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The map label for this gene is rph

Identifier: 160873506

GI number: 160873506

Start: 434153

End: 434866

Strand: Direct

Name: rph

Synonym: Sbal195_0381

Alternate gene names: 160873506

Gene position: 434153-434866 (Clockwise)

Preceding gene: 160873504

Following gene: 160873507

Centisome position: 8.12

GC content: 51.26

Gene sequence:

>714_bases
ATGCGTCCAAGTAACAGAACGCCAGCACAAACTCGTCCCATCACTATCACTCGCCAGTTTACGGCCCATGCCGAAGGTTC
TGTGTTAGTGGAATTTGGCGAAACGAAAGTGCTTTGTACCGCCAGTTTTACTGAAGGTGTGCCACGTTTCCTTAAAGGTC
AGGGTCAAGGCTGGGTGACGGCGGAATACGGCATGTTGCCACGTTCGACCCATAGCCGTATGGATCGTGAAGCCGCTCGC
GGTAAGCAATCTGGCCGTACGCAAGAAATTCAACGTCTTATCGGCCGTGCTCTGCGCGCTTGTGTAGATATGAAAGCCCT
AGGCGAAAACACTATCGTGATCGACTGTGATGTGATCCAAGCTGACGGTGGCACGCGCACTGCCTCTATCACTGGCGCCT
GTGTGGCCTTAGTGGATGCGTTGAACTGGGCGCGTGGTAAAGGCATCATTAAATCTAACCCGCTGAAATTCCTTATCGCT
GCTGTCAGCGTAGGTATTTACAAGGGTGAAGCGATCAGCGATCTAGAATATATCGAAGACAGCGCCGCTGAAACTGACAT
GAACGTGGTGATGACGGAAACGGGAAAGATCATCGAAATTCAAGGCACCGCCGAAGGCGAGCCATTCAGCCATGAAGAAT
TACTCGAACTGTTGGCATTGGCGAAGAACAGTATTCGTGAAATCGTCGATGTGCAGAAAGCCGCACTGAACTAA

Upstream 100 bases:

>100_bases
GAGTTTGCCAGAGAATCACCCTAAGCATGGCATCCTAAGGCTCAAGCCCTTATAATATGCAGCCAAAATCGGTCAATGAC
TCAGAATACAGGAATCTCGC

Downstream 100 bases:

>100_bases
TAGTGTGGTGATGAAAAAGCCCCGTCATGTTATAGAGTGCGGGGCTTTTTATTAGCTGCAACTTTTTATCTGCAGCAAGA
TGCTAGTGTTAGACAAGCCC

Product: ribonuclease PH

Products: NA

Alternate protein names: RNase PH; tRNA nucleotidyltransferase

Number of amino acids: Translated: 237; Mature: 237

Protein sequence:

>237_residues
MRPSNRTPAQTRPITITRQFTAHAEGSVLVEFGETKVLCTASFTEGVPRFLKGQGQGWVTAEYGMLPRSTHSRMDREAAR
GKQSGRTQEIQRLIGRALRACVDMKALGENTIVIDCDVIQADGGTRTASITGACVALVDALNWARGKGIIKSNPLKFLIA
AVSVGIYKGEAISDLEYIEDSAAETDMNVVMTETGKIIEIQGTAEGEPFSHEELLELLALAKNSIREIVDVQKAALN

Sequences:

>Translated_237_residues
MRPSNRTPAQTRPITITRQFTAHAEGSVLVEFGETKVLCTASFTEGVPRFLKGQGQGWVTAEYGMLPRSTHSRMDREAAR
GKQSGRTQEIQRLIGRALRACVDMKALGENTIVIDCDVIQADGGTRTASITGACVALVDALNWARGKGIIKSNPLKFLIA
AVSVGIYKGEAISDLEYIEDSAAETDMNVVMTETGKIIEIQGTAEGEPFSHEELLELLALAKNSIREIVDVQKAALN
>Mature_237_residues
MRPSNRTPAQTRPITITRQFTAHAEGSVLVEFGETKVLCTASFTEGVPRFLKGQGQGWVTAEYGMLPRSTHSRMDREAAR
GKQSGRTQEIQRLIGRALRACVDMKALGENTIVIDCDVIQADGGTRTASITGACVALVDALNWARGKGIIKSNPLKFLIA
AVSVGIYKGEAISDLEYIEDSAAETDMNVVMTETGKIIEIQGTAEGEPFSHEELLELLALAKNSIREIVDVQKAALN

Specific function: Phosphorolytic exoribonuclease that removes nucleotide residues following the -CCA terminus of tRNA and adds nucleotides to the ends of RNA molecules by using nucleoside diphosphates as substrates

COG id: COG0689

COG function: function code J; RNase PH

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNase PH family

Homologues:

Organism=Escherichia coli, GI157672248, Length=211, Percent_Identity=76.303317535545, Blast_Score=341, Evalue=3e-95,
Organism=Caenorhabditis elegans, GI71981632, Length=187, Percent_Identity=29.9465240641711, Blast_Score=67, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RNPH_SHEB2 (B8E4I8)

Other databases:

- EMBL:   CP001252
- RefSeq:   YP_002356352.1
- ProteinModelPortal:   B8E4I8
- SMR:   B8E4I8
- GeneID:   7089533
- GenomeReviews:   CP001252_GR
- KEGG:   sbp:Sbal223_0395
- HOGENOM:   HBG737187
- OMA:   MLPRATG
- ProtClustDB:   PRK00173
- HAMAP:   MF_00564
- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR020568
- InterPro:   IPR002381
- InterPro:   IPR018336
- TIGRFAMs:   TIGR01966

Pfam domain/function: PF01138 RNase_PH; PF03725 RNase_PH_C; SSF55666 3_ExoRNase; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =2.7.7.56

Molecular weight: Translated: 25649; Mature: 25649

Theoretical pI: Translated: 5.81; Mature: 5.81

Prosite motif: PS01277 RIBONUCLEASE_PH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRPSNRTPAQTRPITITRQFTAHAEGSVLVEFGETKVLCTASFTEGVPRFLKGQGQGWVT
CCCCCCCCCCCCCEEEEEEEECCCCCCEEEEECCEEEEEEECHHHCCHHHHCCCCCCEEE
AEYGMLPRSTHSRMDREAARGKQSGRTQEIQRLIGRALRACVDMKALGENTIVIDCDVIQ
EECCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEE
ADGGTRTASITGACVALVDALNWARGKGIIKSNPLKFLIAAVSVGIYKGEAISDLEYIED
CCCCCEEEHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHH
SAAETDMNVVMTETGKIIEIQGTAEGEPFSHEELLELLALAKNSIREIVDVQKAALN
CCCCCCCEEEEECCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MRPSNRTPAQTRPITITRQFTAHAEGSVLVEFGETKVLCTASFTEGVPRFLKGQGQGWVT
CCCCCCCCCCCCCEEEEEEEECCCCCCEEEEECCEEEEEEECHHHCCHHHHCCCCCCEEE
AEYGMLPRSTHSRMDREAARGKQSGRTQEIQRLIGRALRACVDMKALGENTIVIDCDVIQ
EECCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEE
ADGGTRTASITGACVALVDALNWARGKGIIKSNPLKFLIAAVSVGIYKGEAISDLEYIED
CCCCCEEEHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHH
SAAETDMNVVMTETGKIIEIQGTAEGEPFSHEELLELLALAKNSIREIVDVQKAALN
CCCCCCCEEEEECCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA