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The map label for this gene is argT [H]

Identifier: 161523299

GI number: 161523299

Start: 130522

End: 131310

Strand: Direct

Name: argT [H]

Synonym: Bmul_0119

Alternate gene names: 161523299

Gene position: 130522-131310 (Clockwise)

Preceding gene: 161523298

Following gene: 161523300

Centisome position: 3.78

GC content: 66.67

Gene sequence:

>789_bases
ATGAAACCCACGCACTGCCTGAAACTGGCGATCGCGCTCGCATGCGCGCTCGGTTCCGCCGCGGCCGTCGCCGACGCGCA
GACGCTGCGCTTCGGCCTCGAGGCGCAATATCCGCCGTTCGAATCGAAAGCGCCGAACGGCGAACTGCAGGGCTTCGACA
TCGACGTCGGCAATGCGGTGTGCGAAACCGCGAAGCTGACGTGCAAATGGGTCGAGACCTCGTTCGACGGGCTGATTCCG
GCGCTGAAGGGTCGCAAGTTCGACGCGATCAACTCGGCGATGAACGCGACCGAGCAGCGCCGTCAGGCGATCGACTTCAC
GACGATCATCTACCGCGTGCCGACGCAGCTGATCGCGCGCACCGGCAGCGGGCTGCTGCCGACGCCCGAATCGCTGAAGG
GCAAGCGCGTCGGCGTGTTGCAGGCGTCGATTCAGGAAACCTATGCGAAGGCGCACTGGGAGCCGGCCGGCGTCGCGATC
GTCGCCTATCAGGATCAGAACCAGGCCTATGCCGATCTCGTCGCGGGCCGCCTCGATGCGACGCTCGTGCTTGCGCCGGC
CGGCCAGCGCGGCTTCCTGTCGCGTCCGGACGGCAAGGGCTTCTCGTTCGTCGGCCAGCCGGTGCGCGACGACCGGATCC
TCGGCAGCGGCATCGCGTTCGGCTTGCGCAAGGGCGACGACGCGCTGAAGGCGAAGCTCGACGCGGCGATCGACAAGCTG
AAGGCGGACGGCACCGTGAAGTCGCTCGGCCGCAAGTATTTCGGCGACATCGACATCTCGGCGAAGTAA

Upstream 100 bases:

>100_bases
TTTCTCGAAGGACGACGCGACGCTGATCGAAGGCGCGCGGCGCTTGCGTTCGCTGTAGGCGCGGCGCCCCCGCCGCTGCA
ATCGACAACAGGAGATCACG

Downstream 100 bases:

>100_bases
GCGGGACCGCACGATGCCGATGCCCGATACGGCCGCCGCGTGCGGCACACCGCGCGAGGCGCGGCTGTCGGATGCCGCGC
TCGAAGCGGCGCTCGTCGCC

Product: extracellular solute-binding protein

Products: ADP; phosphate; L-ornithine [Cytoplasm]; ADP; L-arginine [Cytoplasm]; L-lysine [Cytoplasm] [C]

Alternate protein names: LAO-binding protein [H]

Number of amino acids: Translated: 262; Mature: 262

Protein sequence:

>262_residues
MKPTHCLKLAIALACALGSAAAVADAQTLRFGLEAQYPPFESKAPNGELQGFDIDVGNAVCETAKLTCKWVETSFDGLIP
ALKGRKFDAINSAMNATEQRRQAIDFTTIIYRVPTQLIARTGSGLLPTPESLKGKRVGVLQASIQETYAKAHWEPAGVAI
VAYQDQNQAYADLVAGRLDATLVLAPAGQRGFLSRPDGKGFSFVGQPVRDDRILGSGIAFGLRKGDDALKAKLDAAIDKL
KADGTVKSLGRKYFGDIDISAK

Sequences:

>Translated_262_residues
MKPTHCLKLAIALACALGSAAAVADAQTLRFGLEAQYPPFESKAPNGELQGFDIDVGNAVCETAKLTCKWVETSFDGLIP
ALKGRKFDAINSAMNATEQRRQAIDFTTIIYRVPTQLIARTGSGLLPTPESLKGKRVGVLQASIQETYAKAHWEPAGVAI
VAYQDQNQAYADLVAGRLDATLVLAPAGQRGFLSRPDGKGFSFVGQPVRDDRILGSGIAFGLRKGDDALKAKLDAAIDKL
KADGTVKSLGRKYFGDIDISAK
>Mature_262_residues
MKPTHCLKLAIALACALGSAAAVADAQTLRFGLEAQYPPFESKAPNGELQGFDIDVGNAVCETAKLTCKWVETSFDGLIP
ALKGRKFDAINSAMNATEQRRQAIDFTTIIYRVPTQLIARTGSGLLPTPESLKGKRVGVLQASIQETYAKAHWEPAGVAI
VAYQDQNQAYADLVAGRLDATLVLAPAGQRGFLSRPDGKGFSFVGQPVRDDRILGSGIAFGLRKGDDALKAKLDAAIDKL
KADGTVKSLGRKYFGDIDISAK

Specific function: This periplasmic binding protein is involved in an arginine transport system. ArgT and histidine-binding protein J (hisJ) interact with a common membrane-bound receptor, hisP [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1788649, Length=253, Percent_Identity=45.0592885375494, Blast_Score=224, Evalue=3e-60,
Organism=Escherichia coli, GI1788648, Length=233, Percent_Identity=45.9227467811159, Blast_Score=222, Evalue=2e-59,
Organism=Escherichia coli, GI1787088, Length=250, Percent_Identity=33.6, Blast_Score=136, Evalue=2e-33,
Organism=Escherichia coli, GI1787085, Length=249, Percent_Identity=33.7349397590361, Blast_Score=135, Evalue=2e-33,
Organism=Escherichia coli, GI1788228, Length=256, Percent_Identity=28.125, Blast_Score=102, Evalue=3e-23,
Organism=Escherichia coli, GI1787031, Length=247, Percent_Identity=28.7449392712551, Blast_Score=86, Evalue=2e-18,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005768
- InterPro:   IPR001638
- InterPro:   IPR018313 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 27925; Mature: 27925

Theoretical pI: Translated: 9.13; Mature: 9.13

Prosite motif: PS01039 SBP_BACTERIAL_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPTHCLKLAIALACALGSAAAVADAQTLRFGLEAQYPPFESKAPNGELQGFDIDVGNAV
CCCHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEECCHHH
CETAKLTCKWVETSFDGLIPALKGRKFDAINSAMNATEQRRQAIDFTTIIYRVPTQLIAR
HHHHHEEEEEECCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TGSGLLPTPESLKGKRVGVLQASIQETYAKAHWEPAGVAIVAYQDQNQAYADLVAGRLDA
CCCCCCCCCHHCCCCEEEHHHHHHHHHHHHCCCCCCCEEEEEEECCCHHHHHHHHHCCCE
TLVLAPAGQRGFLSRPDGKGFSFVGQPVRDDRILGSGIAFGLRKGDDALKAKLDAAIDKL
EEEEECCCCCCCCCCCCCCCCCCCCCCCCCCEEECCCCEEECCCCCHHHHHHHHHHHHHH
KADGTVKSLGRKYFGDIDISAK
CCCCHHHHHHHHHCCCEEECCC
>Mature Secondary Structure
MKPTHCLKLAIALACALGSAAAVADAQTLRFGLEAQYPPFESKAPNGELQGFDIDVGNAV
CCCHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEECCHHH
CETAKLTCKWVETSFDGLIPALKGRKFDAINSAMNATEQRRQAIDFTTIIYRVPTQLIAR
HHHHHEEEEEECCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TGSGLLPTPESLKGKRVGVLQASIQETYAKAHWEPAGVAIVAYQDQNQAYADLVAGRLDA
CCCCCCCCCHHCCCCEEEHHHHHHHHHHHHCCCCCCCEEEEEEECCCHHHHHHHHHCCCE
TLVLAPAGQRGFLSRPDGKGFSFVGQPVRDDRILGSGIAFGLRKGDDALKAKLDAAIDKL
EEEEECCCCCCCCCCCCCCCCCCCCCCCCCCEEECCCCEEECCCCCHHHHHHHHHHHHHH
KADGTVKSLGRKYFGDIDISAK
CCCCHHHHHHHHHCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-ornithine [Periplasm]; H2O; ATP; L-arginine [Periplasm]; L-lysine [Periplasm] [C]

Specific reaction: ATP + L-ornithine [Periplasm] + H2O = ADP + phosphate + L-ornithine [Cytoplasm] ATP + L-arginine [Periplasm] + H2O = ADP + phosphate + L-arginine [Cytoplasm] ATP + L-lysine [Periplasm] + H2O = ADP + phosphate + L-lysine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503; 3040734; 9298646 [H]