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The map label for this gene is ilvK [H]

Identifier: 160877249

GI number: 160877249

Start: 4913322

End: 4914413

Strand: Reverse

Name: ilvK [H]

Synonym: Sbal195_4146

Alternate gene names: 160877249

Gene position: 4914413-4913322 (Counterclockwise)

Preceding gene: 160877252

Following gene: 160877241

Centisome position: 91.9

GC content: 48.9

Gene sequence:

>1092_bases
ATGGAAATAACTTACAATTTAAAACCTGCGTCCGAGCGCCGTACTGAGCAATTCAAGCCCGAGGGGAATGTGGGCTTTGG
TAGCCTCAGAACCGATCATATGTTTTTGATGGATTATCATGACGGACAATGGTGCGATCCGCGCATCGTTCCTTATGGTC
CATTTGAAATGGCACCGGGTGCAATGGCGTTGCATTATGGCCAGTCGATCTTCGAAGGCGCGAAAGCCTTTATGCACGAA
GATGGCGAGATTTATACTTTCCGCATTAATAAGAATGCCGAGCGGATGAATCGCTCCGCCGATATCGTCTGTATTCCTAA
TATCGATGAACAAGTGCAGATGGATGCGATCAATGCGTTGATCGATGTCGATCGCCTGTGGTTCCCGATGCAAGAAGGTG
CGTGTCTGTATATTCGCCCCTTTATTTTTGCGACCGAAGACCGTCTCTCTGTTAGCCCAAGCACGCGTTATACCTTCTGT
GTGGTATTAAGCCCAAGTGGTGCTTATTACGCAGCGGGCGTGAACAAAGGCATACGCTTATTGATCAGCAAAACTTTCCA
TCGCGCGGTATCGGGTGGCACTGGTGCCTCAAAAGCGGCGGGTAACTATGCGGCGTCGTTGCGTGCGGGTAAAGCCGCGG
CAGCCTTTGGTGCATCGCAAGTGTTATATCTCGACTCGACCAATCAACAGATTGAAGAAGCGGGCGCGATGAACCATTTC
CACATATTAAAAGATGGCACTGTGATCATTCCGACCTTCACCGATACCATTCTTAAGTCGATCACCTCGCAATCCATCAT
GGAATTGGGTGAGATGCTTGGTTGTGAAGTGCGCCAAGAGACTGTCATGCTTGATCAATTTATCGCCGATATCGAATCTG
GTGAAATTATCGAAGCGGGTGGTTTCGGGACTGCGGCTGTGGTTTCAGCTGTGGGTTCCTACATTTTTGAAGATGGCCAC
ATAGTCACTGTCGGTAATGGTGAAGTGGGCGAGCATGTTCAGCGCATCTACAAACTGTATACCGAAATCCAAAAGGGCCA
CATTCAAGGTCCTGAAGGCTGGGTTAAGCGTGTTGAACGTTTAGCAAAGTAA

Upstream 100 bases:

>100_bases
CCTGCATCTTCAGATTGTTTGGGTATAGATATGCGCGCCCCTCTTGACTTTATTCCTGTCGCTCCCTAGTCTGCTTGTTC
AGTAATCAAGGTGGTATGCG

Downstream 100 bases:

>100_bases
GGATGTTGTGGTGATGATTAAAGGCTCCTAGGAGCCTTTTTTATTGGCTATTTTTGACGATGACATTTGTAGGCTTCGCC
TCAAATCAAACCAAATCAAA

Product: branched-chain amino acid aminotransferase

Products: NA

Alternate protein names: BCAT 2; Vegetative protein 85; VEG85 [H]

Number of amino acids: Translated: 363; Mature: 363

Protein sequence:

>363_residues
MEITYNLKPASERRTEQFKPEGNVGFGSLRTDHMFLMDYHDGQWCDPRIVPYGPFEMAPGAMALHYGQSIFEGAKAFMHE
DGEIYTFRINKNAERMNRSADIVCIPNIDEQVQMDAINALIDVDRLWFPMQEGACLYIRPFIFATEDRLSVSPSTRYTFC
VVLSPSGAYYAAGVNKGIRLLISKTFHRAVSGGTGASKAAGNYAASLRAGKAAAAFGASQVLYLDSTNQQIEEAGAMNHF
HILKDGTVIIPTFTDTILKSITSQSIMELGEMLGCEVRQETVMLDQFIADIESGEIIEAGGFGTAAVVSAVGSYIFEDGH
IVTVGNGEVGEHVQRIYKLYTEIQKGHIQGPEGWVKRVERLAK

Sequences:

>Translated_363_residues
MEITYNLKPASERRTEQFKPEGNVGFGSLRTDHMFLMDYHDGQWCDPRIVPYGPFEMAPGAMALHYGQSIFEGAKAFMHE
DGEIYTFRINKNAERMNRSADIVCIPNIDEQVQMDAINALIDVDRLWFPMQEGACLYIRPFIFATEDRLSVSPSTRYTFC
VVLSPSGAYYAAGVNKGIRLLISKTFHRAVSGGTGASKAAGNYAASLRAGKAAAAFGASQVLYLDSTNQQIEEAGAMNHF
HILKDGTVIIPTFTDTILKSITSQSIMELGEMLGCEVRQETVMLDQFIADIESGEIIEAGGFGTAAVVSAVGSYIFEDGH
IVTVGNGEVGEHVQRIYKLYTEIQKGHIQGPEGWVKRVERLAK
>Mature_363_residues
MEITYNLKPASERRTEQFKPEGNVGFGSLRTDHMFLMDYHDGQWCDPRIVPYGPFEMAPGAMALHYGQSIFEGAKAFMHE
DGEIYTFRINKNAERMNRSADIVCIPNIDEQVQMDAINALIDVDRLWFPMQEGACLYIRPFIFATEDRLSVSPSTRYTFC
VVLSPSGAYYAAGVNKGIRLLISKTFHRAVSGGTGASKAAGNYAASLRAGKAAAAFGASQVLYLDSTNQQIEEAGAMNHF
HILKDGTVIIPTFTDTILKSITSQSIMELGEMLGCEVRQETVMLDQFIADIESGEIIEAGGFGTAAVVSAVGSYIFEDGH
IVTVGNGEVGEHVQRIYKLYTEIQKGHIQGPEGWVKRVERLAK

Specific function: Transaminates branched-chain amino acids and ketoglutarate [H]

COG id: COG0115

COG function: function code EH; Branched-chain amino acid aminotransferase/4-amino-4-deoxychorismate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Homo sapiens, GI50658084, Length=328, Percent_Identity=35.3658536585366, Blast_Score=204, Evalue=9e-53,
Organism=Homo sapiens, GI296010904, Length=338, Percent_Identity=35.5029585798817, Blast_Score=189, Evalue=5e-48,
Organism=Homo sapiens, GI38176287, Length=338, Percent_Identity=35.5029585798817, Blast_Score=189, Evalue=5e-48,
Organism=Homo sapiens, GI296010906, Length=338, Percent_Identity=35.5029585798817, Blast_Score=188, Evalue=5e-48,
Organism=Homo sapiens, GI258614015, Length=285, Percent_Identity=35.0877192982456, Blast_Score=166, Evalue=3e-41,
Organism=Homo sapiens, GI296010902, Length=337, Percent_Identity=30.8605341246291, Blast_Score=145, Evalue=7e-35,
Organism=Homo sapiens, GI296010900, Length=338, Percent_Identity=30.7692307692308, Blast_Score=144, Evalue=9e-35,
Organism=Escherichia coli, GI48994963, Length=324, Percent_Identity=30.8641975308642, Blast_Score=118, Evalue=5e-28,
Organism=Caenorhabditis elegans, GI17568601, Length=372, Percent_Identity=31.7204301075269, Blast_Score=180, Evalue=1e-45,
Organism=Caenorhabditis elegans, GI17565728, Length=351, Percent_Identity=34.7578347578348, Blast_Score=168, Evalue=4e-42,
Organism=Saccharomyces cerevisiae, GI6322608, Length=315, Percent_Identity=33.015873015873, Blast_Score=169, Evalue=7e-43,
Organism=Saccharomyces cerevisiae, GI6322002, Length=373, Percent_Identity=30.0268096514745, Blast_Score=167, Evalue=2e-42,
Organism=Drosophila melanogaster, GI24641779, Length=349, Percent_Identity=33.5243553008596, Blast_Score=184, Evalue=8e-47,

Paralogues:

None

Copy number: 2342 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 11,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001544
- InterPro:   IPR018300
- InterPro:   IPR005786 [H]

Pfam domain/function: PF01063 Aminotran_4 [H]

EC number: =2.6.1.42 [H]

Molecular weight: Translated: 39991; Mature: 39991

Theoretical pI: Translated: 5.25; Mature: 5.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEITYNLKPASERRTEQFKPEGNVGFGSLRTDHMFLMDYHDGQWCDPRIVPYGPFEMAPG
CEEEEECCCCHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCCCCCCCEECCCCCHHCCCC
AMALHYGQSIFEGAKAFMHEDGEIYTFRINKNAERMNRSADIVCIPNIDEQVQMDAINAL
HHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCHHHCCCCCCEEEECCCCHHHHHHHHHHH
IDVDRLWFPMQEGACLYIRPFIFATEDRLSVSPSTRYTFCVVLSPSGAYYAAGVNKGIRL
HHHHHHCCCCCCCCEEEEEEEEEEECCCEECCCCCCEEEEEEECCCCCEEEECCCCCHHH
LISKTFHRAVSGGTGASKAAGNYAASLRAGKAAAAFGASQVLYLDSTNQQIEEAGAMNHF
HHHHHHHHHHCCCCCCCCCCCCHHHHHHCCCHHHHCCCCEEEEEECCCHHHHHHCCCCEE
HILKDGTVIIPTFTDTILKSITSQSIMELGEMLGCEVRQETVMLDQFIADIESGEIIEAG
EEEECCCEEEECHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCEEEEC
GFGTAAVVSAVGSYIFEDGHIVTVGNGEVGEHVQRIYKLYTEIQKGHIQGPEGWVKRVER
CCCHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
LAK
HCC
>Mature Secondary Structure
MEITYNLKPASERRTEQFKPEGNVGFGSLRTDHMFLMDYHDGQWCDPRIVPYGPFEMAPG
CEEEEECCCCHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCCCCCCCEECCCCCHHCCCC
AMALHYGQSIFEGAKAFMHEDGEIYTFRINKNAERMNRSADIVCIPNIDEQVQMDAINAL
HHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCHHHCCCCCCEEEECCCCHHHHHHHHHHH
IDVDRLWFPMQEGACLYIRPFIFATEDRLSVSPSTRYTFCVVLSPSGAYYAAGVNKGIRL
HHHHHHCCCCCCCCEEEEEEEEEEECCCEECCCCCCEEEEEEECCCCCEEEECCCCCHHH
LISKTFHRAVSGGTGASKAAGNYAASLRAGKAAAAFGASQVLYLDSTNQQIEEAGAMNHF
HHHHHHHHHHCCCCCCCCCCCCHHHHHHCCCHHHHCCCCEEEEEECCCHHHHHHCCCCEE
HILKDGTVIIPTFTDTILKSITSQSIMELGEMLGCEVRQETVMLDQFIADIESGEIIEAG
EEEECCCEEEECHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCEEEEC
GFGTAAVVSAVGSYIFEDGHIVTVGNGEVGEHVQRIYKLYTEIQKGHIQGPEGWVKRVER
CCCHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
LAK
HCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377; 7934828; 9298659 [H]