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The map label for this gene is wcaJ [H]

Identifier: 160876141

GI number: 160876141

Start: 3600630

End: 3601670

Strand: Reverse

Name: wcaJ [H]

Synonym: Sbal195_3032

Alternate gene names: 160876141

Gene position: 3601670-3600630 (Counterclockwise)

Preceding gene: 160876142

Following gene: 160876140

Centisome position: 67.36

GC content: 37.46

Gene sequence:

>1041_bases
ATGGCTTTAAGCCTACTAGTACCAGAACCTATTAACAATTTGGTTTATTGTATGAATATACGTGTAAATGAAACGGTAGA
TACTGAAGAGTTTCAACCAATTAAAACAGTAATTAAACGTTTTAAGTCAAAACGACAGGTACTCACTCAGGAAGTTATTT
ATGTCTATTGCCCTAAAGATAGGGAATTAGAAATCGGTGAAATTATTGATGACTATTCATTTGATATGGTCGTTAATGAC
TACAGCATTACTGATCTAAGTTGTAAAGTAATTTGCCACTTTGAAAGTGCGCGTTTAACTAAAGAACAACGACAGTTTTT
GGTTAAGGCTACTGAGCTTGGCGCTTGGGTTGAACCTCTGGTCAGTTATTTAGATGAAAGACTAGGCTACACCGAAGTGC
GTTTACTCCATAGTGGTTATTTTTTACATCAAAAAGCATTTTCTATTCTATCGACAAAGCGTACGCAGCTCGCTAAGAGA
GTTATTGATCTTTTTGCCGCTCTTTTACTCTGTACTATTGCAATACCGTTAGGATTATTAACTGCACTGGCAATAAAACT
TGAGAGTCCAGGTCCGGTTTTTTATAAGCAGCGCCGTACTGGTCAATATAATGATGAATTTGAAGTGATAAAATTTCGCT
CTATGCGTAACGATGCAGAAAAGTCGGGGGCGCAATGGGCAAGTCAGAATGATGCGCGTGTAACAAAAGTGGGTAACTTT
ATACGTAAAACCCGTATCGATGAATTACCGCAAATTATTAACATTCTAAAAGGCGAAATGTCGATTGTCGGTCCAAGACC
CGAGCGTGAAGTCTTTATCGCTGAGTTAGAAAAAGAAATTCCTTATTATCGCTTCCGCCATGCGGTTAAGCCTGGTGTGA
CAGGGTTAGCACAAGTCAGTTATCCTTATGGCGCTTCGGTTGAGGATGCAGTGTGGAAGCATAAATATGATATTTACTAT
ATTAAACATCATAGTACTTTATTAGATATAAAAATTCTGTTGAAAACGGTTAAAGTTGTTTTATTTGGTATGGGACGATA
A

Upstream 100 bases:

>100_bases
TGTACTAGGCACCATTTTTGGGGGGATGTTAGGTGTGATGTTTGTATTGGTAAGGCATTTTATAAGAAAAGAAGATTAGG
ATTCAATTGCGTGTATTAGA

Downstream 100 bases:

>100_bases
ATGGAATATCAACCACTAGTATCGATTATCATGCCAAGCTATAACTCAGTCAGAACTATAGCTGAAAGTATTGAGTCAGT
TATAGCTCAAACATATAAAA

Product: exopolysaccharide biosynthesis polyprenyl glycosylphosphotransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 346; Mature: 345

Protein sequence:

>346_residues
MALSLLVPEPINNLVYCMNIRVNETVDTEEFQPIKTVIKRFKSKRQVLTQEVIYVYCPKDRELEIGEIIDDYSFDMVVND
YSITDLSCKVICHFESARLTKEQRQFLVKATELGAWVEPLVSYLDERLGYTEVRLLHSGYFLHQKAFSILSTKRTQLAKR
VIDLFAALLLCTIAIPLGLLTALAIKLESPGPVFYKQRRTGQYNDEFEVIKFRSMRNDAEKSGAQWASQNDARVTKVGNF
IRKTRIDELPQIINILKGEMSIVGPRPEREVFIAELEKEIPYYRFRHAVKPGVTGLAQVSYPYGASVEDAVWKHKYDIYY
IKHHSTLLDIKILLKTVKVVLFGMGR

Sequences:

>Translated_346_residues
MALSLLVPEPINNLVYCMNIRVNETVDTEEFQPIKTVIKRFKSKRQVLTQEVIYVYCPKDRELEIGEIIDDYSFDMVVND
YSITDLSCKVICHFESARLTKEQRQFLVKATELGAWVEPLVSYLDERLGYTEVRLLHSGYFLHQKAFSILSTKRTQLAKR
VIDLFAALLLCTIAIPLGLLTALAIKLESPGPVFYKQRRTGQYNDEFEVIKFRSMRNDAEKSGAQWASQNDARVTKVGNF
IRKTRIDELPQIINILKGEMSIVGPRPEREVFIAELEKEIPYYRFRHAVKPGVTGLAQVSYPYGASVEDAVWKHKYDIYY
IKHHSTLLDIKILLKTVKVVLFGMGR
>Mature_345_residues
ALSLLVPEPINNLVYCMNIRVNETVDTEEFQPIKTVIKRFKSKRQVLTQEVIYVYCPKDRELEIGEIIDDYSFDMVVNDY
SITDLSCKVICHFESARLTKEQRQFLVKATELGAWVEPLVSYLDERLGYTEVRLLHSGYFLHQKAFSILSTKRTQLAKRV
IDLFAALLLCTIAIPLGLLTALAIKLESPGPVFYKQRRTGQYNDEFEVIKFRSMRNDAEKSGAQWASQNDARVTKVGNFI
RKTRIDELPQIINILKGEMSIVGPRPEREVFIAELEKEIPYYRFRHAVKPGVTGLAQVSYPYGASVEDAVWKHKYDIYYI
KHHSTLLDIKILLKTVKVVLFGMGR

Specific function: Slime polysaccharide colanic acid biosynthesis. [C]

COG id: COG2148

COG function: function code M; Sugar transferases involved in lipopolysaccharide synthesis

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial sugar transferase family [H]

Homologues:

Organism=Escherichia coli, GI1788360, Length=185, Percent_Identity=43.7837837837838, Blast_Score=147, Evalue=1e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003362
- InterPro:   IPR017475
- InterPro:   IPR017473 [H]

Pfam domain/function: PF02397 Bac_transf [H]

EC number: NA

Molecular weight: Translated: 39893; Mature: 39762

Theoretical pI: Translated: 8.98; Mature: 8.98

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALSLLVPEPINNLVYCMNIRVNETVDTEEFQPIKTVIKRFKSKRQVLTQEVIYVYCPKD
CEEEEECCCCCCCEEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCEEEEECCCC
RELEIGEIIDDYSFDMVVNDYSITDLSCKVICHFESARLTKEQRQFLVKATELGAWVEPL
CCEEHHHHHCCCCCEEEEECCEECCCEEEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHH
VSYLDERLGYTEVRLLHSGYFLHQKAFSILSTKRTQLAKRVIDLFAALLLCTIAIPLGLL
HHHHHHHCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TALAIKLESPGPVFYKQRRTGQYNDEFEVIKFRSMRNDAEKSGAQWASQNDARVTKVGNF
HHHHHEECCCCCCEEECCCCCCCCCCHHHEEHHHHHCHHHHHCCCCCCCCCCHHHHHHHH
IRKTRIDELPQIINILKGEMSIVGPRPEREVFIAELEKEIPYYRFRHAVKPGVTGLAQVS
HHHHHHHHHHHHHHHHCCCCEEECCCCCHHHHHHHHHHHCCHHHHHHHCCCCCCCCEEEC
YPYGASVEDAVWKHKYDIYYIKHHSTLLDIKILLKTVKVVLFGMGR
CCCCCCHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
ALSLLVPEPINNLVYCMNIRVNETVDTEEFQPIKTVIKRFKSKRQVLTQEVIYVYCPKD
EEEEECCCCCCCEEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCEEEEECCCC
RELEIGEIIDDYSFDMVVNDYSITDLSCKVICHFESARLTKEQRQFLVKATELGAWVEPL
CCEEHHHHHCCCCCEEEEECCEECCCEEEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHH
VSYLDERLGYTEVRLLHSGYFLHQKAFSILSTKRTQLAKRVIDLFAALLLCTIAIPLGLL
HHHHHHHCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TALAIKLESPGPVFYKQRRTGQYNDEFEVIKFRSMRNDAEKSGAQWASQNDARVTKVGNF
HHHHHEECCCCCCEEECCCCCCCCCCHHHEEHHHHHCHHHHHCCCCCCCCCCHHHHHHHH
IRKTRIDELPQIINILKGEMSIVGPRPEREVFIAELEKEIPYYRFRHAVKPGVTGLAQVS
HHHHHHHHHHHHHHHHCCCCEEECCCCCHHHHHHHHHHHCCHHHHHHHCCCCCCCCEEEC
YPYGASVEDAVWKHKYDIYYIKHHSTLLDIKILLKTVKVVLFGMGR
CCCCCCHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8759852; 9097040; 9278503 [H]