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The map label for this gene is prfB [H]

Identifier: 160876721

GI number: 160876721

Start: 4258579

End: 4259637

Strand: Reverse

Name: prfB [H]

Synonym: Sbal195_3615

Alternate gene names: 160876721

Gene position: 4259637-4258579 (Counterclockwise)

Preceding gene: 160876723

Following gene: 160876720

Centisome position: 79.66

GC content: 50.42

Gene sequence:

>1059_bases
TTGCCGAGCGCACGCAGCTTCTTAGGGGGTATCTTTGACTACGATGCCAAAAATGAGCGTCTAGAAGAAGTTACCCGTGA
GCTTGAAAGTTCAGAAGTGTGGAACGACCCAGAGCGCGCCCAAGCCTTAGGCAAAGAGCGCGCCAGCCTAGAAGCTGTGG
TCAAAACCATAGACGATATGGACTCAGGTCTTGAAGATGTTGAAGGCTTACTCGAGCTGGCTATTGAAGAGGAAGACGAA
GACACTTTCAACGAAACCAGTAAAGAGCTGGATTATCTCGAAAGCCGCCTTGCCGATCTCGAATTCCGCCGTATGTTCTC
CGGCCCGCAAGATGCGTCAAACTGTTATTTAGACATTCAGTCTGGCAGTGGTGGTACCGAAGCCCAAGATTGGGCCAACA
TGGTGCTGCGTATGTACCTGCGTTGGGGCGAAGCCCATGGTTTTAGCCCAGAGTTGATGGAAGTGACCGATGGTGACGTG
GCCGGTATTAAAGGCGCGACCATCAAGTTCACTGGCGAATATGCGTTCGGTTGGTTACGTACCGAAACGGGCGTGCACCG
CTTAGTGCGTAAGTCGCCATTCGACTCATCGGGTCGTCGTCATACGTCATTCTGCTCTGTGTTCGTTTATCCAGAAATCG
ACGACGATATCACTATCGATATCAACCCATCGGATCTGCGTATCGACACTTACCGCGCATCGGGCGCGGGTGGTCAGCAC
GTTAACAAAACAGAATCGGCGATTCGTATTACCCACTTACCGACTAACACAGTCGTGCAGTGTCAGAACGACCGTTCACA
GCACAAGAACAAAGATGCTGCGATGAAGCAGCTGAAAGCAAAACTGTTTGAGTTAGAAATGCTCAAGCAAAATGCCGACA
AACAGGCCGCAGAAGACGCTAAGTCTGATATCGGCTGGGGCAGTCAAATTCGCTCTTATGTATTAGACGATGCCCGCATT
AAAGACTTACGCACAGGCGTTGAAACCCGCAATACTCAAGCGGTACTCGATGGCGATCTGGATAAGTTTATCGAAGCCAG
TTTGAAATCTGGGCTGTAA

Upstream 100 bases:

>100_bases
TCATCGCCTCTTTTAGTGGAAGATCAATTAAAGCGGCCAAATAAAGAAAGTTGAAAATTCATGTTTGAAGTTAATCCAGT
AAAATTCAAAATTAAGGAGC

Downstream 100 bases:

>100_bases
TTAATTCAAGGTATAGACGAGAAATAAGATGACTGAACAAGTAATTGATGAGAACAAACTGATCGCCGAGCGCCGTGCCA
AGCTAGAAAGCATCCGCCCA

Product: peptide chain release factor 2

Products: NA

Alternate protein names: RF-2 [H]

Number of amino acids: Translated: 352; Mature: 351

Protein sequence:

>352_residues
MPSARSFLGGIFDYDAKNERLEEVTRELESSEVWNDPERAQALGKERASLEAVVKTIDDMDSGLEDVEGLLELAIEEEDE
DTFNETSKELDYLESRLADLEFRRMFSGPQDASNCYLDIQSGSGGTEAQDWANMVLRMYLRWGEAHGFSPELMEVTDGDV
AGIKGATIKFTGEYAFGWLRTETGVHRLVRKSPFDSSGRRHTSFCSVFVYPEIDDDITIDINPSDLRIDTYRASGAGGQH
VNKTESAIRITHLPTNTVVQCQNDRSQHKNKDAAMKQLKAKLFELEMLKQNADKQAAEDAKSDIGWGSQIRSYVLDDARI
KDLRTGVETRNTQAVLDGDLDKFIEASLKSGL

Sequences:

>Translated_352_residues
MPSARSFLGGIFDYDAKNERLEEVTRELESSEVWNDPERAQALGKERASLEAVVKTIDDMDSGLEDVEGLLELAIEEEDE
DTFNETSKELDYLESRLADLEFRRMFSGPQDASNCYLDIQSGSGGTEAQDWANMVLRMYLRWGEAHGFSPELMEVTDGDV
AGIKGATIKFTGEYAFGWLRTETGVHRLVRKSPFDSSGRRHTSFCSVFVYPEIDDDITIDINPSDLRIDTYRASGAGGQH
VNKTESAIRITHLPTNTVVQCQNDRSQHKNKDAAMKQLKAKLFELEMLKQNADKQAAEDAKSDIGWGSQIRSYVLDDARI
KDLRTGVETRNTQAVLDGDLDKFIEASLKSGL
>Mature_351_residues
PSARSFLGGIFDYDAKNERLEEVTRELESSEVWNDPERAQALGKERASLEAVVKTIDDMDSGLEDVEGLLELAIEEEDED
TFNETSKELDYLESRLADLEFRRMFSGPQDASNCYLDIQSGSGGTEAQDWANMVLRMYLRWGEAHGFSPELMEVTDGDVA
GIKGATIKFTGEYAFGWLRTETGVHRLVRKSPFDSSGRRHTSFCSVFVYPEIDDDITIDINPSDLRIDTYRASGAGGQHV
NKTESAIRITHLPTNTVVQCQNDRSQHKNKDAAMKQLKAKLFELEMLKQNADKQAAEDAKSDIGWGSQIRSYVLDDARIK
DLRTGVETRNTQAVLDGDLDKFIEASLKSGL

Specific function: Peptide chain release factor 2 directs the termination of translation in response to the peptide chain termination codons UGA and UAA [H]

COG id: COG1186

COG function: function code J; Protein chain release factor B

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family [H]

Homologues:

Organism=Homo sapiens, GI166795303, Length=311, Percent_Identity=32.7974276527331, Blast_Score=170, Evalue=2e-42,
Organism=Homo sapiens, GI34577120, Length=364, Percent_Identity=29.9450549450549, Blast_Score=164, Evalue=2e-40,
Organism=Homo sapiens, GI166795305, Length=218, Percent_Identity=33.9449541284404, Blast_Score=123, Evalue=3e-28,
Organism=Escherichia coli, GI2367172, Length=345, Percent_Identity=76.5217391304348, Blast_Score=561, Evalue=1e-161,
Organism=Escherichia coli, GI1787462, Length=346, Percent_Identity=38.150289017341, Blast_Score=209, Evalue=3e-55,
Organism=Caenorhabditis elegans, GI17542784, Length=278, Percent_Identity=29.4964028776978, Blast_Score=114, Evalue=9e-26,
Organism=Saccharomyces cerevisiae, GI6321295, Length=317, Percent_Identity=31.8611987381703, Blast_Score=141, Evalue=2e-34,
Organism=Drosophila melanogaster, GI19921226, Length=296, Percent_Identity=36.1486486486487, Blast_Score=159, Evalue=4e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR020853
- InterPro:   IPR004374 [H]

Pfam domain/function: PF03462 PCRF; PF00472 RF-1 [H]

EC number: NA

Molecular weight: Translated: 39426; Mature: 39295

Theoretical pI: Translated: 4.41; Mature: 4.41

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPSARSFLGGIFDYDAKNERLEEVTRELESSEVWNDPERAQALGKERASLEAVVKTIDDM
CCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
DSGLEDVEGLLELAIEEEDEDTFNETSKELDYLESRLADLEFRRMFSGPQDASNCYLDIQ
HCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEE
SGSGGTEAQDWANMVLRMYLRWGEAHGFSPELMEVTDGDVAGIKGATIKFTGEYAFGWLR
CCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCCCCCCCCCCEEEEECCEEEEEEE
TETGVHRLVRKSPFDSSGRRHTSFCSVFVYPEIDDDITIDINPSDLRIDTYRASGAGGQH
HHHHHHHHHHCCCCCCCCCCCCCEEEEEEECCCCCCEEEEECCCCEEEEEEECCCCCCCC
VNKTESAIRITHLPTNTVVQCQNDRSQHKNKDAAMKQLKAKLFELEMLKQNADKQAAEDA
CCCCCCEEEEEECCCCCEEEECCCHHHHCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
KSDIGWGSQIRSYVLDDARIKDLRTGVETRNTQAVLDGDLDKFIEASLKSGL
HHCCCHHHHHHHHHHCHHHHHHHHHCCCCCCCCEEECCCHHHHHHHHHHCCC
>Mature Secondary Structure 
PSARSFLGGIFDYDAKNERLEEVTRELESSEVWNDPERAQALGKERASLEAVVKTIDDM
CCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
DSGLEDVEGLLELAIEEEDEDTFNETSKELDYLESRLADLEFRRMFSGPQDASNCYLDIQ
HCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEE
SGSGGTEAQDWANMVLRMYLRWGEAHGFSPELMEVTDGDVAGIKGATIKFTGEYAFGWLR
CCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCCCCCCCCCCEEEEECCEEEEEEE
TETGVHRLVRKSPFDSSGRRHTSFCSVFVYPEIDDDITIDINPSDLRIDTYRASGAGGQH
HHHHHHHHHHCCCCCCCCCCCCCEEEEEEECCCCCCEEEEECCCCEEEEEEECCCCCCCC
VNKTESAIRITHLPTNTVVQCQNDRSQHKNKDAAMKQLKAKLFELEMLKQNADKQAAEDA
CCCCCCEEEEEECCCCCEEEECCCHHHHCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
KSDIGWGSQIRSYVLDDARIKDLRTGVETRNTQAVLDGDLDKFIEASLKSGL
HHCCCHHHHHHHHHHCHHHHHHHHHCCCCCCCCEEECCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA