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The map label for this gene is pcm

Identifier: 160903330

GI number: 160903330

Start: 2105473

End: 2106114

Strand: Direct

Name: pcm

Synonym: Pmob_1901

Alternate gene names: 160903330

Gene position: 2105473-2106114 (Clockwise)

Preceding gene: 160903329

Following gene: 160903332

Centisome position: 97.05

GC content: 35.51

Gene sequence:

>642_bases
ATGGATTTTGAGAAAGAAAGTAGAATGATGGTTGAGTATCAGCTAAAAAGAAGAGGTATAAGCGATGAAAAGGTTCTAAA
TGCTTTTTTGAAGGTTAAAAGACATTTGTTTGTACCAAAAGATCTTGAGAGGTATGCATATGATGATTGCCCTTTGCCTA
TTGGCGAAGGGCAAACTATTTCTCAGCCTTATATAATCGGCTTAATGCTTCAATTATTAGAGTTAAGAGAAAACGACGTA
GTTTTAGAGATAGGAACGGGATCTGGTTATCAAACTGCTTTACTTGCAGAGATAGTGCGTCTTGTTTATACGATAGAACG
AAATGAAACGTTAGCCCAAAGAGCAAAGAATAAATTTGAGGAATTAGGTTATAAAAATATTGTTTTAGAGGTTGGAGATG
GAACAAAAGGGTGGACAAAAGAAGAGATTGAGTTTGACGGAATCATAGTTTCAGCGGCAGCTCCAAAGGTTCCTGAACCT
TTGTTTTCTCAACTTAAAATTGGTGGTAGAATGGTAATTCCAATTGGTTCTAGAACCTTTCAACGTTTACATAAAATAAC
TAAGTTAGAGGATGGAAATATGAAGGTTGAATACTCTGATGGATGCATGTTTGTTCCTTTAATAGGTGAATATGGTTGGT
GA

Upstream 100 bases:

>100_bases
TAGTTACAGGTGCAGCAACCGTTATCGAGGCAATGGGTGCAGGTAAAAGAGCTGCTAAGGCAATAGATGAATATATTTCT
TCTAAGGTAGGAAAATTTTG

Downstream 100 bases:

>100_bases
GAGTGTTTAGTTAAAATATAAAATATCCCGCAAAAAGCGGGATATTTTAATTATTAATATGGAAAAGTTATCTCAGGAAC
TTCAAATGATCCAATTTGGG

Product: protein-L-isoaspartate O-methyltransferase

Products: NA

Alternate protein names: L-isoaspartyl protein carboxyl methyltransferase; Protein L-isoaspartyl methyltransferase; Protein-beta-aspartate methyltransferase; PIMT

Number of amino acids: Translated: 213; Mature: 213

Protein sequence:

>213_residues
MDFEKESRMMVEYQLKRRGISDEKVLNAFLKVKRHLFVPKDLERYAYDDCPLPIGEGQTISQPYIIGLMLQLLELRENDV
VLEIGTGSGYQTALLAEIVRLVYTIERNETLAQRAKNKFEELGYKNIVLEVGDGTKGWTKEEIEFDGIIVSAAAPKVPEP
LFSQLKIGGRMVIPIGSRTFQRLHKITKLEDGNMKVEYSDGCMFVPLIGEYGW

Sequences:

>Translated_213_residues
MDFEKESRMMVEYQLKRRGISDEKVLNAFLKVKRHLFVPKDLERYAYDDCPLPIGEGQTISQPYIIGLMLQLLELRENDV
VLEIGTGSGYQTALLAEIVRLVYTIERNETLAQRAKNKFEELGYKNIVLEVGDGTKGWTKEEIEFDGIIVSAAAPKVPEP
LFSQLKIGGRMVIPIGSRTFQRLHKITKLEDGNMKVEYSDGCMFVPLIGEYGW
>Mature_213_residues
MDFEKESRMMVEYQLKRRGISDEKVLNAFLKVKRHLFVPKDLERYAYDDCPLPIGEGQTISQPYIIGLMLQLLELRENDV
VLEIGTGSGYQTALLAEIVRLVYTIERNETLAQRAKNKFEELGYKNIVLEVGDGTKGWTKEEIEFDGIIVSAAAPKVPEP
LFSQLKIGGRMVIPIGSRTFQRLHKITKLEDGNMKVEYSDGCMFVPLIGEYGW

Specific function: Catalyzes the methyl esterification of L-isoaspartyl residues in peptides and proteins that result from spontaneous decomposition of normal L-aspartyl and L-asparaginyl residues. It plays a role in the repair and/or degradation of damaged proteins

COG id: COG2518

COG function: function code O; Protein-L-isoaspartate carboxylmethyltransferase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. L- isoaspartyl/D-aspartyl protein methyltransferase family

Homologues:

Organism=Homo sapiens, GI226530908, Length=207, Percent_Identity=34.7826086956522, Blast_Score=97, Evalue=1e-20,
Organism=Escherichia coli, GI1789100, Length=195, Percent_Identity=44.1025641025641, Blast_Score=162, Evalue=1e-41,
Organism=Caenorhabditis elegans, GI71983477, Length=206, Percent_Identity=30.0970873786408, Blast_Score=82, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI193207222, Length=209, Percent_Identity=28.7081339712919, Blast_Score=70, Evalue=7e-13,
Organism=Drosophila melanogaster, GI17981723, Length=216, Percent_Identity=32.8703703703704, Blast_Score=93, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PIMT_PETMO (A9BH07)

Other databases:

- EMBL:   CP000879
- RefSeq:   YP_001568911.1
- ProteinModelPortal:   A9BH07
- SMR:   A9BH07
- GeneID:   5758090
- GenomeReviews:   CP000879_GR
- KEGG:   pmo:Pmob_1901
- HOGENOM:   HBG699907
- OMA:   GYHAAVV
- BioCyc:   PMOB403833:PMOB_1901-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00090
- InterPro:   IPR000682
- PANTHER:   PTHR11579
- TIGRFAMs:   TIGR00080

Pfam domain/function: PF01135 PCMT

EC number: =2.1.1.77

Molecular weight: Translated: 24280; Mature: 24280

Theoretical pI: Translated: 5.21; Mature: 5.21

Prosite motif: PS01279 PCMT

Important sites: ACT_SITE 61-61

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDFEKESRMMVEYQLKRRGISDEKVLNAFLKVKRHLFVPKDLERYAYDDCPLPIGEGQTI
CCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCCCCCCCCCC
SQPYIIGLMLQLLELRENDVVLEIGTGSGYQTALLAEIVRLVYTIERNETLAQRAKNKFE
CCCHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
ELGYKNIVLEVGDGTKGWTKEEIEFDGIIVSAAAPKVPEPLFSQLKIGGRMVIPIGSRTF
HCCCEEEEEECCCCCCCCCCCCEEECCEEEECCCCCCCHHHHHHHHCCCEEEEEECHHHH
QRLHKITKLEDGNMKVEYSDGCMFVPLIGEYGW
HHHHHHHCCCCCCEEEEECCCEEEEEECCCCCC
>Mature Secondary Structure
MDFEKESRMMVEYQLKRRGISDEKVLNAFLKVKRHLFVPKDLERYAYDDCPLPIGEGQTI
CCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCCCCCCCCCC
SQPYIIGLMLQLLELRENDVVLEIGTGSGYQTALLAEIVRLVYTIERNETLAQRAKNKFE
CCCHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
ELGYKNIVLEVGDGTKGWTKEEIEFDGIIVSAAAPKVPEPLFSQLKIGGRMVIPIGSRTF
HCCCEEEEEECCCCCCCCCCCCEEECCEEEECCCCCCCHHHHHHHHCCCEEEEEECHHHH
QRLHKITKLEDGNMKVEYSDGCMFVPLIGEYGW
HHHHHHHCCCCCCEEEEECCCEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA