Click here to switch to the map view.

The map label for this gene is gdhA [H]

Identifier: 160903021

GI number: 160903021

Start: 1724519

End: 1725814

Strand: Direct

Name: gdhA [H]

Synonym: Pmob_1583

Alternate gene names: 160903021

Gene position: 1724519-1725814 (Clockwise)

Preceding gene: 160903017

Following gene: 160903033

Centisome position: 79.49

GC content: 38.5

Gene sequence:

>1296_bases
ATGGAAGAAACAAAAAAGGTAAGCTTGTACGATAACGCCGTTAAGCAATTTGACAGAGCCGCTAGAATTATGGAACTAGA
TAGAAATTTAAGAGAAGTATTAATCAAGCCAAAAAGAGAACTTACTGTTAATTTCCCAGTACGCATGGATGATGGGTCAA
TCAAAGTTTTTACAGGTTATAGGGTACAACACAATGTTTCTAGAGGTCCGGCAAAAGGTGGAATTAGGTACCATCCAAAT
GTTACATTAGATGAAGTTAAAGCATTGGCTTTTTGGATGACTTGGAAGTCAGCAGTGGTGGATATCCCCTATGGTGGGGC
TAAAGGCGGAGTTACAGTGAATCCGTTTAAACTTTCTGATTCTGAATTAGAGAGATTATCACGAAGATTTTTCTCTGAAA
TTCAAATCATAATAGGTGAGGAAAAAGATATCCCTGCTCCAGATGTAAACACTGATGGTCAAATAATGGCTTGGTGGATG
GATACATATTCGATGAATATTGGTCATACTACCTTAGGCATAGTCACGGGGAAGCCTTTAGAGATCGGCGGATCTGAAGG
AAGAACAGAGGCCACTGGAAGAGGAGTAAATATATGTATTGAGGAAGCTGTAAAGTATTTGAGAGACAAAGGGAAGCTAA
ATAAGAAAGATGAAGCAATAACCGTTGCCATTCAAGGGTTTGGTAATGTTGGTTCTTACTTAGCTTTGACCCTTACAGAA
GAAACTAAATATAGGTTGGTTGCAATATCTGATTATAGTGGTGGTTTTTATAAAGAGTCCGGTTTCACAGCAGAAGAGAT
TCGTAGTCTAATGGATAGAACTAAAGGTAGAAAAGCATTGTTATTGGATGTTAACGAAGAAGGTTATAAAGAGATAACGA
ACGAAGAATTATTGAAGCTAGATGTTGATGTTTTGGCCCCTTGTGCTTTAGAAAATGCAGTAAATGAAGATAATGCAGAG
GAAATAAGAGCAAAACTCATTGTCGAAGGAGCTAATGGACCATTAACTCCCGAAGCGGACGAGATATTATTGTCTAAAAA
TGTCTTCATAGTACCGGATTTCTTGGCAAATGCTGGTGGAGTAACGGTTTCGTACTTTGAGTGGGTGCAAGGGCTTCAGT
GGAATTTTTGGGAACTAGAAGACATTAGAAAAGCCCTTCATAAAAAGATGAAGAATGCATTTTGTGATGTTGCTCAAACG
ATGGGAAAGTATGAAGTTGATATGAGAACAGCTGCTTATGTGAGAGCCATTGAACGTGTGGCAAATGCTACAAAGTTAAG
AGGAATCTATCCCTGA

Upstream 100 bases:

>100_bases
ACTTTGAGAAAAAAGGGATAAAATATTTATGTACAATAAAAGTGAAGTTCTATATTTTAATAGAAAGAGAGAAACAATTA
GAAAAAGGAGGATTACAAAG

Downstream 100 bases:

>100_bases
TAAGAAATAACAAAATATACCCCTTCCTTAATTATTTAGGAAGGGGTAATTTTTTGGTTACTTTGTTTAAGTAAAATAGA
ACGAGTTTATTTAATAAAGG

Product: Glu/Leu/Phe/Val dehydrogenase

Products: NA

Alternate protein names: GDH [H]

Number of amino acids: Translated: 431; Mature: 431

Protein sequence:

>431_residues
MEETKKVSLYDNAVKQFDRAARIMELDRNLREVLIKPKRELTVNFPVRMDDGSIKVFTGYRVQHNVSRGPAKGGIRYHPN
VTLDEVKALAFWMTWKSAVVDIPYGGAKGGVTVNPFKLSDSELERLSRRFFSEIQIIIGEEKDIPAPDVNTDGQIMAWWM
DTYSMNIGHTTLGIVTGKPLEIGGSEGRTEATGRGVNICIEEAVKYLRDKGKLNKKDEAITVAIQGFGNVGSYLALTLTE
ETKYRLVAISDYSGGFYKESGFTAEEIRSLMDRTKGRKALLLDVNEEGYKEITNEELLKLDVDVLAPCALENAVNEDNAE
EIRAKLIVEGANGPLTPEADEILLSKNVFIVPDFLANAGGVTVSYFEWVQGLQWNFWELEDIRKALHKKMKNAFCDVAQT
MGKYEVDMRTAAYVRAIERVANATKLRGIYP

Sequences:

>Translated_431_residues
MEETKKVSLYDNAVKQFDRAARIMELDRNLREVLIKPKRELTVNFPVRMDDGSIKVFTGYRVQHNVSRGPAKGGIRYHPN
VTLDEVKALAFWMTWKSAVVDIPYGGAKGGVTVNPFKLSDSELERLSRRFFSEIQIIIGEEKDIPAPDVNTDGQIMAWWM
DTYSMNIGHTTLGIVTGKPLEIGGSEGRTEATGRGVNICIEEAVKYLRDKGKLNKKDEAITVAIQGFGNVGSYLALTLTE
ETKYRLVAISDYSGGFYKESGFTAEEIRSLMDRTKGRKALLLDVNEEGYKEITNEELLKLDVDVLAPCALENAVNEDNAE
EIRAKLIVEGANGPLTPEADEILLSKNVFIVPDFLANAGGVTVSYFEWVQGLQWNFWELEDIRKALHKKMKNAFCDVAQT
MGKYEVDMRTAAYVRAIERVANATKLRGIYP
>Mature_431_residues
MEETKKVSLYDNAVKQFDRAARIMELDRNLREVLIKPKRELTVNFPVRMDDGSIKVFTGYRVQHNVSRGPAKGGIRYHPN
VTLDEVKALAFWMTWKSAVVDIPYGGAKGGVTVNPFKLSDSELERLSRRFFSEIQIIIGEEKDIPAPDVNTDGQIMAWWM
DTYSMNIGHTTLGIVTGKPLEIGGSEGRTEATGRGVNICIEEAVKYLRDKGKLNKKDEAITVAIQGFGNVGSYLALTLTE
ETKYRLVAISDYSGGFYKESGFTAEEIRSLMDRTKGRKALLLDVNEEGYKEITNEELLKLDVDVLAPCALENAVNEDNAE
EIRAKLIVEGANGPLTPEADEILLSKNVFIVPDFLANAGGVTVSYFEWVQGLQWNFWELEDIRKALHKKMKNAFCDVAQT
MGKYEVDMRTAAYVRAIERVANATKLRGIYP

Specific function: Unknown

COG id: COG0334

COG function: function code E; Glutamate dehydrogenase/leucine dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Glu/Leu/Phe/Val dehydrogenases family [H]

Homologues:

Organism=Homo sapiens, GI31377775, Length=484, Percent_Identity=36.5702479338843, Blast_Score=275, Evalue=4e-74,
Organism=Homo sapiens, GI4885281, Length=484, Percent_Identity=36.5702479338843, Blast_Score=271, Evalue=6e-73,
Organism=Escherichia coli, GI1788059, Length=409, Percent_Identity=32.518337408313, Blast_Score=185, Evalue=4e-48,
Organism=Caenorhabditis elegans, GI17544676, Length=454, Percent_Identity=35.0220264317181, Blast_Score=246, Evalue=2e-65,
Organism=Saccharomyces cerevisiae, GI6319256, Length=396, Percent_Identity=32.8282828282828, Blast_Score=163, Evalue=5e-41,
Organism=Saccharomyces cerevisiae, GI6324951, Length=402, Percent_Identity=31.592039800995, Blast_Score=156, Evalue=6e-39,
Organism=Drosophila melanogaster, GI45553475, Length=448, Percent_Identity=33.9285714285714, Blast_Score=252, Evalue=4e-67,
Organism=Drosophila melanogaster, GI24649283, Length=373, Percent_Identity=39.9463806970509, Blast_Score=251, Evalue=7e-67,
Organism=Drosophila melanogaster, GI45549226, Length=348, Percent_Identity=38.7931034482759, Blast_Score=247, Evalue=9e-66,
Organism=Drosophila melanogaster, GI24649460, Length=288, Percent_Identity=37.8472222222222, Blast_Score=194, Evalue=1e-49,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 9362 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006095
- InterPro:   IPR006096
- InterPro:   IPR006097
- InterPro:   IPR014362
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00208 ELFV_dehydrog; PF02812 ELFV_dehydrog_N [H]

EC number: =1.4.1.3 [H]

Molecular weight: Translated: 48297; Mature: 48297

Theoretical pI: Translated: 5.31; Mature: 5.31

Prosite motif: PS00074 GLFV_DEHYDROGENASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEETKKVSLYDNAVKQFDRAARIMELDRNLREVLIKPKRELTVNFPVRMDDGSIKVFTGY
CCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCEEECCCCEEEEEEE
RVQHNVSRGPAKGGIRYHPNVTLDEVKALAFWMTWKSAVVDIPYGGAKGGVTVNPFKLSD
EEEECCCCCCCCCCCEECCCCCHHHHHHHHHHEEHHHEEEECCCCCCCCCEEECCEECCH
SELERLSRRFFSEIQIIIGEEKDIPAPDVNTDGQIMAWWMDTYSMNIGHTTLGIVTGKPL
HHHHHHHHHHHHHEEEEEECCCCCCCCCCCCCCCEEEEEEEEEEECCCCEEEEEEECCCE
EIGGSEGRTEATGRGVNICIEEAVKYLRDKGKLNKKDEAITVAIQGFGNVGSYLALTLTE
EECCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCCCCEEEEEEEC
ETKYRLVAISDYSGGFYKESGFTAEEIRSLMDRTKGRKALLLDVNEEGYKEITNEELLKL
CCCEEEEEEEECCCCEEECCCCCHHHHHHHHHHCCCCEEEEEECCCHHHHHHCCHHHHEE
DVDVLAPCALENAVNEDNAEEIRAKLIVEGANGPLTPEADEILLSKNVFIVPDFLANAGG
CHHHHCHHHHHHHCCCCCHHHHEEEEEEECCCCCCCCCHHHEEEECCEEEEEHHHCCCCC
VTVSYFEWVQGLQWNFWELEDIRKALHKKMKNAFCDVAQTMGKYEVDMRTAAYVRAIERV
CEEHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHH
ANATKLRGIYP
HHHHHCCCCCC
>Mature Secondary Structure
MEETKKVSLYDNAVKQFDRAARIMELDRNLREVLIKPKRELTVNFPVRMDDGSIKVFTGY
CCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCEEECCCCEEEEEEE
RVQHNVSRGPAKGGIRYHPNVTLDEVKALAFWMTWKSAVVDIPYGGAKGGVTVNPFKLSD
EEEECCCCCCCCCCCEECCCCCHHHHHHHHHHEEHHHEEEECCCCCCCCCEEECCEECCH
SELERLSRRFFSEIQIIIGEEKDIPAPDVNTDGQIMAWWMDTYSMNIGHTTLGIVTGKPL
HHHHHHHHHHHHHEEEEEECCCCCCCCCCCCCCCEEEEEEEEEEECCCCEEEEEEECCCE
EIGGSEGRTEATGRGVNICIEEAVKYLRDKGKLNKKDEAITVAIQGFGNVGSYLALTLTE
EECCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCCCCEEEEEEEC
ETKYRLVAISDYSGGFYKESGFTAEEIRSLMDRTKGRKALLLDVNEEGYKEITNEELLKL
CCCEEEEEEEECCCCEEECCCCCHHHHHHHHHHCCCCEEEEEECCCHHHHHHCCHHHHEE
DVDVLAPCALENAVNEDNAEEIRAKLIVEGANGPLTPEADEILLSKNVFIVPDFLANAGG
CHHHHCHHHHHHHCCCCCHHHHEEEEEEECCCCCCCCCHHHEEEECCEEEEEHHHCCCCC
VTVSYFEWVQGLQWNFWELEDIRKALHKKMKNAFCDVAQTMGKYEVDMRTAAYVRAIERV
CEEHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHH
ANATKLRGIYP
HHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9680336; 10360571; 9135121; 9654452; 10366510 [H]