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The map label for this gene is mnmG [H]

Identifier: 161523273

GI number: 161523273

Start: 99741

End: 101711

Strand: Direct

Name: mnmG [H]

Synonym: Bmul_0093

Alternate gene names: 161523273

Gene position: 99741-101711 (Clockwise)

Preceding gene: 161523272

Following gene: 161523274

Centisome position: 2.89

GC content: 65.96

Gene sequence:

>1971_bases
ATGCTTTATCCCACAGAATTTGACGTCATCGTCGTCGGCGGCGGTCATGCCGGCACGGAAGCCGCGTTGGCGTCCGCCCG
AATGGGCGCGAAGACGCTGCTGCTGACCCACAACATCGAAACGCTCGGGCAGATGAGCTGCAATCCGTCGATCGGCGGCA
TCGGCAAGGGCCATCTGGTCAAGGAAGTCGATGCGCTGGGCGGCGCGATGGCGGCCGCCACCGACGAAGCGGGGATCCAG
TTCCGGATCCTGAATTCGTCGAAAGGCCCGGCCGTACGCGCGACACGCGCCCAGGCGGACCGCATCCTGTACAAGGCGGC
GATCCGTCGCCGCCTCGAGAATCAGCCGAATCTGTGGCTGTTCCAGCAGGCCGTCGACGACCTGATGGTCGAGGGCGATT
GCGTGGTCGGCGCCGTCACGCAAATCGGCATCCGGTTCCGCGCGCGCGCGGTCGTGCTGACGGCCGGTACATTCCTGGAC
GGCAAGATCCACGTCGGCCTGAACAACTATACGGGCGGTCGCGCGGGCGACCCGGCCGCCGTGTCGCTGTCTGCGCGCTT
GAAGGAGCTGAAGCTGCCGCAAGGCCGGCTGAAGACCGGAACGCCGCCGCGTATCGACGGGCGCACGATCGATTTCTCGA
AGCTCGAAGAGCAGCCGGGCGATCTCGATCCGATTCCGGTGTTCTCGTTCCTCGGCCGTGCCGAGCAGCATCCGCAGCAG
CTGCCGTGCTGGGTCACGCACACGAACGAGCGCACGCACGACATCATTCGCGGCGGCCTCGACCGTTCGCCGATGTACAC
GGGCGTGATCGAAGGCGTGGGGCCGCGCTACTGCCCGTCGATCGAGGACAAGATTCATCGATTCGCGTCGAAGGAATCGC
ACCAGATCTTCCTCGAGCCGGAAGGGCTGACGACCAACGAGTTCTACCCGAACGGGATCTCGACGAGCCTGCCGTTCGAC
GTGCAGCTCGAGCTCGTGCATTCGATGCGCGGCCTCGAACAGGCGCATATCCTGCGGCCCGGCTATGCGATCGAGTATGA
CTACTTCGACCCGCGCGCGCTGAAGGCGTCGCTGGAGACGAAGGCGATCAACGGATTGTTCTTTGCGGGCCAGATCAACG
GGACGACGGGCTACGAAGAGGCCGCGGCGCAGGGCCTGCTCGCAGGCGTCAATGCGGGCCGCTACGTGCAGGAAAAGGAC
GCATGGTGCCCGCGTCGCGATCAGGCGTACCTGGGCGTGCTGGTCGACGATCTCGTCACGCGCGGCGTCGCCGAGCCGTA
CCGGATGTTCACGAGCCGGGCCGAATACCGCCTGAGCCTTCGCGAAGACAACGCGGACATGCGTCTGACCGAGATCGGCC
GCGAGCTGGGCCTCGTCGACGACGTGCGTTGGGACGCGTTCTGCCGGAAGCGCGACGCTGTTTCACGTGAAACCGAACGA
CTGAAGACGACCTGGGTGACGCCGAAGACGCTGCCGCCGGAAGAGGCGACCGCATTGCTCGGCAAACCGATCGACCACGA
ATACAGCCTGGCGGAGCTGCTGCGGCGTCCGGGCGTCGACTACGACGGCGTCTGCGGGCTGAAGGGCGGCGAGTGCGGGC
CGGCTGAGCCGCTGGCGGACGATCCGGTGCTGCTCGCGCAGATCAAGGAGCAGGTCGAGATCGGCATCAAGTATCAGGGC
TATATCGAACGTCAGGCGACCGAGATCGAACGCAACGACGCGAACGAGAACACGCGTCTGCCGGACGGGATCGACTATCG
CGAAGTACGCGGCTTGTCGTTCGAGGTCAGTCAGAAGCTCAATCAGTTCCGGCCGGAGACGATCGGGCAGGCGTCGCGCA
TCTCCGGCGTCACGCCGGCGGCCATTTCGCTGCTGATGGTGCACTTGAAGCGGCGCGGGCTGGGCCGCCGTAACGGCGCG
GCTGCACCGGCTGCCGAGCAGGGGGACGGCGCCGTCCCGACGCAACAATGA

Upstream 100 bases:

>100_bases
TACCATTCGGACGACCTTCGGGCGAAAAGCGAACCCGCTATAATTCGCCCCATACATTTCTCAGATAGGTTCGTGCGCGG
TCCTGCGTACGGAATCCACC

Downstream 100 bases:

>100_bases
CGGCGCGTCGCGCGCCGGCGGTTAATCGGGACGTACTTGAACGGATGCTCGTCGACGGCGCGGCGTCGCTCGACGTCGCG
CTATCCGATACGCAACGCAA

Product: tRNA uridine 5-carboxymethylaminomethyl modification protein GidA

Products: NA

Alternate protein names: Glucose-inhibited division protein A [H]

Number of amino acids: Translated: 656; Mature: 656

Protein sequence:

>656_residues
MLYPTEFDVIVVGGGHAGTEAALASARMGAKTLLLTHNIETLGQMSCNPSIGGIGKGHLVKEVDALGGAMAAATDEAGIQ
FRILNSSKGPAVRATRAQADRILYKAAIRRRLENQPNLWLFQQAVDDLMVEGDCVVGAVTQIGIRFRARAVVLTAGTFLD
GKIHVGLNNYTGGRAGDPAAVSLSARLKELKLPQGRLKTGTPPRIDGRTIDFSKLEEQPGDLDPIPVFSFLGRAEQHPQQ
LPCWVTHTNERTHDIIRGGLDRSPMYTGVIEGVGPRYCPSIEDKIHRFASKESHQIFLEPEGLTTNEFYPNGISTSLPFD
VQLELVHSMRGLEQAHILRPGYAIEYDYFDPRALKASLETKAINGLFFAGQINGTTGYEEAAAQGLLAGVNAGRYVQEKD
AWCPRRDQAYLGVLVDDLVTRGVAEPYRMFTSRAEYRLSLREDNADMRLTEIGRELGLVDDVRWDAFCRKRDAVSRETER
LKTTWVTPKTLPPEEATALLGKPIDHEYSLAELLRRPGVDYDGVCGLKGGECGPAEPLADDPVLLAQIKEQVEIGIKYQG
YIERQATEIERNDANENTRLPDGIDYREVRGLSFEVSQKLNQFRPETIGQASRISGVTPAAISLLMVHLKRRGLGRRNGA
AAPAAEQGDGAVPTQQ

Sequences:

>Translated_656_residues
MLYPTEFDVIVVGGGHAGTEAALASARMGAKTLLLTHNIETLGQMSCNPSIGGIGKGHLVKEVDALGGAMAAATDEAGIQ
FRILNSSKGPAVRATRAQADRILYKAAIRRRLENQPNLWLFQQAVDDLMVEGDCVVGAVTQIGIRFRARAVVLTAGTFLD
GKIHVGLNNYTGGRAGDPAAVSLSARLKELKLPQGRLKTGTPPRIDGRTIDFSKLEEQPGDLDPIPVFSFLGRAEQHPQQ
LPCWVTHTNERTHDIIRGGLDRSPMYTGVIEGVGPRYCPSIEDKIHRFASKESHQIFLEPEGLTTNEFYPNGISTSLPFD
VQLELVHSMRGLEQAHILRPGYAIEYDYFDPRALKASLETKAINGLFFAGQINGTTGYEEAAAQGLLAGVNAGRYVQEKD
AWCPRRDQAYLGVLVDDLVTRGVAEPYRMFTSRAEYRLSLREDNADMRLTEIGRELGLVDDVRWDAFCRKRDAVSRETER
LKTTWVTPKTLPPEEATALLGKPIDHEYSLAELLRRPGVDYDGVCGLKGGECGPAEPLADDPVLLAQIKEQVEIGIKYQG
YIERQATEIERNDANENTRLPDGIDYREVRGLSFEVSQKLNQFRPETIGQASRISGVTPAAISLLMVHLKRRGLGRRNGA
AAPAAEQGDGAVPTQQ
>Mature_656_residues
MLYPTEFDVIVVGGGHAGTEAALASARMGAKTLLLTHNIETLGQMSCNPSIGGIGKGHLVKEVDALGGAMAAATDEAGIQ
FRILNSSKGPAVRATRAQADRILYKAAIRRRLENQPNLWLFQQAVDDLMVEGDCVVGAVTQIGIRFRARAVVLTAGTFLD
GKIHVGLNNYTGGRAGDPAAVSLSARLKELKLPQGRLKTGTPPRIDGRTIDFSKLEEQPGDLDPIPVFSFLGRAEQHPQQ
LPCWVTHTNERTHDIIRGGLDRSPMYTGVIEGVGPRYCPSIEDKIHRFASKESHQIFLEPEGLTTNEFYPNGISTSLPFD
VQLELVHSMRGLEQAHILRPGYAIEYDYFDPRALKASLETKAINGLFFAGQINGTTGYEEAAAQGLLAGVNAGRYVQEKD
AWCPRRDQAYLGVLVDDLVTRGVAEPYRMFTSRAEYRLSLREDNADMRLTEIGRELGLVDDVRWDAFCRKRDAVSRETER
LKTTWVTPKTLPPEEATALLGKPIDHEYSLAELLRRPGVDYDGVCGLKGGECGPAEPLADDPVLLAQIKEQVEIGIKYQG
YIERQATEIERNDANENTRLPDGIDYREVRGLSFEVSQKLNQFRPETIGQASRISGVTPAAISLLMVHLKRRGLGRRNGA
AAPAAEQGDGAVPTQQ

Specific function: NAD-binding protein involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA-cmnm(5)s(2)U34 [H]

COG id: COG0445

COG function: function code D; NAD/FAD-utilizing enzyme apparently involved in cell division

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MnmG family [H]

Homologues:

Organism=Homo sapiens, GI74024895, Length=645, Percent_Identity=44.4961240310078, Blast_Score=487, Evalue=1e-137,
Organism=Homo sapiens, GI19882217, Length=670, Percent_Identity=42.8358208955224, Blast_Score=473, Evalue=1e-133,
Organism=Homo sapiens, GI183227703, Length=685, Percent_Identity=41.8978102189781, Blast_Score=468, Evalue=1e-132,
Organism=Escherichia coli, GI2367273, Length=639, Percent_Identity=69.4835680751174, Blast_Score=903, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17534255, Length=633, Percent_Identity=41.390205371248, Blast_Score=472, Evalue=1e-133,
Organism=Saccharomyces cerevisiae, GI6321202, Length=638, Percent_Identity=45.9247648902821, Blast_Score=511, Evalue=1e-145,
Organism=Drosophila melanogaster, GI24658174, Length=653, Percent_Identity=45.0229709035222, Blast_Score=497, Evalue=1e-140,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004416
- InterPro:   IPR002218
- InterPro:   IPR020595 [H]

Pfam domain/function: PF01134 GIDA [H]

EC number: NA

Molecular weight: Translated: 71886; Mature: 71886

Theoretical pI: Translated: 6.22; Mature: 6.22

Prosite motif: PS01280 GIDA_1 ; PS01281 GIDA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLYPTEFDVIVVGGGHAGTEAALASARMGAKTLLLTHNIETLGQMSCNPSIGGIGKGHLV
CCCCCCCCEEEEECCCCCHHHHHHHHHCCCEEEEEEECHHHHHCCCCCCCCCCCCCCHHH
KEVDALGGAMAAATDEAGIQFRILNSSKGPAVRATRAQADRILYKAAIRRRLENQPNLWL
HHHHHHCCHHHHCCCCCCEEEEEEECCCCCCEEEHHHHHHHHHHHHHHHHHHCCCCCEEE
FQQAVDDLMVEGDCVVGAVTQIGIRFRARAVVLTAGTFLDGKIHVGLNNYTGGRAGDPAA
HHHHHHHHHCCCCEEEEHHHHHCCEEEEEEEEEEECEEECCEEEEEECCCCCCCCCCCCE
VSLSARLKELKLPQGRLKTGTPPRIDGRTIDFSKLEEQPGDLDPIPVFSFLGRAEQHPQQ
EEHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCCCCCHHHHHHCHHHCCCCC
LPCWVTHTNERTHDIIRGGLDRSPMYTGVIEGVGPRYCPSIEDKIHRFASKESHQIFLEP
CCEEEECCCCHHHHHHHCCCCCCCCHHHHHHCCCCCCCCCHHHHHHHHHCCCCCEEEECC
EGLTTNEFYPNGISTSLPFDVQLELVHSMRGLEQAHILRPGYAIEYDYFDPRALKASLET
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCHHHEECCCEEEEECCCCCHHHHHHHHH
KAINGLFFAGQINGTTGYEEAAAQGLLAGVNAGRYVQEKDAWCPRRDQAYLGVLVDDLVT
HHCCCEEEEEEECCCCCHHHHHHHHHHHCCCCCCEECCCCCCCCCCCCHHHHHHHHHHHH
RGVAEPYRMFTSRAEYRLSLREDNADMRLTEIGRELGLVDDVRWDAFCRKRDAVSRETER
HCCCHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHCCHHCCHHHHHHHHHHHHHHHHHH
LKTTWVTPKTLPPEEATALLGKPIDHEYSLAELLRRPGVDYDGVCGLKGGECGPAEPLAD
HEEEECCCCCCCHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCC
DPVLLAQIKEQVEIGIKYQGYIERQATEIERNDANENTRLPDGIDYREVRGLSFEVSQKL
CCHHHHHHHHHHHCCCEEEEEHHHHHHHHHHCCCCCCCCCCCCCCHHHHCCCCHHHHHHH
NQFRPETIGQASRISGVTPAAISLLMVHLKRRGLGRRNGAAAPAAEQGDGAVPTQQ
HHCCCHHCCCHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MLYPTEFDVIVVGGGHAGTEAALASARMGAKTLLLTHNIETLGQMSCNPSIGGIGKGHLV
CCCCCCCCEEEEECCCCCHHHHHHHHHCCCEEEEEEECHHHHHCCCCCCCCCCCCCCHHH
KEVDALGGAMAAATDEAGIQFRILNSSKGPAVRATRAQADRILYKAAIRRRLENQPNLWL
HHHHHHCCHHHHCCCCCCEEEEEEECCCCCCEEEHHHHHHHHHHHHHHHHHHCCCCCEEE
FQQAVDDLMVEGDCVVGAVTQIGIRFRARAVVLTAGTFLDGKIHVGLNNYTGGRAGDPAA
HHHHHHHHHCCCCEEEEHHHHHCCEEEEEEEEEEECEEECCEEEEEECCCCCCCCCCCCE
VSLSARLKELKLPQGRLKTGTPPRIDGRTIDFSKLEEQPGDLDPIPVFSFLGRAEQHPQQ
EEHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCCCCCHHHHHHCHHHCCCCC
LPCWVTHTNERTHDIIRGGLDRSPMYTGVIEGVGPRYCPSIEDKIHRFASKESHQIFLEP
CCEEEECCCCHHHHHHHCCCCCCCCHHHHHHCCCCCCCCCHHHHHHHHHCCCCCEEEECC
EGLTTNEFYPNGISTSLPFDVQLELVHSMRGLEQAHILRPGYAIEYDYFDPRALKASLET
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCHHHEECCCEEEEECCCCCHHHHHHHHH
KAINGLFFAGQINGTTGYEEAAAQGLLAGVNAGRYVQEKDAWCPRRDQAYLGVLVDDLVT
HHCCCEEEEEEECCCCCHHHHHHHHHHHCCCCCCEECCCCCCCCCCCCHHHHHHHHHHHH
RGVAEPYRMFTSRAEYRLSLREDNADMRLTEIGRELGLVDDVRWDAFCRKRDAVSRETER
HCCCHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHCCHHCCHHHHHHHHHHHHHHHHHH
LKTTWVTPKTLPPEEATALLGKPIDHEYSLAELLRRPGVDYDGVCGLKGGECGPAEPLAD
HEEEECCCCCCCHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCC
DPVLLAQIKEQVEIGIKYQGYIERQATEIERNDANENTRLPDGIDYREVRGLSFEVSQKL
CCHHHHHHHHHHHCCCEEEEEHHHHHHHHHHCCCCCCCCCCCCCCHHHHCCCCHHHHHHH
NQFRPETIGQASRISGVTPAAISLLMVHLKRRGLGRRNGAAAPAAEQGDGAVPTQQ
HHCCCHHCCCHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA