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The map label for this gene is pckA [H]

Identifier: 160873275

GI number: 160873275

Start: 176951

End: 178492

Strand: Reverse

Name: pckA [H]

Synonym: Sbal195_0149

Alternate gene names: 160873275

Gene position: 178492-176951 (Counterclockwise)

Preceding gene: 160873276

Following gene: 160873274

Centisome position: 3.34

GC content: 50.78

Gene sequence:

>1542_bases
ATGGCGGATGGATTAAACCGCGTTCACTTTAACCCCTCAACAGCACAACTTGTCGAATTTGCCCTGCTACGCGGCGAAGG
TGAATTGACAGCCAATGGCGCATTAGTCGCTAAAACAGGCGCTCGCAGTGGTCGCTCACCCGGCGATCGTTTTATCGTCA
GAGAACCCAGTTCAGAAGCAGAGATTGAATGGGGCCCAGTCAATCAAGCTTTCGATCCAGGTGCTTTTGAAGGTTTATGG
GCACGTGTTGAAGCTTACCTAGCCGATAAAGAACTGTTTGTCTCGGATCTCGAAGTCGGTGCCGATACCGAACATTACCA
ACCAGTGCGTGTGACCACGCAATACGCTTGGCACCAACTGTTCGCCCGCAATCTATTTATCATTCCTGAAGAATTTAATC
GTAAAGACAAACCAGTGTGGCAGATCATCAATGCCCCTGACTTTGTTTGCGATCCAGAACGTGATGGCACTAACTCAGAT
GCGACCGTGATTTTAAACTTCGCCGAACGCAAAGTGCTGCTCGCTGGCCTGAAATACGCTGGCGAAATGAAAAAGTCTAT
GTTCTCAGTGCAGAACTTCCTACTGCCAGCCCAAGGCGTGCTACCTATGCACTGCTCGGCCAACGTAGGTAAAGATGGCG
ACACTACGCTATTTTTCGGTTTATCTGGCACAGGTAAAACCACACTCTCTGCCGATCCAAAACGCTTCCTGATCGGTGAC
GATGAACACGGCTGGGCACCCGGCGGCGTATTCAATATCGAAGGCGGTTGCTACGCCAAATGTATCGATTTAAGCCAGAA
GAATGAACCTGTTATTTGGGATGCGATTCGTTTCGGTACTGTGCTCGAAAACGTGGTAATGGACGAACACCGCGTACCCA
ACTATAAAGACAGCAGCCTAACGGAAAACACCCGCGCAGCCTATCCGCTGGAGCACATCGCCCAACGTAAAGAAGACAAC
TGCGGCGCCGAGCCACATGCCGTTGTGTTCTTAACCTGCGACGTGTCTGGCGTATTACCACCGGTTTCTATCCTGACCAA
AGAACAAGCGGCATACCACTTCTTGTCTGGCTACACCGCTAAAGTGGGTTCGACTGAAATCGGCTCAACCTCAGCGATCC
AATCGACCTTCTCAACGTGCTTCGGAGCACCTTTCTTCCCACGCCCTGCTGGCGTGTACGCTGAACTGTTGATGAAGCGC
ATTGAGTCCTTCGGTAGCCAAGTGTACTTAGTCAACACTGGCTGGACTGGCGGTCCACACGGTGTGGGTAAACGTTTTGA
CATCCCCACCACTCGCGCCATCGTCGATGCGATTGTCAGCGGTGAATTAAAAGATGTTGAAACCGTCCACTTAGACACAT
TGAACCTCGCCGTTCCTGTGGCAGTAAGTGGTGTAGACAGCAATTTACTCAACCCAATCAACACTTGGGGCGATAAAGCG
CTATACGCCGAATATGCCCAGAAGCTGGCCGAAGCTTTCACTAAAAACTTCGCTAAGTATCAAGTGTCAGACGCTATCCG
CAACGCTGGTCCAAAGGCATAA

Upstream 100 bases:

>100_bases
GCCTCACTTTGTTACCTAGCAAAGCTTAGGTGATAAATAAAATAAAATCGGCAGACGATCGATAACAAAATAACCTTACA
AATCAAATGGAGACCTCACT

Downstream 100 bases:

>100_bases
ACGCTTAAGTATTAAGCCATAGCCTTACTTGTAAAAAAGCGCCGACCCAGCAATGAGTCGGCGCTTTTTATTGTCGTGCC
AAAGGCAAATACTTAGTCTT

Product: phosphoenolpyruvate carboxykinase

Products: NA

Alternate protein names: PEP carboxykinase; PEPCK; Phosphoenolpyruvate carboxylase [H]

Number of amino acids: Translated: 513; Mature: 512

Protein sequence:

>513_residues
MADGLNRVHFNPSTAQLVEFALLRGEGELTANGALVAKTGARSGRSPGDRFIVREPSSEAEIEWGPVNQAFDPGAFEGLW
ARVEAYLADKELFVSDLEVGADTEHYQPVRVTTQYAWHQLFARNLFIIPEEFNRKDKPVWQIINAPDFVCDPERDGTNSD
ATVILNFAERKVLLAGLKYAGEMKKSMFSVQNFLLPAQGVLPMHCSANVGKDGDTTLFFGLSGTGKTTLSADPKRFLIGD
DEHGWAPGGVFNIEGGCYAKCIDLSQKNEPVIWDAIRFGTVLENVVMDEHRVPNYKDSSLTENTRAAYPLEHIAQRKEDN
CGAEPHAVVFLTCDVSGVLPPVSILTKEQAAYHFLSGYTAKVGSTEIGSTSAIQSTFSTCFGAPFFPRPAGVYAELLMKR
IESFGSQVYLVNTGWTGGPHGVGKRFDIPTTRAIVDAIVSGELKDVETVHLDTLNLAVPVAVSGVDSNLLNPINTWGDKA
LYAEYAQKLAEAFTKNFAKYQVSDAIRNAGPKA

Sequences:

>Translated_513_residues
MADGLNRVHFNPSTAQLVEFALLRGEGELTANGALVAKTGARSGRSPGDRFIVREPSSEAEIEWGPVNQAFDPGAFEGLW
ARVEAYLADKELFVSDLEVGADTEHYQPVRVTTQYAWHQLFARNLFIIPEEFNRKDKPVWQIINAPDFVCDPERDGTNSD
ATVILNFAERKVLLAGLKYAGEMKKSMFSVQNFLLPAQGVLPMHCSANVGKDGDTTLFFGLSGTGKTTLSADPKRFLIGD
DEHGWAPGGVFNIEGGCYAKCIDLSQKNEPVIWDAIRFGTVLENVVMDEHRVPNYKDSSLTENTRAAYPLEHIAQRKEDN
CGAEPHAVVFLTCDVSGVLPPVSILTKEQAAYHFLSGYTAKVGSTEIGSTSAIQSTFSTCFGAPFFPRPAGVYAELLMKR
IESFGSQVYLVNTGWTGGPHGVGKRFDIPTTRAIVDAIVSGELKDVETVHLDTLNLAVPVAVSGVDSNLLNPINTWGDKA
LYAEYAQKLAEAFTKNFAKYQVSDAIRNAGPKA
>Mature_512_residues
ADGLNRVHFNPSTAQLVEFALLRGEGELTANGALVAKTGARSGRSPGDRFIVREPSSEAEIEWGPVNQAFDPGAFEGLWA
RVEAYLADKELFVSDLEVGADTEHYQPVRVTTQYAWHQLFARNLFIIPEEFNRKDKPVWQIINAPDFVCDPERDGTNSDA
TVILNFAERKVLLAGLKYAGEMKKSMFSVQNFLLPAQGVLPMHCSANVGKDGDTTLFFGLSGTGKTTLSADPKRFLIGDD
EHGWAPGGVFNIEGGCYAKCIDLSQKNEPVIWDAIRFGTVLENVVMDEHRVPNYKDSSLTENTRAAYPLEHIAQRKEDNC
GAEPHAVVFLTCDVSGVLPPVSILTKEQAAYHFLSGYTAKVGSTEIGSTSAIQSTFSTCFGAPFFPRPAGVYAELLMKRI
ESFGSQVYLVNTGWTGGPHGVGKRFDIPTTRAIVDAIVSGELKDVETVHLDTLNLAVPVAVSGVDSNLLNPINTWGDKAL
YAEYAQKLAEAFTKNFAKYQVSDAIRNAGPKA

Specific function: Rate-limiting gluconeogenic enzyme. [C]

COG id: COG1866

COG function: function code C; Phosphoenolpyruvate carboxykinase (ATP)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoenolpyruvate carboxykinase [ATP] family [H]

Homologues:

Organism=Escherichia coli, GI1789807, Length=532, Percent_Identity=45.4887218045113, Blast_Score=412, Evalue=1e-116,
Organism=Saccharomyces cerevisiae, GI6322950, Length=524, Percent_Identity=44.4656488549618, Blast_Score=414, Evalue=1e-116,

Paralogues:

None

Copy number: 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001272
- InterPro:   IPR013035
- InterPro:   IPR008210
- InterPro:   IPR015994 [H]

Pfam domain/function: PF01293 PEPCK_ATP [H]

EC number: =4.1.1.49 [H]

Molecular weight: Translated: 55951; Mature: 55819

Theoretical pI: Translated: 5.05; Mature: 5.05

Prosite motif: PS00532 PEPCK_ATP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADGLNRVHFNPSTAQLVEFALLRGEGELTANGALVAKTGARSGRSPGDRFIVREPSSEA
CCCCCCEEEECCCHHHHHHHHHHCCCCCEEECCEEEEECCCCCCCCCCCEEEEECCCCCC
EIEWGPVNQAFDPGAFEGLWARVEAYLADKELFVSDLEVGADTEHYQPVRVTTQYAWHQL
EEEECCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCEEEHHHHHHHHH
FARNLFIIPEEFNRKDKPVWQIINAPDFVCDPERDGTNSDATVILNFAERKVLLAGLKYA
HHCCEEEECHHCCCCCCCCEEEECCCCCEECCCCCCCCCCCEEEEEEHHHEHHHHHHHHH
GEMKKSMFSVQNFLLPAQGVLPMHCSANVGKDGDTTLFFGLSGTGKTTLSADPKRFLIGD
HHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCEEEEEECCCCCEEECCCCCEEEECC
DEHGWAPGGVFNIEGGCYAKCIDLSQKNEPVIWDAIRFGTVLENVVMDEHRVPNYKDSSL
CCCCCCCCCEEEECCCCEEEEEECCCCCCCEEEHHHHHHHHHHHHHHHHCCCCCCCCCCC
TENTRAAYPLEHIAQRKEDNCGAEPHAVVFLTCDVSGVLPPVSILTKEQAAYHFLSGYTA
CCCCCCCCCHHHHHHHCCCCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHCCCEE
KVGSTEIGSTSAIQSTFSTCFGAPFFPRPAGVYAELLMKRIESFGSQVYLVNTGWTGGPH
ECCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCEEEEEECCCCCCCC
GVGKRFDIPTTRAIVDAIVSGELKDVETVHLDTLNLAVPVAVSGVDSNLLNPINTWGDKA
CCCCCCCCCHHHHHHHHHHCCCCCCCEEEEEEEEEEEEEEEEECCCCHHCCCHHHCCCHH
LYAEYAQKLAEAFTKNFAKYQVSDAIRNAGPKA
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
ADGLNRVHFNPSTAQLVEFALLRGEGELTANGALVAKTGARSGRSPGDRFIVREPSSEA
CCCCCEEEECCCHHHHHHHHHHCCCCCEEECCEEEEECCCCCCCCCCCEEEEECCCCCC
EIEWGPVNQAFDPGAFEGLWARVEAYLADKELFVSDLEVGADTEHYQPVRVTTQYAWHQL
EEEECCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCEEEHHHHHHHHH
FARNLFIIPEEFNRKDKPVWQIINAPDFVCDPERDGTNSDATVILNFAERKVLLAGLKYA
HHCCEEEECHHCCCCCCCCEEEECCCCCEECCCCCCCCCCCEEEEEEHHHEHHHHHHHHH
GEMKKSMFSVQNFLLPAQGVLPMHCSANVGKDGDTTLFFGLSGTGKTTLSADPKRFLIGD
HHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCEEEEEECCCCCEEECCCCCEEEECC
DEHGWAPGGVFNIEGGCYAKCIDLSQKNEPVIWDAIRFGTVLENVVMDEHRVPNYKDSSL
CCCCCCCCCEEEECCCCEEEEEECCCCCCCEEEHHHHHHHHHHHHHHHHCCCCCCCCCCC
TENTRAAYPLEHIAQRKEDNCGAEPHAVVFLTCDVSGVLPPVSILTKEQAAYHFLSGYTA
CCCCCCCCCHHHHHHHCCCCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHCCCEE
KVGSTEIGSTSAIQSTFSTCFGAPFFPRPAGVYAELLMKRIESFGSQVYLVNTGWTGGPH
ECCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCEEEEEECCCCCCCC
GVGKRFDIPTTRAIVDAIVSGELKDVETVHLDTLNLAVPVAVSGVDSNLLNPINTWGDKA
CCCCCCCCCHHHHHHHHHHCCCCCCCEEEEEEEEEEEEEEEEECCCCHHCCCHHHCCCHH
LYAEYAQKLAEAFTKNFAKYQVSDAIRNAGPKA
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA