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The map label for this gene is gidB [H]

Identifier: 161523274

GI number: 161523274

Start: 101708

End: 102394

Strand: Direct

Name: gidB [H]

Synonym: Bmul_0094

Alternate gene names: 161523274

Gene position: 101708-102394 (Clockwise)

Preceding gene: 161523273

Following gene: 161523275

Centisome position: 2.95

GC content: 64.92

Gene sequence:

>687_bases
ATGACGGCGCGTCGCGCGCCGGCGGTTAATCGGGACGTACTTGAACGGATGCTCGTCGACGGCGCGGCGTCGCTCGACGT
CGCGCTATCCGATACGCAACGCAACCGGCTGCTCGACTACGTCGCGCTGCTGGGCAAGTGGAACGCGGTCTACAACCTGA
CCGCGATTCGCGATCCGCAGCAGATGCTGATCCAGCACATCCTCGATTCGCTGTCGATCGTGCCGCATCTGCGCGGCCGC
GCGTCGGCACGCGTGCTCGACGTCGGCTCGGGCGGCGGGTTGCCGGGCCTCGTGCTCGCGATCGTCGAGCCGGACTGGCA
GGTAACGCTGAACGACATCGTGCAGAAGAAGTCCGCGTTCCAGACGCAAACGCGTGCGGAACTGAAGCTCACGAATCTGT
CGGTGGTCACGGGGCGCGTCGAAACGCTGCGGCCTGGCGTCGAGGTGCCGGAAAAATTCGACATCATCGTGTCGCGCGCC
TTCGCGGACCTGTCCGACTTCGTTAAACTTGCTCGGCATCTCGTTGCGCCGGGCGGTTCGATCTGGGCGATGAAGGGCGT
GCATCCGGACGACGAGATTGCGCGGTTGCCCGAAGGCAGCCGCGTGAAGCAGACGATGCGTCTGGCGGTTCCGATGCTCG
ATGCCGAACGGCATCTGATCGAGGTGGCCGTCGACGAGGCGATTTGA

Upstream 100 bases:

>100_bases
CGCTGCTGATGGTGCACTTGAAGCGGCGCGGGCTGGGCCGCCGTAACGGCGCGGCTGCACCGGCTGCCGAGCAGGGGGAC
GGCGCCGTCCCGACGCAACA

Downstream 100 bases:

>100_bases
CGGCGCGCGAACGGCGCGCCGAATGTTTGAAGGTAAGAGGAACACACCAACGATGGCAAAGATCTTCTGCGTTGCGAACC
AGAAGGGGGGCGTCGGCAAG

Product: 16S rRNA methyltransferase GidB

Products: NA

Alternate protein names: 16S rRNA 7-methylguanosine methyltransferase; 16S rRNA m7G methyltransferase [H]

Number of amino acids: Translated: 228; Mature: 227

Protein sequence:

>228_residues
MTARRAPAVNRDVLERMLVDGAASLDVALSDTQRNRLLDYVALLGKWNAVYNLTAIRDPQQMLIQHILDSLSIVPHLRGR
ASARVLDVGSGGGLPGLVLAIVEPDWQVTLNDIVQKKSAFQTQTRAELKLTNLSVVTGRVETLRPGVEVPEKFDIIVSRA
FADLSDFVKLARHLVAPGGSIWAMKGVHPDDEIARLPEGSRVKQTMRLAVPMLDAERHLIEVAVDEAI

Sequences:

>Translated_228_residues
MTARRAPAVNRDVLERMLVDGAASLDVALSDTQRNRLLDYVALLGKWNAVYNLTAIRDPQQMLIQHILDSLSIVPHLRGR
ASARVLDVGSGGGLPGLVLAIVEPDWQVTLNDIVQKKSAFQTQTRAELKLTNLSVVTGRVETLRPGVEVPEKFDIIVSRA
FADLSDFVKLARHLVAPGGSIWAMKGVHPDDEIARLPEGSRVKQTMRLAVPMLDAERHLIEVAVDEAI
>Mature_227_residues
TARRAPAVNRDVLERMLVDGAASLDVALSDTQRNRLLDYVALLGKWNAVYNLTAIRDPQQMLIQHILDSLSIVPHLRGRA
SARVLDVGSGGGLPGLVLAIVEPDWQVTLNDIVQKKSAFQTQTRAELKLTNLSVVTGRVETLRPGVEVPEKFDIIVSRAF
ADLSDFVKLARHLVAPGGSIWAMKGVHPDDEIARLPEGSRVKQTMRLAVPMLDAERHLIEVAVDEAI

Specific function: Specifically methylates the N7 position of guanosine in position 527 of 16S rRNA [H]

COG id: COG0357

COG function: function code M; Predicted S-adenosylmethionine-dependent methyltransferase involved in bacterial cell division

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RNA methyltransferase rsmG family [H]

Homologues:

Organism=Escherichia coli, GI1790179, Length=202, Percent_Identity=45.5445544554455, Blast_Score=181, Evalue=5e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003682 [H]

Pfam domain/function: PF02527 GidB [H]

EC number: =2.1.1.170 [H]

Molecular weight: Translated: 25018; Mature: 24887

Theoretical pI: Translated: 7.10; Mature: 7.10

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTARRAPAVNRDVLERMLVDGAASLDVALSDTQRNRLLDYVALLGKWNAVYNLTAIRDPQ
CCCCCCCCCCHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHHHCCCCEEEEEECCCHH
QMLIQHILDSLSIVPHLRGRASARVLDVGSGGGLPGLVLAIVEPDWQVTLNDIVQKKSAF
HHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCEEEEEECCCCEEEHHHHHHHHHHH
QTQTRAELKLTNLSVVTGRVETLRPGVEVPEKFDIIVSRAFADLSDFVKLARHLVAPGGS
HHHHHHHEEEEEEEEEECCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
IWAMKGVHPDDEIARLPEGSRVKQTMRLAVPMLDAERHLIEVAVDEAI
EEEECCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TARRAPAVNRDVLERMLVDGAASLDVALSDTQRNRLLDYVALLGKWNAVYNLTAIRDPQ
CCCCCCCCCHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHHHCCCCEEEEEECCCHH
QMLIQHILDSLSIVPHLRGRASARVLDVGSGGGLPGLVLAIVEPDWQVTLNDIVQKKSAF
HHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCEEEEEECCCCEEEHHHHHHHHHHH
QTQTRAELKLTNLSVVTGRVETLRPGVEVPEKFDIIVSRAFADLSDFVKLARHLVAPGGS
HHHHHHHEEEEEEEEEECCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
IWAMKGVHPDDEIARLPEGSRVKQTMRLAVPMLDAERHLIEVAVDEAI
EEEECCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA