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The map label for this gene is pmbA [H]

Identifier: 160873655

GI number: 160873655

Start: 615445

End: 616830

Strand: Direct

Name: pmbA [H]

Synonym: Sbal195_0533

Alternate gene names: 160873655

Gene position: 615445-616830 (Clockwise)

Preceding gene: 160873652

Following gene: 160873658

Centisome position: 11.51

GC content: 49.86

Gene sequence:

>1386_bases
TTGTTATGCTACGGCAATTGCCGACAACGAAAGAGTAGCTTTGTGTCTTTGAACAGAATCGATAGTGAATTGGGCGCGCT
GAAAGATGCGGTGGCCGTGGCGCTGGAATATGCCAACAAATTAGGAACGAATGCTGCCGAAGTGGCAATCAGTAAACAAC
AAGGTTTGTCGGTTTCTACCCGTTTAAAAGAAGTCGAAACCGTTGAATTTAATAAAGATGGTGCCTTAGGGATTACGGTT
TACCGCGATGGTTGTAAGGGCAGTTCATCGACGTCGGATTTAAGTCCAGAGGCTATCGCCTTAGCGGTGAAAGCGGCCGA
CGATATCGCCCGTTATACTTCTCCCGATCCATTCAGTGGTCTTGCCGATAAAGCCTTGATGGCAACTCAGATCCGCGATC
TTCAGCTGTATTATCCAGAAGATATTTCCCCCGATGAGTTAGCCCAACTGGCGATCCGCGCCGAAACTGCGGCACTCGAT
GCCGATCCGCGCATCAATAATTCCGATGGCGCCAGTGCCAATGCCCACACTGCGGTTAAAGTGTATGGCAACAGCCACGG
TTTCTTAAACGGGTATTGCAGCTCGCGTTATAGCTTAAGTTGCAGCGTGATCGGTGAAGATAGCGAAGGCAATATGCAAC
GGGATTACGATTACACTATCGCCCGTAAATTCAGCGAGATGCTTGCGCCTGAAACGGTCGGTTTAAAGACTGCCGAGAAG
ACAGTAAGCCGCCTCGGTGCGCGTAAGATTGCGACAACGCGTTTACCTATTTTATTAGCGCCAGATATCGCCACAGGGCT
AATCGGCCACTTCATCGGTGCGATCAGCGGTGGCAGTTTATACCGTAAATCCAGTTTCTTATTGGATTCGATCAACACCC
AAATCTTCCCCGATTGGTTCAATATCGAAGAACAGCCGCATTTACTGGGCGCACTGGCGAGCGCGAACTACGACAGCGAA
GGCGTGTCGACTCAAAATAGACGCATTATCGACCGCGGCATGTTAGAAACCTATTTGCTGACCAGCTACTCGGCGCGCAA
GTTAGGCTTAACCAACACGGGTCACGCGGGTGGTATTTATAACTGGACGCTCGGCCACACAGGCCAAACCTTCGACGAAT
TGGTAAAAGACATGGGCACTGGTTTGATTGTGACCGAAGTTATGGGCCAAGGCGTGAACATGGTTACGGGCGATTATTCC
CGTGGTGCGGCGGGTTTCTACGTTGAAAATGGTGAGATCCAATATCCAGTGGAAGAGATCACTATCGCGGGTAACTTAAA
AGAGATGTTCCGTGGCGTGCAGGCCGTGAGTAAGGATTTTGATCTGCGCTCATCGATTCGTACCGGCGGTATCTTGTTAT
CTGAAATGAAGATTGCGGGTAACTAA

Upstream 100 bases:

>100_bases
TAAACTCCCGCTGAATCCTGCATCTTCAGGTTGTTTGGGTATAGGATACGCTCTCAAGCCTAAGTCGGCCGCGAATAATT
AATGTGAATGGGATTTGTGT

Downstream 100 bases:

>100_bases
TTCATAGCTTCACTCGTTCAGCCGTTGCGCATAGTACGCTTAAGTCGTCTCAGCGTTTTAGTGCCTGTGCTTTAGCGCTA
GTAGCATGAATATGGTTTGA

Product: peptidase U62 modulator of DNA gyrase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 461; Mature: 461

Protein sequence:

>461_residues
MLCYGNCRQRKSSFVSLNRIDSELGALKDAVAVALEYANKLGTNAAEVAISKQQGLSVSTRLKEVETVEFNKDGALGITV
YRDGCKGSSSTSDLSPEAIALAVKAADDIARYTSPDPFSGLADKALMATQIRDLQLYYPEDISPDELAQLAIRAETAALD
ADPRINNSDGASANAHTAVKVYGNSHGFLNGYCSSRYSLSCSVIGEDSEGNMQRDYDYTIARKFSEMLAPETVGLKTAEK
TVSRLGARKIATTRLPILLAPDIATGLIGHFIGAISGGSLYRKSSFLLDSINTQIFPDWFNIEEQPHLLGALASANYDSE
GVSTQNRRIIDRGMLETYLLTSYSARKLGLTNTGHAGGIYNWTLGHTGQTFDELVKDMGTGLIVTEVMGQGVNMVTGDYS
RGAAGFYVENGEIQYPVEEITIAGNLKEMFRGVQAVSKDFDLRSSIRTGGILLSEMKIAGN

Sequences:

>Translated_461_residues
MLCYGNCRQRKSSFVSLNRIDSELGALKDAVAVALEYANKLGTNAAEVAISKQQGLSVSTRLKEVETVEFNKDGALGITV
YRDGCKGSSSTSDLSPEAIALAVKAADDIARYTSPDPFSGLADKALMATQIRDLQLYYPEDISPDELAQLAIRAETAALD
ADPRINNSDGASANAHTAVKVYGNSHGFLNGYCSSRYSLSCSVIGEDSEGNMQRDYDYTIARKFSEMLAPETVGLKTAEK
TVSRLGARKIATTRLPILLAPDIATGLIGHFIGAISGGSLYRKSSFLLDSINTQIFPDWFNIEEQPHLLGALASANYDSE
GVSTQNRRIIDRGMLETYLLTSYSARKLGLTNTGHAGGIYNWTLGHTGQTFDELVKDMGTGLIVTEVMGQGVNMVTGDYS
RGAAGFYVENGEIQYPVEEITIAGNLKEMFRGVQAVSKDFDLRSSIRTGGILLSEMKIAGN
>Mature_461_residues
MLCYGNCRQRKSSFVSLNRIDSELGALKDAVAVALEYANKLGTNAAEVAISKQQGLSVSTRLKEVETVEFNKDGALGITV
YRDGCKGSSSTSDLSPEAIALAVKAADDIARYTSPDPFSGLADKALMATQIRDLQLYYPEDISPDELAQLAIRAETAALD
ADPRINNSDGASANAHTAVKVYGNSHGFLNGYCSSRYSLSCSVIGEDSEGNMQRDYDYTIARKFSEMLAPETVGLKTAEK
TVSRLGARKIATTRLPILLAPDIATGLIGHFIGAISGGSLYRKSSFLLDSINTQIFPDWFNIEEQPHLLGALASANYDSE
GVSTQNRRIIDRGMLETYLLTSYSARKLGLTNTGHAGGIYNWTLGHTGQTFDELVKDMGTGLIVTEVMGQGVNMVTGDYS
RGAAGFYVENGEIQYPVEEITIAGNLKEMFRGVQAVSKDFDLRSSIRTGGILLSEMKIAGN

Specific function: May facilitate the secretion of the peptide antibiotic microcin B17 (MccB17) by completing its maturation. Suppresses the inhibitory activity of the carbon storage regulator (CsrA) [H]

COG id: COG0312

COG function: function code R; Predicted Zn-dependent proteases and their inactivated homologs

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase U62 family [H]

Homologues:

Organism=Escherichia coli, GI1790682, Length=445, Percent_Identity=59.3258426966292, Blast_Score=549, Evalue=1e-157,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002510 [H]

Pfam domain/function: PF01523 PmbA_TldD [H]

EC number: NA

Molecular weight: Translated: 49680; Mature: 49680

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLCYGNCRQRKSSFVSLNRIDSELGALKDAVAVALEYANKLGTNAAEVAISKQQGLSVST
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHEEEEHHCCCCHHH
RLKEVETVEFNKDGALGITVYRDGCKGSSSTSDLSPEAIALAVKAADDIARYTSPDPFSG
HHHHHEEEEECCCCCEEEEEEECCCCCCCCCCCCCCCCEEEEEHHHHHHHHCCCCCCCCC
LADKALMATQIRDLQLYYPEDISPDELAQLAIRAETAALDADPRINNSDGASANAHTAVK
HHHHHHHHHHHHEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEE
VYGNSHGFLNGYCSSRYSLSCSVIGEDSEGNMQRDYDYTIARKFSEMLAPETVGLKTAEK
EEECCCCCEEEECCCCEEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHCCCCCCCHHHHH
TVSRLGARKIATTRLPILLAPDIATGLIGHFIGAISGGSLYRKSSFLLDSINTQIFPDWF
HHHHHHHHHHHHCCCCEEECCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCHHH
NIEEQPHLLGALASANYDSEGVSTQNRRIIDRGMLETYLLTSYSARKLGLTNTGHAGGIY
CCCCCCHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEE
NWTLGHTGQTFDELVKDMGTGLIVTEVMGQGVNMVTGDYSRGAAGFYVENGEIQYPVEEI
EEECCCCCCHHHHHHHHHCCCEEEEHHHCCCCEEEECCCCCCCCEEEEECCEEECCHHHE
TIAGNLKEMFRGVQAVSKDFDLRSSIRTGGILLSEMKIAGN
EEECCHHHHHHHHHHHHHCCHHHHHHCCCCEEEEEEEECCC
>Mature Secondary Structure
MLCYGNCRQRKSSFVSLNRIDSELGALKDAVAVALEYANKLGTNAAEVAISKQQGLSVST
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHEEEEHHCCCCHHH
RLKEVETVEFNKDGALGITVYRDGCKGSSSTSDLSPEAIALAVKAADDIARYTSPDPFSG
HHHHHEEEEECCCCCEEEEEEECCCCCCCCCCCCCCCCEEEEEHHHHHHHHCCCCCCCCC
LADKALMATQIRDLQLYYPEDISPDELAQLAIRAETAALDADPRINNSDGASANAHTAVK
HHHHHHHHHHHHEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEE
VYGNSHGFLNGYCSSRYSLSCSVIGEDSEGNMQRDYDYTIARKFSEMLAPETVGLKTAEK
EEECCCCCEEEECCCCEEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHCCCCCCCHHHHH
TVSRLGARKIATTRLPILLAPDIATGLIGHFIGAISGGSLYRKSSFLLDSINTQIFPDWF
HHHHHHHHHHHHCCCCEEECCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCHHH
NIEEQPHLLGALASANYDSEGVSTQNRRIIDRGMLETYLLTSYSARKLGLTNTGHAGGIY
CCCCCCHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEE
NWTLGHTGQTFDELVKDMGTGLIVTEVMGQGVNMVTGDYSRGAAGFYVENGEIQYPVEEI
EEECCCCCCHHHHHHHHHCCCEEEEHHHCCCCEEEECCCCCCCCEEEEECCEEECCHHHE
TIAGNLKEMFRGVQAVSKDFDLRSSIRTGGILLSEMKIAGN
EEECCHHHHHHHHHHHHHCCHHHHHHCCCCEEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]