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The map label for this gene is sppA [H]

Identifier: 160875523

GI number: 160875523

Start: 2848228

End: 2850075

Strand: Direct

Name: sppA [H]

Synonym: Sbal195_2411

Alternate gene names: 160875523

Gene position: 2848228-2850075 (Clockwise)

Preceding gene: 160875521

Following gene: 160875524

Centisome position: 53.26

GC content: 48.48

Gene sequence:

>1848_bases
ATGTCCGCTAACCCATCGTTTTTCAAGAGAATATGTTTGTTTATTTGGCACACCCTCAACGGGACGCGTAAACTGATCCT
CAATCTGATCTTTTTTGGCATCTTAGCCACCATCATTATTTCTATTGGCAGCAGCGAAGATATCCAAGTAGAAGACAACT
CGGCGCTGGTGCTTAATCTGGCCGGCTCGATTGTCGATCAGAAACAACAGGTCGATCCCTTAGAAGCCGCATTCAAGCAA
GGCCAAAATGCCAACGCAGATGGGGAAATCCTGCTCGCCGATGTGCTGTATGTGATTGACAATGCCGCGCAAGATCAAAG
GATTAGTACCCTAGTACTTGATCTAGCCGATTTGAAACGTGCAGGGATCAGCAAGCTGCAATCTATCGGTGATGCACTTA
ACCGCTTTAAAGAAAGCGGTAAAAAAGTGGTCGCCATCGGTAATTACTACGAGCAGAATCAATATTTCCTCGCCAGTTTT
GCCGACACCATTTACCTCAATCCCCAAGGCGGCGTGTCGCTTGATGGCCTGAGTATGTACAACCAGTACTTCAAATCGGC
CTTAGATAAACTCAAAGTTAAAGCGCATATTTTCCGCGTAGGGACTTTTAAATCTGCGGTTGAACCCTACATGCGTGACG
ATATGTCCGACGCCGCTCGCGAAGCCAGCAGTGCGCTTTTAGCGGATGTATGGCAAAGCTACACCCAGACTGTCGCAGGC
AATCGCAATATTGAGCCTAACTCGCTGGTGCCCGATGCAACCACTTATTTGGCCGAGCTCGATAAAGCCAATGGTGACTC
TGCGGCGATGGCCATCAACATGAAATGGGTTGATAGCTTAGCGACCACGGAAGATTTTCGTCAAACCATGTTAGAAACAG
TGGGCAAGGCCAGCAGCGGTGACAGTTTCAAGCAAGTGAGTTTTTATGATTATTTAACCTTAGTGACGCCATTGCCAAGC
TTTGTTGAGCAAGACAGTGTCGGCATAATTGTGGCGAGCGGTACGATTCTGAACGGCACTCAACCTGCTGGCCAAATTGG
CGGCGAAAGCACTGCCGAGCTGTTACGTAAGGCCCGTTTTGATAAACACGTTAAAGCCTTAGTGCTGCGCGTCGATAGCC
CTGGCGGTAGTGCCTTTGCCTCGGAGCAAATTCGTCAAGAATTGCTGGCACTTAAAGCGGCTGGCAAACCTGTTGTCGTC
AGTATGGGCAGTTTAGCCGCATCGGGTGGTTATTGGATTTCAGCCAGTGCCGACTATATCTTCGCAACGCCAACCACGCT
CACGGGCTCAATTGGGATCTTCGGCATGATCACCACCTTCGAAGATTCTCTGGCCAGTATAGGCGTGCATACCGATGGCG
TATCAACATCGGAATGGGCGGGATTGTCTGTGACGCGCACGCTGTCACCGCAAGTTGAAGCGATTATCCAACGTCATATT
GAACGTGGCTATTTAGACTTTATCTCGCTGGTCGCTAAAGAACGAAAGATGACCATTGAACAAGTCGATAAGATTGCCCA
AGGCCGTGTTTGGAGTGGTAAAAAAGCCCTTGAGCTTGGCTTAGTCGATGAACTGGGTGATTTAGATGAGGCGATAGCTA
AAGCCGCTAAACTCGCGGATATGACGCTATTTGATACCCGCGTTATCGAACAAGAACTGACACCCGAGCAACGCTTTATG
CAACAGATGTTTGCCTCAGTATCGAGCTACCTGCCCGCCTCACTGAGCCAATCATCGATGTTAGAGCAAATGCTAGCGCA
ATTGACCAGCAGCTTGAAAACGTTAACCTCTTTTGATGATCCAAATAACGTCTACATCTACTGCGATACTTGCAACATGA
TGAACTAA

Upstream 100 bases:

>100_bases
TTGGATCGAGGCCATCAGTCAAAATCCTTTACAATTGTGTTTAGCCTGTAGCGCCATTTTCAGTTAGCATTGGCAGATTG
GACTTATAAAGGAGTGGCAG

Downstream 100 bases:

>100_bases
AGCTGCCAGCGTCTAGGTTGCTGCTCGCAACTTCAAGATGATGACAAAGACAAAGCCCGGATCTCCGGGCTTTTTTATTG
GGTTTATGTCAGTTAAATCG

Product: signal peptide peptidase SppA

Products: NA

Alternate protein names: Endopeptidase IV; Protease IV; Signal peptide peptidase [H]

Number of amino acids: Translated: 615; Mature: 614

Protein sequence:

>615_residues
MSANPSFFKRICLFIWHTLNGTRKLILNLIFFGILATIIISIGSSEDIQVEDNSALVLNLAGSIVDQKQQVDPLEAAFKQ
GQNANADGEILLADVLYVIDNAAQDQRISTLVLDLADLKRAGISKLQSIGDALNRFKESGKKVVAIGNYYEQNQYFLASF
ADTIYLNPQGGVSLDGLSMYNQYFKSALDKLKVKAHIFRVGTFKSAVEPYMRDDMSDAAREASSALLADVWQSYTQTVAG
NRNIEPNSLVPDATTYLAELDKANGDSAAMAINMKWVDSLATTEDFRQTMLETVGKASSGDSFKQVSFYDYLTLVTPLPS
FVEQDSVGIIVASGTILNGTQPAGQIGGESTAELLRKARFDKHVKALVLRVDSPGGSAFASEQIRQELLALKAAGKPVVV
SMGSLAASGGYWISASADYIFATPTTLTGSIGIFGMITTFEDSLASIGVHTDGVSTSEWAGLSVTRTLSPQVEAIIQRHI
ERGYLDFISLVAKERKMTIEQVDKIAQGRVWSGKKALELGLVDELGDLDEAIAKAAKLADMTLFDTRVIEQELTPEQRFM
QQMFASVSSYLPASLSQSSMLEQMLAQLTSSLKTLTSFDDPNNVYIYCDTCNMMN

Sequences:

>Translated_615_residues
MSANPSFFKRICLFIWHTLNGTRKLILNLIFFGILATIIISIGSSEDIQVEDNSALVLNLAGSIVDQKQQVDPLEAAFKQ
GQNANADGEILLADVLYVIDNAAQDQRISTLVLDLADLKRAGISKLQSIGDALNRFKESGKKVVAIGNYYEQNQYFLASF
ADTIYLNPQGGVSLDGLSMYNQYFKSALDKLKVKAHIFRVGTFKSAVEPYMRDDMSDAAREASSALLADVWQSYTQTVAG
NRNIEPNSLVPDATTYLAELDKANGDSAAMAINMKWVDSLATTEDFRQTMLETVGKASSGDSFKQVSFYDYLTLVTPLPS
FVEQDSVGIIVASGTILNGTQPAGQIGGESTAELLRKARFDKHVKALVLRVDSPGGSAFASEQIRQELLALKAAGKPVVV
SMGSLAASGGYWISASADYIFATPTTLTGSIGIFGMITTFEDSLASIGVHTDGVSTSEWAGLSVTRTLSPQVEAIIQRHI
ERGYLDFISLVAKERKMTIEQVDKIAQGRVWSGKKALELGLVDELGDLDEAIAKAAKLADMTLFDTRVIEQELTPEQRFM
QQMFASVSSYLPASLSQSSMLEQMLAQLTSSLKTLTSFDDPNNVYIYCDTCNMMN
>Mature_614_residues
SANPSFFKRICLFIWHTLNGTRKLILNLIFFGILATIIISIGSSEDIQVEDNSALVLNLAGSIVDQKQQVDPLEAAFKQG
QNANADGEILLADVLYVIDNAAQDQRISTLVLDLADLKRAGISKLQSIGDALNRFKESGKKVVAIGNYYEQNQYFLASFA
DTIYLNPQGGVSLDGLSMYNQYFKSALDKLKVKAHIFRVGTFKSAVEPYMRDDMSDAAREASSALLADVWQSYTQTVAGN
RNIEPNSLVPDATTYLAELDKANGDSAAMAINMKWVDSLATTEDFRQTMLETVGKASSGDSFKQVSFYDYLTLVTPLPSF
VEQDSVGIIVASGTILNGTQPAGQIGGESTAELLRKARFDKHVKALVLRVDSPGGSAFASEQIRQELLALKAAGKPVVVS
MGSLAASGGYWISASADYIFATPTTLTGSIGIFGMITTFEDSLASIGVHTDGVSTSEWAGLSVTRTLSPQVEAIIQRHIE
RGYLDFISLVAKERKMTIEQVDKIAQGRVWSGKKALELGLVDELGDLDEAIAKAAKLADMTLFDTRVIEQELTPEQRFMQ
QMFASVSSYLPASLSQSSMLEQMLAQLTSSLKTLTSFDDPNNVYIYCDTCNMMN

Specific function: Digestion of the cleaved signal peptides. This activity is necessary to maintain proper secretion of mature proteins across the membrane [H]

COG id: COG0616

COG function: function code OU; Periplasmic serine proteases (ClpP class)

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S49 family [H]

Homologues:

Organism=Escherichia coli, GI1788064, Length=617, Percent_Identity=45.7050243111831, Blast_Score=504, Evalue=1e-144,
Organism=Escherichia coli, GI1787527, Length=171, Percent_Identity=31.5789473684211, Blast_Score=73, Evalue=5e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004634
- InterPro:   IPR004635
- InterPro:   IPR002142 [H]

Pfam domain/function: PF01343 Peptidase_S49 [H]

EC number: 3.4.21.-

Molecular weight: Translated: 66946; Mature: 66815

Theoretical pI: Translated: 4.60; Mature: 4.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSANPSFFKRICLFIWHTLNGTRKLILNLIFFGILATIIISIGSSEDIQVEDNSALVLNL
CCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCEEEEEE
AGSIVDQKQQVDPLEAAFKQGQNANADGEILLADVLYVIDNAAQDQRISTLVLDLADLKR
CHHHHHHHHCCCHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
AGISKLQSIGDALNRFKESGKKVVAIGNYYEQNQYFLASFADTIYLNPQGGVSLDGLSMY
HHHHHHHHHHHHHHHHHHCCCEEEEECCEECCCCEEEEEECCEEEECCCCCCCCCHHHHH
NQYFKSALDKLKVKAHIFRVGTFKSAVEPYMRDDMSDAAREASSALLADVWQSYTQTVAG
HHHHHHHHHHHHHHHHEEEECCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCC
NRNIEPNSLVPDATTYLAELDKANGDSAAMAINMKWVDSLATTEDFRQTMLETVGKASSG
CCCCCCCCCCCHHHHHHHHHHCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHCCCCCC
DSFKQVSFYDYLTLVTPLPSFVEQDSVGIIVASGTILNGTQPAGQIGGESTAELLRKARF
CCCHHHHHHHHHHHHHCCHHHHCCCCCEEEEECCEEECCCCCCCCCCCHHHHHHHHHHHH
DKHVKALVLRVDSPGGSAFASEQIRQELLALKAAGKPVVVSMGSLAASGGYWISASADYI
HHHHEEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCEEEECCCEECCCCEEEECCCCEE
FATPTTLTGSIGIFGMITTFEDSLASIGVHTDGVSTSEWAGLSVTRTLSPQVEAIIQRHI
EECCCCCCCCCHHHEEHHHHHHHHHHCCCCCCCCCCCCCCCCEEEECCCHHHHHHHHHHH
ERGYLDFISLVAKERKMTIEQVDKIAQGRVWSGKKALELGLVDELGDLDEAIAKAAKLAD
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHCCHHHHCHHHHHHHHHHHHHH
MTLFDTRVIEQELTPEQRFMQQMFASVSSYLPASLSQSSMLEQMLAQLTSSLKTLTSFDD
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCC
PNNVYIYCDTCNMMN
CCCEEEEEECCCCCC
>Mature Secondary Structure 
SANPSFFKRICLFIWHTLNGTRKLILNLIFFGILATIIISIGSSEDIQVEDNSALVLNL
CCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCEEEEEE
AGSIVDQKQQVDPLEAAFKQGQNANADGEILLADVLYVIDNAAQDQRISTLVLDLADLKR
CHHHHHHHHCCCHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
AGISKLQSIGDALNRFKESGKKVVAIGNYYEQNQYFLASFADTIYLNPQGGVSLDGLSMY
HHHHHHHHHHHHHHHHHHCCCEEEEECCEECCCCEEEEEECCEEEECCCCCCCCCHHHHH
NQYFKSALDKLKVKAHIFRVGTFKSAVEPYMRDDMSDAAREASSALLADVWQSYTQTVAG
HHHHHHHHHHHHHHHHEEEECCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCC
NRNIEPNSLVPDATTYLAELDKANGDSAAMAINMKWVDSLATTEDFRQTMLETVGKASSG
CCCCCCCCCCCHHHHHHHHHHCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHCCCCCC
DSFKQVSFYDYLTLVTPLPSFVEQDSVGIIVASGTILNGTQPAGQIGGESTAELLRKARF
CCCHHHHHHHHHHHHHCCHHHHCCCCCEEEEECCEEECCCCCCCCCCCHHHHHHHHHHHH
DKHVKALVLRVDSPGGSAFASEQIRQELLALKAAGKPVVVSMGSLAASGGYWISASADYI
HHHHEEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCEEEECCCEECCCCEEEECCCCEE
FATPTTLTGSIGIFGMITTFEDSLASIGVHTDGVSTSEWAGLSVTRTLSPQVEAIIQRHI
EECCCCCCCCCHHHEEHHHHHHHHHHCCCCCCCCCCCCCCCCEEEECCCHHHHHHHHHHH
ERGYLDFISLVAKERKMTIEQVDKIAQGRVWSGKKALELGLVDELGDLDEAIAKAAKLAD
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHCCHHHHCHHHHHHHHHHHHHH
MTLFDTRVIEQELTPEQRFMQQMFASVSSYLPASLSQSSMLEQMLAQLTSSLKTLTSFDD
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCC
PNNVYIYCDTCNMMN
CCCEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on peptide bonds (Peptidases) [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]