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The map label for this gene is rsuA [H]

Identifier: 160874235

GI number: 160874235

Start: 1313768

End: 1314538

Strand: Direct

Name: rsuA [H]

Synonym: Sbal195_1115

Alternate gene names: 160874235

Gene position: 1313768-1314538 (Clockwise)

Preceding gene: 160874233

Following gene: 160874239

Centisome position: 24.57

GC content: 46.69

Gene sequence:

>771_bases
ATGAGCCGAACTTGCATCCTGCTAGAATGGCTATCTCATTCATATTTCGTCATTTGTGGTGTTATGCAATCTAAGCGCGG
CCGACTCGATCGTTATCTTTGTACCCAGTTACACACTCACCGTAAAGCGGTGCGTGAACTGCTATTATCGGGCCGAGTGT
GCGTCGATGGATTGATAGTGAAAGATATGGATAGGCAAGTGGATGAGTTTTCCCACATCCAACTCGATGAGCAAATACTG
CAGGCTAATGTGCCCGTCTATGTGATGCTCCACAAGCCGATTGGAGTGGTGAGTGCTACCAAAGATACTCAGCATAAAAC
TGTGATTGATTTGCTTGATTGCGAAGCCCGTGATGAGTTGCATATCGCCGGCAGATTAGATTTAAACTCTTCGGGTTTGT
TGTTATTGACCAATGACAGCCGCTGGTCTGATGCGCTGATGTCACCTACACACAAGGTCGATAAAGTGTATCGAGTTACC
TTGGCTAATCCCATAACCGAAGAATACATCGCTGCATTTGCCGCAGGGATGTACTTTGGCTTTGAAGATATTACGACTCA
ACCGGCTAAATTGGTTATCCTTGCTGATAACGTGGCTGAAGTGACGTTACATGAAGGTAAGTATCATCAAATCAAGCGGA
TGTTTGGTCGATTCCGCAATCCGGTTGTTGGCCTGCATCGATTGTCGATAGGCGATATTGTGCTCGATGAAAAACTCGCT
CCGGGTGAAAGTCGCGCACTCACTGCTGCTGAAGTGGCCTCTATCCGTTAA

Upstream 100 bases:

>100_bases
ACAGTAAGATGAATTGAGCGCCTAGCTTAACAAATTTTACCTCGTTCGATCCAATTTAGCTCTGAATTGCCTTTGTGAAG
ATTCGCAGCATTGGCTCGCT

Downstream 100 bases:

>100_bases
AAATGATGAACAGAAAAGTACATCAAGCATTCATCGTTCTTGTCGTTATTGTTTGCCATGCTAACAATGAAAGCTAAGCA
TAAAAGCTAAGCATAAAAGC

Product: pseudouridine synthase

Products: pseudouridine 5'-phosphate; H2O [C]

Alternate protein names: 16S pseudouridine 516 synthase; 16S pseudouridylate 516 synthase; rRNA pseudouridylate synthase A; rRNA-uridine isomerase A [H]

Number of amino acids: Translated: 256; Mature: 255

Protein sequence:

>256_residues
MSRTCILLEWLSHSYFVICGVMQSKRGRLDRYLCTQLHTHRKAVRELLLSGRVCVDGLIVKDMDRQVDEFSHIQLDEQIL
QANVPVYVMLHKPIGVVSATKDTQHKTVIDLLDCEARDELHIAGRLDLNSSGLLLLTNDSRWSDALMSPTHKVDKVYRVT
LANPITEEYIAAFAAGMYFGFEDITTQPAKLVILADNVAEVTLHEGKYHQIKRMFGRFRNPVVGLHRLSIGDIVLDEKLA
PGESRALTAAEVASIR

Sequences:

>Translated_256_residues
MSRTCILLEWLSHSYFVICGVMQSKRGRLDRYLCTQLHTHRKAVRELLLSGRVCVDGLIVKDMDRQVDEFSHIQLDEQIL
QANVPVYVMLHKPIGVVSATKDTQHKTVIDLLDCEARDELHIAGRLDLNSSGLLLLTNDSRWSDALMSPTHKVDKVYRVT
LANPITEEYIAAFAAGMYFGFEDITTQPAKLVILADNVAEVTLHEGKYHQIKRMFGRFRNPVVGLHRLSIGDIVLDEKLA
PGESRALTAAEVASIR
>Mature_255_residues
SRTCILLEWLSHSYFVICGVMQSKRGRLDRYLCTQLHTHRKAVRELLLSGRVCVDGLIVKDMDRQVDEFSHIQLDEQILQ
ANVPVYVMLHKPIGVVSATKDTQHKTVIDLLDCEARDELHIAGRLDLNSSGLLLLTNDSRWSDALMSPTHKVDKVYRVTL
ANPITEEYIAAFAAGMYFGFEDITTQPAKLVILADNVAEVTLHEGKYHQIKRMFGRFRNPVVGLHRLSIGDIVLDEKLAP
GESRALTAAEVASIR

Specific function: Responsible for synthesis of pseudouridine from uracil- 516 in 16S ribosomal RNA [H]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788510, Length=229, Percent_Identity=41.0480349344978, Blast_Score=171, Evalue=6e-44,
Organism=Escherichia coli, GI1790453, Length=228, Percent_Identity=29.8245614035088, Blast_Score=78, Evalue=6e-16,
Organism=Escherichia coli, GI1787524, Length=244, Percent_Identity=28.2786885245902, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI87081838, Length=202, Percent_Identity=29.2079207920792, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: 4.2.1.70 [C]

Molecular weight: Translated: 28835; Mature: 28704

Theoretical pI: Translated: 7.10; Mature: 7.10

Prosite motif: PS50889 S4 ; PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRTCILLEWLSHSYFVICGVMQSKRGRLDRYLCTQLHTHRKAVRELLLSGRVCVDGLIV
CCCEEEEHHHHCCCEEEEEEHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
KDMDRQVDEFSHIQLDEQILQANVPVYVMLHKPIGVVSATKDTQHKTVIDLLDCEARDEL
HHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCEEECCCCCCHHHHHHHHCCCCCCCE
HIAGRLDLNSSGLLLLTNDSRWSDALMSPTHKVDKVYRVTLANPITEEYIAAFAAGMYFG
EEEEEEEECCCCEEEEECCCCCHHHHCCCHHHHHHEEEEEECCCHHHHHHHHHHHHHHCC
FEDITTQPAKLVILADNVAEVTLHEGKYHQIKRMFGRFRNPVVGLHRLSIGDIVLDEKLA
HHHCCCCCEEEEEEECCCEEEEEECCCHHHHHHHHHHHCCCEEEEEEEEECCEEECCCCC
PGESRALTAAEVASIR
CCCCCCCHHHHHHCCC
>Mature Secondary Structure 
SRTCILLEWLSHSYFVICGVMQSKRGRLDRYLCTQLHTHRKAVRELLLSGRVCVDGLIV
CCEEEEHHHHCCCEEEEEEHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
KDMDRQVDEFSHIQLDEQILQANVPVYVMLHKPIGVVSATKDTQHKTVIDLLDCEARDEL
HHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCEEECCCCCCHHHHHHHHCCCCCCCE
HIAGRLDLNSSGLLLLTNDSRWSDALMSPTHKVDKVYRVTLANPITEEYIAAFAAGMYFG
EEEEEEEECCCCEEEEECCCCCHHHHCCCHHHHHHEEEEEECCCHHHHHHHHHHHHHHCC
FEDITTQPAKLVILADNVAEVTLHEGKYHQIKRMFGRFRNPVVGLHRLSIGDIVLDEKLA
HHHCCCCCEEEEEEECCCEEEEEECCCHHHHHHHHHHHCCCEEEEEEEEECCEEECCCCC
PGESRALTAAEVASIR
CCCCCCCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose phosphate [C]

Specific reaction: uracil + D-ribose phosphate = pseudouridine 5'-phosphate + H2O [C]

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]