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The map label for this gene is hypF [H]

Identifier: 160875032

GI number: 160875032

Start: 2286027

End: 2288549

Strand: Reverse

Name: hypF [H]

Synonym: Sbal195_1917

Alternate gene names: 160875032

Gene position: 2288549-2286027 (Counterclockwise)

Preceding gene: 160875033

Following gene: 160875030

Centisome position: 42.8

GC content: 49.82

Gene sequence:

>2523_bases
ATGCCCATGATTAGGAGGCCAAAAGTTAAGTCACAACGCATGCAGCCACATAGACATAATAGTCGCCACAAAAAACACAG
TGATCTGCAGATAAAGCGCAGGCTGGCATTGGAATACCTTGTCCACAAGGGTGCCGCGCTAAAGCGTTGTCAGCTGCACA
TCACTGGCATAGTGCAAGGGGTGGGATTTCGCCCCTTTGTCTATCGTTATGCCCTTGAGTGTCAACTGACAGGTACTGTG
CTCAACAATGCCAAGGGCGTGACCATTGAGCTGCAAGGCACGGAAGAAAACCTCACGCAATTTATCACAACGCTCAATAC
CACCCCACCGCCACTGGCCCGTATCGACAATATAATCCAAACACCGCTGCCGATTTTGCTAAGCGAAACCACATTTGAAA
TTATAAAAAGCGACAGCCATGAAAACGATGGTCCAAGTTCATCAATGGGTTCGCAGGTGGCGGTATCGGCGGATAAAAGT
ACCTGCCAAGATTGCTTGAACGACATGCATAATCCTGATGATCGCCACTTTGGCTATGCTTTTACCAACTGCACAAATTG
CGGCCCAAGATACACCATCATCAATGCCTTACCCTATGATCGCCACAGTACCGCCATGGCCGATTTTGACATGTGCCCCG
AGTGCGCCAAAGCATATCAAAACCCACTCGACCGCCGCTATCATGCTCAGCCAGTCAGTTGCCCCGCTTGCGGCCCCAAA
CTCAGTTTAAAAACACCCCAAGGGGAATACATCATTCCCAATACCTCTAATTTCAGCACACCTGAATTGAATACGGATTT
GGCGGTACTGAAAGCGGCGGCTGACAGATTACACCAAGGGCAGATTCTGGCAATTAAGGGACTGGGCGGATTTCACTTAA
TCTGTGATGCCCGTAACGACAAAGCAGTCAACAAACTCCGGCTGCGAAAACAGCGCCAAGCCAAGCCGCTAGCTGTGATG
ATGGCGGATATCCAGATGGCGCAGCACTATGTCACAGGGACGGATGCTGAGTGGCAAGTACTGAGTTCGCAGGAGCGGCC
CATAGTGCTGATGAGCAGCCGCGCCGAACATGATCTCAGCTTAGCTGTCGCCCCTAATCTGAATAAACTCGGGGTATTTT
TGCCCTACACACCACTGCATCATTTACTGTTAGGTCTCTTCAAAGGGCCATTGGTCGCGACCAGCGCTAATGTGTCCGGA
GAACCTATCATCACAAATTGTGATGAACTTATTCGCCACTTAAGCCATGTGGTGGATGCAATAGTTGATCATAATCGGCC
GATTATTAATGGCTGCGATGACAGTGTGGTGCAGGTGATCCAGCCCCACATGGATTCGCCTGCAGAGGTGCAAGTACTGC
GTCTCGCCCGTGGTTATGCGCCCTTAAGTTTCCCACTTGATACACCGCTAACCCAATCAATTTTGGCCGTGGGCGCACAG
CAAAAAAATGCTATCGCCCTAGGCTTTGGCAACAATGTGTTTTTAAGTCCCCATATTGGCGATTTATTCAGTGTCAGCGC
CGAGCAGTATTTTGAGCGCACCTTAGCCACCTTCGCGCGGCTGTATCACTTTGCGCCCAATCTCATTGTTCGCGATAAAC
ACCCAGATTATGCCCCATCGCGCTGGGCTGAAATGCACTCAAACTCGAGTGTAGAACTCATCAATACCAGCCAAAGCCAA
TGTTTAACGGTACAACATCACCATGCCCATGTGCTCAGCGTAATGGCGGTTAATCAATATCGAGATCCCGTATTGGGCTT
TAGTTTTGATGGCACAGGGCTTGGCGACGATGGCAGTTTATGGGGCGGTGAAGTGCTATTAACCACACTGGATAAAGCGC
AGCGACTGGCCCATTTTGAGCCTATAGCCCTGCTGGGCGGCGAGCAAGCCATTAAGCAACCTGTGCGGTTGTTACTGGCA
TTATTGTTCGAGCAAATGAGCCTCGATGCTGTACTCGCATTGCAATTACCTATATTAAACAACATGCGCCCAAACACTCT
GACTAACTTACATAAGATCTGGCAGAACGGTAGCGTACTTAAAAGCTCATCGGTCGGACGCTTGTTCGATGCCCTCGCCT
GCGCCTTAGGCTTAATCGAAACCACACAATTTGAAGGTCAAGCGGGCATGGCAATAGAAGCCGCTGCCAATCGCGCCGAT
GCATTAGCCCTTGAAGCCATGTCGCTTAACTTACCTCTTGACGTCGACCAGTGGCGCAGCAGTGAAATGTTCAAGCAGAT
TGTTGAATTAATAACTGAAAAACCGCTAACAGACAACAGACGAGATGCCATCGCCCGCGCCTTTTTACACACATTAGGCG
ATGCTATCTGCGATTATGCTGCCCAGTACAAACACCTTCCAACCGTGCTCTGCGGCGGCGTATTCCAAAATAAATACCTA
TTGGAATATTGCCTGAGCAAACTCTGCGCCCAAGGCAATAAAGTGTTATCCAGTCAACAGATCCCCATCAATGATGGCGG
CATCGCCCTAGGCCAACTCTGGTATGGGATGCATAGAAACTAG

Upstream 100 bases:

>100_bases
CGCTTAGGAAAGAACTTCCCCTTAGTGATCAATCGTAGTCTAGATTTAGACGGCGATAACTTAGCCGTTGGCGCACTTTA
CGTTAAGCAGCGCCAACTGA

Downstream 100 bases:

>100_bases
TCACGATAAAACCTAAGCGTGTAGCAGTGATGAAGAAAATAAAAACGAGCCTCATTGAGGCTCGTTTTATTCTTCAACAC
AATTGCGGGCACAGAGTGCT

Product: (NiFe) hydrogenase maturation protein HypF

Products: NA

Alternate protein names: Carbamoyl phosphate-converting enzyme hypF; [NiFe]-hydrogenase maturation factor hypF; Hydrogenase maturation protein hypF [H]

Number of amino acids: Translated: 840; Mature: 839

Protein sequence:

>840_residues
MPMIRRPKVKSQRMQPHRHNSRHKKHSDLQIKRRLALEYLVHKGAALKRCQLHITGIVQGVGFRPFVYRYALECQLTGTV
LNNAKGVTIELQGTEENLTQFITTLNTTPPPLARIDNIIQTPLPILLSETTFEIIKSDSHENDGPSSSMGSQVAVSADKS
TCQDCLNDMHNPDDRHFGYAFTNCTNCGPRYTIINALPYDRHSTAMADFDMCPECAKAYQNPLDRRYHAQPVSCPACGPK
LSLKTPQGEYIIPNTSNFSTPELNTDLAVLKAAADRLHQGQILAIKGLGGFHLICDARNDKAVNKLRLRKQRQAKPLAVM
MADIQMAQHYVTGTDAEWQVLSSQERPIVLMSSRAEHDLSLAVAPNLNKLGVFLPYTPLHHLLLGLFKGPLVATSANVSG
EPIITNCDELIRHLSHVVDAIVDHNRPIINGCDDSVVQVIQPHMDSPAEVQVLRLARGYAPLSFPLDTPLTQSILAVGAQ
QKNAIALGFGNNVFLSPHIGDLFSVSAEQYFERTLATFARLYHFAPNLIVRDKHPDYAPSRWAEMHSNSSVELINTSQSQ
CLTVQHHHAHVLSVMAVNQYRDPVLGFSFDGTGLGDDGSLWGGEVLLTTLDKAQRLAHFEPIALLGGEQAIKQPVRLLLA
LLFEQMSLDAVLALQLPILNNMRPNTLTNLHKIWQNGSVLKSSSVGRLFDALACALGLIETTQFEGQAGMAIEAAANRAD
ALALEAMSLNLPLDVDQWRSSEMFKQIVELITEKPLTDNRRDAIARAFLHTLGDAICDYAAQYKHLPTVLCGGVFQNKYL
LEYCLSKLCAQGNKVLSSQQIPINDGGIALGQLWYGMHRN

Sequences:

>Translated_840_residues
MPMIRRPKVKSQRMQPHRHNSRHKKHSDLQIKRRLALEYLVHKGAALKRCQLHITGIVQGVGFRPFVYRYALECQLTGTV
LNNAKGVTIELQGTEENLTQFITTLNTTPPPLARIDNIIQTPLPILLSETTFEIIKSDSHENDGPSSSMGSQVAVSADKS
TCQDCLNDMHNPDDRHFGYAFTNCTNCGPRYTIINALPYDRHSTAMADFDMCPECAKAYQNPLDRRYHAQPVSCPACGPK
LSLKTPQGEYIIPNTSNFSTPELNTDLAVLKAAADRLHQGQILAIKGLGGFHLICDARNDKAVNKLRLRKQRQAKPLAVM
MADIQMAQHYVTGTDAEWQVLSSQERPIVLMSSRAEHDLSLAVAPNLNKLGVFLPYTPLHHLLLGLFKGPLVATSANVSG
EPIITNCDELIRHLSHVVDAIVDHNRPIINGCDDSVVQVIQPHMDSPAEVQVLRLARGYAPLSFPLDTPLTQSILAVGAQ
QKNAIALGFGNNVFLSPHIGDLFSVSAEQYFERTLATFARLYHFAPNLIVRDKHPDYAPSRWAEMHSNSSVELINTSQSQ
CLTVQHHHAHVLSVMAVNQYRDPVLGFSFDGTGLGDDGSLWGGEVLLTTLDKAQRLAHFEPIALLGGEQAIKQPVRLLLA
LLFEQMSLDAVLALQLPILNNMRPNTLTNLHKIWQNGSVLKSSSVGRLFDALACALGLIETTQFEGQAGMAIEAAANRAD
ALALEAMSLNLPLDVDQWRSSEMFKQIVELITEKPLTDNRRDAIARAFLHTLGDAICDYAAQYKHLPTVLCGGVFQNKYL
LEYCLSKLCAQGNKVLSSQQIPINDGGIALGQLWYGMHRN
>Mature_839_residues
PMIRRPKVKSQRMQPHRHNSRHKKHSDLQIKRRLALEYLVHKGAALKRCQLHITGIVQGVGFRPFVYRYALECQLTGTVL
NNAKGVTIELQGTEENLTQFITTLNTTPPPLARIDNIIQTPLPILLSETTFEIIKSDSHENDGPSSSMGSQVAVSADKST
CQDCLNDMHNPDDRHFGYAFTNCTNCGPRYTIINALPYDRHSTAMADFDMCPECAKAYQNPLDRRYHAQPVSCPACGPKL
SLKTPQGEYIIPNTSNFSTPELNTDLAVLKAAADRLHQGQILAIKGLGGFHLICDARNDKAVNKLRLRKQRQAKPLAVMM
ADIQMAQHYVTGTDAEWQVLSSQERPIVLMSSRAEHDLSLAVAPNLNKLGVFLPYTPLHHLLLGLFKGPLVATSANVSGE
PIITNCDELIRHLSHVVDAIVDHNRPIINGCDDSVVQVIQPHMDSPAEVQVLRLARGYAPLSFPLDTPLTQSILAVGAQQ
KNAIALGFGNNVFLSPHIGDLFSVSAEQYFERTLATFARLYHFAPNLIVRDKHPDYAPSRWAEMHSNSSVELINTSQSQC
LTVQHHHAHVLSVMAVNQYRDPVLGFSFDGTGLGDDGSLWGGEVLLTTLDKAQRLAHFEPIALLGGEQAIKQPVRLLLAL
LFEQMSLDAVLALQLPILNNMRPNTLTNLHKIWQNGSVLKSSSVGRLFDALACALGLIETTQFEGQAGMAIEAAANRADA
LALEAMSLNLPLDVDQWRSSEMFKQIVELITEKPLTDNRRDAIARAFLHTLGDAICDYAAQYKHLPTVLCGGVFQNKYLL
EYCLSKLCAQGNKVLSSQQIPINDGGIALGQLWYGMHRN

Specific function: Along with hypE, it catalyzes the synthesis of the CN ligands of the active site iron of [NiFe]-hydrogenases using carbamoylphosphate as a substrate. It functions as a carbamoyl transferase using carbamoylphosphate as a substrate and transferring the carb

COG id: COG0068

COG function: function code O; Hydrogenase maturation factor

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 YrdC-like domain [H]

Homologues:

Organism=Escherichia coli, GI2367152, Length=797, Percent_Identity=36.2609786700125, Blast_Score=381, Evalue=1e-107,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001792
- InterPro:   IPR017968
- InterPro:   IPR004421
- InterPro:   IPR017945
- InterPro:   IPR006070
- InterPro:   IPR011125 [H]

Pfam domain/function: PF00708 Acylphosphatase; PF01300 Sua5_yciO_yrdC; PF07503 zf-HYPF [H]

EC number: NA

Molecular weight: Translated: 92839; Mature: 92708

Theoretical pI: Translated: 7.34; Mature: 7.34

Prosite motif: PS00150 ACYLPHOSPHATASE_1 ; PS51160 ACYLPHOSPHATASE_3 ; PS51163 YRDC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPMIRRPKVKSQRMQPHRHNSRHKKHSDLQIKRRLALEYLVHKGAALKRCQLHITGIVQG
CCCCCCCCCHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHEEEEEEHHHC
VGFRPFVYRYALECQLTGTVLNNAKGVTIELQGTEENLTQFITTLNTTPPPLARIDNIIQ
CCCCHHHHHEEEEEEEEEHHHCCCCCEEEEEECCHHHHHHHHHHHCCCCCCHHHHHHHHH
TPLPILLSETTFEIIKSDSHENDGPSSSMGSQVAVSADKSTCQDCLNDMHNPDDRHFGYA
CCHHHHCCHHHHHHHHCCCCCCCCCCCCCCCEEEEECCHHHHHHHHHHCCCCCCCCCCEE
FTNCTNCGPRYTIINALPYDRHSTAMADFDMCPECAKAYQNPLDRRYHAQPVSCPACGPK
ECCCCCCCCCEEEEECCCCCCCCCHHHHHHCCHHHHHHHHCHHHHCCCCCCCCCCCCCCC
LSLKTPQGEYIIPNTSNFSTPELNTDLAVLKAAADRLHQGQILAIKGLGGFHLICDARND
CEEECCCCCEECCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCEEEEEECCCC
KAVNKLRLRKQRQAKPLAVMMADIQMAQHYVTGTDAEWQVLSSQERPIVLMSSRAEHDLS
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCHHEECCCCCCEEEEECCCCCCEE
LAVAPNLNKLGVFLPYTPLHHLLLGLFKGPLVATSANVSGEPIITNCDELIRHLSHVVDA
EEECCCCCCCEEEECCHHHHHHHHHHHCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHH
IVDHNRPIINGCDDSVVQVIQPHMDSPAEVQVLRLARGYAPLSFPLDTPLTQSILAVGAQ
HHHCCCCEECCCCHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHCCC
QKNAIALGFGNNVFLSPHIGDLFSVSAEQYFERTLATFARLYHFAPNLIVRDKHPDYAPS
CCCEEEEECCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCH
RWAEMHSNSSVELINTSQSQCLTVQHHHAHVLSVMAVNQYRDPVLGFSFDGTGLGDDGSL
HHHHHCCCCCEEEEECCCCCEEEEEHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCC
WGGEVLLTTLDKAQRLAHFEPIALLGGEQAIKQPVRLLLALLFEQMSLDAVLALQLPILN
CCCHHHHHHHHHHHHHHCCCCCEEECCHHHHHHHHHHHHHHHHHHHCCCEEEEEECHHHC
NMRPNTLTNLHKIWQNGSVLKSSSVGRLFDALACALGLIETTQFEGQAGMAIEAAANRAD
CCCCHHHHHHHHHHCCCCEEECCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEEHHCCCHH
ALALEAMSLNLPLDVDQWRSSEMFKQIVELITEKPLTDNRRDAIARAFLHTLGDAICDYA
HHEEEEEECCCCCCHHHHCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHH
AQYKHLPTVLCGGVFQNKYLLEYCLSKLCAQGNKVLSSQQIPINDGGIALGQLWYGMHRN
HHHHCCCHHHHHHHHCCHHHHHHHHHHHHHCCCHHHCCCCCCCCCCCCHHHHHHHHHCCC
>Mature Secondary Structure 
PMIRRPKVKSQRMQPHRHNSRHKKHSDLQIKRRLALEYLVHKGAALKRCQLHITGIVQG
CCCCCCCCHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHEEEEEEHHHC
VGFRPFVYRYALECQLTGTVLNNAKGVTIELQGTEENLTQFITTLNTTPPPLARIDNIIQ
CCCCHHHHHEEEEEEEEEHHHCCCCCEEEEEECCHHHHHHHHHHHCCCCCCHHHHHHHHH
TPLPILLSETTFEIIKSDSHENDGPSSSMGSQVAVSADKSTCQDCLNDMHNPDDRHFGYA
CCHHHHCCHHHHHHHHCCCCCCCCCCCCCCCEEEEECCHHHHHHHHHHCCCCCCCCCCEE
FTNCTNCGPRYTIINALPYDRHSTAMADFDMCPECAKAYQNPLDRRYHAQPVSCPACGPK
ECCCCCCCCCEEEEECCCCCCCCCHHHHHHCCHHHHHHHHCHHHHCCCCCCCCCCCCCCC
LSLKTPQGEYIIPNTSNFSTPELNTDLAVLKAAADRLHQGQILAIKGLGGFHLICDARND
CEEECCCCCEECCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCEEEEEECCCC
KAVNKLRLRKQRQAKPLAVMMADIQMAQHYVTGTDAEWQVLSSQERPIVLMSSRAEHDLS
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCHHEECCCCCCEEEEECCCCCCEE
LAVAPNLNKLGVFLPYTPLHHLLLGLFKGPLVATSANVSGEPIITNCDELIRHLSHVVDA
EEECCCCCCCEEEECCHHHHHHHHHHHCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHH
IVDHNRPIINGCDDSVVQVIQPHMDSPAEVQVLRLARGYAPLSFPLDTPLTQSILAVGAQ
HHHCCCCEECCCCHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHCCC
QKNAIALGFGNNVFLSPHIGDLFSVSAEQYFERTLATFARLYHFAPNLIVRDKHPDYAPS
CCCEEEEECCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCH
RWAEMHSNSSVELINTSQSQCLTVQHHHAHVLSVMAVNQYRDPVLGFSFDGTGLGDDGSL
HHHHHCCCCCEEEEECCCCCEEEEEHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCC
WGGEVLLTTLDKAQRLAHFEPIALLGGEQAIKQPVRLLLALLFEQMSLDAVLALQLPILN
CCCHHHHHHHHHHHHHHCCCCCEEECCHHHHHHHHHHHHHHHHHHHCCCEEEEEECHHHC
NMRPNTLTNLHKIWQNGSVLKSSSVGRLFDALACALGLIETTQFEGQAGMAIEAAANRAD
CCCCHHHHHHHHHHCCCCEEECCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEEHHCCCHH
ALALEAMSLNLPLDVDQWRSSEMFKQIVELITEKPLTDNRRDAIARAFLHTLGDAICDYA
HHEEEEEECCCCCCHHHHCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHH
AQYKHLPTVLCGGVFQNKYLLEYCLSKLCAQGNKVLSSQQIPINDGGIALGQLWYGMHRN
HHHHCCCHHHHHHHHCCHHHHHHHHHHHHHCCCHHHCCCCCCCCCCCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]