Click here to switch to the map view.

The map label for this gene is ptsH [H]

Identifier: 160875577

GI number: 160875577

Start: 2916358

End: 2916630

Strand: Direct

Name: ptsH [H]

Synonym: Sbal195_2465

Alternate gene names: 160875577

Gene position: 2916358-2916630 (Clockwise)

Preceding gene: 160875576

Following gene: 160875578

Centisome position: 54.54

GC content: 47.99

Gene sequence:

>273_bases
ATGAGCTCATCCACTATGTACGAAAAATCTGTCACCATTACCGCCAAACATGGCATTCACACCCGCCCTGCAGCTTTACT
GGTTAAAGAAGCCAAAACCTTTGACTGCGATGTCATGGTCGAATGCAATGGTAAGCAAGCCAGCGCTAAAAGTCTGTTTA
AGCTACAAACTTTAGGGCTATATTCTGGCGTCACGGTAAGAGTGTTTGCCGAAGGTGAACAGGCACAAGCCGCGGTTGAG
AAAGTCTCCGAGCTGCTGATCACGCTAAGCTAG

Upstream 100 bases:

>100_bases
CTCGTGAAAAATAATCGCCTCAAAAGTGCCATCGCCGCCTTGAAAAAACAGGGCTACCACATCATAGAATCGAAAGACGA
AGTGTAATACCCCATTAAAT

Downstream 100 bases:

>100_bases
GCCTTTATCCTATTTACTAGTGCACTGATTAGCGCACTAATATCTAAGTGCTTACATGTAGATCTTACCGACGAGGGATT
AGTTGAAACGGATTCTGTTC

Product: phosphotransferase system, phosphocarrier protein HPr

Products: D-sorbitol 6-phosphate [Cytoplasm]; pyruvate; alpha,alpha-trehalose 6-phosphate [Cytoplasm]; mannitol-1-phosphate [Cytoplasm]; D-glucosamine-6-phosphate [Cytoplasm]; N-acetyl-D-glucosamine-6-phosphate [Cytoplasm]; mannose-6-phosphate [Cytoplasm]; galactitol-1-phosphate [Cytoplasm]; fructose-6-phosphate [Cytoplasm]; fructose-1-phosphate [Cytoplasm]; glucose-6-phosphate [Cytoplasm]; mannitol-1-phosphate [Cytoplasm]; diacetylchitobiose-6-phosphate [Cytoplasm]; cellobiose-6-phosphate [Cytoplasm]; salicin-6-phosphate [Cytoplasm]; arbutin-6-phosphate [Cytoplasm] [C]

Alternate protein names: Histidine-containing protein [H]

Number of amino acids: Translated: 90; Mature: 89

Protein sequence:

>90_residues
MSSSTMYEKSVTITAKHGIHTRPAALLVKEAKTFDCDVMVECNGKQASAKSLFKLQTLGLYSGVTVRVFAEGEQAQAAVE
KVSELLITLS

Sequences:

>Translated_90_residues
MSSSTMYEKSVTITAKHGIHTRPAALLVKEAKTFDCDVMVECNGKQASAKSLFKLQTLGLYSGVTVRVFAEGEQAQAAVE
KVSELLITLS
>Mature_89_residues
SSSTMYEKSVTITAKHGIHTRPAALLVKEAKTFDCDVMVECNGKQASAKSLFKLQTLGLYSGVTVRVFAEGEQAQAAVEK
VSELLITLS

Specific function: General (non sugar-specific) component of the phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS). This major carbohydrate active-transport system catalyzes the phosphorylation of incoming sugar substrates concomitantly with their tr

COG id: COG1925

COG function: function code G; Phosphotransferase system, HPr-related proteins

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HPr domain [H]

Homologues:

Organism=Escherichia coli, GI1788755, Length=84, Percent_Identity=58.3333333333333, Blast_Score=98, Evalue=9e-23,

Paralogues:

None

Copy number: 4180 Molecules/Cell In: Early Stationary Phase, Rich Media (Based on E. coli). 2100 Molecules/Cell In: Early Stationary Phase, Rich Media (Based on E. coli). 3235 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001020
- InterPro:   IPR005698
- InterPro:   IPR000032
- InterPro:   IPR002114 [H]

Pfam domain/function: PF00381 PTS-HPr [H]

EC number: NA

Molecular weight: Translated: 9694; Mature: 9563

Theoretical pI: Translated: 8.23; Mature: 8.23

Prosite motif: PS00369 PTS_HPR_HIS ; PS00589 PTS_HPR_SER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSSTMYEKSVTITAKHGIHTRPAALLVKEAKTFDCDVMVECNGKQASAKSLFKLQTLGL
CCCCCCEEEEEEEEECCCCCCCCHHEEEECCCCCCCEEEEEECCCCCCHHHHHHHHCCCC
YSGVTVRVFAEGEQAQAAVEKVSELLITLS
CCCCEEEEEECCCHHHHHHHHHHHHHEECC
>Mature Secondary Structure 
SSSTMYEKSVTITAKHGIHTRPAALLVKEAKTFDCDVMVECNGKQASAKSLFKLQTLGL
CCCCCEEEEEEEEECCCCCCCCHHEEEECCCCCCCEEEEEECCCCCCHHHHHHHHCCCC
YSGVTVRVFAEGEQAQAAVEKVSELLITLS
CCCCEEEEEECCCHHHHHHHHHHHHHEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: sorbitol [Periplasm]; phosphoenolpyruvate; trehalose [Periplasm]; mannitol [Periplasm]; glucosamine [Periplasm]; N-acetyl-D-glucosamine [Periplasm]; mannose [Periplasm]; galactitol [Periplasm]; fructose [Periplasm]; beta-D-glucose [Periplasm]; diacetylchitobiose [Periplasm]; cellobiose [Periplasm]; salicin [Periplasm]; arbutin [Periplasm] [C]

Specific reaction: phosphoenolpyruvate + sorbitol [Periplasm] = D-sorbitol 6-phosphate [Cytoplasm] + pyruvate phosphoenolpyruvate + trehalose [Periplasm] = alpha,alpha-trehalose 6-phosphate [Cytoplasm] + pyruvate phosphoenolpyruvate + mannitol [Periplasm] = mannitol-1-phosp

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]