Click here to switch to the map view.

The map label for this gene is metK [H]

Identifier: 160876766

GI number: 160876766

Start: 4319473

End: 4320624

Strand: Direct

Name: metK [H]

Synonym: Sbal195_3660

Alternate gene names: 160876766

Gene position: 4319473-4320624 (Clockwise)

Preceding gene: 160876756

Following gene: 160876769

Centisome position: 80.78

GC content: 48.52

Gene sequence:

>1152_bases
ATGGCAAAGCACTTGTTTACTTCTGAGTCGGTCTCAGAAGGTCATCCCGATAAGATTGCAGATCAGATTTCTGATGCTGT
GCTAGATGCAATTCTGGCTCAAGATCCAAAAGCACGCGTGGCGTGTGAAACTTATGTCAAAACTGGCATGGTATTAGTCG
GTGGCGAAGTCACGACTTCTGCTTGGGTTGATATCGAAGAAATCACCCGTAAAACGGTGCGTGAAATTGGTTATACCCAC
TCAGATATGGGTTTTGATGCAGACTCGTGTGCGGTATTAAACGCCATTGGTAAGCAATCACCTGATATCAATCAAGGTGT
AGACCGTGCTGACCCTGCTGAGCAAGGTGCAGGCGACCAAGGGTTAATGTTTGGCTATGCCAACAACGAAACCGACGTCC
TAATGCCAGCGCCTATCACTTATGCCCACGCATTAGTGAAGCGTCAGTCTGAAGTGCGTAAAAACGGCACCCTGCCTTGG
TTACGCCCAGATGCGAAAAGCCAAGTCACGTTTGCCTATGATGATGGCAAAATTGTCGGTATCGATGCCGTCGTATTGTC
GACTCAGCACAGAGATGACGTTTCACAAGCCGACTTAATTGAAGGCGTGATGGAAACCATCATCAAACCAGTATTACCGG
CTCAATGGCTAAACAAAGACACTAAGTTCTTTATCAACCCAACTGGCCGTTTTGTTATCGGTGGTCCAGTAGGCGATTGC
GGCTTAACCGGTCGTAAGATCATCGTTGACACCTACGGCGGCATGGCCCGTCATGGTGGTGGCGCATTCTCTGGTAAAGA
TCCATCAAAAGTTGACCGTAGCGCGGCATACGCTGCCCGTTATGTGGCAAAAAACATAGTGGCAGCAGGTCTTGCTGATC
GCTGTGAAATCCAAGTGTCTTACGCTATTGGTGTAGCAGAGCCTACTTCTATCAGCATCGAGACCTTCGGTACGGGTAAA
GTGTCTGAAGATTTGCTGATCAAGTTAGTACGTCAACACTTCGAACTGCGTCCATACGGTTTAACGGCCATGCTAGATTT
AGCACGTCCGATTTATCAAGCGACTGCCGCTTATGGTCACTTTGGTCGTAACGAATTCCCTTGGGAAGCAACGGACAAAG
CTGAAGCACTGCGCGCTGACGCTGGTCTATAA

Upstream 100 bases:

>100_bases
TTCTCACTGGGGGTGATCGGCAATCAGATTTAAACTAAAATAGCCGTCTAGATGTAGATGCTTCTACACGTTTGAATGCT
AACGACGAGATTTTCCTACT

Downstream 100 bases:

>100_bases
TCGGTTTCGATTAAAACCCGCTAAGATTGCAAAATATAAAAACCGCTATCCAGCGGTTTTTTTATGCCTGAAATTTGGGT
TCCCCAAAGCAAGATAATTG

Product: S-adenosylmethionine synthetase

Products: NA

Alternate protein names: AdoMet synthase; MAT; Methionine adenosyltransferase [H]

Number of amino acids: Translated: 383; Mature: 382

Protein sequence:

>383_residues
MAKHLFTSESVSEGHPDKIADQISDAVLDAILAQDPKARVACETYVKTGMVLVGGEVTTSAWVDIEEITRKTVREIGYTH
SDMGFDADSCAVLNAIGKQSPDINQGVDRADPAEQGAGDQGLMFGYANNETDVLMPAPITYAHALVKRQSEVRKNGTLPW
LRPDAKSQVTFAYDDGKIVGIDAVVLSTQHRDDVSQADLIEGVMETIIKPVLPAQWLNKDTKFFINPTGRFVIGGPVGDC
GLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAAYAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSISIETFGTGK
VSEDLLIKLVRQHFELRPYGLTAMLDLARPIYQATAAYGHFGRNEFPWEATDKAEALRADAGL

Sequences:

>Translated_383_residues
MAKHLFTSESVSEGHPDKIADQISDAVLDAILAQDPKARVACETYVKTGMVLVGGEVTTSAWVDIEEITRKTVREIGYTH
SDMGFDADSCAVLNAIGKQSPDINQGVDRADPAEQGAGDQGLMFGYANNETDVLMPAPITYAHALVKRQSEVRKNGTLPW
LRPDAKSQVTFAYDDGKIVGIDAVVLSTQHRDDVSQADLIEGVMETIIKPVLPAQWLNKDTKFFINPTGRFVIGGPVGDC
GLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAAYAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSISIETFGTGK
VSEDLLIKLVRQHFELRPYGLTAMLDLARPIYQATAAYGHFGRNEFPWEATDKAEALRADAGL
>Mature_382_residues
AKHLFTSESVSEGHPDKIADQISDAVLDAILAQDPKARVACETYVKTGMVLVGGEVTTSAWVDIEEITRKTVREIGYTHS
DMGFDADSCAVLNAIGKQSPDINQGVDRADPAEQGAGDQGLMFGYANNETDVLMPAPITYAHALVKRQSEVRKNGTLPWL
RPDAKSQVTFAYDDGKIVGIDAVVLSTQHRDDVSQADLIEGVMETIIKPVLPAQWLNKDTKFFINPTGRFVIGGPVGDCG
LTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAAYAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSISIETFGTGKV
SEDLLIKLVRQHFELRPYGLTAMLDLARPIYQATAAYGHFGRNEFPWEATDKAEALRADAGL

Specific function: Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the e

COG id: COG0192

COG function: function code H; S-adenosylmethionine synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AdoMet synthase family [H]

Homologues:

Organism=Homo sapiens, GI5174529, Length=375, Percent_Identity=58.1333333333333, Blast_Score=417, Evalue=1e-117,
Organism=Homo sapiens, GI4557737, Length=375, Percent_Identity=58.1333333333333, Blast_Score=417, Evalue=1e-117,
Organism=Escherichia coli, GI1789311, Length=383, Percent_Identity=86.1618798955614, Blast_Score=687, Evalue=0.0,
Organism=Caenorhabditis elegans, GI25145633, Length=386, Percent_Identity=55.1813471502591, Blast_Score=407, Evalue=1e-114,
Organism=Caenorhabditis elegans, GI17538498, Length=372, Percent_Identity=56.1827956989247, Blast_Score=405, Evalue=1e-113,
Organism=Caenorhabditis elegans, GI17538494, Length=376, Percent_Identity=55.5851063829787, Blast_Score=404, Evalue=1e-113,
Organism=Caenorhabditis elegans, GI17551082, Length=387, Percent_Identity=53.4883720930233, Blast_Score=399, Evalue=1e-111,
Organism=Caenorhabditis elegans, GI32565905, Length=376, Percent_Identity=50.531914893617, Blast_Score=344, Evalue=4e-95,
Organism=Caenorhabditis elegans, GI32565911, Length=325, Percent_Identity=52.3076923076923, Blast_Score=329, Evalue=2e-90,
Organism=Caenorhabditis elegans, GI25145635, Length=339, Percent_Identity=51.3274336283186, Blast_Score=329, Evalue=2e-90,
Organism=Caenorhabditis elegans, GI32565903, Length=298, Percent_Identity=52.6845637583893, Blast_Score=298, Evalue=4e-81,
Organism=Caenorhabditis elegans, GI32565909, Length=87, Percent_Identity=60.9195402298851, Blast_Score=110, Evalue=8e-25,
Organism=Caenorhabditis elegans, GI32565907, Length=46, Percent_Identity=76.0869565217391, Blast_Score=75, Evalue=8e-14,
Organism=Saccharomyces cerevisiae, GI6320710, Length=376, Percent_Identity=56.9148936170213, Blast_Score=414, Evalue=1e-117,
Organism=Saccharomyces cerevisiae, GI6323209, Length=370, Percent_Identity=57.027027027027, Blast_Score=407, Evalue=1e-114,
Organism=Drosophila melanogaster, GI45552159, Length=380, Percent_Identity=53.9473684210526, Blast_Score=377, Evalue=1e-105,
Organism=Drosophila melanogaster, GI24580529, Length=380, Percent_Identity=53.9473684210526, Blast_Score=377, Evalue=1e-105,
Organism=Drosophila melanogaster, GI24580537, Length=380, Percent_Identity=53.9473684210526, Blast_Score=377, Evalue=1e-105,
Organism=Drosophila melanogaster, GI24580533, Length=380, Percent_Identity=53.9473684210526, Blast_Score=377, Evalue=1e-105,
Organism=Drosophila melanogaster, GI24580535, Length=380, Percent_Identity=53.9473684210526, Blast_Score=377, Evalue=1e-105,
Organism=Drosophila melanogaster, GI24580531, Length=380, Percent_Identity=53.9473684210526, Blast_Score=377, Evalue=1e-105,
Organism=Drosophila melanogaster, GI24580527, Length=379, Percent_Identity=53.0343007915567, Blast_Score=371, Evalue=1e-103,
Organism=Drosophila melanogaster, GI24580525, Length=379, Percent_Identity=53.0343007915567, Blast_Score=371, Evalue=1e-103,
Organism=Drosophila melanogaster, GI24580541, Length=378, Percent_Identity=50.7936507936508, Blast_Score=348, Evalue=3e-96,
Organism=Drosophila melanogaster, GI24580539, Length=120, Percent_Identity=56.6666666666667, Blast_Score=131, Evalue=7e-31,

