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The map label for this gene is amtB [C]

Identifier: 160903227

GI number: 160903227

Start: 1972587

End: 1973912

Strand: Direct

Name: amtB [C]

Synonym: Pmob_1796

Alternate gene names: 160903227

Gene position: 1972587-1973912 (Clockwise)

Preceding gene: 160903226

Following gene: 160903228

Centisome position: 90.92

GC content: 40.95

Gene sequence:

>1326_bases
ATGAAGAGAGTTTGGAAGAGTTTGTTGATGATGTCTGTTGCCTTTATTGTTCCAACCGTTTTACTCGCAGATAGTATTAC
TATGGAGGACGTGGTAAGCTCCATCGATACTATGTGGACTTTACTCGCAGCGTTTTTAGTGTTTTTTATGCAAGCTGGAT
TTGCGCTCGTGGAAGCAGGGTTTACAAGATCAAAGAACACGGTAAACATCATCATGAAAAACTTTACGGATTTTTTAGTT
GGCTCAATACTTTACTGGCTAGTTGGGTTTACTATTATGTTTGGTGTTGGGAATGGTTTCATAGGTTGGCAAGGAAGTTT
TGAATATCTGGGATTAAACATTCCGCTTAACGCTTTTTTAATATTCCAGACGGTATTTGCTGCAACCGCTGCAACTATAG
TATCTGGAGCAATGGCGGAAAGAACTAAATTCACAGGTTATCTGATCTACAGTGTTTTCATCAGTGCATTTATATATCCC
ATAGTTGGTCATTGGATATGGGGTGGAGGTTGGTTATCAGATATGGTTGATTTTGCAGGTTCCACAGTAGTTCATTCCGT
AGGCGGATGGGCTGCACTGATGGGAACAATGGTTTTAGGGCCAAGAACCGGTAAATACAGATCAGATGGAACACCGAATA
AGATTCCAGGACATAGCTTAGTTTTAGCTGCTTTAGGGGTTTTCATCCTATGGTTTGGATGGTTTGGTTTTAACCCTGGA
AGTACCATTTCAGGTTTAAATCTCAAAATCGCTGATATAGCGATGACTACAAATTTAGCTGCCGCATCAGGAGGGTTGGG
GGCGTTGACGATAGGTTGGTTTATTTACAAAAAACCGGACGTCAACTCTACCCTAAATGGTGTTTTAGCTGGATTGGTGG
GAATCACTGCTGGCACAGCAAGTGTTACTAATGTTGGCGCTGCCATAATAGGAGCTTTATCCGGTGGTTTAGTTGTTCTA
GCTATAGATTTTTTCGACAAAATACATATCGATGATCCTGTTGGAGCAATCTCTGTGCATGGAGTTGGTGGAGCGTTCGG
TACATTAATGGTTGGAATATTTGCTGTGGATGGTGGATTACTCTACGGAGGAGGATTAAATCTGTTTTTGACTCAGCTAA
AAGGGGTGCTAGCTGTTGCAGGTTGGACCGTTGTAACTACCTACATCCTTTTTAAAAGTATCGATTTAACAATAGGTTTA
AGGATTGATTCTACTAGTGAAATAGAGGGCTTAGATTTTGCAGAGCATGGATCTGTTAGTTATCCTGATTTAACTCCGCT
TAAAGGGCGAAGCGTGAGAGTAAAAATTGATGAGGAAGGTGAATAA

Upstream 100 bases:

>100_bases
TGCGTCGGTTTTTTATTTTGTAATGGGAGGTGAAAAAAGTCTACGTTAAGTACCATATTCTGGGGTTTGGAAAAAACAAA
AAAAGGAGTGTGAGGGTATT

Downstream 100 bases:

>100_bases
TATGGGATACAAGAAAATTGAATGTTTTATCAGGCCAGAAAAATTTGAGGAAGTCATCGTAGGATTAGAAAAAGTTGGTA
TCGATGGCATTAACATTGAT

Product: ammonium transporter

Products: NH3 [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 441; Mature: 441

Protein sequence:

>441_residues
MKRVWKSLLMMSVAFIVPTVLLADSITMEDVVSSIDTMWTLLAAFLVFFMQAGFALVEAGFTRSKNTVNIIMKNFTDFLV
GSILYWLVGFTIMFGVGNGFIGWQGSFEYLGLNIPLNAFLIFQTVFAATAATIVSGAMAERTKFTGYLIYSVFISAFIYP
IVGHWIWGGGWLSDMVDFAGSTVVHSVGGWAALMGTMVLGPRTGKYRSDGTPNKIPGHSLVLAALGVFILWFGWFGFNPG
STISGLNLKIADIAMTTNLAAASGGLGALTIGWFIYKKPDVNSTLNGVLAGLVGITAGTASVTNVGAAIIGALSGGLVVL
AIDFFDKIHIDDPVGAISVHGVGGAFGTLMVGIFAVDGGLLYGGGLNLFLTQLKGVLAVAGWTVVTTYILFKSIDLTIGL
RIDSTSEIEGLDFAEHGSVSYPDLTPLKGRSVRVKIDEEGE

Sequences:

>Translated_441_residues
MKRVWKSLLMMSVAFIVPTVLLADSITMEDVVSSIDTMWTLLAAFLVFFMQAGFALVEAGFTRSKNTVNIIMKNFTDFLV
GSILYWLVGFTIMFGVGNGFIGWQGSFEYLGLNIPLNAFLIFQTVFAATAATIVSGAMAERTKFTGYLIYSVFISAFIYP
IVGHWIWGGGWLSDMVDFAGSTVVHSVGGWAALMGTMVLGPRTGKYRSDGTPNKIPGHSLVLAALGVFILWFGWFGFNPG
STISGLNLKIADIAMTTNLAAASGGLGALTIGWFIYKKPDVNSTLNGVLAGLVGITAGTASVTNVGAAIIGALSGGLVVL
AIDFFDKIHIDDPVGAISVHGVGGAFGTLMVGIFAVDGGLLYGGGLNLFLTQLKGVLAVAGWTVVTTYILFKSIDLTIGL
RIDSTSEIEGLDFAEHGSVSYPDLTPLKGRSVRVKIDEEGE
>Mature_441_residues
MKRVWKSLLMMSVAFIVPTVLLADSITMEDVVSSIDTMWTLLAAFLVFFMQAGFALVEAGFTRSKNTVNIIMKNFTDFLV
GSILYWLVGFTIMFGVGNGFIGWQGSFEYLGLNIPLNAFLIFQTVFAATAATIVSGAMAERTKFTGYLIYSVFISAFIYP
IVGHWIWGGGWLSDMVDFAGSTVVHSVGGWAALMGTMVLGPRTGKYRSDGTPNKIPGHSLVLAALGVFILWFGWFGFNPG
STISGLNLKIADIAMTTNLAAASGGLGALTIGWFIYKKPDVNSTLNGVLAGLVGITAGTASVTNVGAAIIGALSGGLVVL
AIDFFDKIHIDDPVGAISVHGVGGAFGTLMVGIFAVDGGLLYGGGLNLFLTQLKGVLAVAGWTVVTTYILFKSIDLTIGL
RIDSTSEIEGLDFAEHGSVSYPDLTPLKGRSVRVKIDEEGE

Specific function: Involved In The Uptake Of Ammonia (Probable). [C]

COG id: COG0004

COG function: function code P; Ammonia permease

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ammonium transporter (TC 2.A.49) family [H]

Homologues:

Organism=Escherichia coli, GI1786656, Length=411, Percent_Identity=33.8199513381995, Blast_Score=183, Evalue=2e-47,
Organism=Caenorhabditis elegans, GI32564042, Length=427, Percent_Identity=34.4262295081967, Blast_Score=206, Evalue=2e-53,
Organism=Caenorhabditis elegans, GI17550180, Length=424, Percent_Identity=34.6698113207547, Blast_Score=198, Evalue=5e-51,
Organism=Caenorhabditis elegans, GI17550182, Length=431, Percent_Identity=35.0348027842227, Blast_Score=194, Evalue=6e-50,
Organism=Caenorhabditis elegans, GI133931100, Length=443, Percent_Identity=30.6997742663657, Blast_Score=188, Evalue=6e-48,
Organism=Saccharomyces cerevisiae, GI6324187, Length=434, Percent_Identity=29.7235023041475, Blast_Score=166, Evalue=8e-42,
Organism=Saccharomyces cerevisiae, GI6325396, Length=427, Percent_Identity=26.6978922716628, Blast_Score=131, Evalue=2e-31,
Organism=Saccharomyces cerevisiae, GI6321559, Length=428, Percent_Identity=26.6355140186916, Blast_Score=129, Evalue=9e-31,
Organism=Drosophila melanogaster, GI161078314, Length=409, Percent_Identity=39.119804400978, Blast_Score=227, Evalue=1e-59,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001905
- InterPro:   IPR018047 [H]

Pfam domain/function: PF00909 Ammonium_transp [H]

EC number: NA

Molecular weight: Translated: 46717; Mature: 46717

Theoretical pI: Translated: 5.60; Mature: 5.60

Prosite motif: PS01219 AMMONIUM_TRANSP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CCCCCCCCCCEEEEEECCCCC
>Mature Secondary Structure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CCCCCCCCCCEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NH3 [Periplasm] [C]

Specific reaction: NH3 [Periplasm] = NH3 [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]