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The map label for this gene is rpoS [H]

Identifier: 160876377

GI number: 160876377

Start: 3852945

End: 3853925

Strand: Reverse

Name: rpoS [H]

Synonym: Sbal195_3271

Alternate gene names: 160876377

Gene position: 3853925-3852945 (Counterclockwise)

Preceding gene: 160876378

Following gene: 160876375

Centisome position: 72.07

GC content: 42.1

Gene sequence:

>981_bases
ATGAGCCGCATAAAAAGCACTGCCGCAGAAGCACTAGTAGATTTATCCGTAGACACTGTTGACTTCGATCTTGAAAAAGA
AGAAGTTGCATCCGATTTAGTTCAAGAGCTAGGAATAGAGCAACAGGTTCAAGATGACCTGCAAAAAAATCTTGATGCCA
CTCAACTTTACCTAGGTGAGATTGGTTTTTCCCCACTGCTTAGCGCAGAAGAAGAAGTTTACTTTTCCCGTAAAGCCTTA
AAAGGCTGCGCAAAATCCCGTAATCGCATGATTGAAAGTAATTTACGTCTGGTCGTAAAGATTGCCCGTCGTTACAACAA
TCGTGGTTTAGCACTGCTTGATCTTATCGAAGAAGGCAACTTAGGTTTAATTCGTGCCGTTGAAAAATTTGACCCTGAAA
GAGGTTTCCGTTTTTCAACCTATGCGACTTGGTGGATTAGACAAACCATTGAGCGCGCTATTATGAATCAGACTCGTACG
ATTCGTTTGCCTATTCATGTTGTTAAAGAACTTAACGTTTACTTGCGTACCGCTCGCGAGTTAGCACAAAAACTCGATCA
CGAACCAACGGCCGAAGAAATTGCCGAAAAACTACAAGTGTCGAGTGCAGATGTCAGCCGTATGCTGAAGTTGAATGAGA
AAATCACCTCTGTCGATACGCCTTTGGGGGGCGATAATGACAAGGCGCTGCTGGATATTTTAGCCGATGATGATAACGTT
GGCCCTGACTATAAAGTGCAAGATGAAGATATTTCTAATTCAGTGGTTAAATGGCTCAATGAGTTAAACACTAAACAAAG
AGAAGTGTTAGCCCGCCGTTTTGGTCTGTTAGGTTATGAACCTTCTACACTCGAAGATGTGGGTGCTGAGATTGGTTTGA
CTCGTGAACGTGTACGTCAAATCCAAGTAGAAGCATTGAAACGTTTACGCGACTTATTAGGATCTCAAGGTTTATCTGTT
GAAGCATTGTTTAGAAACTAA

Upstream 100 bases:

>100_bases
CTTATTTACCCAAACAATGATTGCTTGGGGCCTTGTTGGCAAATGGAGGATAGGAAATTGCACTAATTCAGCAGTTTAAT
CAGGTTTGGGAGATTTAATC

Downstream 100 bases:

>100_bases
TTATTTCGAAACTAATAAATGAGAAACTAAGATCAGTTGTTCGTTGATCTCTATGCCTAGCTTCTAGCTAAGGTCTAGTG
ATTTAGTCTAGTGTTTAGGT

Product: RpoD family RNA polymerase sigma factor

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 326; Mature: 325

Protein sequence:

>326_residues
MSRIKSTAAEALVDLSVDTVDFDLEKEEVASDLVQELGIEQQVQDDLQKNLDATQLYLGEIGFSPLLSAEEEVYFSRKAL
KGCAKSRNRMIESNLRLVVKIARRYNNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMNQTRT
IRLPIHVVKELNVYLRTARELAQKLDHEPTAEEIAEKLQVSSADVSRMLKLNEKITSVDTPLGGDNDKALLDILADDDNV
GPDYKVQDEDISNSVVKWLNELNTKQREVLARRFGLLGYEPSTLEDVGAEIGLTRERVRQIQVEALKRLRDLLGSQGLSV
EALFRN

Sequences:

>Translated_326_residues
MSRIKSTAAEALVDLSVDTVDFDLEKEEVASDLVQELGIEQQVQDDLQKNLDATQLYLGEIGFSPLLSAEEEVYFSRKAL
KGCAKSRNRMIESNLRLVVKIARRYNNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMNQTRT
IRLPIHVVKELNVYLRTARELAQKLDHEPTAEEIAEKLQVSSADVSRMLKLNEKITSVDTPLGGDNDKALLDILADDDNV
GPDYKVQDEDISNSVVKWLNELNTKQREVLARRFGLLGYEPSTLEDVGAEIGLTRERVRQIQVEALKRLRDLLGSQGLSV
EALFRN
>Mature_325_residues
SRIKSTAAEALVDLSVDTVDFDLEKEEVASDLVQELGIEQQVQDDLQKNLDATQLYLGEIGFSPLLSAEEEVYFSRKALK
GCAKSRNRMIESNLRLVVKIARRYNNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMNQTRTI
RLPIHVVKELNVYLRTARELAQKLDHEPTAEEIAEKLQVSSADVSRMLKLNEKITSVDTPLGGDNDKALLDILADDDNVG
PDYKVQDEDISNSVVKWLNELNTKQREVLARRFGLLGYEPSTLEDVGAEIGLTRERVRQIQVEALKRLRDLLGSQGLSVE
ALFRN

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is required for the normal expression of the enterotoxin, yst, in the stationary phase [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789098, Length=302, Percent_Identity=77.1523178807947, Blast_Score=465, Evalue=1e-132,
Organism=Escherichia coli, GI1789448, Length=271, Percent_Identity=40.2214022140221, Blast_Score=205, Evalue=4e-54,
Organism=Escherichia coli, GI1789871, Length=279, Percent_Identity=29.7491039426523, Blast_Score=99, Evalue=5e-22,
Organism=Escherichia coli, GI1788231, Length=211, Percent_Identity=29.3838862559242, Blast_Score=65, Evalue=4e-12,

Paralogues:

None

Copy number: <10 (log phase) 250 (stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR012761
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 36985; Mature: 36853

Theoretical pI: Translated: 4.74; Mature: 4.74

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRIKSTAAEALVDLSVDTVDFDLEKEEVASDLVQELGIEQQVQDDLQKNLDATQLYLGE
CCHHHHHHHHHHHHCCCCCEECCCCHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHH
IGFSPLLSAEEEVYFSRKALKGCAKSRNRMIESNLRLVVKIARRYNNRGLALLDLIEEGN
CCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEHHHCCC
LGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMNQTRTIRLPIHVVKELNVYLRTARE
CHHHHHHHHCCCCCCCEEHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHH
LAQKLDHEPTAEEIAEKLQVSSADVSRMLKLNEKITSVDTPLGGDNDKALLDILADDDNV
HHHHCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHEEEEECCCCC
GPDYKVQDEDISNSVVKWLNELNTKQREVLARRFGLLGYEPSTLEDVGAEIGLTRERVRQ
CCCCEECCCHHHHHHHHHHHHHCHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCHHHHHH
IQVEALKRLRDLLGSQGLSVEALFRN
HHHHHHHHHHHHHCCCCCEEEEECCC
>Mature Secondary Structure 
SRIKSTAAEALVDLSVDTVDFDLEKEEVASDLVQELGIEQQVQDDLQKNLDATQLYLGE
CHHHHHHHHHHHHCCCCCEECCCCHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHH
IGFSPLLSAEEEVYFSRKALKGCAKSRNRMIESNLRLVVKIARRYNNRGLALLDLIEEGN
CCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEHHHCCC
LGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMNQTRTIRLPIHVVKELNVYLRTARE
CHHHHHHHHCCCCCCCEEHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHH
LAQKLDHEPTAEEIAEKLQVSSADVSRMLKLNEKITSVDTPLGGDNDKALLDILADDDNV
HHHHCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHEEEEECCCCC
GPDYKVQDEDISNSVVKWLNELNTKQREVLARRFGLLGYEPSTLEDVGAEIGLTRERVRQ
CCCCEECCCHHHHHHHHHHHHHCHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCHHHHHH
IQVEALKRLRDLLGSQGLSVEALFRN
HHHHHHHHHHHHHCCCCCEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7729893 [H]