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The map label for this gene is buk1 [H]

Identifier: 160902915

GI number: 160902915

Start: 1584980

End: 1586068

Strand: Direct

Name: buk1 [H]

Synonym: Pmob_1470

Alternate gene names: 160902915

Gene position: 1584980-1586068 (Clockwise)

Preceding gene: 160902914

Following gene: 160902916

Centisome position: 73.06

GC content: 38.02

Gene sequence:

>1089_bases
ATGTATAAAATATTGGTCATAAATCCAGGTGCAACGTCTACAAAGCTCTCACTTTTTGAGGATGAGAAAGAAGTAATCTC
AGAAAGTATAGAGCATTCCGTTAGCGAGATAGAAAATTGTAAAACGATTATGGACCAACTTCCTTTTCGTAAAAAGTGCG
TAGAAGATTTTCTAAAAAGATACAACATATCTATCAATGAATTAGATGCGATAGCTGCTAGAGGGGGGATACTTCCTCCT
ATGAAAAGTGGAACTTACCAGGTGAATGAGGATATGGTTAATTACCTAAAAACCAAAACAAGAGTTGAACACGCATCGAA
CCTAGCTGCTGTAATAGCCTTTGAACTATCAAAGAATTCCTCGAAAGGCTTACCTGTTTTCATTACTGATCCTATTTCTG
TTGATGAGTTTATCCCCGAGGCGAGAATTTCTGGAATACCACAACTTGAAAGACACAGTTTATTCCACGCTTTGAACATG
AAAATAGTTGCAAGATTCGCTGCTAAAGAATTGAATAAAGAATATGAAAAATGCAATTTTGTCATAGCTCACTTGGGCGG
TGGAATCTCTGTTGGTGCGCAGAGAAAAGGCCTTATGATCGACGTTAACAACGCAACAGATGAAGGACCTTTTAGCTCAC
AAAGAACGGGAGAATTACCAATGGGGGACCTTGCAAAATGGATTTTTAAAAATATGCATTCATACTCCAAAAATGATGTC
AAATCAACATTTGTAGGAAAAGGAGGTTTGTTTGCATATTTAAAAACAGCAAGTTTAAAAGATGCTTTAAAACTTGCCGA
AAAAGATGAATATGCAAAATTAATTGTAGAAGCCATGGCTTATCAAGTCTCAAAGGAAATAGGTGGGATGGCAGCAATTC
TGGGTGGAGATCTCGATGCAATTATCTTAACTGGTGGAATGGCTCACAGTGACTACTTTGTTGAATTGATCAAAAGAAGG
ATCGATAAGTTAGGGGTGGTAATGGTTTACCCTGGAGCTTACGAGATGGAAGGACTCGCCTTAGGAGCCTTGAGAGCACT
CGAACAGTTGGAAGATACAAAGATATGGGAGGGTGAAAATATTTTATGA

Upstream 100 bases:

>100_bases
AAAAGAGGGTATAAGAAGATTGAGAAAAGTTGCAGAAAATTATGTAAATGCGAATTCTATCCGTAGCAAATCCTAAAAAA
CTGATTATGGGAGGAGCTTT

Downstream 100 bases:

>100_bases
AAATTGAGAACATTAGACAGATTATCGAATTAGTAAAAAATGAGCACAGATCAAAAACGATTGCGGTGGCAGCTGCCGAA
GATGATGTTGTCTTGAAAGC

Product: butyrate kinase

Products: NA

Alternate protein names: BK 1; Branched-chain carboxylic acid kinase 1 [H]

Number of amino acids: Translated: 362; Mature: 362

Protein sequence:

>362_residues
MYKILVINPGATSTKLSLFEDEKEVISESIEHSVSEIENCKTIMDQLPFRKKCVEDFLKRYNISINELDAIAARGGILPP
MKSGTYQVNEDMVNYLKTKTRVEHASNLAAVIAFELSKNSSKGLPVFITDPISVDEFIPEARISGIPQLERHSLFHALNM
KIVARFAAKELNKEYEKCNFVIAHLGGGISVGAQRKGLMIDVNNATDEGPFSSQRTGELPMGDLAKWIFKNMHSYSKNDV
KSTFVGKGGLFAYLKTASLKDALKLAEKDEYAKLIVEAMAYQVSKEIGGMAAILGGDLDAIILTGGMAHSDYFVELIKRR
IDKLGVVMVYPGAYEMEGLALGALRALEQLEDTKIWEGENIL

Sequences:

>Translated_362_residues
MYKILVINPGATSTKLSLFEDEKEVISESIEHSVSEIENCKTIMDQLPFRKKCVEDFLKRYNISINELDAIAARGGILPP
MKSGTYQVNEDMVNYLKTKTRVEHASNLAAVIAFELSKNSSKGLPVFITDPISVDEFIPEARISGIPQLERHSLFHALNM
KIVARFAAKELNKEYEKCNFVIAHLGGGISVGAQRKGLMIDVNNATDEGPFSSQRTGELPMGDLAKWIFKNMHSYSKNDV
KSTFVGKGGLFAYLKTASLKDALKLAEKDEYAKLIVEAMAYQVSKEIGGMAAILGGDLDAIILTGGMAHSDYFVELIKRR
IDKLGVVMVYPGAYEMEGLALGALRALEQLEDTKIWEGENIL
>Mature_362_residues
MYKILVINPGATSTKLSLFEDEKEVISESIEHSVSEIENCKTIMDQLPFRKKCVEDFLKRYNISINELDAIAARGGILPP
MKSGTYQVNEDMVNYLKTKTRVEHASNLAAVIAFELSKNSSKGLPVFITDPISVDEFIPEARISGIPQLERHSLFHALNM
KIVARFAAKELNKEYEKCNFVIAHLGGGISVGAQRKGLMIDVNNATDEGPFSSQRTGELPMGDLAKWIFKNMHSYSKNDV
KSTFVGKGGLFAYLKTASLKDALKLAEKDEYAKLIVEAMAYQVSKEIGGMAAILGGDLDAIILTGGMAHSDYFVELIKRR
IDKLGVVMVYPGAYEMEGLALGALRALEQLEDTKIWEGENIL

Specific function: Anaerobic degradation of L-threonine to propionate. [C]

COG id: COG3426

COG function: function code C; Butyrate kinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the acetokinase family [H]

Homologues:

Organism=Escherichia coli, GI145693184, Length=351, Percent_Identity=24.2165242165242, Blast_Score=66, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000890
- InterPro:   IPR011245 [H]

Pfam domain/function: PF00871 Acetate_kinase [H]

EC number: =2.7.2.7 [H]

Molecular weight: Translated: 40023; Mature: 40023

Theoretical pI: Translated: 5.96; Mature: 5.96

Prosite motif: PS01076 ACETATE_KINASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYKILVINPGATSTKLSLFEDEKEVISESIEHSVSEIENCKTIMDQLPFRKKCVEDFLKR
CEEEEEECCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
YNISINELDAIAARGGILPPMKSGTYQVNEDMVNYLKTKTRVEHASNLAAVIAFELSKNS
HCCCHHHHHHHHHCCCCCCCCCCCCEEECHHHHHHHHHHHHHHHHHCEEEEEEEEECCCC
SKGLPVFITDPISVDEFIPEARISGIPQLERHSLFHALNMKIVARFAAKELNKEYEKCNF
CCCCCEEEECCCCHHHHCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHE
VIAHLGGGISVGAQRKGLMIDVNNATDEGPFSSQRTGELPMGDLAKWIFKNMHSYSKNDV
EEEECCCCEEECCCCCCEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCH
KSTFVGKGGLFAYLKTASLKDALKLAEKDEYAKLIVEAMAYQVSKEIGGMAAILGGDLDA
HHHHCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCE
IILTGGMAHSDYFVELIKRRIDKLGVVMVYPGAYEMEGLALGALRALEQLEDTKIWEGEN
EEEECCCCCHHHHHHHHHHHHHHCCEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCCC
IL
CC
>Mature Secondary Structure
MYKILVINPGATSTKLSLFEDEKEVISESIEHSVSEIENCKTIMDQLPFRKKCVEDFLKR
CEEEEEECCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
YNISINELDAIAARGGILPPMKSGTYQVNEDMVNYLKTKTRVEHASNLAAVIAFELSKNS
HCCCHHHHHHHHHCCCCCCCCCCCCEEECHHHHHHHHHHHHHHHHHCEEEEEEEEECCCC
SKGLPVFITDPISVDEFIPEARISGIPQLERHSLFHALNMKIVARFAAKELNKEYEKCNF
CCCCCEEEECCCCHHHHCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHE
VIAHLGGGISVGAQRKGLMIDVNNATDEGPFSSQRTGELPMGDLAKWIFKNMHSYSKNDV
EEEECCCCEEECCCCCCEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCH
KSTFVGKGGLFAYLKTASLKDALKLAEKDEYAKLIVEAMAYQVSKEIGGMAAILGGDLDA
HHHHCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCE
IILTGGMAHSDYFVELIKRRIDKLGVVMVYPGAYEMEGLALGALRALEQLEDTKIWEGEN
EEEECCCCCHHHHHHHHHHHHHHCCEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCCC
IL
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10360571 [H]