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The map label for this gene is leuC [H]

Identifier: 160873524

GI number: 160873524

Start: 453747

End: 455153

Strand: Direct

Name: leuC [H]

Synonym: Sbal195_0399

Alternate gene names: 160873524

Gene position: 453747-455153 (Clockwise)

Preceding gene: 160873523

Following gene: 160873525

Centisome position: 8.49

GC content: 52.17

Gene sequence:

>1407_bases
ATGACGAACGCAAAGACCTTGTACCAAAAAGTATGGGATGCACATATCGTTGCTGCTCCCGAGGGCGAAGCGCCGGTCAT
TTATGTCGACAGACATTTAGTCCATGAAGTGACGTCACCTCAAGCATTCAGCGGTTTGAAAGTCGCGGGCCGTAAGCTGC
GCGCGCCGGAAAAAACCTTTGCGACTATGGATCATAATACTTCTACTCGCAGCGCCAGTTTAGATGCCCTAAGCCCGATG
GCGCGCACTCAGGTGGAAACCCTAGCGCAAAACTGTAAAGACTTTGGCGTGCGCTTATATGATATTCACCATCCTAATCA
AGGGATTGTGCATGTGATGGGCCCTGAGCTAGGCATTACCTTACCCGGCACTGTGATTGTGTGTGGCGATTCCCATACCG
CCACCCACGGCGCCTTTGGCGCATTGGCGTTTGGGATTGGCACTTCGGAAGTTGAGCACGTGTTAGCAACGCAAACGCTG
CGTCAGTTAAAAGCTAAAACCATGAAGATTGAAGTGCGTGGTCATGTCACCGATGGCGTGACCGCCAAAGATATCGTCCT
CGCTATTATTGGCAAAATCGGCATGGATGGTGGCACGGGTTACGTGGTGGAGTTCTGCGGTGAGGCGATCGAAGCCCTGT
CGATGGAAGGCCGCATGACAGTGTGTAACATGGCGATTGAGATGGGGGCTAAGGCGGGCATGGTCGCGCCGGATCAAACC
ACTTTCGATTATTTAGCAGGCCGCGAGTTTGCGCCAAAGGGCGAAGATTGGGCAGAGGCTGTCGCTTACTGGAAAGCCAT
TAAAACCGATGACGGCGCCGTGTTTGATGCTATCGTTGAGTTAGATGCTGCCGATATTGCGCCACAATTGACTTGGGGTA
CTAACCCAGGCCAAGTGGTGGCAATCGATGGCAAAGTACCTGACCCCATTAATGAAGCCAACCCAAGCACCCGAGCTAGT
ATGGAAAAGGCTTTAGAATACATTGGCCTGAGTGCGGGTACGCCGATGACAGATATCAGTATCAACAAGGTGTTTATCGG
TTCATGTACTAACTCACGGATTGAAGATTTACGCAGCGCCGCTGTACATGCCAAGGGCCGTAAAGTCGCGAGTGGCGTCA
CCGCCATTGTCGTTCCAGGTTCTGGCCAAGTGAAAGCGCAAGCCGAAGCCGAAGGCTTAGATAAAATCTTTATCGAAGCG
GGATTTGAGTGGCGTTTACCGGGTTGTTCTATGTGTTTAGCGATGAACGATGACAGATTAGAAGCGGGCGATCGCTGCGC
GTCGACCAGTAATCGTAACTTCGAAGGTCGCCAAGGTCGTGGCAGTCGTACCCATTTGGTGAGTCCTGCAATGGCTGCAG
CCGCTGCGGTGGCAGGGCATTTTGTCGATATTCGTAAACCTTACTAA

Upstream 100 bases:

>100_bases
ATTTAACTGGCGAGCTGTTGAGCAGCGACAAGCGTAGCGAGGCTAAATCGACGGCAGAAATGGGTGACTTTATCGCCAAT
GCTATAAAGGAAGGTGTGTA

Downstream 100 bases:

>100_bases
TTACCGTAAACCCAAGAGGAGAAGTTAATGCAACCTTTTACGACCCATACCGGGCTTGCGGTCATGATCGACAGCACCAA
TATCGATACGGATCAGATCA

Product: isopropylmalate isomerase large subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase [H]

Number of amino acids: Translated: 468; Mature: 467

Protein sequence:

>468_residues
MTNAKTLYQKVWDAHIVAAPEGEAPVIYVDRHLVHEVTSPQAFSGLKVAGRKLRAPEKTFATMDHNTSTRSASLDALSPM
ARTQVETLAQNCKDFGVRLYDIHHPNQGIVHVMGPELGITLPGTVIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTL
RQLKAKTMKIEVRGHVTDGVTAKDIVLAIIGKIGMDGGTGYVVEFCGEAIEALSMEGRMTVCNMAIEMGAKAGMVAPDQT
TFDYLAGREFAPKGEDWAEAVAYWKAIKTDDGAVFDAIVELDAADIAPQLTWGTNPGQVVAIDGKVPDPINEANPSTRAS
MEKALEYIGLSAGTPMTDISINKVFIGSCTNSRIEDLRSAAVHAKGRKVASGVTAIVVPGSGQVKAQAEAEGLDKIFIEA
GFEWRLPGCSMCLAMNDDRLEAGDRCASTSNRNFEGRQGRGSRTHLVSPAMAAAAAVAGHFVDIRKPY

Sequences:

>Translated_468_residues
MTNAKTLYQKVWDAHIVAAPEGEAPVIYVDRHLVHEVTSPQAFSGLKVAGRKLRAPEKTFATMDHNTSTRSASLDALSPM
ARTQVETLAQNCKDFGVRLYDIHHPNQGIVHVMGPELGITLPGTVIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTL
RQLKAKTMKIEVRGHVTDGVTAKDIVLAIIGKIGMDGGTGYVVEFCGEAIEALSMEGRMTVCNMAIEMGAKAGMVAPDQT
TFDYLAGREFAPKGEDWAEAVAYWKAIKTDDGAVFDAIVELDAADIAPQLTWGTNPGQVVAIDGKVPDPINEANPSTRAS
MEKALEYIGLSAGTPMTDISINKVFIGSCTNSRIEDLRSAAVHAKGRKVASGVTAIVVPGSGQVKAQAEAEGLDKIFIEA
GFEWRLPGCSMCLAMNDDRLEAGDRCASTSNRNFEGRQGRGSRTHLVSPAMAAAAAVAGHFVDIRKPY
>Mature_467_residues
TNAKTLYQKVWDAHIVAAPEGEAPVIYVDRHLVHEVTSPQAFSGLKVAGRKLRAPEKTFATMDHNTSTRSASLDALSPMA
RTQVETLAQNCKDFGVRLYDIHHPNQGIVHVMGPELGITLPGTVIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLR
QLKAKTMKIEVRGHVTDGVTAKDIVLAIIGKIGMDGGTGYVVEFCGEAIEALSMEGRMTVCNMAIEMGAKAGMVAPDQTT
FDYLAGREFAPKGEDWAEAVAYWKAIKTDDGAVFDAIVELDAADIAPQLTWGTNPGQVVAIDGKVPDPINEANPSTRASM
EKALEYIGLSAGTPMTDISINKVFIGSCTNSRIEDLRSAAVHAKGRKVASGVTAIVVPGSGQVKAQAEAEGLDKIFIEAG
FEWRLPGCSMCLAMNDDRLEAGDRCASTSNRNFEGRQGRGSRTHLVSPAMAAAAAVAGHFVDIRKPY

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate [H]

COG id: COG0065

COG function: function code E; 3-isopropylmalate dehydratase large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family. LeuC type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI4501867, Length=370, Percent_Identity=29.1891891891892, Blast_Score=116, Evalue=4e-26,
Organism=Homo sapiens, GI8659555, Length=401, Percent_Identity=27.431421446384, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI41352693, Length=381, Percent_Identity=26.7716535433071, Blast_Score=91, Evalue=2e-18,
Organism=Escherichia coli, GI1786259, Length=463, Percent_Identity=73.2181425485961, Blast_Score=709, Evalue=0.0,
Organism=Escherichia coli, GI1787531, Length=367, Percent_Identity=29.1553133514986, Blast_Score=102, Evalue=6e-23,
Organism=Escherichia coli, GI87081781, Length=342, Percent_Identity=25.1461988304094, Blast_Score=74, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI25149337, Length=383, Percent_Identity=28.7206266318538, Blast_Score=133, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI32564738, Length=383, Percent_Identity=28.7206266318538, Blast_Score=132, Evalue=3e-31,
Organism=Caenorhabditis elegans, GI25149342, Length=305, Percent_Identity=28.8524590163934, Blast_Score=122, Evalue=5e-28,
Organism=Caenorhabditis elegans, GI17568399, Length=441, Percent_Identity=27.6643990929705, Blast_Score=104, Evalue=8e-23,
Organism=Saccharomyces cerevisiae, GI6321429, Length=469, Percent_Identity=62.2601279317697, Blast_Score=581, Evalue=1e-167,
Organism=Saccharomyces cerevisiae, GI6320440, Length=462, Percent_Identity=26.8398268398268, Blast_Score=150, Evalue=5e-37,
Organism=Saccharomyces cerevisiae, GI6323335, Length=370, Percent_Identity=29.4594594594595, Blast_Score=139, Evalue=9e-34,
Organism=Saccharomyces cerevisiae, GI6322261, Length=398, Percent_Identity=27.8894472361809, Blast_Score=132, Evalue=1e-31,
Organism=Drosophila melanogaster, GI281365315, Length=383, Percent_Identity=27.154046997389, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI17864292, Length=383, Percent_Identity=27.154046997389, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI161076999, Length=391, Percent_Identity=26.5984654731458, Blast_Score=124, Evalue=2e-28,
Organism=Drosophila melanogaster, GI28571643, Length=383, Percent_Identity=27.154046997389, Blast_Score=107, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24645686, Length=458, Percent_Identity=25.9825327510917, Blast_Score=78, Evalue=1e-14,
Organism=Drosophila melanogaster, GI17137564, Length=423, Percent_Identity=26.0047281323877, Blast_Score=76, Evalue=5e-14,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004430
- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015932
- InterPro:   IPR018136
- InterPro:   IPR015936 [H]

Pfam domain/function: PF00330 Aconitase [H]

EC number: =4.2.1.33 [H]

Molecular weight: Translated: 49761; Mature: 49630

Theoretical pI: Translated: 6.15; Mature: 6.15

Prosite motif: PS00450 ACONITASE_1 ; PS01244 ACONITASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNAKTLYQKVWDAHIVAAPEGEAPVIYVDRHLVHEVTSPQAFSGLKVAGRKLRAPEKTF
CCCHHHHHHHHCCCEEEECCCCCCCEEEECHHHHHHCCCCCHHCCHHHHHHHCCCCCHHH
ATMDHNTSTRSASLDALSPMARTQVETLAQNCKDFGVRLYDIHHPNQGIVHVMGPELGIT
EECCCCCCCCCCCHHHHCHHHHHHHHHHHHHHHHHCEEEEEEECCCCCEEEEECCCCCEE
LPGTVIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLRQLKAKTMKIEVRGHVTDGV
CCCEEEEECCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHEEEEEEEEECCCCC
TAKDIVLAIIGKIGMDGGTGYVVEFCGEAIEALSMEGRMTVCNMAIEMGAKAGMVAPDQT
CHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCCCC
TFDYLAGREFAPKGEDWAEAVAYWKAIKTDDGAVFDAIVELDAADIAPQLTWGTNPGQVV
HHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHCCHHHCCCCEEECCCCCEEE
AIDGKVPDPINEANPSTRASMEKALEYIGLSAGTPMTDISINKVFIGSCTNSRIEDLRSA
EECCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCEEEECEEEEECCCCHHHHHHHHH
AVHAKGRKVASGVTAIVVPGSGQVKAQAEAEGLDKIFIEAGFEWRLPGCSMCLAMNDDRL
HHHHCCCEECCCCEEEEECCCCCEEECHHHCCCCEEEEECCCEEECCCCEEEEEECCCCC
EAGDRCASTSNRNFEGRQGRGSRTHLVSPAMAAAAAVAGHFVDIRKPY
CCCCHHCCCCCCCCCCCCCCCCCCEECCHHHHHHHHHHHHEEEECCCC
>Mature Secondary Structure 
TNAKTLYQKVWDAHIVAAPEGEAPVIYVDRHLVHEVTSPQAFSGLKVAGRKLRAPEKTF
CCHHHHHHHHCCCEEEECCCCCCCEEEECHHHHHHCCCCCHHCCHHHHHHHCCCCCHHH
ATMDHNTSTRSASLDALSPMARTQVETLAQNCKDFGVRLYDIHHPNQGIVHVMGPELGIT
EECCCCCCCCCCCHHHHCHHHHHHHHHHHHHHHHHCEEEEEEECCCCCEEEEECCCCCEE
LPGTVIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLRQLKAKTMKIEVRGHVTDGV
CCCEEEEECCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHEEEEEEEEECCCCC
TAKDIVLAIIGKIGMDGGTGYVVEFCGEAIEALSMEGRMTVCNMAIEMGAKAGMVAPDQT
CHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCCCC
TFDYLAGREFAPKGEDWAEAVAYWKAIKTDDGAVFDAIVELDAADIAPQLTWGTNPGQVV
HHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHCCHHHCCCCEEECCCCCEEE
AIDGKVPDPINEANPSTRASMEKALEYIGLSAGTPMTDISINKVFIGSCTNSRIEDLRSA
EECCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCEEEECEEEEECCCCHHHHHHHHH
AVHAKGRKVASGVTAIVVPGSGQVKAQAEAEGLDKIFIEAGFEWRLPGCSMCLAMNDDRL
HHHHCCCEECCCCEEEEECCCCCEEECHHHCCCCEEEEECCCEEECCCCEEEEEECCCCC
EAGDRCASTSNRNFEGRQGRGSRTHLVSPAMAAAAAVAGHFVDIRKPY
CCCCHHCCCCCCCCCCCCCCCCCCEECCHHHHHHHHHHHHEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12368813 [H]