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The map label for this gene is nicK [H]

Identifier: 161508878

GI number: 161508878

Start: 691882

End: 692973

Strand: Reverse

Name: nicK [H]

Synonym: USA300HOU_0635

Alternate gene names: 161508878

Gene position: 692973-691882 (Counterclockwise)

Preceding gene: 161508879

Following gene: 161508877

Centisome position: 24.12

GC content: 32.97

Gene sequence:

>1092_bases
ATGACTTTAAAAAACCAGTGTACCCCCCTTACTAATAGGGGGGTAGAGCGTACAAATAAAAGTGCTGTGGAGGCAGTTGT
TGACTGGGTTCAGGTCACTTTCCATATAGCCCCTATTTCAGCAGTAATTGAGGACGTTATCGGTTTGCCTATAACGTTGT
TCAAAAAACGAAATAGTGGCATTTACTTTTATAATCGCGGTTATGAATTTTCAAACATTAAATTGTATTATTCAAGTGAT
GATGAATCAATGGGCATTCACTTACAGTTAACTGGTACAGGTTGTAGAGAGTTTGAACATCATTTACAGCAGTTGAATAA
AACTTGGCAAGATTTTTTTGACAAATGTTTATCAGTTAATGCAAATTTTACAAGAATTGATATTGCAATTGATGATTATA
AAACATATTTAAAAGTGCCTTTATTAATTAAAAAGGCTGAAAAAGCTGAGTGTGTTTCACGTTTTAGAGCTGGAAGCGCA
ATAAACGGTTTTAATTTGTCAGACGGAAGAAGTAAAGGCGCTACTTTTTATATTGGTTCTAAACAAAGCAATTTATATTG
TCGTTTTTATGAAAAGAATTATGAACAAGCGTTTAAACGTCATTGTGATGTCGAAGACATTGGGCTATGGAATCGTTATG
AAATACAAATGCGAAAGGCATATGCAGTTAATTGTGCGAAAGTGTTGAGTAGAACAGATAATATAAGCGAAATTGTAAAG
TCTATATTGCATAATAATTTGCGTTTTATCTCTCCTCCTAAAGGTGGAAATGATAAAAATCGTAAGCGGTGGCCATTATA
TAGACCTTGGGCGTTATTTATTAAAGATACTGAAAAATTGAATTTAACTACTAGACCAACATTAAAGTCCATTGAGGATA
ATCTTGATTGGTTATGTAAACAAGTTGCTACTACTTTAGATACAGTGTTAACTGCCGAAAGTATGGCGCAATCTGAGGGG
TTGCTTACAGATACTGATTTTTTAGATAAAATTTTAGCGCATTCACAATTTAATGATGAGCATACGAATAGAATTAATCA
TTATTTGGAGGCTTTAAAACAAAAAAAGCACTTATCAAAAGATAAGTGTTAA

Upstream 100 bases:

>100_bases
CGTTAAGCATTTGGGCGGAGCCTGCGACAAATACGCGTTAAAAAAGCGAGAACGGTCATGTATCAAATCGCTTGCGATTT
GATAGGAGGCATAAATAAAT

Downstream 100 bases:

>100_bases
AAAAATAACTGGGATGTGATTTTTATTAAAAATTTCGTAAAGGATTTTAGAAAATCGTTTATGAAAATAATAAGCAAGTA
CATTTTAACACGATTTTCTA

Product: replication initiation protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 363; Mature: 362

Protein sequence:

>363_residues
MTLKNQCTPLTNRGVERTNKSAVEAVVDWVQVTFHIAPISAVIEDVIGLPITLFKKRNSGIYFYNRGYEFSNIKLYYSSD
DESMGIHLQLTGTGCREFEHHLQQLNKTWQDFFDKCLSVNANFTRIDIAIDDYKTYLKVPLLIKKAEKAECVSRFRAGSA
INGFNLSDGRSKGATFYIGSKQSNLYCRFYEKNYEQAFKRHCDVEDIGLWNRYEIQMRKAYAVNCAKVLSRTDNISEIVK
SILHNNLRFISPPKGGNDKNRKRWPLYRPWALFIKDTEKLNLTTRPTLKSIEDNLDWLCKQVATTLDTVLTAESMAQSEG
LLTDTDFLDKILAHSQFNDEHTNRINHYLEALKQKKHLSKDKC

Sequences:

>Translated_363_residues
MTLKNQCTPLTNRGVERTNKSAVEAVVDWVQVTFHIAPISAVIEDVIGLPITLFKKRNSGIYFYNRGYEFSNIKLYYSSD
DESMGIHLQLTGTGCREFEHHLQQLNKTWQDFFDKCLSVNANFTRIDIAIDDYKTYLKVPLLIKKAEKAECVSRFRAGSA
INGFNLSDGRSKGATFYIGSKQSNLYCRFYEKNYEQAFKRHCDVEDIGLWNRYEIQMRKAYAVNCAKVLSRTDNISEIVK
SILHNNLRFISPPKGGNDKNRKRWPLYRPWALFIKDTEKLNLTTRPTLKSIEDNLDWLCKQVATTLDTVLTAESMAQSEG
LLTDTDFLDKILAHSQFNDEHTNRINHYLEALKQKKHLSKDKC
>Mature_362_residues
TLKNQCTPLTNRGVERTNKSAVEAVVDWVQVTFHIAPISAVIEDVIGLPITLFKKRNSGIYFYNRGYEFSNIKLYYSSDD
ESMGIHLQLTGTGCREFEHHLQQLNKTWQDFFDKCLSVNANFTRIDIAIDDYKTYLKVPLLIKKAEKAECVSRFRAGSAI
NGFNLSDGRSKGATFYIGSKQSNLYCRFYEKNYEQAFKRHCDVEDIGLWNRYEIQMRKAYAVNCAKVLSRTDNISEIVKS
ILHNNLRFISPPKGGNDKNRKRWPLYRPWALFIKDTEKLNLTTRPTLKSIEDNLDWLCKQVATTLDTVLTAESMAQSEGL
LTDTDFLDKILAHSQFNDEHTNRINHYLEALKQKKHLSKDKC

Specific function: Probable DNA relaxase involved in the transfer of the integrative and conjugative element ICEBs1. Required for the transfer of ICEBs1. Probably mediates conjugation of ICEBs1 by nicking at oriT on the conjugative element and facilitates the translocation

COG id: COG2946

COG function: function code L; Putative phage replication protein RstA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the plasmid replication initiation factor family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003491 [H]

Pfam domain/function: PF02486 Rep_trans [H]

EC number: NA

Molecular weight: Translated: 41953; Mature: 41822

Theoretical pI: Translated: 8.98; Mature: 8.98

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLKNQCTPLTNRGVERTNKSAVEAVVDWVQVTFHIAPISAVIEDVIGLPITLFKKRNSG
CCCCCCCCCCCCCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCC
IYFYNRGYEFSNIKLYYSSDDESMGIHLQLTGTGCREFEHHLQQLNKTWQDFFDKCLSVN
EEEEECCEEECEEEEEECCCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCC
ANFTRIDIAIDDYKTYLKVPLLIKKAEKAECVSRFRAGSAINGFNLSDGRSKGATFYIGS
CCEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEC
KQSNLYCRFYEKNYEQAFKRHCDVEDIGLWNRYEIQMRKAYAVNCAKVLSRTDNISEIVK
CCCCEEEEEEHHHHHHHHHHCCCHHHCCCCCHHHEEEHHHHHHHHHHHHHCCCCHHHHHH
SILHNNLRFISPPKGGNDKNRKRWPLYRPWALFIKDTEKLNLTTRPTLKSIEDNLDWLCK
HHHHCCCEEECCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHH
QVATTLDTVLTAESMAQSEGLLTDTDFLDKILAHSQFNDEHTNRINHYLEALKQKKHLSK
HHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCC
DKC
CCC
>Mature Secondary Structure 
TLKNQCTPLTNRGVERTNKSAVEAVVDWVQVTFHIAPISAVIEDVIGLPITLFKKRNSG
CCCCCCCCCCCCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCC
IYFYNRGYEFSNIKLYYSSDDESMGIHLQLTGTGCREFEHHLQQLNKTWQDFFDKCLSVN
EEEEECCEEECEEEEEECCCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCC
ANFTRIDIAIDDYKTYLKVPLLIKKAEKAECVSRFRAGSAINGFNLSDGRSKGATFYIGS
CCEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEC
KQSNLYCRFYEKNYEQAFKRHCDVEDIGLWNRYEIQMRKAYAVNCAKVLSRTDNISEIVK
CCCCEEEEEEHHHHHHHHHHCCCHHHCCCCCHHHEEEHHHHHHHHHHHHHCCCCHHHHHH
SILHNNLRFISPPKGGNDKNRKRWPLYRPWALFIKDTEKLNLTTRPTLKSIEDNLDWLCK
HHHHCCCEEECCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHH
QVATTLDTVLTAESMAQSEGLLTDTDFLDKILAHSQFNDEHTNRINHYLEALKQKKHLSK
HHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCC
DKC
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]