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The map label for this gene is macA [H]

Identifier: 160873757

GI number: 160873757

Start: 759676

End: 760896

Strand: Direct

Name: macA [H]

Synonym: Sbal195_0635

Alternate gene names: 160873757

Gene position: 759676-760896 (Clockwise)

Preceding gene: 160873756

Following gene: 160873758

Centisome position: 14.21

GC content: 43.98

Gene sequence:

>1221_bases
ATGAAAAAGAACATTAAAAACACCTTGCTGCTTCTTATCCTTGGCGCAACGGTTGCTGTTGTAGGATACAAGTACACTCA
AGTACAACAGGATCAACCTATCTTTACCACCGATGTGGTCCAACGCGGCAATATTGAAAAAGTGGTACTCACAAACGGCG
TGCTTTATCCGTCTAAATTAGTCAGTGTCGGCGCTCAAGTGTCGGGGTTAATCGAAACAATGGACGTGAATGTTGGCCAA
AACATAAAACAGGGCGACTTGATTGCTCAAATCGATAACTTAACTCAACAAAATGCCCTTAAAGAAGCCCAAGCATCATT
GCAAAGTATTGATGCGCAATATCGGGCAAAGCAGGCTCAAATAAAAGTCGCACAGTCTGAATATGCTCGTAATAAAAAGA
TGCTAGTTAATGGCGCTGCGTCGGTATCAGACTTTGACTCGGCAGAATCGATATTGGCGGTTTATCTGGCTGAGCTAGAT
GAACTCAGTGCTGAAAAAGACAAAGCAATCATCAGTGTCGATAGCGCCAAGCTCAATTTAGGTTACACAACTATTCGTTC
CCCCATTGATGGCACAGTGATTTACGTGTCGGTAGAAGAAGGGCAAACCGTTAATAACAATCAAGGTACCCCCAGTATTA
TTGAGCTAGCGCAATTGGATTTAATGACGATTAAGGCGCAGGTTTCTGAAGCTGACATTATCAATGTGAGTGCAGGGCAA
GCTGTTTATTTCAGTATTTTGGGGGCGACAGCGAAAAAGTACCGGGGTGTATTACGCACCATTGAACCAGGGCCAACATT
GCTTAGCGGGGACGACAGTTCGTTGTTGATTGGCGATGATGAGGCGATTTATTACAACGCTCTGTTTGATGTTGAAAACC
CAGGCAATCAGTTGCGTTTTGGCATGACGGCACAGGTATCGATTATTTTAGCCAGTGCGCAAGATGCGTTGCTTGTCCCA
TCACAGATATTGATAACAAAAACGGGGCCAAGTGTTTCGTATCAAGTGCCAGTGATAGTAGACAACCACATTGAATATCG
CGATGTTGAAGTGGGCATTAACAATAAAGTCTACGCTCAAATCCTCTCAGGGCTTAACGAAGGTGATCAAATCATGCTTG
GTCAAACTTCAGCTAATGACGATGCGATTTCTATGACCGGCTTATCTAAGGGCAATATGCTCGGACAAAGTGTACCAGGT
CTTGGTAAAGGAACGAGGTAA

Upstream 100 bases:

>100_bases
AATACGACTTCTGCTGTTATTGATCGCAAGTAAATGCAAAACCGTTATTACTAGATAATTGGCGCAAATTCAGTCACCGA
AAAGACATCAAGAGATCCGT

Downstream 100 bases:

>100_bases
TAACCCATGGGAGAAGTTCTGTTAAATATTGTGGATGTCAGCCGCTCTTTTATTGCCGGAGAGCAGGAGTTGACGGTACT
AAAAAACATTAATCTGCAAA

Product: RND family efflux transporter MFP subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 406; Mature: 406

Protein sequence:

>406_residues
MKKNIKNTLLLLILGATVAVVGYKYTQVQQDQPIFTTDVVQRGNIEKVVLTNGVLYPSKLVSVGAQVSGLIETMDVNVGQ
NIKQGDLIAQIDNLTQQNALKEAQASLQSIDAQYRAKQAQIKVAQSEYARNKKMLVNGAASVSDFDSAESILAVYLAELD
ELSAEKDKAIISVDSAKLNLGYTTIRSPIDGTVIYVSVEEGQTVNNNQGTPSIIELAQLDLMTIKAQVSEADIINVSAGQ
AVYFSILGATAKKYRGVLRTIEPGPTLLSGDDSSLLIGDDEAIYYNALFDVENPGNQLRFGMTAQVSIILASAQDALLVP
SQILITKTGPSVSYQVPVIVDNHIEYRDVEVGINNKVYAQILSGLNEGDQIMLGQTSANDDAISMTGLSKGNMLGQSVPG
LGKGTR

Sequences:

>Translated_406_residues
MKKNIKNTLLLLILGATVAVVGYKYTQVQQDQPIFTTDVVQRGNIEKVVLTNGVLYPSKLVSVGAQVSGLIETMDVNVGQ
NIKQGDLIAQIDNLTQQNALKEAQASLQSIDAQYRAKQAQIKVAQSEYARNKKMLVNGAASVSDFDSAESILAVYLAELD
ELSAEKDKAIISVDSAKLNLGYTTIRSPIDGTVIYVSVEEGQTVNNNQGTPSIIELAQLDLMTIKAQVSEADIINVSAGQ
AVYFSILGATAKKYRGVLRTIEPGPTLLSGDDSSLLIGDDEAIYYNALFDVENPGNQLRFGMTAQVSIILASAQDALLVP
SQILITKTGPSVSYQVPVIVDNHIEYRDVEVGINNKVYAQILSGLNEGDQIMLGQTSANDDAISMTGLSKGNMLGQSVPG
LGKGTR
>Mature_406_residues
MKKNIKNTLLLLILGATVAVVGYKYTQVQQDQPIFTTDVVQRGNIEKVVLTNGVLYPSKLVSVGAQVSGLIETMDVNVGQ
NIKQGDLIAQIDNLTQQNALKEAQASLQSIDAQYRAKQAQIKVAQSEYARNKKMLVNGAASVSDFDSAESILAVYLAELD
ELSAEKDKAIISVDSAKLNLGYTTIRSPIDGTVIYVSVEEGQTVNNNQGTPSIIELAQLDLMTIKAQVSEADIINVSAGQ
AVYFSILGATAKKYRGVLRTIEPGPTLLSGDDSSLLIGDDEAIYYNALFDVENPGNQLRFGMTAQVSIILASAQDALLVP
SQILITKTGPSVSYQVPVIVDNHIEYRDVEVGINNKVYAQILSGLNEGDQIMLGQTSANDDAISMTGLSKGNMLGQSVPG
LGKGTR

Specific function: Efflux transporter for macrolide antibiotics [H]

COG id: COG0845

COG function: function code M; Membrane-fusion protein

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI87081796, Length=343, Percent_Identity=34.1107871720117, Blast_Score=206, Evalue=2e-54,
Organism=Escherichia coli, GI1789929, Length=332, Percent_Identity=25, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1789637, Length=217, Percent_Identity=28.110599078341, Blast_Score=62, Evalue=5e-11,
Organism=Escherichia coli, GI1786668, Length=392, Percent_Identity=25.2551020408163, Blast_Score=62, Evalue=6e-11,
Organism=Escherichia coli, GI1790519, Length=266, Percent_Identity=24.812030075188, Blast_Score=62, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 43458; Mature: 43458

Theoretical pI: Translated: 4.46; Mature: 4.46

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKNIKNTLLLLILGATVAVVGYKYTQVQQDQPIFTTDVVQRGNIEKVVLTNGVLYPSKL
CCCCHHHHHHHHHHHHHHHHHCCEEEECCCCCCCEEHHHHCCCCCCEEEEECCCCCHHHH
VSVGAQVSGLIETMDVNVGQNIKQGDLIAQIDNLTQQNALKEAQASLQSIDAQYRAKQAQ
HHHCHHHHHHEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IKVAQSEYARNKKMLVNGAASVSDFDSAESILAVYLAELDELSAEKDKAIISVDSAKLNL
EEEHHHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEECCCEEEE
GYTTIRSPIDGTVIYVSVEEGQTVNNNQGTPSIIELAQLDLMTIKAQVSEADIINVSAGQ
CHHEECCCCCCEEEEEEECCCCEECCCCCCHHHHHHHHHHEEEEEEECCCCEEEEECCCC
AVYFSILGATAKKYRGVLRTIEPGPTLLSGDDSSLLIGDDEAIYYNALFDVENPGNQLRF
EEEEHHHHHHHHHHHHHHEECCCCCCEECCCCCEEEEECCCEEEEEEEEECCCCCCEEEE
GMTAQVSIILASAQDALLVPSQILITKTGPSVSYQVPVIVDNHIEYRDVEVGINNKVYAQ
CCEEEEEEEEECCCCCEECCCEEEEEECCCCCEEEEEEEEECCCEEEEEEECCCCHHHHH
ILSGLNEGDQIMLGQTSANDDAISMTGLSKGNMLGQSVPGLGKGTR
HHHCCCCCCEEEEECCCCCCCEEEEECCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MKKNIKNTLLLLILGATVAVVGYKYTQVQQDQPIFTTDVVQRGNIEKVVLTNGVLYPSKL
CCCCHHHHHHHHHHHHHHHHHCCEEEECCCCCCCEEHHHHCCCCCCEEEEECCCCCHHHH
VSVGAQVSGLIETMDVNVGQNIKQGDLIAQIDNLTQQNALKEAQASLQSIDAQYRAKQAQ
HHHCHHHHHHEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IKVAQSEYARNKKMLVNGAASVSDFDSAESILAVYLAELDELSAEKDKAIISVDSAKLNL
EEEHHHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEECCCEEEE
GYTTIRSPIDGTVIYVSVEEGQTVNNNQGTPSIIELAQLDLMTIKAQVSEADIINVSAGQ
CHHEECCCCCCEEEEEEECCCCEECCCCCCHHHHHHHHHHEEEEEEECCCCEEEEECCCC
AVYFSILGATAKKYRGVLRTIEPGPTLLSGDDSSLLIGDDEAIYYNALFDVENPGNQLRF
EEEEHHHHHHHHHHHHHHEECCCCCCEECCCCCEEEEECCCEEEEEEEEECCCCCCEEEE
GMTAQVSIILASAQDALLVPSQILITKTGPSVSYQVPVIVDNHIEYRDVEVGINNKVYAQ
CCEEEEEEEEECCCCCEECCCEEEEEECCCCCEEEEEEEEECCCEEEEEEECCCCHHHHH
ILSGLNEGDQIMLGQTSANDDAISMTGLSKGNMLGQSVPGLGKGTR
HHHCCCCCCEEEEECCCCCCCEEEEECCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11586360; 12142430 [H]