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The map label for this gene is glnA [H]

Identifier: 160875301

GI number: 160875301

Start: 2601754

End: 2603097

Strand: Direct

Name: glnA [H]

Synonym: Sbal195_2188

Alternate gene names: 160875301

Gene position: 2601754-2603097 (Clockwise)

Preceding gene: 160875300

Following gene: 160875308

Centisome position: 48.66

GC content: 48.07

Gene sequence:

>1344_bases
ATGAATCAAATGGACTCAACCCTTCTTACTGCGTTTTTCCAGCATGGCACAATACGTGATGTCGAATGTTTATTCCCTGA
TATCTCAGGCTATCCAAGGGGGAAGCTCATGCCAGCCCGAAGCTTTGCTCAGGGGGCTGAGTTACGTATTGCCCAAGCGA
TTTCGATGCAGGCGATTACCGGTGAGTATTCCTACGATCCAATTTTCCCCGATGCCGATCCTGATCTGTTACTCGTCCCC
GATTACAGCACCCTAAAAATGATCCCGTGGAGCACAGTGCCGCGCGCATTTGCCATCCATGACTGTGTGTTGCTCGATGG
CAATTTATGCCCATTTGCGCCGCGTTCTATTTTAAAAAATGTGTTGGCCCGTTACCAAGCCCTTGGGTTAACGCCCGTGG
TGGCCCCTGAGATTGAGTTTTATCTGACTGAAGCCAATACCGATCCGAGTGTGCCGTATCAGGCGCCAATTTCAAAAAGT
GGCCGAGCTGAAAACGGTCAATCTGCGTTTAGTATGAATATGCTCAATGAACTCGCGCCATTTTGGGATGAGTTTAATGC
CGCCATCGATGCGATTGGGATTAAAGGTGATACTTGGATCCATGAGATGGGGCTATCGCAATATGAAATTAACTTAGTGC
ATGGCGATCCGCTGGCCTTAGCAGATCAAGCTTTTATGTTTAAATACGCGGCGAAAGAAATCGCCATTAAACATGGCTTG
AACGCGATTTTTATGGCGAAACCCATAGCAGGCCAACCCGGCAGTTCTATGCATTTACATCAAAGTGTCGTGGATGATAA
AGGCAACAATATTTTCAGCCATGCAGATGGTAGCGATACTGAACATTTTCACCATTACATCGGCGGATTACAAAAGTATC
TACCTGATTTAATGTTGTTATTCGCACCCAATATCAATTCATTCCGACGTTACATTTCCGGTAGCCAAGCGCCGATTAAT
TTAAGCTGGGGCGTAGATAACCGCACTACAGGCTTACGAGTGCCATTGAGTGCTCCGGTGGCGCGACGGGTAGAGAATCG
CATTGCGGGCGCCGATGCCAATCCGTATTTGATGATTGCCGCATCTTTGGCTGCGGGACTTGCGGGAATGGAAGAAAAAC
TATCCCCAAGCGCGCCAGAAACCGGTAGTGCTTATGAGAGCAGCCATGACTTAGCGCAGACATTTCTCGCTGCATTAACG
CAAATGCGCCAAAGTGATTCGGCAAGGCGTTTACTCGGGGATGATTTTGTCACGGGTTTTGTGTCAGTGAAGGAGATTGA
GTTTCAAAGTTACTTGAGTGAGATAGGTGCTTGGGAGCGACGTTTCTTAGGGCCGCAATGCTAG

Upstream 100 bases:

>100_bases
TTTATATTTTACATATTTCATCGTCGCCATGTAGCTTTTCAAATCAAAGCCTGATTATACTTTGCATCCTTCATGGTGTT
ATTTTCAACATTAGTGGCAA

Downstream 100 bases:

>100_bases
TGCTTATTTTCTGAGGGGATGTTGCTGGATAACGCTTTATCCCTTGAGTGACTCGACATCCCACAAATCACGCGACTAAA
AAACAGCAACAAAAAAGCCA

Product: glutamate--putrescine ligase

Products: NA

Alternate protein names: GS; Glutamate--ammonia ligase [H]

Number of amino acids: Translated: 447; Mature: 447

Protein sequence:

>447_residues
MNQMDSTLLTAFFQHGTIRDVECLFPDISGYPRGKLMPARSFAQGAELRIAQAISMQAITGEYSYDPIFPDADPDLLLVP
DYSTLKMIPWSTVPRAFAIHDCVLLDGNLCPFAPRSILKNVLARYQALGLTPVVAPEIEFYLTEANTDPSVPYQAPISKS
GRAENGQSAFSMNMLNELAPFWDEFNAAIDAIGIKGDTWIHEMGLSQYEINLVHGDPLALADQAFMFKYAAKEIAIKHGL
NAIFMAKPIAGQPGSSMHLHQSVVDDKGNNIFSHADGSDTEHFHHYIGGLQKYLPDLMLLFAPNINSFRRYISGSQAPIN
LSWGVDNRTTGLRVPLSAPVARRVENRIAGADANPYLMIAASLAAGLAGMEEKLSPSAPETGSAYESSHDLAQTFLAALT
QMRQSDSARRLLGDDFVTGFVSVKEIEFQSYLSEIGAWERRFLGPQC

Sequences:

>Translated_447_residues
MNQMDSTLLTAFFQHGTIRDVECLFPDISGYPRGKLMPARSFAQGAELRIAQAISMQAITGEYSYDPIFPDADPDLLLVP
DYSTLKMIPWSTVPRAFAIHDCVLLDGNLCPFAPRSILKNVLARYQALGLTPVVAPEIEFYLTEANTDPSVPYQAPISKS
GRAENGQSAFSMNMLNELAPFWDEFNAAIDAIGIKGDTWIHEMGLSQYEINLVHGDPLALADQAFMFKYAAKEIAIKHGL
NAIFMAKPIAGQPGSSMHLHQSVVDDKGNNIFSHADGSDTEHFHHYIGGLQKYLPDLMLLFAPNINSFRRYISGSQAPIN
LSWGVDNRTTGLRVPLSAPVARRVENRIAGADANPYLMIAASLAAGLAGMEEKLSPSAPETGSAYESSHDLAQTFLAALT
QMRQSDSARRLLGDDFVTGFVSVKEIEFQSYLSEIGAWERRFLGPQC
>Mature_447_residues
MNQMDSTLLTAFFQHGTIRDVECLFPDISGYPRGKLMPARSFAQGAELRIAQAISMQAITGEYSYDPIFPDADPDLLLVP
DYSTLKMIPWSTVPRAFAIHDCVLLDGNLCPFAPRSILKNVLARYQALGLTPVVAPEIEFYLTEANTDPSVPYQAPISKS
GRAENGQSAFSMNMLNELAPFWDEFNAAIDAIGIKGDTWIHEMGLSQYEINLVHGDPLALADQAFMFKYAAKEIAIKHGL
NAIFMAKPIAGQPGSSMHLHQSVVDDKGNNIFSHADGSDTEHFHHYIGGLQKYLPDLMLLFAPNINSFRRYISGSQAPIN
LSWGVDNRTTGLRVPLSAPVARRVENRIAGADANPYLMIAASLAAGLAGMEEKLSPSAPETGSAYESSHDLAQTFLAALT
QMRQSDSARRLLGDDFVTGFVSVKEIEFQSYLSEIGAWERRFLGPQC

Specific function: Unknown

COG id: COG0174

COG function: function code E; Glutamine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine synthetase family [H]

Homologues:

Organism=Homo sapiens, GI221307485, Length=443, Percent_Identity=23.9277652370203, Blast_Score=109, Evalue=4e-24,
Organism=Escherichia coli, GI87081870, Length=427, Percent_Identity=31.615925058548, Blast_Score=195, Evalue=6e-51,
Organism=Escherichia coli, GI1790301, Length=331, Percent_Identity=29.3051359516616, Blast_Score=118, Evalue=9e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008147
- InterPro:   IPR014746
- InterPro:   IPR008146
- InterPro:   IPR004809 [H]

Pfam domain/function: PF00120 Gln-synt_C; PF03951 Gln-synt_N [H]

EC number: =6.3.1.2 [H]

Molecular weight: Translated: 49056; Mature: 49056

Theoretical pI: Translated: 5.01; Mature: 5.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNQMDSTLLTAFFQHGTIRDVECLFPDISGYPRGKLMPARSFAQGAELRIAQAISMQAIT
CCCHHHHHHHHHHHCCCCCCHHHCCCCCCCCCCCCCCCCHHHHCCCHHHHHHHHHHHHCC
GEYSYDPIFPDADPDLLLVPDYSTLKMIPWSTVPRAFAIHDCVLLDGNLCPFAPRSILKN
CCCCCCCCCCCCCCCEEEECCCCCEEECCCCCCCCHHHHEEEEEECCCCCCCCHHHHHHH
VLARYQALGLTPVVAPEIEFYLTEANTDPSVPYQAPISKSGRAENGQSAFSMNMLNELAP
HHHHHHHCCCCCEECCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
FWDEFNAAIDAIGIKGDTWIHEMGLSQYEINLVHGDPLALADQAFMFKYAAKEIAIKHGL
HHHHHHHHHHHCCCCCCCHHHHCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCC
NAIFMAKPIAGQPGSSMHLHQSVVDDKGNNIFSHADGSDTEHFHHYIGGLQKYLPDLMLL
CEEEEECCCCCCCCCCCHHHHHHHCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHH
FAPNINSFRRYISGSQAPINLSWGVDNRTTGLRVPLSAPVARRVENRIAGADANPYLMIA
HCCCHHHHHHHHCCCCCCEEEEECCCCCCCCEEECCCCHHHHHHHHHHCCCCCCCCEEEH
ASLAAGLAGMEEKLSPSAPETGSAYESSHDLAQTFLAALTQMRQSDSARRLLGDDFVTGF
HHHHHHHHCHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
VSVKEIEFQSYLSEIGAWERRFLGPQC
HHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MNQMDSTLLTAFFQHGTIRDVECLFPDISGYPRGKLMPARSFAQGAELRIAQAISMQAIT
CCCHHHHHHHHHHHCCCCCCHHHCCCCCCCCCCCCCCCCHHHHCCCHHHHHHHHHHHHCC
GEYSYDPIFPDADPDLLLVPDYSTLKMIPWSTVPRAFAIHDCVLLDGNLCPFAPRSILKN
CCCCCCCCCCCCCCCEEEECCCCCEEECCCCCCCCHHHHEEEEEECCCCCCCCHHHHHHH
VLARYQALGLTPVVAPEIEFYLTEANTDPSVPYQAPISKSGRAENGQSAFSMNMLNELAP
HHHHHHHCCCCCEECCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
FWDEFNAAIDAIGIKGDTWIHEMGLSQYEINLVHGDPLALADQAFMFKYAAKEIAIKHGL
HHHHHHHHHHHCCCCCCCHHHHCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCC
NAIFMAKPIAGQPGSSMHLHQSVVDDKGNNIFSHADGSDTEHFHHYIGGLQKYLPDLMLL
CEEEEECCCCCCCCCCCHHHHHHHCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHH
FAPNINSFRRYISGSQAPINLSWGVDNRTTGLRVPLSAPVARRVENRIAGADANPYLMIA
HCCCHHHHHHHHCCCCCCEEEEECCCCCCCCEEECCCCHHHHHHHHHHCCCCCCCCEEEH
ASLAAGLAGMEEKLSPSAPETGSAYESSHDLAQTFLAALTQMRQSDSARRLLGDDFVTGF
HHHHHHHHCHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
VSVKEIEFQSYLSEIGAWERRFLGPQC
HHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9679194 [H]