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The map label for this gene is eno

Identifier: 160903157

GI number: 160903157

Start: 1885334

End: 1886632

Strand: Direct

Name: eno

Synonym: Pmob_1722

Alternate gene names: 160903157

Gene position: 1885334-1886632 (Clockwise)

Preceding gene: 160903156

Following gene: 160903161

Centisome position: 86.9

GC content: 37.26

Gene sequence:

>1299_bases
ATGGAAAAGTTTTACGATGAAATAGTATCAGTAAAAGCAAGAGAGGTATTGGATTCGAGGGGAAATCCAACCGTTGAAGC
AGAGGTAACTTTATCTACAGGTGTAACTGGTTCAGCTATTGTTCCTTCAGGTGCTTCAACAGGTAAATTTGAGGCTTTAG
AATTAAGAGATGGTAATAAAGATTATTACATGGGAAAAGGTGTTACTAAAGCCGTGAACAACGTAAACAACATTATTGAG
CAAGAGGTTGTTGGATTGAATGCCTTTGATCAAGTAAACGTTGATAGAGTGATGCTGGATTTAGACGGAACAGAAAACAA
AGAAAATCTTGGCGCTAATGCAATATTAGCTGTTTCTATGGCAGCGGCAAGAGCTGCAGCTAACTCCTTGGGACTACCTC
TTTATAAGTATTTAGGAGGCGTGAACGCCAAAGTTTTGCCCGTACCAATGATGAATATAATAAACGGGGGACAACACGCC
GATAATAATCTCGATATTCAAGAGTTCATGATTATGCCTGCTGGATTTAATTCATTTAAAGACGCCTTGAGGGCAGGGGC
TGAAGTCTTCCACAATTTAAAAAATATTTTGAAAAAAGAAGGGCACATAACTTCTGTTGGAGATGAAGGTGGTTTCGCTC
CGAATTTAAATTCCAATGAAGAAGCTATAAAATACATAATTAGAGCCATACAAGCTGCTGGTTACGAACCTGGTAAACAA
ATTTTTATCGCCATGGATGCCGCAGCTTCAGAGTTTTACAATGAAGAAACAAAGAAATACTCTGTTGATGGAAAAGAAAT
GAGTGCTGCTGAATTAGCCGAATATTATATATCGTTGATTGATAAATATCCTATTAAATCATTAGAAGATCCTTTCGATC
AGGATGACTGGGAAGGTTACTCTGAGTTCACCGCAAAAGTGGGAGACAGGGTACAAATAGTTGGTGATGACTTATACGTT
ACTAATGTTAAAAGATTGCAAAAAGGTATAGATTTAAAGGCTACAAACTCTATTTTGATCAAATTGAATCAAATAGGGAG
TGTAACCGAGACTCTAGACGCCATTGAATTAGCCTATAAAAATAATATGACCGCCGTTGTATCTCATCGCTCAGGAGAAA
CGGAAGATTCTTTTATTGCCGATTTGGTAGTAGCTGTAAATGCAGGTTTTATCAAGACTGGCTCACTTTCTAGAACCGAT
AGAATAGCGAAATATAATCAATTGTTAAGAATCGAAGACGAGCTTGGTTCAACTGCACAATATAGAGGCTTGAATGCTTT
TTATTCAATTAAAAAGTAA

Upstream 100 bases:

>100_bases
AGGTACAAAAGTCGATCACTAACAAATTCTTCTTCAAGCATGAGTTCTATTCGAGGTTTAACGAAATATTAAATAATAAA
TTTCTGGAGGTGTTTTTTAG

Downstream 100 bases:

>100_bases
TAAATATTCAATTAACTAAAAAAAACCGGTGATTTATTATTTCACCGGTTTTTTTAATAGATATATTTGTTATAATACTT
GAACGAAATTAATTTTATCA

Product: phosphopyruvate hydratase

Products: NA

Alternate protein names: 2-phospho-D-glycerate hydro-lyase; 2-phosphoglycerate dehydratase

Number of amino acids: Translated: 432; Mature: 432

Protein sequence:

>432_residues
MEKFYDEIVSVKAREVLDSRGNPTVEAEVTLSTGVTGSAIVPSGASTGKFEALELRDGNKDYYMGKGVTKAVNNVNNIIE
QEVVGLNAFDQVNVDRVMLDLDGTENKENLGANAILAVSMAAARAAANSLGLPLYKYLGGVNAKVLPVPMMNIINGGQHA
DNNLDIQEFMIMPAGFNSFKDALRAGAEVFHNLKNILKKEGHITSVGDEGGFAPNLNSNEEAIKYIIRAIQAAGYEPGKQ
IFIAMDAAASEFYNEETKKYSVDGKEMSAAELAEYYISLIDKYPIKSLEDPFDQDDWEGYSEFTAKVGDRVQIVGDDLYV
TNVKRLQKGIDLKATNSILIKLNQIGSVTETLDAIELAYKNNMTAVVSHRSGETEDSFIADLVVAVNAGFIKTGSLSRTD
RIAKYNQLLRIEDELGSTAQYRGLNAFYSIKK

Sequences:

>Translated_432_residues
MEKFYDEIVSVKAREVLDSRGNPTVEAEVTLSTGVTGSAIVPSGASTGKFEALELRDGNKDYYMGKGVTKAVNNVNNIIE
QEVVGLNAFDQVNVDRVMLDLDGTENKENLGANAILAVSMAAARAAANSLGLPLYKYLGGVNAKVLPVPMMNIINGGQHA
DNNLDIQEFMIMPAGFNSFKDALRAGAEVFHNLKNILKKEGHITSVGDEGGFAPNLNSNEEAIKYIIRAIQAAGYEPGKQ
IFIAMDAAASEFYNEETKKYSVDGKEMSAAELAEYYISLIDKYPIKSLEDPFDQDDWEGYSEFTAKVGDRVQIVGDDLYV
TNVKRLQKGIDLKATNSILIKLNQIGSVTETLDAIELAYKNNMTAVVSHRSGETEDSFIADLVVAVNAGFIKTGSLSRTD
RIAKYNQLLRIEDELGSTAQYRGLNAFYSIKK
>Mature_432_residues
MEKFYDEIVSVKAREVLDSRGNPTVEAEVTLSTGVTGSAIVPSGASTGKFEALELRDGNKDYYMGKGVTKAVNNVNNIIE
QEVVGLNAFDQVNVDRVMLDLDGTENKENLGANAILAVSMAAARAAANSLGLPLYKYLGGVNAKVLPVPMMNIINGGQHA
DNNLDIQEFMIMPAGFNSFKDALRAGAEVFHNLKNILKKEGHITSVGDEGGFAPNLNSNEEAIKYIIRAIQAAGYEPGKQ
IFIAMDAAASEFYNEETKKYSVDGKEMSAAELAEYYISLIDKYPIKSLEDPFDQDDWEGYSEFTAKVGDRVQIVGDDLYV
TNVKRLQKGIDLKATNSILIKLNQIGSVTETLDAIELAYKNNMTAVVSHRSGETEDSFIADLVVAVNAGFIKTGSLSRTD
RIAKYNQLLRIEDELGSTAQYRGLNAFYSIKK

Specific function: Catalyzes the reversible conversion of 2- phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis

COG id: COG0148

COG function: function code G; Enolase

Gene ontology:

Cell location: Cytoplasm. Secreted. Cell surface. Note=Fractions of enolase are present in both the cytoplasm and on the cell surface. The export of enolase possibly depends on the covalent binding to the substrate; once secreted, it remains attached to the cell surface

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enolase family

Homologues:

