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The map label for this gene is ptsI [H]

Identifier: 160875578

GI number: 160875578

Start: 2916834

End: 2918537

Strand: Direct

Name: ptsI [H]

Synonym: Sbal195_2466

Alternate gene names: 160875578

Gene position: 2916834-2918537 (Clockwise)

Preceding gene: 160875577

Following gene: 160875579

Centisome position: 54.55

GC content: 49.53

Gene sequence:

>1704_bases
ATGTCAATAACGGGGATTATAGTGTCATCGGGAATTGCCTTTGGTCAGGCACTTCACCTTATTCACACCGAACACCACCT
CGATTACCGCCCTATTCCCCTGTCGAAAATTCCCCAACAACAGGGCAAGTTTGCCAAAGCCTTGCAAGAGCTAAAGGCAC
AACTGGCCCACAGCCAAGCCGCACTCGATAGCGACTCGGAAATTTATCAGCTGATCGAAGCCGACCTATTGTTATTGGAA
GATGATGAATTAATCGAGCAAGTGAACGATGCGATTCGTACCTTACAACTGTCGGCAAGTGTGGCGGTTGAACGCATATT
TGCTCATCAAGCCAACGAGCTGCAATCCCTAGATGATCCCTATTTAGCTAATCGAGCCCAAGATGTACGCTGTTTAGGCC
AACGCGTTGTCGCCGCGATCAATGGCCATTTAAACCAAGGGCTCGAAAAACTCGACAGACCCACCATCTTGTTGGCGCAA
GATTTAACCCCCGCCGAATTTGCCTTATTGCCGAGGGAAAACCTCTGCGGTATTGTGCTCAAAACTGGCGGTTTAACCAG
TCATACGGCGATTTTAGCCCGAGCTGCCGGCATTCCAGCCATCTTAAGTTGTCAGTTTGATGCCGATTCGATCCCCAACG
GCACGCCCTTAGTACTCGATGCGCTCAATGGTGAGCTTTGCGTTAATCCTAATCCTGATCAACAGGCAAGACTTACCGTC
ACCTTTCACCACGAACAGGCAAGACGGGCAGCGCTGCAAACCTATAAGGATGGCCCAGCGCAAACCCAAGATGGCCATAT
CGTAGGGCTTATGGCCAACGTGGGTAATCTCAACGACATCACCCATGTCAGCGATGTTGGCGCCGATGGTATAGGTTTGT
TTCGCACTGAATTTATGTTGATGAACGTCAGCACCCTGCCCGATGAGAAAGCCCAATACAGCTTATATTGCGATGCATTG
CACGCTCTGGGCGGTAAAACTTTTACCATCCGCACCTTAGATATCGGTGCTGACAAAGAACTGCCTTGCCTGTGCCAAGA
AATAGAAGATAATCCCGCCTTAGGGCTGCGCGGTATTCGCTATACCTTGGCACACCCCGACTTATTTAAAACCCAATTGA
GGGCTATTTTGCGCGCCGCAAACCACGGTCCGATCCGCTTGATGTTCCCTATGGTCAATCAAGTCGAAGAATTGGATGAA
GTGTTTGCACTGATTGCCCAGTGCCAGGATGCCCTGGAAGAAGAAGAGAAAGGCTACGGTGAACTCAGCTACGGTATCGT
TGTCGAAACCCCCGCAGCGGTATTTAACCTCAATGCTATGCTGCCACGACTCGACTTTGTCAGCATTGGCACCAATGATT
TAACCCAATATGCAATGGCAGCCGATAGGACAAACCCGCAGCTTACCCGCGACTATCCGTCCCTTTCGCCTGCCATTTTA
GCGTTAATTAACATGACAATAGTCCAAGCAAAAGCGGCCAATGTGAAAGTGTCGCTGTGCGGCGAACTCGCCAGTTCACC
ACAAATTGCACCGCTGTTAATCGGCATGGGGCTGGACGAACTCAGTGTTAACTTAAGCTCACTGTTAGAAGTCAAAGCTG
CCATTTGCCAAGGCAACATCCAACAATTTTCGGCGCTGGCGCACACAGCATTACAACAAGATAGAATTGCAGGTCTACAG
CAGTGTATAACAAGCTATAAATAG

Upstream 100 bases:

>100_bases
CCAATAGTTTACATGCCAACGCTTTACAAACTCAACGCCACTCAACTAGACTCGCTAGCGCTAACGGGCACTCAGTTCAG
TGGTTAATTTAGGAAGGGAT

Downstream 100 bases:

>100_bases
AGACATTTATTCGTTATGCTGCATTACAGAACAAAAACAATGACAAATACAAGCAAAAGAGGCTCTAGATTATGGGATTT
TTAAGCCGAATAAGGCGCTT

Product: phosphoenolpyruvate-protein phosphotransferase

Products: NA

Alternate protein names: Phosphotransferase system, enzyme I [H]

Number of amino acids: Translated: 567; Mature: 566

Protein sequence:

>567_residues
MSITGIIVSSGIAFGQALHLIHTEHHLDYRPIPLSKIPQQQGKFAKALQELKAQLAHSQAALDSDSEIYQLIEADLLLLE
DDELIEQVNDAIRTLQLSASVAVERIFAHQANELQSLDDPYLANRAQDVRCLGQRVVAAINGHLNQGLEKLDRPTILLAQ
DLTPAEFALLPRENLCGIVLKTGGLTSHTAILARAAGIPAILSCQFDADSIPNGTPLVLDALNGELCVNPNPDQQARLTV
TFHHEQARRAALQTYKDGPAQTQDGHIVGLMANVGNLNDITHVSDVGADGIGLFRTEFMLMNVSTLPDEKAQYSLYCDAL
HALGGKTFTIRTLDIGADKELPCLCQEIEDNPALGLRGIRYTLAHPDLFKTQLRAILRAANHGPIRLMFPMVNQVEELDE
VFALIAQCQDALEEEEKGYGELSYGIVVETPAAVFNLNAMLPRLDFVSIGTNDLTQYAMAADRTNPQLTRDYPSLSPAIL
ALINMTIVQAKAANVKVSLCGELASSPQIAPLLIGMGLDELSVNLSSLLEVKAAICQGNIQQFSALAHTALQQDRIAGLQ
QCITSYK

Sequences:

>Translated_567_residues
MSITGIIVSSGIAFGQALHLIHTEHHLDYRPIPLSKIPQQQGKFAKALQELKAQLAHSQAALDSDSEIYQLIEADLLLLE
DDELIEQVNDAIRTLQLSASVAVERIFAHQANELQSLDDPYLANRAQDVRCLGQRVVAAINGHLNQGLEKLDRPTILLAQ
DLTPAEFALLPRENLCGIVLKTGGLTSHTAILARAAGIPAILSCQFDADSIPNGTPLVLDALNGELCVNPNPDQQARLTV
TFHHEQARRAALQTYKDGPAQTQDGHIVGLMANVGNLNDITHVSDVGADGIGLFRTEFMLMNVSTLPDEKAQYSLYCDAL
HALGGKTFTIRTLDIGADKELPCLCQEIEDNPALGLRGIRYTLAHPDLFKTQLRAILRAANHGPIRLMFPMVNQVEELDE
VFALIAQCQDALEEEEKGYGELSYGIVVETPAAVFNLNAMLPRLDFVSIGTNDLTQYAMAADRTNPQLTRDYPSLSPAIL
ALINMTIVQAKAANVKVSLCGELASSPQIAPLLIGMGLDELSVNLSSLLEVKAAICQGNIQQFSALAHTALQQDRIAGLQ
QCITSYK
>Mature_566_residues
SITGIIVSSGIAFGQALHLIHTEHHLDYRPIPLSKIPQQQGKFAKALQELKAQLAHSQAALDSDSEIYQLIEADLLLLED
DELIEQVNDAIRTLQLSASVAVERIFAHQANELQSLDDPYLANRAQDVRCLGQRVVAAINGHLNQGLEKLDRPTILLAQD
LTPAEFALLPRENLCGIVLKTGGLTSHTAILARAAGIPAILSCQFDADSIPNGTPLVLDALNGELCVNPNPDQQARLTVT
FHHEQARRAALQTYKDGPAQTQDGHIVGLMANVGNLNDITHVSDVGADGIGLFRTEFMLMNVSTLPDEKAQYSLYCDALH
ALGGKTFTIRTLDIGADKELPCLCQEIEDNPALGLRGIRYTLAHPDLFKTQLRAILRAANHGPIRLMFPMVNQVEELDEV
FALIAQCQDALEEEEKGYGELSYGIVVETPAAVFNLNAMLPRLDFVSIGTNDLTQYAMAADRTNPQLTRDYPSLSPAILA
LINMTIVQAKAANVKVSLCGELASSPQIAPLLIGMGLDELSVNLSSLLEVKAAICQGNIQQFSALAHTALQQDRIAGLQQ
CITSYK

Specific function: General (non sugar-specific) component of the phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS). This major carbohydrate active-transport system catalyzes the phosphorylation of incoming sugar substrates concomitantly with their tr

COG id: COG1080

COG function: function code G; Phosphoenolpyruvate-protein kinase (PTS system EI component in bacteria)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PEP-utilizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1788756, Length=554, Percent_Identity=37.5451263537906, Blast_Score=346, Evalue=2e-96,
Organism=Escherichia coli, GI1789193, Length=461, Percent_Identity=36.8763557483731, Blast_Score=266, Evalue=2e-72,
Organism=Escherichia coli, GI1788726, Length=569, Percent_Identity=32.688927943761, Blast_Score=265, Evalue=6e-72,
Organism=Escherichia coli, GI48994992, Length=497, Percent_Identity=33.8028169014084, Blast_Score=257, Evalue=1e-69,
Organism=Escherichia coli, GI1787994, Length=451, Percent_Identity=23.2815964523282, Blast_Score=93, Evalue=5e-20,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2659 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008279
- InterPro:   IPR006318
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR008731
- InterPro:   IPR015813 [H]

Pfam domain/function: PF05524 PEP-utilisers_N; PF00391 PEP-utilizers; PF02896 PEP-utilizers_C [H]

EC number: =2.7.3.9 [H]

Molecular weight: Translated: 61528; Mature: 61397

Theoretical pI: Translated: 4.69; Mature: 4.69

Prosite motif: PS00370 PEP_ENZYMES_PHOS_SITE ; PS00742 PEP_ENZYMES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSITGIIVSSGIAFGQALHLIHTEHHLDYRPIPLSKIPQQQGKFAKALQELKAQLAHSQA
CCEEEEEEECCHHHHHHHHHHHHHCCCCCCCCCHHHCCHHCCHHHHHHHHHHHHHHHHHH
ALDSDSEIYQLIEADLLLLEDDELIEQVNDAIRTLQLSASVAVERIFAHQANELQSLDDP
HCCCCHHHHHHHHHCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
YLANRAQDVRCLGQRVVAAINGHLNQGLEKLDRPTILLAQDLTPAEFALLPRENLCGIVL
HHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHEEECCCCCCCEEEE
KTGGLTSHTAILARAAGIPAILSCQFDADSIPNGTPLVLDALNGELCVNPNPDQQARLTV
ECCCCCHHHHHHHHHCCCCEEEEEECCCCCCCCCCCEEEEECCCCEEECCCCCCCEEEEE
TFHHEQARRAALQTYKDGPAQTQDGHIVGLMANVGNLNDITHVSDVGADGIGLFRTEFML
EEEHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCCCCCHHHHCCCCCCHHHHHHEEE
MNVSTLPDEKAQYSLYCDALHALGGKTFTIRTLDIGADKELPCLCQEIEDNPALGLRGIR
EECCCCCCCCCHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCHHHHHCCCCCCCCCCCEE
YTLAHPDLFKTQLRAILRAANHGPIRLMFPMVNQVEELDEVFALIAQCQDALEEEEKGYG
EEECCCHHHHHHHHHHHHHCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
ELSYGIVVETPAAVFNLNAMLPRLDFVSIGTNDLTQYAMAADRTNPQLTRDYPSLSPAIL
EEEECEEEECCHHHHEHHHHCCCCEEEECCCHHHHHHHHHHCCCCCCCCCCCCCCCHHHH
ALINMTIVQAKAANVKVSLCGELASSPQIAPLLIGMGLDELSVNLSSLLEVKAAICQGNI
HHHHHHHHEECCCCEEEEEHHHHCCCCCCCHHEEECCHHHHHHHHHHHHHHHHHHHCCCH
QQFSALAHTALQQDRIAGLQQCITSYK
HHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SITGIIVSSGIAFGQALHLIHTEHHLDYRPIPLSKIPQQQGKFAKALQELKAQLAHSQA
CEEEEEEECCHHHHHHHHHHHHHCCCCCCCCCHHHCCHHCCHHHHHHHHHHHHHHHHHH
ALDSDSEIYQLIEADLLLLEDDELIEQVNDAIRTLQLSASVAVERIFAHQANELQSLDDP
HCCCCHHHHHHHHHCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
YLANRAQDVRCLGQRVVAAINGHLNQGLEKLDRPTILLAQDLTPAEFALLPRENLCGIVL
HHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHEEECCCCCCCEEEE
KTGGLTSHTAILARAAGIPAILSCQFDADSIPNGTPLVLDALNGELCVNPNPDQQARLTV
ECCCCCHHHHHHHHHCCCCEEEEEECCCCCCCCCCCEEEEECCCCEEECCCCCCCEEEEE
TFHHEQARRAALQTYKDGPAQTQDGHIVGLMANVGNLNDITHVSDVGADGIGLFRTEFML
EEEHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCCCCCHHHHCCCCCCHHHHHHEEE
MNVSTLPDEKAQYSLYCDALHALGGKTFTIRTLDIGADKELPCLCQEIEDNPALGLRGIR
EECCCCCCCCCHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCHHHHHCCCCCCCCCCCEE
YTLAHPDLFKTQLRAILRAANHGPIRLMFPMVNQVEELDEVFALIAQCQDALEEEEKGYG
EEECCCHHHHHHHHHHHHHCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
ELSYGIVVETPAAVFNLNAMLPRLDFVSIGTNDLTQYAMAADRTNPQLTRDYPSLSPAIL
EEEECEEEECCHHHHEHHHHCCCCEEEECCCHHHHHHHHHHCCCCCCCCCCCCCCCHHHH
ALINMTIVQAKAANVKVSLCGELASSPQIAPLLIGMGLDELSVNLSSLLEVKAAICQGNI
HHHHHHHHEECCCCEEEEEHHHHCCCCCCCHHEEECCHHHHHHHHHHHHHHHHHHHCCCH
QQFSALAHTALQQDRIAGLQQCITSYK
HHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]