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The map label for this gene is pinE [C]

Identifier: 160873727

GI number: 160873727

Start: 722435

End: 722995

Strand: Direct

Name: pinE [C]

Synonym: Sbal195_0605

Alternate gene names: 160873727

Gene position: 722435-722995 (Clockwise)

Preceding gene: 160873726

Following gene: 160873728

Centisome position: 13.51

GC content: 58.47

Gene sequence:

>561_bases
ATGGTACTTTACGGCTATGCCCGCGTTTCAACCAGCGACCAGGATCTGACCCTGCAAACACAAATTCTCCGCGCTGCCGG
GTGTGAAATCATTCGTGTAGAAAAAGCCAGCGGAAACAGCCGGACCGGAAGGAGCGAGTTGCAGCTGCTGCTGGAGTTCC
TGCGCCCCGGTGATACGTTGATGGTGACCCGCGTGGATCGCCTGGCCCGCAGCATTAAGGACCTGCAGGATATCGTGTAT
GCACTGAATCAGCAGGGCGTAACGCTCAGGGCAACGGAACAGCCAGTGGACACGCGCTCGGCAGCAGGTAAAGGCTTCCT
CGATATGCTGGGTGTTTTCGCTGAGTTTGAAACCAACCTGCGACGTGAACACCAGATGGAAGGGATTGCCGCGGCGAAAG
CGCAGGGCGTGTACCGAGGAAGAAAGCCCTCAATAGACCCTGCAGAGGTCTGGCACCTGTACACCACGGAGAAGATGGGG
GCCACGGCCATTGCCCGTCAGCTCGGGATTGGGAGGGCATCAGTGTACCGGGCGCTGGAAAACTATGAGCAGACGGCGTA
G

Upstream 100 bases:

>100_bases
TCCGGTGAGACAGTTTTCCTGTCTCATTTGGGTTGTTTTTGTCTCATCCCGGTGTTACGTTTTTCATCATCTAGAGTTAA
ACGAGACAGTTTTCATTATT

Downstream 100 bases:

>100_bases
CGCGATTCCGCTTGATTCCCTGTTGCAACTAGGGCAACGGCTCGATCGCCTGCCGCCAAAAAGCCCTGAACGTGCCACCC
AGATCGCCGCTAGCGCTCAG

Product: resolvase domain-containing protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 186; Mature: 186

Protein sequence:

>186_residues
MVLYGYARVSTSDQDLTLQTQILRAAGCEIIRVEKASGNSRTGRSELQLLLEFLRPGDTLMVTRVDRLARSIKDLQDIVY
ALNQQGVTLRATEQPVDTRSAAGKGFLDMLGVFAEFETNLRREHQMEGIAAAKAQGVYRGRKPSIDPAEVWHLYTTEKMG
ATAIARQLGIGRASVYRALENYEQTA

Sequences:

>Translated_186_residues
MVLYGYARVSTSDQDLTLQTQILRAAGCEIIRVEKASGNSRTGRSELQLLLEFLRPGDTLMVTRVDRLARSIKDLQDIVY
ALNQQGVTLRATEQPVDTRSAAGKGFLDMLGVFAEFETNLRREHQMEGIAAAKAQGVYRGRKPSIDPAEVWHLYTTEKMG
ATAIARQLGIGRASVYRALENYEQTA
>Mature_186_residues
MVLYGYARVSTSDQDLTLQTQILRAAGCEIIRVEKASGNSRTGRSELQLLLEFLRPGDTLMVTRVDRLARSIKDLQDIVY
ALNQQGVTLRATEQPVDTRSAAGKGFLDMLGVFAEFETNLRREHQMEGIAAAKAQGVYRGRKPSIDPAEVWHLYTTEKMG
ATAIARQLGIGRASVYRALENYEQTA

Specific function: This Protein Catalyzes The Inversion Of An 1800-Bp E.Coli DNA Fragment, The P Region, Which Can Exist In Either Orientation. The Function Of The Inversion Is Not Yet Clear. [C]

COG id: COG1961

COG function: function code L; Site-specific recombinases, DNA invertase Pin homologs

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the site-specific recombinase resolvase family [H]

Homologues:

Organism=Escherichia coli, GI1787404, Length=150, Percent_Identity=40.6666666666667, Blast_Score=109, Evalue=1e-25,
Organism=Escherichia coli, GI1787827, Length=184, Percent_Identity=30.9782608695652, Blast_Score=75, Evalue=2e-15,
Organism=Escherichia coli, GI1787638, Length=184, Percent_Identity=30.9782608695652, Blast_Score=75, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR006118
- InterPro:   IPR006119
- InterPro:   IPR006120 [H]

Pfam domain/function: PF02796 HTH_7; PF00239 Resolvase [H]

EC number: NA

Molecular weight: Translated: 20665; Mature: 20665

Theoretical pI: Translated: 8.96; Mature: 8.96

Prosite motif: PS00397 RECOMBINASES_1 ; PS00398 RECOMBINASES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVLYGYARVSTSDQDLTLQTQILRAAGCEIIRVEKASGNSRTGRSELQLLLEFLRPGDTL
CEEEEEEEECCCCCCHHHHHHHHHHHCCEEEEEECCCCCCCCCHHHHHHHHHHHCCCCEE
MVTRVDRLARSIKDLQDIVYALNQQGVTLRATEQPVDTRSAAGKGFLDMLGVFAEFETNL
EHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHCCCCHHHHHHHHHHHHHHH
RREHQMEGIAAAKAQGVYRGRKPSIDPAEVWHLYTTEKMGATAIARQLGIGRASVYRALE
HHHHHHHHHHHHHHHHHCCCCCCCCCHHHHEEEHHHHHHHHHHHHHHHCCCHHHHHHHHH
NYEQTA
HHHCCC
>Mature Secondary Structure
MVLYGYARVSTSDQDLTLQTQILRAAGCEIIRVEKASGNSRTGRSELQLLLEFLRPGDTL
CEEEEEEEECCCCCCHHHHHHHHHHHCCEEEEEECCCCCCCCCHHHHHHHHHHHCCCCEE
MVTRVDRLARSIKDLQDIVYALNQQGVTLRATEQPVDTRSAAGKGFLDMLGVFAEFETNL
EHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHCCCCHHHHHHHHHHHHHHH
RREHQMEGIAAAKAQGVYRGRKPSIDPAEVWHLYTTEKMGATAIARQLGIGRASVYRALE
HHHHHHHHHHHHHHHHHCCCCCCCCCHHHHEEEHHHHHHHHHHHHHHHCCCHHHHHHHHH
NYEQTA
HHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9163424 [H]