Organism=Homo sapiens, GI4503571, Length=432, Percent_Identity=52.5462962962963, Blast_Score=437, Evalue=1e-123,
Organism=Homo sapiens, GI5803011, Length=432, Percent_Identity=53.0092592592593, Blast_Score=435, Evalue=1e-122,
Organism=Homo sapiens, GI301897477, Length=425, Percent_Identity=53.6470588235294, Blast_Score=427, Evalue=1e-119,
Organism=Homo sapiens, GI301897469, Length=425, Percent_Identity=53.6470588235294, Blast_Score=427, Evalue=1e-119,
Organism=Homo sapiens, GI301897479, Length=423, Percent_Identity=48.936170212766, Blast_Score=370, Evalue=1e-102,
Organism=Homo sapiens, GI169201331, Length=335, Percent_Identity=28.0597014925373, Blast_Score=117, Evalue=2e-26,
Organism=Homo sapiens, GI169201757, Length=335, Percent_Identity=28.0597014925373, Blast_Score=117, Evalue=2e-26,
Organism=Homo sapiens, GI239744207, Length=335, Percent_Identity=28.0597014925373, Blast_Score=117, Evalue=2e-26,
Organism=Escherichia coli, GI1789141, Length=424, Percent_Identity=57.5471698113208, Blast_Score=474, Evalue=1e-135,
Organism=Caenorhabditis elegans, GI71995829, Length=433, Percent_Identity=52.4249422632794, Blast_Score=423, Evalue=1e-119,
Organism=Caenorhabditis elegans, GI17536383, Length=433, Percent_Identity=52.4249422632794, Blast_Score=423, Evalue=1e-118,
Organism=Caenorhabditis elegans, GI32563855, Length=194, Percent_Identity=52.0618556701031, Blast_Score=197, Evalue=9e-51,
Organism=Saccharomyces cerevisiae, GI6323985, Length=434, Percent_Identity=50.9216589861751, Blast_Score=408, Evalue=1e-115,
Organism=Saccharomyces cerevisiae, GI6324974, Length=434, Percent_Identity=50.9216589861751, Blast_Score=407, Evalue=1e-114,
Organism=Saccharomyces cerevisiae, GI6324969, Length=434, Percent_Identity=50.9216589861751, Blast_Score=407, Evalue=1e-114,
Organism=Saccharomyces cerevisiae, GI6321693, Length=441, Percent_Identity=50.1133786848073, Blast_Score=394, Evalue=1e-110,
Organism=Saccharomyces cerevisiae, GI6321968, Length=441, Percent_Identity=51.2471655328798, Blast_Score=377, Evalue=1e-105,
Organism=Drosophila melanogaster, GI24580918, Length=431, Percent_Identity=51.9721577726218, Blast_Score=404, Evalue=1e-113,
Organism=Drosophila melanogaster, GI24580916, Length=431, Percent_Identity=51.9721577726218, Blast_Score=404, Evalue=1e-113,
Organism=Drosophila melanogaster, GI24580920, Length=431, Percent_Identity=51.9721577726218, Blast_Score=404, Evalue=1e-113,
Organism=Drosophila melanogaster, GI24580914, Length=431, Percent_Identity=51.9721577726218, Blast_Score=404, Evalue=1e-113,
Organism=Drosophila melanogaster, GI281360527, Length=431, Percent_Identity=51.9721577726218, Blast_Score=402, Evalue=1e-112,
Organism=Drosophila melanogaster, GI17137654, Length=431, Percent_Identity=51.9721577726218, Blast_Score=402, Evalue=1e-112,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): ENO_PETMO (A9BIS7)

Other databases:

- EMBL:   CP000879
- RefSeq:   YP_001568738.1
- ProteinModelPortal:   A9BIS7
- SMR:   A9BIS7
- GeneID:   5757499
- GenomeReviews:   CP000879_GR
- KEGG:   pmo:Pmob_1722
- HOGENOM:   HBG726599
- OMA:   DIAVGTN
- ProtClustDB:   PRK00077
- BioCyc:   PMOB403833:PMOB_1722-MONOMER
- GO:   GO:0006096
- HAMAP:   MF_00318
- InterPro:   IPR000941
- InterPro:   IPR020810
- InterPro:   IPR020809
- InterPro:   IPR020811
- PIRSF:   PIRSF001400
- PRINTS:   PR00148
- TIGRFAMs:   TIGR01060

Pfam domain/function: PF00113 Enolase_C; PF03952 Enolase_N

EC number: =4.2.1.11

Molecular weight: Translated: 47137; Mature: 47137

Theoretical pI: Translated: 4.58; Mature: 4.58

Prosite motif: PS00164 ENOLASE

Important sites: ACT_SITE 209-209 ACT_SITE 341-341 BINDING 159-159 BINDING 168-168 BINDING 289-289 BINDING 316-316 BINDING 341-341 BINDING 392-392

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKFYDEIVSVKAREVLDSRGNPTVEAEVTLSTGVTGSAIVPSGASTGKFEALELRDGNK
CCHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCEECCCCCCCCCEEEEEEECCCC
DYYMGKGVTKAVNNVNNIIEQEVVGLNAFDQVNVDRVMLDLDGTENKENLGANAILAVSM
CEEECCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCCHHCCCCHHHHHHH
AAARAAANSLGLPLYKYLGGVNAKVLPVPMMNIINGGQHADNNLDIQEFMIMPAGFNSFK
HHHHHHHHHCCCHHHHHHCCCCCEEECCCHHHHHCCCCCCCCCCCHHHHEEECCCCHHHH
DALRAGAEVFHNLKNILKKEGHITSVGDEGGFAPNLNSNEEAIKYIIRAIQAAGYEPGKQ
HHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCE
IFIAMDAAASEFYNEETKKYSVDGKEMSAAELAEYYISLIDKYPIKSLEDPFDQDDWEGY
EEEEEEHHHHHHHHHHHHEEECCCCHHHHHHHHHHHHHHHHHCCCHHCCCCCCCCCCCCH
SEFTAKVGDRVQIVGDDLYVTNVKRLQKGIDLKATNSILIKLNQIGSVTETLDAIELAYK
HHHHHHCCCEEEEEECCEEEHHHHHHHHCCCEEECCEEEEEEECCCCHHHHHHHHHHHHC
NNMTAVVSHRSGETEDSFIADLVVAVNAGFIKTGSLSRTDRIAKYNQLLRIEDELGSTAQ
CCCEEEEECCCCCCCHHHHHHHHHHHCCCEEECCCCCHHHHHHHHHHHEEEHHHHCCCHH
YRGLNAFYSIKK
HCCCCCEEECCC
>Mature Secondary Structure
MEKFYDEIVSVKAREVLDSRGNPTVEAEVTLSTGVTGSAIVPSGASTGKFEALELRDGNK
CCHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCEECCCCCCCCCEEEEEEECCCC
DYYMGKGVTKAVNNVNNIIEQEVVGLNAFDQVNVDRVMLDLDGTENKENLGANAILAVSM
CEEECCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCCHHCCCCHHHHHHH
AAARAAANSLGLPLYKYLGGVNAKVLPVPMMNIINGGQHADNNLDIQEFMIMPAGFNSFK
HHHHHHHHHCCCHHHHHHCCCCCEEECCCHHHHHCCCCCCCCCCCHHHHEEECCCCHHHH
DALRAGAEVFHNLKNILKKEGHITSVGDEGGFAPNLNSNEEAIKYIIRAIQAAGYEPGKQ
HHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCE
IFIAMDAAASEFYNEETKKYSVDGKEMSAAELAEYYISLIDKYPIKSLEDPFDQDDWEGY
EEEEEEHHHHHHHHHHHHEEECCCCHHHHHHHHHHHHHHHHHCCCHHCCCCCCCCCCCCH
SEFTAKVGDRVQIVGDDLYVTNVKRLQKGIDLKATNSILIKLNQIGSVTETLDAIELAYK
HHHHHHCCCEEEEEECCEEEHHHHHHHHCCCEEECCEEEEEEECCCCHHHHHHHHHHHHC
NNMTAVVSHRSGETEDSFIADLVVAVNAGFIKTGSLSRTDRIAKYNQLLRIEDELGSTAQ
CCCEEEEECCCCCCCHHHHHHHHHHHCCCEEECCCCCHHHHHHHHHHHEEEHHHHCCCHH
YRGLNAFYSIKK
HCCCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA