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The map label for this gene is purR [H]

Identifier: 160903042

GI number: 160903042

Start: 1749465

End: 1750391

Strand: Direct

Name: purR [H]

Synonym: Pmob_1606

Alternate gene names: 160903042

Gene position: 1749465-1750391 (Clockwise)

Preceding gene: 160903041

Following gene: 160903043

Centisome position: 80.64

GC content: 30.85

Gene sequence:

>927_bases
ATGCGAAAACCAACGATAAAAGACATTCCAAAGTCTTTGAATCTCTCTCCTTCTACAGGCATATTTAAAAACTCAAAATC
AAAGATCATCGGTGCTATTGCGTTTGATATAAGTAATCCTTTCTTTTTGGATTTTTTAAAGGGTGTAGAAGATGTTTTAT
TTCCACGTGAGTATAAGATTTTGTTAAGTAACGTCGACGAGAATATAGATAAAGAAAAGACCTATTTAAGCTGGATGATA
GGTCATGGGGTCGAAGGTATTTTAGCTTCACCAGTTAGAGAAAGAAACGGTAAAAGTAATTTGAGATTATATCAAAATTT
TTACAAAATGGGCATTCCAATAGTTTTCTACGATAGGATGTTTTATAGTCAAGGGCAATTTGATCATGTTATTGTAGACA
ACCAAGATGCAATCATTCAGGCCTTGATGCATCTAAAAGAAACGGGTCATGAAAAGATAGGTATTTTTTTGTCTAAAAAA
GGAATATTCACTATAGAAGAAAGACTCAAAGGGTTTCTAAAGGGTTGTAAACTTTTAAATATTCCCGTTAAAAATGAATG
GATCTGTGAAGATATATACCCTCATGAAAAAGCTATAGAAGTTTTAAATTGTCTAAAGAGAAAGAATACTTTACCAACGG
CGATAATAGCAACAAACAACAATTTAACTAAGTATATAGTTTACGCTGCTAAAAAAAATAATATAAAAATTCCTGAAGAA
CTGTCTTTAGTTGGATTTGATGATTCACTTGAAAATGAATTGATAGATCCGCCTTTAACGACTATAAAACAACCAATATT
AGAAGAAGGTAAGATAGCTGCCACATCTTTACTATCAAGAATAGAAGACAGATATGCCAGACCTATGAATGTAACATTAA
AAAGTCAATTAATAAAAAGGAAATCAGTAAAGAATTTGAATTTATAA

Upstream 100 bases:

>100_bases
TAATCACTGCTGTATTTTTCACACCTACTCTTTGAATAATCTGAAAAATATACAAGAATTAAAAAAACCAGTCATTGAAA
TATTCTCAGGAGGTAATCCC

Downstream 100 bases:

>100_bases
TAGATTAAGTCATATAAGATATTTTGACAATCTTCTTAACTTAATAATAAATTTCAATTCATATACTCCTATATTAATTA
TATCTTCTGTCCCGCAAAAC

Product: periplasmic binding protein/LacI transcriptional regulator

Products: NA

Alternate protein names: Pur regulon repressor; Purine nucleotide synthesis repressor [H]

Number of amino acids: Translated: 308; Mature: 308

Protein sequence:

>308_residues
MRKPTIKDIPKSLNLSPSTGIFKNSKSKIIGAIAFDISNPFFLDFLKGVEDVLFPREYKILLSNVDENIDKEKTYLSWMI
GHGVEGILASPVRERNGKSNLRLYQNFYKMGIPIVFYDRMFYSQGQFDHVIVDNQDAIIQALMHLKETGHEKIGIFLSKK
GIFTIEERLKGFLKGCKLLNIPVKNEWICEDIYPHEKAIEVLNCLKRKNTLPTAIIATNNNLTKYIVYAAKKNNIKIPEE
LSLVGFDDSLENELIDPPLTTIKQPILEEGKIAATSLLSRIEDRYARPMNVTLKSQLIKRKSVKNLNL

Sequences:

>Translated_308_residues
MRKPTIKDIPKSLNLSPSTGIFKNSKSKIIGAIAFDISNPFFLDFLKGVEDVLFPREYKILLSNVDENIDKEKTYLSWMI
GHGVEGILASPVRERNGKSNLRLYQNFYKMGIPIVFYDRMFYSQGQFDHVIVDNQDAIIQALMHLKETGHEKIGIFLSKK
GIFTIEERLKGFLKGCKLLNIPVKNEWICEDIYPHEKAIEVLNCLKRKNTLPTAIIATNNNLTKYIVYAAKKNNIKIPEE
LSLVGFDDSLENELIDPPLTTIKQPILEEGKIAATSLLSRIEDRYARPMNVTLKSQLIKRKSVKNLNL
>Mature_308_residues
MRKPTIKDIPKSLNLSPSTGIFKNSKSKIIGAIAFDISNPFFLDFLKGVEDVLFPREYKILLSNVDENIDKEKTYLSWMI
GHGVEGILASPVRERNGKSNLRLYQNFYKMGIPIVFYDRMFYSQGQFDHVIVDNQDAIIQALMHLKETGHEKIGIFLSKK
GIFTIEERLKGFLKGCKLLNIPVKNEWICEDIYPHEKAIEVLNCLKRKNTLPTAIIATNNNLTKYIVYAAKKNNIKIPEE
LSLVGFDDSLENELIDPPLTTIKQPILEEGKIAATSLLSRIEDRYARPMNVTLKSQLIKRKSVKNLNL

Specific function: Is the main repressor of the genes involved in the de novo synthesis of purine nucleotides, regulating purB, purC, purEK, purF, purHD, purL, purMN and guaBA expression. PurR is allosterically activated to bind its cognate DNA by binding the purine corepre

COG id: COG1609

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787948, Length=298, Percent_Identity=27.1812080536913, Blast_Score=114, Evalue=6e-27,
Organism=Escherichia coli, GI1790369, Length=293, Percent_Identity=25.938566552901, Blast_Score=114, Evalue=1e-26,
Organism=Escherichia coli, GI1787580, Length=268, Percent_Identity=25.7462686567164, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI1787906, Length=289, Percent_Identity=22.8373702422145, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI1788474, Length=254, Percent_Identity=28.740157480315, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI1789202, Length=305, Percent_Identity=24.2622950819672, Blast_Score=81, Evalue=9e-17,
Organism=Escherichia coli, GI1790194, Length=305, Percent_Identity=23.6065573770492, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI48994940, Length=269, Percent_Identity=22.6765799256506, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1790715, Length=284, Percent_Identity=22.5352112676056, Blast_Score=68, Evalue=7e-13,
Organism=Escherichia coli, GI1786540, Length=195, Percent_Identity=24.6153846153846, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 35186; Mature: 35186

Theoretical pI: Translated: 9.70; Mature: 9.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKPTIKDIPKSLNLSPSTGIFKNSKSKIIGAIAFDISNPFFLDFLKGVEDVLFPREYKI
CCCCCHHHCCHHCCCCCCCCCCCCCCCCEEEEEEEECCCHHHHHHHHHHHHHHCCHHHHH
LLSNVDENIDKEKTYLSWMIGHGVEGILASPVRERNGKSNLRLYQNFYKMGIPIVFYDRM
HHHHHCCCCHHHHHHHHHHHHCCCCHHHHCCHHHCCCCCHHHHHHHHHHHCCCEEEEHHH
FYSQGQFDHVIVDNQDAIIQALMHLKETGHEKIGIFLSKKGIFTIEERLKGFLKGCKLLN
HHCCCCCCEEEECCHHHHHHHHHHHHHCCCCEEEEEEECCCCEEHHHHHHHHHHCCEEEE
IPVKNEWICEDIYPHEKAIEVLNCLKRKNTLPTAIIATNNNLTKYIVYAAKKNNIKIPEE
CCCCCCEECCCCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCEEEEEEEECCCCCCCCCC
LSLVGFDDSLENELIDPPLTTIKQPILEEGKIAATSLLSRIEDRYARPMNVTLKSQLIKR
CEEEECCCHHHHHCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHH
KSVKNLNL
HHHCCCCC
>Mature Secondary Structure
MRKPTIKDIPKSLNLSPSTGIFKNSKSKIIGAIAFDISNPFFLDFLKGVEDVLFPREYKI
CCCCCHHHCCHHCCCCCCCCCCCCCCCCEEEEEEEECCCHHHHHHHHHHHHHHCCHHHHH
LLSNVDENIDKEKTYLSWMIGHGVEGILASPVRERNGKSNLRLYQNFYKMGIPIVFYDRM
HHHHHCCCCHHHHHHHHHHHHCCCCHHHHCCHHHCCCCCHHHHHHHHHHHCCCEEEEHHH
FYSQGQFDHVIVDNQDAIIQALMHLKETGHEKIGIFLSKKGIFTIEERLKGFLKGCKLLN
HHCCCCCCEEEECCHHHHHHHHHHHHHCCCCEEEEEEECCCCEEHHHHHHHHHHCCEEEE
IPVKNEWICEDIYPHEKAIEVLNCLKRKNTLPTAIIATNNNLTKYIVYAAKKNNIKIPEE
CCCCCCEECCCCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCEEEEEEEECCCCCCCCCC
LSLVGFDDSLENELIDPPLTTIKQPILEEGKIAATSLLSRIEDRYARPMNVTLKSQLIKR
CEEEECCCHHHHHCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHH
KSVKNLNL
HHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA