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The map label for this gene is ansA [H]

Identifier: 160875524

GI number: 160875524

Start: 2850213

End: 2851226

Strand: Direct

Name: ansA [H]

Synonym: Sbal195_2412

Alternate gene names: 160875524

Gene position: 2850213-2851226 (Clockwise)

Preceding gene: 160875523

Following gene: 160875529

Centisome position: 53.3

GC content: 49.01

Gene sequence:

>1014_bases
ATGACTAAACGCTCCATTTATGTTGCCTATACTGGCGGCACCATAGGTATGCAAAAAACCGATAACGGCTTCGTCCCTGT
GGCGGGATTCCTGACTCAGTGCGTGCAATCTATGCCTGAGTTTTACCATGAAGAAATGCCGACATTCGTGATCCACGAAT
ACTGCCCGCTTATCGACTCTTCCAATATGGCACCGACCGATTGGCAGATGATCGCCGACGATATACAAGCTAATTACGAT
AAATACGATGGCTTTGTGATCCTCCATGGCACTGACACTATGGCGTACACGGCTTCGGCGCTGTCGTTTATGCTGCAAGG
CTTGTCAAAACCTGTAATAGTGACAGGTTCGCAAATCCCCCTCGCCCAGCTGCGCTCAGACGGTCAAACCAATTTATTAA
ACTCACTCTATATTGCAGCGAATTATCCGGTCGCAGAAGTGTGTTTATTCTTCAACAACAAACTCTTTCGAGGTAATCGC
TCGACTAAGGCACACGCCGATGGCTTCGATGCTTTTGCTTCGCCTAACTTCCCGCTACTGCTAGAAGCTGGGATCAAAAT
CAATCTGCGCGCAGGCAAGATCACCGTAGCCAACGATACCCCATTGGAAGTGGCGCATATCAGTCCGCAGCCTGTGGGTG
TGGTGACCTTGTATCCAGGGATCTCGACACAGATTTTTGAGAACATTCTGCAGCAGCCGGTTAAAGCCCTCATACTATTG
ACATTTGGTGTGGGTAACGCGCCGCAAGATGCCGCTTTACTCAAAACCTTAAGGCAAGCGGATGAACGTGGCATAGTGCT
GGTGAATCTTACCCAGTGCTTCCAAGGTAAGGTGAATATGGGCGGCTACGCCACGGGCAATGCACTCGCTAAAGCGGGCG
TGATCAGTGGCGCCGATATGACCATAGAAGCGGCCTTGGCAAAACTGCATTATTTACTATCGAAAAATTTGCAGCCAGCG
GAGATAAAAGCAGCCATGTTACAAAACTTGGTCGGTGAATTGAGCATAGATTAA

Upstream 100 bases:

>100_bases
ATGATGACAAAGACAAAGCCCGGATCTCCGGGCTTTTTTATTGGGTTTATGTCAGTTAAATCGTATAATTCGGCCACTTG
AAGCTAAGAAAACGACAAGA

Downstream 100 bases:

>100_bases
AGATAGTGTCTTTTAATCTGTTCGTAGTTCTCAATCACTAGCAACAAAAAAGCCCATATTTAATGGGCTTTTTTATTTGA
ACTCAGATTAAACGACGTCA

Product: cytoplasmic asparaginase I

Products: NA

Alternate protein names: L-asparaginase I; L-ASNase I; L-asparagine amidohydrolase I [H]

Number of amino acids: Translated: 337; Mature: 336

Protein sequence:

>337_residues
MTKRSIYVAYTGGTIGMQKTDNGFVPVAGFLTQCVQSMPEFYHEEMPTFVIHEYCPLIDSSNMAPTDWQMIADDIQANYD
KYDGFVILHGTDTMAYTASALSFMLQGLSKPVIVTGSQIPLAQLRSDGQTNLLNSLYIAANYPVAEVCLFFNNKLFRGNR
STKAHADGFDAFASPNFPLLLEAGIKINLRAGKITVANDTPLEVAHISPQPVGVVTLYPGISTQIFENILQQPVKALILL
TFGVGNAPQDAALLKTLRQADERGIVLVNLTQCFQGKVNMGGYATGNALAKAGVISGADMTIEAALAKLHYLLSKNLQPA
EIKAAMLQNLVGELSID

Sequences:

>Translated_337_residues
MTKRSIYVAYTGGTIGMQKTDNGFVPVAGFLTQCVQSMPEFYHEEMPTFVIHEYCPLIDSSNMAPTDWQMIADDIQANYD
KYDGFVILHGTDTMAYTASALSFMLQGLSKPVIVTGSQIPLAQLRSDGQTNLLNSLYIAANYPVAEVCLFFNNKLFRGNR
STKAHADGFDAFASPNFPLLLEAGIKINLRAGKITVANDTPLEVAHISPQPVGVVTLYPGISTQIFENILQQPVKALILL
TFGVGNAPQDAALLKTLRQADERGIVLVNLTQCFQGKVNMGGYATGNALAKAGVISGADMTIEAALAKLHYLLSKNLQPA
EIKAAMLQNLVGELSID
>Mature_336_residues
TKRSIYVAYTGGTIGMQKTDNGFVPVAGFLTQCVQSMPEFYHEEMPTFVIHEYCPLIDSSNMAPTDWQMIADDIQANYDK
YDGFVILHGTDTMAYTASALSFMLQGLSKPVIVTGSQIPLAQLRSDGQTNLLNSLYIAANYPVAEVCLFFNNKLFRGNRS
TKAHADGFDAFASPNFPLLLEAGIKINLRAGKITVANDTPLEVAHISPQPVGVVTLYPGISTQIFENILQQPVKALILLT
FGVGNAPQDAALLKTLRQADERGIVLVNLTQCFQGKVNMGGYATGNALAKAGVISGADMTIEAALAKLHYLLSKNLQPAE
IKAAMLQNLVGELSID

Specific function: Unknown

COG id: COG0252

COG function: function code EJ; L-asparaginase/archaeal Glu-tRNAGln amidotransferase subunit D

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the asparaginase 1 family [H]

Homologues:

Organism=Homo sapiens, GI122937331, Length=356, Percent_Identity=43.2584269662921, Blast_Score=258, Evalue=6e-69,
Organism=Escherichia coli, GI1788065, Length=337, Percent_Identity=68.5459940652819, Blast_Score=484, Evalue=1e-138,
Organism=Escherichia coli, GI1789327, Length=277, Percent_Identity=28.8808664259928, Blast_Score=88, Evalue=7e-19,
Organism=Caenorhabditis elegans, GI193207372, Length=363, Percent_Identity=42.6997245179063, Blast_Score=281, Evalue=4e-76,
Organism=Caenorhabditis elegans, GI17558280, Length=363, Percent_Identity=42.6997245179063, Blast_Score=280, Evalue=6e-76,
Organism=Caenorhabditis elegans, GI193207374, Length=363, Percent_Identity=42.6997245179063, Blast_Score=280, Evalue=6e-76,
Organism=Caenorhabditis elegans, GI17558278, Length=363, Percent_Identity=42.6997245179063, Blast_Score=280, Evalue=7e-76,
Organism=Drosophila melanogaster, GI24639609, Length=359, Percent_Identity=42.3398328690808, Blast_Score=273, Evalue=9e-74,
Organism=Drosophila melanogaster, GI24645530, Length=357, Percent_Identity=42.296918767507, Blast_Score=266, Evalue=1e-71,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006033
- InterPro:   IPR006034
- InterPro:   IPR020827 [H]

Pfam domain/function: PF00710 Asparaginase [H]

EC number: =3.5.1.1 [H]

Molecular weight: Translated: 36426; Mature: 36295

Theoretical pI: Translated: 5.60; Mature: 5.60

Prosite motif: PS00144 ASN_GLN_ASE_1 ; PS00917 ASN_GLN_ASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKRSIYVAYTGGTIGMQKTDNGFVPVAGFLTQCVQSMPEFYHEEMPTFVIHEYCPLIDS
CCCCEEEEEEECCEEECEECCCCCEEHHHHHHHHHHHHHHHHHHHCCCEEHHHHCCCCCC
SNMAPTDWQMIADDIQANYDKYDGFVILHGTDTMAYTASALSFMLQGLSKPVIVTGSQIP
CCCCCCHHHHHHHHHHCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHCCCCEEEECCCCC
LAQLRSDGQTNLLNSLYIAANYPVAEVCLFFNNKLFRGNRSTKAHADGFDAFASPNFPLL
HHHHHCCCCHHHHHEEEEEECCCHHHHHHHHCCEEEECCCCCCCCCCCCHHCCCCCCCEE
LEAGIKINLRAGKITVANDTPLEVAHISPQPVGVVTLYPGISTQIFENILQQPVKALILL
EECCEEEEEEECEEEECCCCCEEEEEECCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHE
TFGVGNAPQDAALLKTLRQADERGIVLVNLTQCFQGKVNMGGYATGNALAKAGVISGADM
EECCCCCCCHHHHHHHHHHHCCCCEEEEEEHHHHCCCCCCCCEECCCHHHHHCCCCCCCH
TIEAALAKLHYLLSKNLQPAEIKAAMLQNLVGELSID
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TKRSIYVAYTGGTIGMQKTDNGFVPVAGFLTQCVQSMPEFYHEEMPTFVIHEYCPLIDS
CCCEEEEEEECCEEECEECCCCCEEHHHHHHHHHHHHHHHHHHHCCCEEHHHHCCCCCC
SNMAPTDWQMIADDIQANYDKYDGFVILHGTDTMAYTASALSFMLQGLSKPVIVTGSQIP
CCCCCCHHHHHHHHHHCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHCCCCEEEECCCCC
LAQLRSDGQTNLLNSLYIAANYPVAEVCLFFNNKLFRGNRSTKAHADGFDAFASPNFPLL
HHHHHCCCCHHHHHEEEEEECCCHHHHHHHHCCEEEECCCCCCCCCCCCHHCCCCCCCEE
LEAGIKINLRAGKITVANDTPLEVAHISPQPVGVVTLYPGISTQIFENILQQPVKALILL
EECCEEEEEEECEEEECCCCCEEEEEECCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHE
TFGVGNAPQDAALLKTLRQADERGIVLVNLTQCFQGKVNMGGYATGNALAKAGVISGADM
EECCCCCCCHHHHHHHHHHHCCCCEEEEEEHHHHCCCCCCCCEECCCHHHHHCCCCCCCH
TIEAALAKLHYLLSKNLQPAEIKAAMLQNLVGELSID
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]