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The map label for this gene is purF [H]

Identifier: 160903389

GI number: 160903389

Start: 2168877

End: 2169353

Strand: Direct

Name: purF [H]

Synonym: Pmob_1960

Alternate gene names: 160903389

Gene position: 2168877-2169353 (Clockwise)

Preceding gene: 160903388

Following gene: NA

Centisome position: 99.97

GC content: 37.74

Gene sequence:

>477_bases
ATGTTGATGGAAAATTGTGGTTTGTTTGCCGCATATTCAAAGAGTCAAAAGTACAACGTAAGTGGCAGAATTGTGGAAGG
TCTTTTGGCACTTCAACATAGAGGCCAAGAATCAGCCGGGATCTCGGTGTCAGATGGTACTAAAATTACAACTTATAAGG
GTAAAGGCGTAGTCAATAGAGTTTTCGGGGGCGGTGTATCAAAAAAAATTAACGGTTATTTTGGGATCGGGCACGTAAGA
TATTCAACGAAAGGGGTCTCTAATTTCACAAATGCTCAACCGCTTACTATAAAGTATAAAAATGAATTTTTTTCCATAGC
TCACAACGGTCAAATTGAAAATGGTCCTGAAATGAAAGAGAAATACGAAGAGCAAGGCTCCATATTTATGACAACTACAG
ATACGGAACTTTTTCCCCATCTTTTAGTTAAGAAGCTAAAAGGCTCTCCTTCAAGTTGGAAAAGTGAAGAAATTTAA

Upstream 100 bases:

>100_bases
AGTGATGACGGTATCGATATCTGCAGTGCATATTTTGATTTAAAAGAATTGAAAAGTATCTATTTTGATAGTATCTCGAA
AAGTGTGGAGGAATAATAGA

Downstream 100 bases:

>100_bases
CATTTCTTATTATTCAAAAATTTATACGTTTGTATATTATACATTTTTATTTTTTAAAACAAAGATTTAACAATTTTTGT
TCTTCAAGTTGTCTCTTAAA

Product: glutamine amidotransferase class-II

Products: NA

Alternate protein names: ATase; Glutamine phosphoribosylpyrophosphate amidotransferase; GPATase [H]

Number of amino acids: Translated: 158; Mature: 158

Protein sequence:

>158_residues
MLMENCGLFAAYSKSQKYNVSGRIVEGLLALQHRGQESAGISVSDGTKITTYKGKGVVNRVFGGGVSKKINGYFGIGHVR
YSTKGVSNFTNAQPLTIKYKNEFFSIAHNGQIENGPEMKEKYEEQGSIFMTTTDTELFPHLLVKKLKGSPSSWKSEEI

Sequences:

>Translated_158_residues
MLMENCGLFAAYSKSQKYNVSGRIVEGLLALQHRGQESAGISVSDGTKITTYKGKGVVNRVFGGGVSKKINGYFGIGHVR
YSTKGVSNFTNAQPLTIKYKNEFFSIAHNGQIENGPEMKEKYEEQGSIFMTTTDTELFPHLLVKKLKGSPSSWKSEEI
>Mature_158_residues
MLMENCGLFAAYSKSQKYNVSGRIVEGLLALQHRGQESAGISVSDGTKITTYKGKGVVNRVFGGGVSKKINGYFGIGHVR
YSTKGVSNFTNAQPLTIKYKNEFFSIAHNGQIENGPEMKEKYEEQGSIFMTTTDTELFPHLLVKKLKGSPSSWKSEEI

Specific function: De novo purine biosynthesis; first step. [C]

COG id: COG0034

COG function: function code F; Glutamine phosphoribosylpyrophosphate amidotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI29570798, Length=148, Percent_Identity=37.1621621621622, Blast_Score=99, Evalue=1e-21,
Organism=Escherichia coli, GI1788651, Length=131, Percent_Identity=37.4045801526718, Blast_Score=82, Evalue=2e-17,
Organism=Escherichia coli, GI1790167, Length=143, Percent_Identity=32.1678321678322, Blast_Score=75, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17554892, Length=147, Percent_Identity=31.9727891156463, Blast_Score=71, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17532899, Length=154, Percent_Identity=29.2207792207792, Blast_Score=66, Evalue=6e-12,
Organism=Caenorhabditis elegans, GI17532897, Length=154, Percent_Identity=29.2207792207792, Blast_Score=66, Evalue=6e-12,
Organism=Caenorhabditis elegans, GI17539970, Length=155, Percent_Identity=31.6129032258064, Blast_Score=65, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6323958, Length=146, Percent_Identity=32.1917808219178, Blast_Score=70, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24659598, Length=143, Percent_Identity=37.7622377622378, Blast_Score=100, Evalue=4e-22,
Organism=Drosophila melanogaster, GI28573187, Length=147, Percent_Identity=36.0544217687075, Blast_Score=98, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24659604, Length=123, Percent_Identity=39.8373983739837, Blast_Score=94, Evalue=3e-20,
Organism=Drosophila melanogaster, GI21357745, Length=153, Percent_Identity=29.4117647058824, Blast_Score=64, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005854
- InterPro:   IPR000583
- InterPro:   IPR017932
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00310 GATase_2; PF00156 Pribosyltran [H]

EC number: =2.4.2.14 [H]

Molecular weight: Translated: 17422; Mature: 17422

Theoretical pI: Translated: 9.84; Mature: 9.84

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLMENCGLFAAYSKSQKYNVSGRIVEGLLALQHRGQESAGISVSDGTKITTYKGKGVVNR
CCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHCCCCCCCEEECCCCEEEEEECCCCEEH
VFGGGVSKKINGYFGIGHVRYSTKGVSNFTNAQPLTIKYKNEFFSIAHNGQIENGPEMKE
HHCCCCCCCCCCEEEEEEEEEECCCCCCCCCCCCEEEEECCCEEEEEECCCCCCCCHHHH
KYEEQGSIFMTTTDTELFPHLLVKKLKGSPSSWKSEEI
HHHHCCCEEEEECCHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MLMENCGLFAAYSKSQKYNVSGRIVEGLLALQHRGQESAGISVSDGTKITTYKGKGVVNR
CCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHCCCCCCCEEECCCCEEEEEECCCCEEH
VFGGGVSKKINGYFGIGHVRYSTKGVSNFTNAQPLTIKYKNEFFSIAHNGQIENGPEMKE
HHCCCCCCCCCCEEEEEEEEEECCCCCCCCCCCCEEEEECCCEEEEEECCCCCCCCHHHH
KYEEQGSIFMTTTDTELFPHLLVKKLKGSPSSWKSEEI
HHHHCCCEEEEECCHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]