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The map label for this gene is puuB [H]

Identifier: 160874322

GI number: 160874322

Start: 1426846

End: 1428132

Strand: Direct

Name: puuB [H]

Synonym: Sbal195_1203

Alternate gene names: 160874322

Gene position: 1426846-1428132 (Clockwise)

Preceding gene: 160874321

Following gene: 160874323

Centisome position: 26.68

GC content: 50.74

Gene sequence:

>1287_bases
ATGTCTGCCATAGCTCACGCTGGATCATATTATGCGGCATCGGCCAACGATAAGGTTGAACGTCCGCGCCTAGAGGAATC
GATTGAAGCCGATGTTTGTGTGATTGGCGCGGGTTATACGGGCCTTTCTGCCGCGCTGCATTTATTAGAATCTGGCTTTA
GTGTGGTGGTGTTAGAAGCCGCTCGCATTGGCTGGGGCGCCTCGGGCCGTAATGGCGGACAAATCGTCAATAGTTTTAGC
CGTGACATTGATACTATTGAAAAAACAGTCGGCAAAGAACAAGGTAAACTCTTTGGCCAAATGGCTTTTGAGGGCGGAAA
AATCATCCGCGATCGGATTGCTAAGTACAACATTCAATGCGATCTAAAAGATGGCGGCGTGTTTGCCGCGATGAACGAAA
AACAAATGGGTCATCTGCGCCATCAAAAGCAACTGTGGGAAAGCCACGGTCATGTTAATCAGCTCGAACTTCTCGATGCC
AAAGGCATTCGCGGTGTGGTAAATACTGAGCGCTATGTCGGTGGTCTGCTCGATAAAAGCGGCGGCCATATTCATCCGCT
TAACTTAGCTCTTGGCGAAGCGCGTGCGGTCGAATCCCTCGGCGGCAAGATTTTTGAAGACTCAGCAGTGCTGAGTGTCG
ATGAAGGCGATAGCCCAGTTGTGCATACGGCGCAGGGCAGTGTCAAAGCCAAGTTTGTGGTCGTGGCGGGTAATGCTTAT
TTAGGTAAGTTAATGCCAGAGCTGCAGGCGAAATCTATGCCCTGCGGCACGCAAGTGATTACCACTGAGCCTTTGAGTGA
CGATTTGGCGGCGAGCTTATTACCGCAGGATTACTGCGTCGAAGACTGTAACTATCTACTCGATTATTTCCGCCTGTCTG
GCGACAAGCGGATGATTTACGGCGGCGGCGTAGTGTATGGCGCCCGCGATCCGGCGGATATCAAGTCGATCATCATTCCG
AATATGCTCAAAACCTTTCCGCAATTAAAAGATGTGAAGATCGATTATGCGTGGACAGGTAACTTCCTACTGACGCTATC
GCGCCTGCCACAGGTCGGTCGTATCGGTAAGAATATCTATTATTCACAGGGTTGCAGTGGCCACGGTGTCACTTATACCC
ATCTGGCAGGCAAGTTACTGGCCGAAATGCTCAACGGCCAAGCCACGCGTTTCGACGCCTTTGCTGCATTGCCACATTAT
CCATTCCCCGGTGGTCACGCACTTCGCGTCCCTTTTAGTGCTATCGGAGCTTGGTATTACACGTTAAGAGACAAACTGGG
TCTCTAA

Upstream 100 bases:

>100_bases
ATGTAAATTATTGCACTTTTCTGGCTAGTGTTTCGTTTTCTGAAGGTGTAGGATGTGATGGCTGTTTAAAATAATGAACA
TTAGAATTCAAGGGAACAAC

Downstream 100 bases:

>100_bases
TATCATCACTTTTAAGAAGCTAGGTAGCTTCACAATACGAGGAAATCCTTGTGCAATTAAATGATCCGAGTTTGCTACAT
CAACAGTCTTTTATTAATGG

Product: FAD dependent oxidoreductase

Products: NA

Alternate protein names: Gamma-Glu-Put oxidase; Gamma-glutamylputrescine oxidase [H]

Number of amino acids: Translated: 428; Mature: 427

Protein sequence:

>428_residues
MSAIAHAGSYYAASANDKVERPRLEESIEADVCVIGAGYTGLSAALHLLESGFSVVVLEAARIGWGASGRNGGQIVNSFS
RDIDTIEKTVGKEQGKLFGQMAFEGGKIIRDRIAKYNIQCDLKDGGVFAAMNEKQMGHLRHQKQLWESHGHVNQLELLDA
KGIRGVVNTERYVGGLLDKSGGHIHPLNLALGEARAVESLGGKIFEDSAVLSVDEGDSPVVHTAQGSVKAKFVVVAGNAY
LGKLMPELQAKSMPCGTQVITTEPLSDDLAASLLPQDYCVEDCNYLLDYFRLSGDKRMIYGGGVVYGARDPADIKSIIIP
NMLKTFPQLKDVKIDYAWTGNFLLTLSRLPQVGRIGKNIYYSQGCSGHGVTYTHLAGKLLAEMLNGQATRFDAFAALPHY
PFPGGHALRVPFSAIGAWYYTLRDKLGL

Sequences:

>Translated_428_residues
MSAIAHAGSYYAASANDKVERPRLEESIEADVCVIGAGYTGLSAALHLLESGFSVVVLEAARIGWGASGRNGGQIVNSFS
RDIDTIEKTVGKEQGKLFGQMAFEGGKIIRDRIAKYNIQCDLKDGGVFAAMNEKQMGHLRHQKQLWESHGHVNQLELLDA
KGIRGVVNTERYVGGLLDKSGGHIHPLNLALGEARAVESLGGKIFEDSAVLSVDEGDSPVVHTAQGSVKAKFVVVAGNAY
LGKLMPELQAKSMPCGTQVITTEPLSDDLAASLLPQDYCVEDCNYLLDYFRLSGDKRMIYGGGVVYGARDPADIKSIIIP
NMLKTFPQLKDVKIDYAWTGNFLLTLSRLPQVGRIGKNIYYSQGCSGHGVTYTHLAGKLLAEMLNGQATRFDAFAALPHY
PFPGGHALRVPFSAIGAWYYTLRDKLGL
>Mature_427_residues
SAIAHAGSYYAASANDKVERPRLEESIEADVCVIGAGYTGLSAALHLLESGFSVVVLEAARIGWGASGRNGGQIVNSFSR
DIDTIEKTVGKEQGKLFGQMAFEGGKIIRDRIAKYNIQCDLKDGGVFAAMNEKQMGHLRHQKQLWESHGHVNQLELLDAK
GIRGVVNTERYVGGLLDKSGGHIHPLNLALGEARAVESLGGKIFEDSAVLSVDEGDSPVVHTAQGSVKAKFVVVAGNAYL
GKLMPELQAKSMPCGTQVITTEPLSDDLAASLLPQDYCVEDCNYLLDYFRLSGDKRMIYGGGVVYGARDPADIKSIIIPN
MLKTFPQLKDVKIDYAWTGNFLLTLSRLPQVGRIGKNIYYSQGCSGHGVTYTHLAGKLLAEMLNGQATRFDAFAALPHYP
FPGGHALRVPFSAIGAWYYTLRDKLGL

Specific function: Involved in the breakdown of putrescine via the oxidation of L-glutamylputrescine [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1787559, Length=424, Percent_Identity=65.8018867924528, Blast_Score=590, Evalue=1e-170,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006076 [H]

Pfam domain/function: PF01266 DAO [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 46294; Mature: 46163

Theoretical pI: Translated: 7.17; Mature: 7.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAIAHAGSYYAASANDKVERPRLEESIEADVCVIGAGYTGLSAALHLLESGFSVVVLEA
CCCCCCCCCEEEECCCCCCCCCCHHHHCCCCEEEEECCCHHHHHHHHHHHCCCEEEEEEE
ARIGWGASGRNGGQIVNSFSRDIDTIEKTVGKEQGKLFGQMAFEGGKIIRDRIAKYNIQC
HHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCHHHHHHHHHEEEEE
DLKDGGVFAAMNEKQMGHLRHQKQLWESHGHVNQLELLDAKGIRGVVNTERYVGGLLDKS
EECCCCEEEEECCHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCHHHHHHHHCCC
GGHIHPLNLALGEARAVESLGGKIFEDSAVLSVDEGDSPVVHTAQGSVKAKFVVVAGNAY
CCEEEEEHEEHHHHHHHHHHCCEEECCCEEEEECCCCCCEEEECCCCCEEEEEEEECCHH
LGKLMPELQAKSMPCGTQVITTEPLSDDLAASLLPQDYCVEDCNYLLDYFRLSGDKRMIY
HHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCEEEE
GGGVVYGARDPADIKSIIIPNMLKTFPQLKDVKIDYAWTGNFLLTLSRLPQVGRIGKNIY
ECEEEECCCCCHHHHHHHHHHHHHHCCCHHCEEEEEEECCCCEEEEHHCCCHHHHCCCEE
YSQGCSGHGVTYTHLAGKLLAEMLNGQATRFDAFAALPHYPFPGGHALRVPFSAIGAWYY
ECCCCCCCCEEHHHHHHHHHHHHHCCCCCEEHHHHCCCCCCCCCCCEEECCHHHHHHHHH
TLRDKLGL
HHHHHHCC
>Mature Secondary Structure 
SAIAHAGSYYAASANDKVERPRLEESIEADVCVIGAGYTGLSAALHLLESGFSVVVLEA
CCCCCCCCEEEECCCCCCCCCCHHHHCCCCEEEEECCCHHHHHHHHHHHCCCEEEEEEE
ARIGWGASGRNGGQIVNSFSRDIDTIEKTVGKEQGKLFGQMAFEGGKIIRDRIAKYNIQC
HHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCHHHHHHHHHEEEEE
DLKDGGVFAAMNEKQMGHLRHQKQLWESHGHVNQLELLDAKGIRGVVNTERYVGGLLDKS
EECCCCEEEEECCHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCHHHHHHHHCCC
GGHIHPLNLALGEARAVESLGGKIFEDSAVLSVDEGDSPVVHTAQGSVKAKFVVVAGNAY
CCEEEEEHEEHHHHHHHHHHCCEEECCCEEEEECCCCCCEEEECCCCCEEEEEEEECCHH
LGKLMPELQAKSMPCGTQVITTEPLSDDLAASLLPQDYCVEDCNYLLDYFRLSGDKRMIY
HHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCEEEE
GGGVVYGARDPADIKSIIIPNMLKTFPQLKDVKIDYAWTGNFLLTLSRLPQVGRIGKNIY
ECEEEECCCCCHHHHHHHHHHHHHHCCCHHCEEEEEEECCCCEEEEHHCCCHHHHCCCEE
YSQGCSGHGVTYTHLAGKLLAEMLNGQATRFDAFAALPHYPFPGGHALRVPFSAIGAWYY
ECCCCCCCCEEHHHHHHHHHHHHHCCCCCEEHHHHCCCCCCCCCCCEEECCHHHHHHHHH
TLRDKLGL
HHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503; 1840553; 7984428 [H]