Click here to switch to the map view.

The map label for this gene is gltB [H]

Identifier: 160903226

GI number: 160903226

Start: 1970932

End: 1972452

Strand: Direct

Name: gltB [H]

Synonym: Pmob_1795

Alternate gene names: 160903226

Gene position: 1970932-1972452 (Clockwise)

Preceding gene: 160903225

Following gene: 160903227

Centisome position: 90.85

GC content: 36.82

Gene sequence:

>1521_bases
ATGGGCGATCCCCAAGGATTTCTAAAGTATGAAAGAAAAACCCCTGAAGAAAGACCCTCAAAAATAAGAATAAATGATTG
GGAAGAATTATATATAGAAATGGAAAAAGATGAACTTCGAATGCAAGCAGCAAGATGCATGAACTGCGGCACACCGTTTT
GTCATTCTGGCATATTGATAAATAATATGGTCTCGGGGTGCCCTTTGAATAATTTGATTCCAGAATGGAACGATTTAGTA
TATAGAAACCTTTGGAAAGAAGCTTACGAAAGGTTGATCGAAACAAACAGTTTCCCAGAATTCACGGGAAGGGTTTGTCC
TGCTCCATGCGAAAAAGCTTGTGTAAACAATTTGGTAAAGGATCCGGTTTCCATTAAAAGTATTGAATATTATATTATAG
AAGAAGCATTCAAAAATGGTTGGGTAGAAGAGGATAAGCCATCTTACTCCACTGGTAAAAAGGTGGCTGTTGTAGGTTCT
GGACCGGCAGGGTTATCATGTGCTTGGGAGTTAAAAAGATATGGTCATGAAGTTACAGTTTTTGAAAAGTATGAATATCC
GGGGGGCTTATTGACTTACGGTATACCAAATATGAAATTGGACAAAAAGATTGTAAAAAGACGTATAGAATTGATGAAAA
AATCTGGCATACAATTCAAAACTAATATAGACGTTGGTAAAGATTATCCTGCTCAAAAGCTAAAAGAAGAGTTTGATAGC
GTTGTATTATGTGGAGGAACCGCTAAAGCGAGAGATTTAAACGTAGAGGGAAGAAACCTAAAAGGGATATACTTTGCCAC
AGAATTTTTGAAGAGTGCAAACGAGAGAATACTGTCTCAATTTATAAAAAGTGAAAAAGATATTGAGGAGATCTCTGCTA
AGGGTAAGAACGTTATCGTAATCGGTGGAGGAGATACGGGAACGGATTGTGTGGGAGTTAGTTTAAGACAGGGTTGTAAA
AGTGTTGTACAGTTTGAAATTATGGACAAACCTCCACTTTTAAGGAGACAAAACAATCCTTGGCCAGAATGGCCCAAAGT
TTTAACTGTTGATTATGCTCAGGAAGAATATGCAGAACTATTCGGTAAAGATCCGAGATTGTACAACATTTCAACTAAAA
GATTTGTTGGAGATGCAAAAGGTAACGTTAAAGAGGTCCATACCGTTGAAGTAGAATGGAAACAGGATAAAACAGGAAAA
TTGTTTCCAAAAGAAATCGATGGAACAGAAAAGGTATGGAAAGCAGATTTAGTTCTGTTAGCCTTGGGATTCTTAGGCCC
CGAAAAGTATTTAATTGAGCAGTTAAATGTAGAAACAGATAAAAGATCAAACGTTAAAACGGAAGAAGGAAAATACTCGA
CAAATGTGGAAGGGATTTTTTCAGCTGGAGATATGAGAAGGGGACAAAGTTTGGTAGTATGGGCAATAAATGAAGGGAAA
CAAGCAGCAAAAGAATGTAATGAATACTTAATTTCTTCCAATAATAACAATAATAAACAGCGTCGGCTCAAAACGCTGTA
G

Upstream 100 bases:

>100_bases
TGGTGAAGAAGCACTTATGAAGGCTTTTGAAGATAATTACAAGCAACAATCTTTGGTTGAAATGCATTGAACATTCGCAA
TTTGTAAAGGAGGTCACTAT

Downstream 100 bases:

>100_bases
TATTGTTGTTATAAAGCCGACGCAGAAAAATTAATGCGTCGGTTTTTTATTTTGTAATGGGAGGTGAAAAAAGTCTACGT
TAAGTACCATATTCTGGGGT

Product: glutamate synthase, NADH/NADPH, small subunit

Products: NA

Alternate protein names: NADPH-GOGAT [H]

Number of amino acids: Translated: 506; Mature: 505

Protein sequence:

>506_residues
MGDPQGFLKYERKTPEERPSKIRINDWEELYIEMEKDELRMQAARCMNCGTPFCHSGILINNMVSGCPLNNLIPEWNDLV
YRNLWKEAYERLIETNSFPEFTGRVCPAPCEKACVNNLVKDPVSIKSIEYYIIEEAFKNGWVEEDKPSYSTGKKVAVVGS
GPAGLSCAWELKRYGHEVTVFEKYEYPGGLLTYGIPNMKLDKKIVKRRIELMKKSGIQFKTNIDVGKDYPAQKLKEEFDS
VVLCGGTAKARDLNVEGRNLKGIYFATEFLKSANERILSQFIKSEKDIEEISAKGKNVIVIGGGDTGTDCVGVSLRQGCK
SVVQFEIMDKPPLLRRQNNPWPEWPKVLTVDYAQEEYAELFGKDPRLYNISTKRFVGDAKGNVKEVHTVEVEWKQDKTGK
LFPKEIDGTEKVWKADLVLLALGFLGPEKYLIEQLNVETDKRSNVKTEEGKYSTNVEGIFSAGDMRRGQSLVVWAINEGK
QAAKECNEYLISSNNNNNKQRRLKTL

Sequences:

>Translated_506_residues
MGDPQGFLKYERKTPEERPSKIRINDWEELYIEMEKDELRMQAARCMNCGTPFCHSGILINNMVSGCPLNNLIPEWNDLV
YRNLWKEAYERLIETNSFPEFTGRVCPAPCEKACVNNLVKDPVSIKSIEYYIIEEAFKNGWVEEDKPSYSTGKKVAVVGS
GPAGLSCAWELKRYGHEVTVFEKYEYPGGLLTYGIPNMKLDKKIVKRRIELMKKSGIQFKTNIDVGKDYPAQKLKEEFDS
VVLCGGTAKARDLNVEGRNLKGIYFATEFLKSANERILSQFIKSEKDIEEISAKGKNVIVIGGGDTGTDCVGVSLRQGCK
SVVQFEIMDKPPLLRRQNNPWPEWPKVLTVDYAQEEYAELFGKDPRLYNISTKRFVGDAKGNVKEVHTVEVEWKQDKTGK
LFPKEIDGTEKVWKADLVLLALGFLGPEKYLIEQLNVETDKRSNVKTEEGKYSTNVEGIFSAGDMRRGQSLVVWAINEGK
QAAKECNEYLISSNNNNNKQRRLKTL
>Mature_505_residues
GDPQGFLKYERKTPEERPSKIRINDWEELYIEMEKDELRMQAARCMNCGTPFCHSGILINNMVSGCPLNNLIPEWNDLVY
RNLWKEAYERLIETNSFPEFTGRVCPAPCEKACVNNLVKDPVSIKSIEYYIIEEAFKNGWVEEDKPSYSTGKKVAVVGSG
PAGLSCAWELKRYGHEVTVFEKYEYPGGLLTYGIPNMKLDKKIVKRRIELMKKSGIQFKTNIDVGKDYPAQKLKEEFDSV
VLCGGTAKARDLNVEGRNLKGIYFATEFLKSANERILSQFIKSEKDIEEISAKGKNVIVIGGGDTGTDCVGVSLRQGCKS
VVQFEIMDKPPLLRRQNNPWPEWPKVLTVDYAQEEYAELFGKDPRLYNISTKRFVGDAKGNVKEVHTVEVEWKQDKTGKL
FPKEIDGTEKVWKADLVLLALGFLGPEKYLIEQLNVETDKRSNVKTEEGKYSTNVEGIFSAGDMRRGQSLVVWAINEGKQ
AAKECNEYLISSNNNNNKQRRLKTL

Specific function: NITROGEN METABOLISM, GLUTAMATE BIOSYNTHESIS. THE CATALYZED REACTION BRINGS TOGETHER THE NITROGEN AND CARBON METABOLISM. [C]

COG id: COG0493

COG function: function code ER; NADPH-dependent glutamate synthase beta chain and related oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamate synthase family [H]

Homologues:

Organism=Homo sapiens, GI119943098, Length=478, Percent_Identity=26.5690376569038, Blast_Score=100, Evalue=3e-21,
Organism=Escherichia coli, GI1789606, Length=483, Percent_Identity=35.6107660455487, Blast_Score=265, Evalue=5e-72,
Organism=Escherichia coli, GI1788811, Length=485, Percent_Identity=33.1958762886598, Blast_Score=241, Evalue=5e-65,
Organism=Escherichia coli, GI87082180, Length=484, Percent_Identity=30.9917355371901, Blast_Score=211, Evalue=1e-55,
Organism=Escherichia coli, GI1788468, Length=452, Percent_Identity=25.2212389380531, Blast_Score=127, Evalue=1e-30,
Organism=Escherichia coli, GI1789243, Length=429, Percent_Identity=26.1072261072261, Blast_Score=108, Evalue=9e-25,
Organism=Caenorhabditis elegans, GI17570289, Length=494, Percent_Identity=48.7854251012146, Blast_Score=501, Evalue=1e-142,
Organism=Caenorhabditis elegans, GI71984108, Length=496, Percent_Identity=24.1935483870968, Blast_Score=99, Evalue=6e-21,
Organism=Caenorhabditis elegans, GI17543860, Length=164, Percent_Identity=27.4390243902439, Blast_Score=71, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6320030, Length=490, Percent_Identity=46.9387755102041, Blast_Score=480, Evalue=1e-136,
Organism=Drosophila melanogaster, GI28574881, Length=487, Percent_Identity=52.3613963039014, Blast_Score=530, Evalue=1e-151,
Organism=Drosophila melanogaster, GI24665539, Length=487, Percent_Identity=52.3613963039014, Blast_Score=530, Evalue=1e-151,
Organism=Drosophila melanogaster, GI24665547, Length=487, Percent_Identity=52.3613963039014, Blast_Score=528, Evalue=1e-150,
Organism=Drosophila melanogaster, GI24665543, Length=487, Percent_Identity=52.3613963039014, Blast_Score=528, Evalue=1e-150,
Organism=Drosophila melanogaster, GI24640763, Length=482, Percent_Identity=24.6887966804979, Blast_Score=96, Evalue=8e-20,
Organism=Drosophila melanogaster, GI18858217, Length=482, Percent_Identity=24.6887966804979, Blast_Score=96, Evalue=8e-20,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013027
- InterPro:   IPR012285
- InterPro:   IPR006005
- InterPro:   IPR009051
- InterPro:   IPR016040
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: =1.4.1.13 [H]

Molecular weight: Translated: 57514; Mature: 57383

Theoretical pI: Translated: 7.77; Mature: 7.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGDPQGFLKYERKTPEERPSKIRINDWEELYIEMEKDELRMQAARCMNCGTPFCHSGILI
CCCCHHHHHHHHCCCCCCCCEEEECCHHHEEEEECHHHHHHHHHHHHCCCCCHHHCCEEH
NNMVSGCPLNNLIPEWNDLVYRNLWKEAYERLIETNSFPEFTGRVCPAPCEKACVNNLVK
HHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHCCCCCCCCHHHHHHHHHHC
DPVSIKSIEYYIIEEAFKNGWVEEDKPSYSTGKKVAVVGSGPAGLSCAWELKRYGHEVTV
CCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHCCCEEEE
FEKYEYPGGLLTYGIPNMKLDKKIVKRRIELMKKSGIQFKTNIDVGKDYPAQKLKEEFDS
EEEECCCCCEEEECCCCCCHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCHHHHHHHHCC
VVLCGGTAKARDLNVEGRNLKGIYFATEFLKSANERILSQFIKSEKDIEEISAKGKNVIV
EEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCEEEE
IGGGDTGTDCVGVSLRQGCKSVVQFEIMDKPPLLRRQNNPWPEWPKVLTVDYAQEEYAEL
EECCCCCCHHHHHHHHHHHHHHHEEEECCCCCHHCCCCCCCCCCCCEEEEECHHHHHHHH
FGKDPRLYNISTKRFVGDAKGNVKEVHTVEVEWKQDKTGKLFPKEIDGTEKVWKADLVLL
HCCCCCEEECCHHHEECCCCCCCEEEEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHH
ALGFLGPEKYLIEQLNVETDKRSNVKTEEGKYSTNVEGIFSAGDMRRGQSLVVWAINEGK
HHHHCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCEEEEEEECCCH
QAAKECNEYLISSNNNNNKQRRLKTL
HHHHHHHHHEECCCCCCCHHHHHCCC
>Mature Secondary Structure 
GDPQGFLKYERKTPEERPSKIRINDWEELYIEMEKDELRMQAARCMNCGTPFCHSGILI
CCCHHHHHHHHCCCCCCCCEEEECCHHHEEEEECHHHHHHHHHHHHCCCCCHHHCCEEH
NNMVSGCPLNNLIPEWNDLVYRNLWKEAYERLIETNSFPEFTGRVCPAPCEKACVNNLVK
HHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHCCCCCCCCHHHHHHHHHHC
DPVSIKSIEYYIIEEAFKNGWVEEDKPSYSTGKKVAVVGSGPAGLSCAWELKRYGHEVTV
CCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHCCCEEEE
FEKYEYPGGLLTYGIPNMKLDKKIVKRRIELMKKSGIQFKTNIDVGKDYPAQKLKEEFDS
EEEECCCCCEEEECCCCCCHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCHHHHHHHHCC
VVLCGGTAKARDLNVEGRNLKGIYFATEFLKSANERILSQFIKSEKDIEEISAKGKNVIV
EEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCEEEE
IGGGDTGTDCVGVSLRQGCKSVVQFEIMDKPPLLRRQNNPWPEWPKVLTVDYAQEEYAEL
EECCCCCCHHHHHHHHHHHHHHHEEEECCCCCHHCCCCCCCCCCCCEEEEECHHHHHHHH
FGKDPRLYNISTKRFVGDAKGNVKEVHTVEVEWKQDKTGKLFPKEIDGTEKVWKADLVLL
HCCCCCEEECCHHHEECCCCCCCEEEEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHH
ALGFLGPEKYLIEQLNVETDKRSNVKTEEGKYSTNVEGIFSAGDMRRGQSLVVWAINEGK
HHHHCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCEEEEEEECCCH
QAAKECNEYLISSNNNNNKQRRLKTL
HHHHHHHHHEECCCCCCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377; 11029411 [H]