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The map label for this gene is glnA [H]

Identifier: 160873436

GI number: 160873436

Start: 353346

End: 354755

Strand: Reverse

Name: glnA [H]

Synonym: Sbal195_0311

Alternate gene names: 160873436

Gene position: 354755-353346 (Counterclockwise)

Preceding gene: 160873438

Following gene: 160873432

Centisome position: 6.63

GC content: 47.3

Gene sequence:

>1410_bases
ATGTCAGTTGAATCAGTTTTAAAGCAACTTGAAGAATTAGAAGTTAAGTTCGTTGATTTACGTTTTACCGACACTAAAGG
TAAAGAGCAGCACGTATCTATTCCTTCTCATCAGGTAGATGCTGATTTCTTCGAAGACGGCAAAATGTTTGACGGTTCTT
CTATCGCAGGTTGGAAGGGCATTAACGAATCAGACATGGTACTGATGCCAGATCCAACGACATTCGTACTGGATCCTTTC
ACCGAAGAAACCACAGCGCTTATTCGTTGTGACATTCTTGAACCAGGTACTATGACGGGCTATGACCGTGACCCACGTTC
AATTGCTAAAAAAGCAGAAGCTTACCTAATCTCTACTGGTATCGCTGATACTGTTTTGATTGGCCCAGAACCAGAATTCT
TCCTATTTGATGATGTCCGTTTCGGTACTGACATGTCAGGTTGTTTCGTTAAGATCGACGCTAAAGAAGCGGCTTGGAAC
TCAGGCACGAGCTACGAAGGCGGCAACACAGGTCACCGTCCATTCACTAAAGGCGGTTACTTCCCAGTTGCACCGGTTGA
CTCATCACAAGATCTACGTAGCGCTATGTGTTTAGTGCTTGAAGAAATGGGCCAAGTGGTTGAAGCGCATCACCACGAAG
TGGCGACTGCGGGTCAAAACGAAATCGCAACTCGCTTCAACACGCTAACTAAGAAAGCGGATGAAATCCAAATTCTGAAG
TACGTAGTGCACAACATGGCACACGCTTACGGTAAGACGGCGACTTTCATGCCTAAACCCATCGTTGGCGACAACGGTAG
CGGCATGCACGTTCACCAATCTCTGGCGAAAGACGGTGTTAACTTGTTCGCTGGCGACAAATACGCGGGTTTAAGTGAAA
CGGCTCTGTACTACATAGGTGGTATCATCAAGCACGCTCGCGCGCTGAACGCTTTCACTAACCCAAGCACTAACTCTTAC
AAGCGTTTAGTGCCACACTTTGAAGCACCTGTTATGTTGGCCTACTCTGCCCGTAACCGCTCTGCATCAATCCGTATTCC
AGTGGTACCAAGCCCTAAAGGCCGTCGTATCGAAGCGCGTTTCCCAGATCCACATGCCAACCCATACTTAGGTTTCGCAG
CGCTGCTGATGGCCGGTCTTGACGGTATCCAAAACAAGATCCACCCAGGTGAAGCGATGGACAAAGATCTGTACGATCTG
CCTCCAGAAGAAGCGGCTGAAATCCCACAAGTGGCAACGTCATTAGAAAACGCCCTTGAAAATCTGCAAGCTGACCATGA
GTTCCTGACAAAAGGCGGCGTGTTCAGTGAAGATTTCATCCAATCTTATATCGTACTGAAAACAGCTGAAGCTGAGCGTG
TAGCACGTACAACTCACCCACTTGAGTTTGAAATGTACTACAGCTTGTAA

Upstream 100 bases:

>100_bases
ATAGATATCGCTTTATCGCTCGTAAGTTTGGCTCAAGATAAATCCCTTGAGTTAAAAGATTATAGAAGGGGCATCCCTTT
ACCCTACCGGAGGCTTGAGA

Downstream 100 bases:

>100_bases
TCCTTACTCGCAAATTGATTAAGCCCCGAATTGTCGGGGCTTTTTTATGCCTAGCGGGGCATCTATGACGATTGAATTAT
TTTATGTAATTAACTGAATA

Product: glutamine synthetase

Products: NA

Alternate protein names: Glutamate--ammonia ligase [H]

Number of amino acids: Translated: 469; Mature: 468

Protein sequence:

>469_residues
MSVESVLKQLEELEVKFVDLRFTDTKGKEQHVSIPSHQVDADFFEDGKMFDGSSIAGWKGINESDMVLMPDPTTFVLDPF
TEETTALIRCDILEPGTMTGYDRDPRSIAKKAEAYLISTGIADTVLIGPEPEFFLFDDVRFGTDMSGCFVKIDAKEAAWN
SGTSYEGGNTGHRPFTKGGYFPVAPVDSSQDLRSAMCLVLEEMGQVVEAHHHEVATAGQNEIATRFNTLTKKADEIQILK
YVVHNMAHAYGKTATFMPKPIVGDNGSGMHVHQSLAKDGVNLFAGDKYAGLSETALYYIGGIIKHARALNAFTNPSTNSY
KRLVPHFEAPVMLAYSARNRSASIRIPVVPSPKGRRIEARFPDPHANPYLGFAALLMAGLDGIQNKIHPGEAMDKDLYDL
PPEEAAEIPQVATSLENALENLQADHEFLTKGGVFSEDFIQSYIVLKTAEAERVARTTHPLEFEMYYSL

Sequences:

>Translated_469_residues
MSVESVLKQLEELEVKFVDLRFTDTKGKEQHVSIPSHQVDADFFEDGKMFDGSSIAGWKGINESDMVLMPDPTTFVLDPF
TEETTALIRCDILEPGTMTGYDRDPRSIAKKAEAYLISTGIADTVLIGPEPEFFLFDDVRFGTDMSGCFVKIDAKEAAWN
SGTSYEGGNTGHRPFTKGGYFPVAPVDSSQDLRSAMCLVLEEMGQVVEAHHHEVATAGQNEIATRFNTLTKKADEIQILK
YVVHNMAHAYGKTATFMPKPIVGDNGSGMHVHQSLAKDGVNLFAGDKYAGLSETALYYIGGIIKHARALNAFTNPSTNSY
KRLVPHFEAPVMLAYSARNRSASIRIPVVPSPKGRRIEARFPDPHANPYLGFAALLMAGLDGIQNKIHPGEAMDKDLYDL
PPEEAAEIPQVATSLENALENLQADHEFLTKGGVFSEDFIQSYIVLKTAEAERVARTTHPLEFEMYYSL
>Mature_468_residues
SVESVLKQLEELEVKFVDLRFTDTKGKEQHVSIPSHQVDADFFEDGKMFDGSSIAGWKGINESDMVLMPDPTTFVLDPFT
EETTALIRCDILEPGTMTGYDRDPRSIAKKAEAYLISTGIADTVLIGPEPEFFLFDDVRFGTDMSGCFVKIDAKEAAWNS
GTSYEGGNTGHRPFTKGGYFPVAPVDSSQDLRSAMCLVLEEMGQVVEAHHHEVATAGQNEIATRFNTLTKKADEIQILKY
VVHNMAHAYGKTATFMPKPIVGDNGSGMHVHQSLAKDGVNLFAGDKYAGLSETALYYIGGIIKHARALNAFTNPSTNSYK
RLVPHFEAPVMLAYSARNRSASIRIPVVPSPKGRRIEARFPDPHANPYLGFAALLMAGLDGIQNKIHPGEAMDKDLYDLP
PEEAAEIPQVATSLENALENLQADHEFLTKGGVFSEDFIQSYIVLKTAEAERVARTTHPLEFEMYYSL

Specific function: Unknown

COG id: COG0174

COG function: function code E; Glutamine synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine synthetase family [H]

Homologues:

Organism=Escherichia coli, GI1790301, Length=469, Percent_Identity=77.3987206823028, Blast_Score=768, Evalue=0.0,
Organism=Escherichia coli, GI87081870, Length=288, Percent_Identity=27.4305555555556, Blast_Score=82, Evalue=7e-17,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 15324 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 8,000 Molecules/Cell In: Glucose minimal medi

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008147
- InterPro:   IPR014746
- InterPro:   IPR008146
- InterPro:   IPR004809
- InterPro:   IPR001637 [H]

Pfam domain/function: PF00120 Gln-synt_C; PF03951 Gln-synt_N [H]

EC number: =6.3.1.2 [H]

Molecular weight: Translated: 51697; Mature: 51566

Theoretical pI: Translated: 4.92; Mature: 4.92

Prosite motif: PS00180 GLNA_1 ; PS00181 GLNA_ATP ; PS00182 GLNA_ADENYLATION

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVESVLKQLEELEVKFVDLRFTDTKGKEQHVSIPSHQVDADFFEDGKMFDGSSIAGWKG
CCHHHHHHHHHHCCEEEEEEEEECCCCCCCEECCCCCCCCCHHHCCCCEECCCCCCCCCC
INESDMVLMPDPTTFVLDPFTEETTALIRCDILEPGTMTGYDRDPRSIAKKAEAYLISTG
CCCCCEEEECCCCEEEECCCCCCCEEEEEEEECCCCCCCCCCCCHHHHHHHHHHEEEECC
IADTVLIGPEPEFFLFDDVRFGTDMSGCFVKIDAKEAAWNSGTSYEGGNTGHRPFTKGGY
CCCEEEECCCCCEEEEECEECCCCCCCEEEEEECHHHCCCCCCCCCCCCCCCCCCCCCCC
FPVAPVDSSQDLRSAMCLVLEEMGQVVEAHHHEVATAGQNEIATRFNTLTKKADEIQILK
EEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
YVVHNMAHAYGKTATFMPKPIVGDNGSGMHVHQSLAKDGVNLFAGDKYAGLSETALYYIG
HHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCEEEECCCCCCCHHHHHHHHH
GIIKHARALNAFTNPSTNSYKRLVPHFEAPVMLAYSARNRSASIRIPVVPSPKGRRIEAR
HHHHHHHHHHHCCCCCCCCHHHHCCCCCCCEEEEEECCCCCCEEEEEECCCCCCCEEEEE
FPDPHANPYLGFAALLMAGLDGIQNKIHPGEAMDKDLYDLPPEEAAEIPQVATSLENALE
CCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHCCCHHCCCCHHHHHHHHHHHHHHHHHH
NLQADHEFLTKGGVFSEDFIQSYIVLKTAEAERVARTTHPLEFEMYYSL
HHHHHHHHHHCCCCCCHHHHHHHHEEEECCHHHHHHHCCCEEEEEEECC
>Mature Secondary Structure 
SVESVLKQLEELEVKFVDLRFTDTKGKEQHVSIPSHQVDADFFEDGKMFDGSSIAGWKG
CHHHHHHHHHHCCEEEEEEEEECCCCCCCEECCCCCCCCCHHHCCCCEECCCCCCCCCC
INESDMVLMPDPTTFVLDPFTEETTALIRCDILEPGTMTGYDRDPRSIAKKAEAYLISTG
CCCCCEEEECCCCEEEECCCCCCCEEEEEEEECCCCCCCCCCCCHHHHHHHHHHEEEECC
IADTVLIGPEPEFFLFDDVRFGTDMSGCFVKIDAKEAAWNSGTSYEGGNTGHRPFTKGGY
CCCEEEECCCCCEEEEECEECCCCCCCEEEEEECHHHCCCCCCCCCCCCCCCCCCCCCCC
FPVAPVDSSQDLRSAMCLVLEEMGQVVEAHHHEVATAGQNEIATRFNTLTKKADEIQILK
EEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
YVVHNMAHAYGKTATFMPKPIVGDNGSGMHVHQSLAKDGVNLFAGDKYAGLSETALYYIG
HHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCEEEECCCCCCCHHHHHHHHH
GIIKHARALNAFTNPSTNSYKRLVPHFEAPVMLAYSARNRSASIRIPVVPSPKGRRIEAR
HHHHHHHHHHHCCCCCCCCHHHHCCCCCCCEEEEEECCCCCCEEEEEECCCCCCCEEEEE
FPDPHANPYLGFAALLMAGLDGIQNKIHPGEAMDKDLYDLPPEEAAEIPQVATSLENALE
CCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHCCCHHCCCCHHHHHHHHHHHHHHHHHH
NLQADHEFLTKGGVFSEDFIQSYIVLKTAEAERVARTTHPLEFEMYYSL
HHHHHHHHHHCCCCCCHHHHHHHHEEEECCHHHHHHHCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]