTDKLIDAAAYGKLID

Sequences:

>Translated_126_residues
MSNVPADLKYTEEHEWIRTEADGTLTVGITDHAQSTLGDIVFLELPEVGKQVTAGDAVGVVESVKAASDIYSPVSGEIVE
INSAIVDTPDSVNSDAYASWLFKIKLADGASTDKLIDAAAYGKLID
>Mature_125_residues
SNVPADLKYTEEHEWIRTEADGTLTVGITDHAQSTLGDIVFLELPEVGKQVTAGDAVGVVESVKAASDIYSPVSGEIVEI
NSAIVDTPDSVNSDAYASWLFKIKLADGASTDKLIDAAAYGKLID

Specific function: The glycine cleavage system catalyzes the degradation of glycine. The H protein shuttles the methylamine group of glycine from the P protein to the T protein

COG id: COG0509

COG function: function code E; Glycine cleavage system H protein (lipoate-binding)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain

Homologues:

Organism=Homo sapiens, GI49574537, Length=119, Percent_Identity=47.8991596638655, Blast_Score=118, Evalue=1e-27,
Organism=Homo sapiens, GI89057342, Length=119, Percent_Identity=47.0588235294118, Blast_Score=115, Evalue=1e-26,
Organism=Escherichia coli, GI1789271, Length=127, Percent_Identity=62.992125984252, Blast_Score=166, Evalue=4e-43,
Organism=Caenorhabditis elegans, GI17551294, Length=116, Percent_Identity=45.6896551724138, Blast_Score=105, Evalue=5e-24,
Organism=Caenorhabditis elegans, GI17507493, Length=116, Percent_Identity=43.1034482758621, Blast_Score=104, Evalue=9e-24,
Organism=Saccharomyces cerevisiae, GI6319272, Length=115, Percent_Identity=42.6086956521739, Blast_Score=105, Evalue=1e-24,
Organism=Drosophila melanogaster, GI17865652, Length=120, Percent_Identity=41.6666666666667, Blast_Score=107, Evalue=2e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GCSH_BURM1 (A9ACU4)

Other databases:

- EMBL:   CP000868
- EMBL:   AP009385
- RefSeq:   YP_001578334.1
- RefSeq:   YP_001947533.1
- ProteinModelPortal:   A9ACU4
- SMR:   A9ACU4
- GeneID:   5765520
- GeneID:   6359385
- GenomeReviews:   AP009385_GR
- GenomeReviews:   CP000868_GR
- KEGG:   bmj:BMULJ_03123
- KEGG:   bmu:Bmul_0142
- HOGENOM:   HBG693789
- OMA:   TSDHEWL
- ProtClustDB:   PRK01202
- GO:   GO:0005739
- HAMAP:   MF_00272
- InterPro:   IPR003016
- InterPro:   IPR002930
- InterPro:   IPR017453
- InterPro:   IPR011053
- PANTHER:   PTHR11715
- TIGRFAMs:   TIGR00527

Pfam domain/function: PF01597 GCV_H; SSF51230 Hybrid_motif

EC number: NA

Molecular weight: Translated: 13328; Mature: 13197

Theoretical pI: Translated: 3.93; Mature: 3.93

Prosite motif: PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
0.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
0.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNVPADLKYTEEHEWIRTEADGTLTVGITDHAQSTLGDIVFLELPEVGKQVTAGDAVGV
CCCCCCCCEECCCCCEEEECCCCEEEEEECCCCHHHHCCEEEEECCCCCCEECCCCHHHH
VESVKAASDIYSPVSGEIVEINSAIVDTPDSVNSDAYASWLFKIKLADGASTDKLIDAAA
HHHHHHHHHHCCCCCCCEEEECCEEECCCCCCCCCCEEEEEEEEEECCCCCCHHHHHHHH
YGKLID
HCCCCC
>Mature Secondary Structure 
SNVPADLKYTEEHEWIRTEADGTLTVGITDHAQSTLGDIVFLELPEVGKQVTAGDAVGV
CCCCCCCEECCCCCEEEECCCCEEEEEECCCCHHHHCCEEEEECCCCCCEECCCCHHHH
VESVKAASDIYSPVSGEIVEINSAIVDTPDSVNSDAYASWLFKIKLADGASTDKLIDAAA
HHHHHHHHHHCCCCCCCEEEECCEEECCCCCCCCCCEEEEEEEEEECCCCCCHHHHHHHH
YGKLID
HCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Lipoyl Cofactor. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NAD; L-glycine; THF [C]

Specific reaction: NAD + L-glycine + THF = Proton + NADH + NH3 + CO2 + 5,10-methylene-THF [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA