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The map label for this gene is yfeJ [H]

Identifier: 161523251

GI number: 161523251

Start: 76991

End: 77701

Strand: Direct

Name: yfeJ [H]

Synonym: Bmul_0071

Alternate gene names: 161523251

Gene position: 76991-77701 (Clockwise)

Preceding gene: 161523250

Following gene: 161523252

Centisome position: 2.23

GC content: 69.34

Gene sequence:

>711_bases
ATGAACGCTGAAGTCGTGGCGGTCCGCCACGTGCATTTCGAGGATCTCGGCAGTTTCGAGCAGGTGCTCGGCGAGCGCGG
CCGGCGGGTGCGCTACGTCGACGTCGGCTCGTCGCGCGTCGAGGTGCTCGATGCGCTCGAACCGTCGCTGCTCGTCGTGC
TCGGCGGCCCGCTCAGCGTCTACGACGACGCGCAGTATCCGACGATCGCACCGCTCGCGGCGCTCGTGAAGCAGCGGATC
GATGCCGCGCTGCCGATTCTCGGCATCTGCCTCGGCGCACAGTTCATCGCCCGCGCGCTCGGCGCGCGCGTCTATCCGGC
CGCGCAGGTCGAACTCGGCTGGTCGCCGCTGACGCTGACCGACGCCGGCCGCGCATCGCCGCTGCGTCATCTCGACGGCG
CGACGACGTCGATGCTCCACTGGCACGGCGATACGTTCGATCTGCCGGGCGGCGCGATTCATCTCGCCTCGACGCCGGCC
TGCCGGCATCAGGCATTCTCGTGGGGCCAGCATGTGCTCGCGCTGCAATGCCATCCGGAGATCCGCGCCGATCGCTTCGA
ACCCTGGCTGATCGCGAACGCGGGCGAGATCGCATCGACGCCCGGTATCGATGCGCGACAGCTGCGCGACGATACCGCGC
AGCACGGGCCCACGCTCGAAATCGCCGCGCGCACGATGTTCGGCGAATGGCTCGACAGCGTCGGGCTCTGA

Upstream 100 bases:

>100_bases
CTCGACTGTTCGATGCACGAAGAAGCCGACGTGGCCACGCAGAAAAAGACCTGGAAGATTTTCGCAGGCTGACGTCCGTC
GATCGACCGGGAGGCCGATG

Downstream 100 bases:

>100_bases
GCGGCACCGTGCCGCTCGCGCCGCTGCAGCGATCGTCGCTCGCATGCTGACCGTACGGCCCGCAAGCGGCGCGTCGGTCG
TCCCACGTTCCTGACCAATC

Product: glutamine amidotransferase

Products: p-aminobenzoate; L-glutamate; pyruvate; 4-amino-4-deoxychorismate [C]

Alternate protein names: NA

Number of amino acids: Translated: 236; Mature: 236

Protein sequence:

>236_residues
MNAEVVAVRHVHFEDLGSFEQVLGERGRRVRYVDVGSSRVEVLDALEPSLLVVLGGPLSVYDDAQYPTIAPLAALVKQRI
DAALPILGICLGAQFIARALGARVYPAAQVELGWSPLTLTDAGRASPLRHLDGATTSMLHWHGDTFDLPGGAIHLASTPA
CRHQAFSWGQHVLALQCHPEIRADRFEPWLIANAGEIASTPGIDARQLRDDTAQHGPTLEIAARTMFGEWLDSVGL

Sequences:

>Translated_236_residues
MNAEVVAVRHVHFEDLGSFEQVLGERGRRVRYVDVGSSRVEVLDALEPSLLVVLGGPLSVYDDAQYPTIAPLAALVKQRI
DAALPILGICLGAQFIARALGARVYPAAQVELGWSPLTLTDAGRASPLRHLDGATTSMLHWHGDTFDLPGGAIHLASTPA
CRHQAFSWGQHVLALQCHPEIRADRFEPWLIANAGEIASTPGIDARQLRDDTAQHGPTLEIAARTMFGEWLDSVGL
>Mature_236_residues
MNAEVVAVRHVHFEDLGSFEQVLGERGRRVRYVDVGSSRVEVLDALEPSLLVVLGGPLSVYDDAQYPTIAPLAALVKQRI
DAALPILGICLGAQFIARALGARVYPAAQVELGWSPLTLTDAGRASPLRHLDGATTSMLHWHGDTFDLPGGAIHLASTPA
CRHQAFSWGQHVLALQCHPEIRADRFEPWLIANAGEIASTPGIDARQLRDDTAQHGPTLEIAARTMFGEWLDSVGL

Specific function: Catalyzes The Biosynthesis Of 4-Amino-4-Deoxychorismate (Adc) From Chorismate And Glutamine. [C]

COG id: COG0518

COG function: function code F; GMP synthase - Glutamine amidotransferase domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017926
- InterPro:   IPR000991 [H]

Pfam domain/function: PF00117 GATase [H]

EC number: 6.3.5.8 [C]

Molecular weight: Translated: 25504; Mature: 25504

Theoretical pI: Translated: 5.74; Mature: 5.74

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNAEVVAVRHVHFEDLGSFEQVLGERGRRVRYVDVGSSRVEVLDALEPSLLVVLGGPLSV
CCCCEEEEEEECHHHCCHHHHHHHHCCCEEEEEECCCCHHHHHHHCCCCEEEEECCCEEE
YDDAQYPTIAPLAALVKQRIDAALPILGICLGAQFIARALGARVYPAAQVELGWSPLTLT
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCEEEC
DAGRASPLRHLDGATTSMLHWHGDTFDLPGGAIHLASTPACRHQAFSWGQHVLALQCHPE
CCCCCCCHHHCCCCCEEEEEECCCCEECCCCEEEECCCCHHHHHHHHCCCEEEEEEECCC
IRADRFEPWLIANAGEIASTPGIDARQLRDDTAQHGPTLEIAARTMFGEWLDSVGL
CCCCCCCCEEEECCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNAEVVAVRHVHFEDLGSFEQVLGERGRRVRYVDVGSSRVEVLDALEPSLLVVLGGPLSV
CCCCEEEEEEECHHHCCHHHHHHHHCCCEEEEEECCCCHHHHHHHCCCCEEEEECCCEEE
YDDAQYPTIAPLAALVKQRIDAALPILGICLGAQFIARALGARVYPAAQVELGWSPLTLT
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCEEEC
DAGRASPLRHLDGATTSMLHWHGDTFDLPGGAIHLASTPACRHQAFSWGQHVLALQCHPE
CCCCCCCHHHCCCCCEEEEEECCCCEECCCCEEEECCCCHHHHHHHHCCCEEEEEEECCC
IRADRFEPWLIANAGEIASTPGIDARQLRDDTAQHGPTLEIAARTMFGEWLDSVGL
CCCCCCCCEEEECCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-glutamine; chorismate [C]

Specific reaction: L-glutamine + chorismate = p-aminobenzoate + L-glutamate + pyruvate L-glutamine + chorismate = 4-amino-4-deoxychorismate + L-glutamate [C]

General reaction: Lyases; Carbon-Carbon Lyases; Oxo-Acid-Lyases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7612925; 11677609 [H]