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The map label for this gene is mscS [H]

Identifier: 160876277

GI number: 160876277

Start: 3745801

End: 3746628

Strand: Direct

Name: mscS [H]

Synonym: Sbal195_3171

Alternate gene names: 160876277

Gene position: 3745801-3746628 (Clockwise)

Preceding gene: 160876276

Following gene: 160876278

Centisome position: 70.05

GC content: 42.03

Gene sequence:

>828_bases
ATGGAAAACCTTGAAGGTTTATTAAAGCAAGCACCGGATCTGATTGTGACTTATGGCTTAAAAGTTTTATTTGCCATTAT
CATTTTCTTTGTTGGTAAATACTTTGCCGGCGTAGCGCAAAAGCTGGTTCGTAAATTACTGAACAGCCGTAAAGTTGATC
CGACCGTTGTTTCATTTATCGCAAACCTCGCTTGGGCAATTGTGTTTGTGTTCGCAGTAATTGCCACCTTAGGCCAAATT
GGCGTACAAACCGCATCATTGGTAGCCGTTATCGGTGCAGCTGGTTTAGCTGTGGGTTTAGCGCTACAGGGTTCTCTGTC
TAACTTCGCATCGGGCGTGTTAATGGTGTTATTCCGCCCTTGTCGTGTTGGTGACTATATTGAAGCCGCTGGCATTGCCG
GTACTGTTGATGAAATCACTATATTCTCAACCAAACTTCGCACACCAGATAACAAGGTTATTGTGGCGCCTAATTCATCC
ATCATGAATGGCACTATCACTAACTATTCAGCAATGGATACTCGCCGTATCGATTTAGTGATTGGCGTATCTTATTCTGC
CGATATCGCACTCACGAAGAAAATCTTAACTGAGATTTTAGACAACAACCAATATGTGTTAAAAGATCCATCATATACGG
TTGGACTGGCTGAATTAGCGAACTCTTCAATTAATTTCGTGGTTCGTCCTTGGGTAAAAACGGCTGATTATTGGACGGCT
CGTTTCCAACTGCTTGAGCAAATCAAAAATGCATTAGATGCAGCAGATATCGGTATTCCATTCCCACAAATGGATATTCA
TGTCAAAGAATTGCCACTCAGCAAATAA

Upstream 100 bases:

>100_bases
CGGACAACCGTTCTAGCGAAGGGCGCAGTTTAAATCGACGAGTACAATTTATCGTCAATAATAAATAGTTTTAGTTAGCG
TATATTTAGGGAGTTATTCA

Downstream 100 bases:

>100_bases
CCCTAAATGAATAACAAAGGTCGGCAATTGCCGGCCTTTTTTTATGGTTCTGCATAGCATTTTAGCGTACCTTAAAGGAC
AGATATTAGCGAAGGCACAG

Product: mechanosensitive ion channel protein MscS

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 275; Mature: 275

Protein sequence:

>275_residues
MENLEGLLKQAPDLIVTYGLKVLFAIIIFFVGKYFAGVAQKLVRKLLNSRKVDPTVVSFIANLAWAIVFVFAVIATLGQI
GVQTASLVAVIGAAGLAVGLALQGSLSNFASGVLMVLFRPCRVGDYIEAAGIAGTVDEITIFSTKLRTPDNKVIVAPNSS
IMNGTITNYSAMDTRRIDLVIGVSYSADIALTKKILTEILDNNQYVLKDPSYTVGLAELANSSINFVVRPWVKTADYWTA
RFQLLEQIKNALDAADIGIPFPQMDIHVKELPLSK

Sequences:

>Translated_275_residues
MENLEGLLKQAPDLIVTYGLKVLFAIIIFFVGKYFAGVAQKLVRKLLNSRKVDPTVVSFIANLAWAIVFVFAVIATLGQI
GVQTASLVAVIGAAGLAVGLALQGSLSNFASGVLMVLFRPCRVGDYIEAAGIAGTVDEITIFSTKLRTPDNKVIVAPNSS
IMNGTITNYSAMDTRRIDLVIGVSYSADIALTKKILTEILDNNQYVLKDPSYTVGLAELANSSINFVVRPWVKTADYWTA
RFQLLEQIKNALDAADIGIPFPQMDIHVKELPLSK
>Mature_275_residues
MENLEGLLKQAPDLIVTYGLKVLFAIIIFFVGKYFAGVAQKLVRKLLNSRKVDPTVVSFIANLAWAIVFVFAVIATLGQI
GVQTASLVAVIGAAGLAVGLALQGSLSNFASGVLMVLFRPCRVGDYIEAAGIAGTVDEITIFSTKLRTPDNKVIVAPNSS
IMNGTITNYSAMDTRRIDLVIGVSYSADIALTKKILTEILDNNQYVLKDPSYTVGLAELANSSINFVVRPWVKTADYWTA
RFQLLEQIKNALDAADIGIPFPQMDIHVKELPLSK

Specific function: Mechanosensitive channel that participates in the regulation of osmotic pressure changes within the cell. Forms an ion channel of 1.0 nanosiemens conductance with a slight preference for anions. The channel is sensitive to voltage [H]

COG id: COG0668

COG function: function code M; Small-conductance mechanosensitive channel

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mscS (TC 1.A.23) family [H]

Homologues:

Organism=Escherichia coli, GI1789291, Length=262, Percent_Identity=48.0916030534351, Blast_Score=247, Evalue=7e-67,
Organism=Escherichia coli, GI1786670, Length=263, Percent_Identity=26.615969581749, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI1787591, Length=266, Percent_Identity=27.4436090225564, Blast_Score=87, Evalue=8e-19,
Organism=Escherichia coli, GI2367355, Length=260, Percent_Identity=23.4615384615385, Blast_Score=75, Evalue=4e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010920
- InterPro:   IPR011066
- InterPro:   IPR006685
- InterPro:   IPR006686
- InterPro:   IPR011014
- InterPro:   IPR008910 [H]

Pfam domain/function: PF00924 MS_channel; PF05552 TM_helix [H]

EC number: NA

Molecular weight: Translated: 29767; Mature: 29767

Theoretical pI: Translated: 8.88; Mature: 8.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MENLEGLLKQAPDLIVTYGLKVLFAIIIFFVGKYFAGVAQKLVRKLLNSRKVDPTVVSFI
CCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
ANLAWAIVFVFAVIATLGQIGVQTASLVAVIGAAGLAVGLALQGSLSNFASGVLMVLFRP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
CRVGDYIEAAGIAGTVDEITIFSTKLRTPDNKVIVAPNSSIMNGTITNYSAMDTRRIDLV
CCCCCHHHHCCCCCCHHHEEEEEEEECCCCCEEEECCCCCCCCCCCCCCCCCCCEEEEEE
IGVSYSADIALTKKILTEILDNNQYVLKDPSYTVGLAELANSSINFVVRPWVKTADYWTA
EECCCCCCHHHHHHHHHHHHCCCCEEEECCCCEEEHHHHCCCCCCEEEECCHHHHHHHHH
RFQLLEQIKNALDAADIGIPFPQMDIHVKELPLSK
HHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCC
>Mature Secondary Structure
MENLEGLLKQAPDLIVTYGLKVLFAIIIFFVGKYFAGVAQKLVRKLLNSRKVDPTVVSFI
CCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
ANLAWAIVFVFAVIATLGQIGVQTASLVAVIGAAGLAVGLALQGSLSNFASGVLMVLFRP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
CRVGDYIEAAGIAGTVDEITIFSTKLRTPDNKVIVAPNSSIMNGTITNYSAMDTRRIDLV
CCCCCHHHHCCCCCCHHHEEEEEEEECCCCCEEEECCCCCCCCCCCCCCCCCCCEEEEEE
IGVSYSADIALTKKILTEILDNNQYVLKDPSYTVGLAELANSSINFVVRPWVKTADYWTA
EECCCCCCHHHHHHHHHHHHCCCCEEEECCCCEEEHHHHCCCCCCEEEECCHHHHHHHHH
RFQLLEQIKNALDAADIGIPFPQMDIHVKELPLSK
HHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 12542086 [H]