Paralogues:

None

Copy number: 7,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022631
- InterPro:   IPR022630
- InterPro:   IPR022629
- InterPro:   IPR022628
- InterPro:   IPR002133
- InterPro:   IPR022636 [H]

Pfam domain/function: PF02773 S-AdoMet_synt_C; PF02772 S-AdoMet_synt_M; PF00438 S-AdoMet_synt_N [H]

EC number: =2.5.1.6 [H]

Molecular weight: Translated: 41264; Mature: 41133

Theoretical pI: Translated: 5.03; Mature: 5.03

Prosite motif: PS00376 ADOMET_SYNTHETASE_1 ; PS00377 ADOMET_SYNTHETASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKHLFTSESVSEGHPDKIADQISDAVLDAILAQDPKARVACETYVKTGMVLVGGEVTTS
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCEEEECCCCCCH
AWVDIEEITRKTVREIGYTHSDMGFDADSCAVLNAIGKQSPDINQGVDRADPAEQGAGDQ
HCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCCCHHCCCCCCCCHHHCCCCC
GLMFGYANNETDVLMPAPITYAHALVKRQSEVRKNGTLPWLRPDAKSQVTFAYDDGKIVG
CEEEEECCCCCCEEECCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCEEEE
IDAVVLSTQHRDDVSQADLIEGVMETIIKPVLPAQWLNKDTKFFINPTGRFVIGGPVGDC
EEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCHHHCCCCCEEEECCCCCEEECCCCCCC
GLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAAYAARYVAKNIVAAGLADRCEIQVS
CCCCCEEEEECCCCCHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEE
YAIGVAEPTSISIETFGTGKVSEDLLIKLVRQHFELRPYGLTAMLDLARPIYQATAAYGH
EEEECCCCCEEEEEECCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHC
FGRNEFPWEATDKAEALRADAGL
CCCCCCCCCCCCHHHHHHHCCCC
>Mature Secondary Structure 
AKHLFTSESVSEGHPDKIADQISDAVLDAILAQDPKARVACETYVKTGMVLVGGEVTTS
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCEEEECCCCCCH
AWVDIEEITRKTVREIGYTHSDMGFDADSCAVLNAIGKQSPDINQGVDRADPAEQGAGDQ
HCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCCCHHCCCCCCCCHHHCCCCC
GLMFGYANNETDVLMPAPITYAHALVKRQSEVRKNGTLPWLRPDAKSQVTFAYDDGKIVG
CEEEEECCCCCCEEECCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCEEEE
IDAVVLSTQHRDDVSQADLIEGVMETIIKPVLPAQWLNKDTKFFINPTGRFVIGGPVGDC
EEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCHHHCCCCCEEEECCCCCEEECCCCCCC
GLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAAYAARYVAKNIVAAGLADRCEIQVS
CCCCCEEEEECCCCCHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEE
YAIGVAEPTSISIETFGTGKVSEDLLIKLVRQHFELRPYGLTAMLDLARPIYQATAAYGH
EEEECCCCCEEEEEECCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHC
FGRNEFPWEATDKAEALRADAGL
CCCCCCCCCCCCHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA