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The map label for this gene is leuD

Identifier: 160873525

GI number: 160873525

Start: 455181

End: 455786

Strand: Direct

Name: leuD

Synonym: Sbal195_0400

Alternate gene names: 160873525

Gene position: 455181-455786 (Clockwise)

Preceding gene: 160873524

Following gene: 160873526

Centisome position: 8.51

GC content: 48.84

Gene sequence:

>606_bases
ATGCAACCTTTTACGACCCATACCGGGCTTGCGGTCATGATCGACAGCACCAATATCGATACGGATCAGATCATTCCGAA
ACAGTTTTTATCTAAGGTGACGCGCGATGGTTTTGGCGTGCACTTATTTCACGACTGGCGTTATTTAGATGATGCGGGCG
ATCAGCCTAATCCTGAGTTTTCATTGAATCAAAGCCGTTATAAAGGCGCGTCGATTCTTTTGGCACAGGAAAACTTTGGT
TGTGGTTCAAGCCGCGAACATGCGCCTTGGGCGCTGGTAGACTTTGGTCTGCGTGCGATTATTGCGCCGAGTTTTGCTGA
CATTTTTTACGGTAACTCAATCAATAACGGCCTATTACCTGTGGCGTTAACCCACGCGCAGGTGCGCCAATTAATGGATG
AAGTGGCGGCAGAAGCTGGTGCACAAATTACGGTTGATCTTACAAGCTGCAAAGTGATTTCACCGTCGGGCGCCGAGTTT
AGTTTTACGCTGGCAGAATCGGCAAGGCATAAGTTGCTAAACGGTTTAGATGCGATTGGACTGACCTTGTCCCATGCTGC
GCAAATCAGTCAGTACGAAACCCAAATCCAAGGCTGGCGCCGTTAG

Upstream 100 bases:

>100_bases
TTTGGTGAGTCCTGCAATGGCTGCAGCCGCTGCGGTGGCAGGGCATTTTGTCGATATTCGTAAACCTTACTAATTACCGT
AAACCCAAGAGGAGAAGTTA

Downstream 100 bases:

>100_bases
TTTTTCAGCGCACATTTGTACTGGTGTTGAGTCCATATTTAAGCGCCTAGGTTAACACCTAGGCGCTTTTGTTTTAAATC
ATCATCTGCTCATGGTTTAA

Product: isopropylmalate isomerase small subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase

Number of amino acids: Translated: 201; Mature: 201

Protein sequence:

>201_residues
MQPFTTHTGLAVMIDSTNIDTDQIIPKQFLSKVTRDGFGVHLFHDWRYLDDAGDQPNPEFSLNQSRYKGASILLAQENFG
CGSSREHAPWALVDFGLRAIIAPSFADIFYGNSINNGLLPVALTHAQVRQLMDEVAAEAGAQITVDLTSCKVISPSGAEF
SFTLAESARHKLLNGLDAIGLTLSHAAQISQYETQIQGWRR

Sequences:

>Translated_201_residues
MQPFTTHTGLAVMIDSTNIDTDQIIPKQFLSKVTRDGFGVHLFHDWRYLDDAGDQPNPEFSLNQSRYKGASILLAQENFG
CGSSREHAPWALVDFGLRAIIAPSFADIFYGNSINNGLLPVALTHAQVRQLMDEVAAEAGAQITVDLTSCKVISPSGAEF
SFTLAESARHKLLNGLDAIGLTLSHAAQISQYETQIQGWRR
>Mature_201_residues
MQPFTTHTGLAVMIDSTNIDTDQIIPKQFLSKVTRDGFGVHLFHDWRYLDDAGDQPNPEFSLNQSRYKGASILLAQENFG
CGSSREHAPWALVDFGLRAIIAPSFADIFYGNSINNGLLPVALTHAQVRQLMDEVAAEAGAQITVDLTSCKVISPSGAEF
SFTLAESARHKLLNGLDAIGLTLSHAAQISQYETQIQGWRR

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate

COG id: COG0066

COG function: function code E; 3-isopropylmalate dehydratase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leuD family. LeuD type 1 subfamily

Homologues:

Organism=Escherichia coli, GI1786258, Length=194, Percent_Identity=58.7628865979381, Blast_Score=240, Evalue=6e-65,
Organism=Saccharomyces cerevisiae, GI6321429, Length=197, Percent_Identity=45.1776649746193, Blast_Score=174, Evalue=1e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LEUD_SHEB2 (B8E4K7)

Other databases:

- EMBL:   CP001252
- RefSeq:   YP_002356371.1
- GeneID:   7089552
- GenomeReviews:   CP001252_GR
- KEGG:   sbp:Sbal223_0414
- HOGENOM:   HBG304838
- OMA:   TVDLTTC
- ProtClustDB:   PRK01641
- HAMAP:   MF_01031
- InterPro:   IPR004431
- InterPro:   IPR012305
- InterPro:   IPR015937
- InterPro:   IPR015928
- InterPro:   IPR000573
- Gene3D:   G3DSA:3.20.19.10
- PANTHER:   PTHR11670:SF2
- PANTHER:   PTHR11670
- TIGRFAMs:   TIGR00171

Pfam domain/function: PF00694 Aconitase_C; SSF52016 Aconitase/3IPM_dehydase_swvl

EC number: =4.2.1.33

Molecular weight: Translated: 22034; Mature: 22034

Theoretical pI: Translated: 5.50; Mature: 5.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQPFTTHTGLAVMIDSTNIDTDQIIPKQFLSKVTRDGFGVHLFHDWRYLDDAGDQPNPEF
CCCCCCCCCEEEEEECCCCCHHHCCHHHHHHHHHCCCCEEEEEECEEEECCCCCCCCCCC
SLNQSRYKGASILLAQENFGCGSSREHAPWALVDFGLRAIIAPSFADIFYGNSINNGLLP
CCCCCCCCCEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHCCCCCCCCCHH
VALTHAQVRQLMDEVAAEAGAQITVDLTSCKVISPSGAEFSFTLAESARHKLLNGLDAIG
EEECHHHHHHHHHHHHHHCCCEEEEEECEEEEECCCCCEEEEEHHHHHHHHHHHHHHHHH
LTLSHAAQISQYETQIQGWRR
HHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MQPFTTHTGLAVMIDSTNIDTDQIIPKQFLSKVTRDGFGVHLFHDWRYLDDAGDQPNPEF
CCCCCCCCCEEEEEECCCCCHHHCCHHHHHHHHHCCCCEEEEEECEEEECCCCCCCCCCC
SLNQSRYKGASILLAQENFGCGSSREHAPWALVDFGLRAIIAPSFADIFYGNSINNGLLP
CCCCCCCCCEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHCCCCCCCCCHH
VALTHAQVRQLMDEVAAEAGAQITVDLTSCKVISPSGAEFSFTLAESARHKLLNGLDAIG
EEECHHHHHHHHHHHHHHCCCEEEEEECEEEEECCCCCEEEEEHHHHHHHHHHHHHHHHH
LTLSHAAQISQYETQIQGWRR
HHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA