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The map label for this gene is dosC [H]

Identifier: 160873144

GI number: 160873144

Start: 20608

End: 21771

Strand: Reverse

Name: dosC [H]

Synonym: Sbal195_0018

Alternate gene names: 160873144

Gene position: 21771-20608 (Counterclockwise)

Preceding gene: 160873145

Following gene: 160873143

Centisome position: 0.41

GC content: 44.67

Gene sequence:

>1164_bases
ATGCTACAAACGGAACAGACCCTTTTCGAACAAATGCGGATCACTGAGCTGGAGATCGAGTCTCGTAAGGCGCTCTTCTC
TTTTACTGGGGCGGACGTAAAGGCCCTGCAATCCTTTAAGCCCATCATTGAAGAAAACATAGATAAAATCGTCGATGACT
TTTATGGCTTACAAACCAGCGTTTCAGAAATTGCGCTGCTGATCGGAGACTCAGACACCCTTGCCCGCTTACGTACCGCA
CAGAGAAGATATGTGCTCGATTTATTTAATGGTGTTTATGATCTTGAGTACGTAAACAATCGCCTGCGGATTGGGTTGGT
CCATAAACGCATTGGTGTTGAGCCTAAGCTTTACCTATCTGCGGTACACACGCTAAAAGAGCTTATCTACGCCGTGATCA
ACTGTTCAGTGCAAGATACACATCAAGGCGAATGTATCCGTGTCGCGATCGATAAACTGGTGCTGTTTGATGTCACGTTA
GTATTCGATACTTATATTCGCAGCTTAGTATCCGAAATTGAGAACGCTAAGGATAAAGCCGAAAAATATGCCCAAAGCAT
GGAAAGCAAAGTCAAAGAGCGGACACAGCAGTTAGAAGAACTGTCGCAAACCGATCCCTTAACAGGCCTGCTCAACGTTA
GGCATTTACAAGAAACGGCAACGCGCATACTCAGGGCCGCCCAAAGGCGTGCAGAACCCGTCAGCGTGATCTACTTAGAC
GTAGATGATTTTAAGTCATTCAACGACACCCAAGGCCATAGATCTGGGGATGAAGTACTGCGCGCTGTAGCAAAAGCGAT
TAAAGAAATCACTCGGGCCGAGGACCATTGCTTTCGATATGGTGGAGATGAGTTCTGTATCATCATGCCTAACTGCCGTG
AAGAACAAGCTCGGGATAATTTCATCACTCGATTCAATCAAAAAATAAAACTCAGTTTAAACAATATTTCCTTAAGCTTT
GGCATTATTGATACTGGACCCTATGAGTACGAAGAAGCCAATAGCTTAATTCATAAAGCCGATCAGCGAATGTATAGCTA
CAAACGTGCGTCCAAACAGCAGCAACAAACTATGGCGGCAGCAAATGATTTAGCACCTGAGAATAGTACCGCTGATGTCC
ATTCAATCTCTCAATCTCAAAGCGTTGATAAAGCCGTCGAGTAA

Upstream 100 bases:

>100_bases
TCACTGGCTATTGTGGACGACAACAGGCAAGCAATCATCAGCCAAATCTAGGCAGTGTAGTGCAAGGATGCAATTCGTTG
TTTTACAAGAGGAATGCTTG

Downstream 100 bases:

>100_bases
TTTTTAAACCCATTCGTCATCATGTTCCACGTGGAACATTAAATATCTAACATTCGTATTGATAGAACACATCAATAAAA
CTAGATCACAAATTGAAACC

Product: diguanylate cyclase

Products: NA

Alternate protein names: DGC [H]

Number of amino acids: Translated: 387; Mature: 387

Protein sequence:

>387_residues
MLQTEQTLFEQMRITELEIESRKALFSFTGADVKALQSFKPIIEENIDKIVDDFYGLQTSVSEIALLIGDSDTLARLRTA
QRRYVLDLFNGVYDLEYVNNRLRIGLVHKRIGVEPKLYLSAVHTLKELIYAVINCSVQDTHQGECIRVAIDKLVLFDVTL
VFDTYIRSLVSEIENAKDKAEKYAQSMESKVKERTQQLEELSQTDPLTGLLNVRHLQETATRILRAAQRRAEPVSVIYLD
VDDFKSFNDTQGHRSGDEVLRAVAKAIKEITRAEDHCFRYGGDEFCIIMPNCREEQARDNFITRFNQKIKLSLNNISLSF
GIIDTGPYEYEEANSLIHKADQRMYSYKRASKQQQQTMAAANDLAPENSTADVHSISQSQSVDKAVE

Sequences:

>Translated_387_residues
MLQTEQTLFEQMRITELEIESRKALFSFTGADVKALQSFKPIIEENIDKIVDDFYGLQTSVSEIALLIGDSDTLARLRTA
QRRYVLDLFNGVYDLEYVNNRLRIGLVHKRIGVEPKLYLSAVHTLKELIYAVINCSVQDTHQGECIRVAIDKLVLFDVTL
VFDTYIRSLVSEIENAKDKAEKYAQSMESKVKERTQQLEELSQTDPLTGLLNVRHLQETATRILRAAQRRAEPVSVIYLD
VDDFKSFNDTQGHRSGDEVLRAVAKAIKEITRAEDHCFRYGGDEFCIIMPNCREEQARDNFITRFNQKIKLSLNNISLSF
GIIDTGPYEYEEANSLIHKADQRMYSYKRASKQQQQTMAAANDLAPENSTADVHSISQSQSVDKAVE
>Mature_387_residues
MLQTEQTLFEQMRITELEIESRKALFSFTGADVKALQSFKPIIEENIDKIVDDFYGLQTSVSEIALLIGDSDTLARLRTA
QRRYVLDLFNGVYDLEYVNNRLRIGLVHKRIGVEPKLYLSAVHTLKELIYAVINCSVQDTHQGECIRVAIDKLVLFDVTL
VFDTYIRSLVSEIENAKDKAEKYAQSMESKVKERTQQLEELSQTDPLTGLLNVRHLQETATRILRAAQRRAEPVSVIYLD
VDDFKSFNDTQGHRSGDEVLRAVAKAIKEITRAEDHCFRYGGDEFCIIMPNCREEQARDNFITRFNQKIKLSLNNISLSF
GIIDTGPYEYEEANSLIHKADQRMYSYKRASKQQQQTMAAANDLAPENSTADVHSISQSQSVDKAVE

Specific function: Overexpression leads to an increased level of c-di-GMP, which leads to changes in the cell surface, to abnormal cell division, increased biofilm formation and decreased swimming (the latter 2 in strain W3110). In a strain able to produce cellulose (strain

COG id: COG2199

COG function: function code T; FOG: GGDEF domain

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GGDEF domain [H]

Homologues:

Organism=Escherichia coli, GI145693134, Length=177, Percent_Identity=37.2881355932203, Blast_Score=98, Evalue=9e-22,
Organism=Escherichia coli, GI87082007, Length=172, Percent_Identity=34.8837209302326, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI1786584, Length=176, Percent_Identity=27.8409090909091, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI1787541, Length=171, Percent_Identity=30.9941520467836, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI87081977, Length=185, Percent_Identity=30.8108108108108, Blast_Score=75, Evalue=7e-15,
Organism=Escherichia coli, GI87081881, Length=131, Percent_Identity=34.3511450381679, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1788956, Length=172, Percent_Identity=29.0697674418605, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1787816, Length=166, Percent_Identity=33.7349397590361, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI1788381, Length=111, Percent_Identity=33.3333333333333, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI87081974, Length=151, Percent_Identity=32.4503311258278, Blast_Score=72, Evalue=9e-14,
Organism=Escherichia coli, GI1787262, Length=100, Percent_Identity=32, Blast_Score=68, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160
- InterPro:   IPR012292
- InterPro:   IPR009050 [H]

Pfam domain/function: PF00990 GGDEF [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 44164; Mature: 44164

Theoretical pI: Translated: 5.23; Mature: 5.23

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLQTEQTLFEQMRITELEIESRKALFSFTGADVKALQSFKPIIEENIDKIVDDFYGLQTS
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VSEIALLIGDSDTLARLRTAQRRYVLDLFNGVYDLEYVNNRLRIGLVHKRIGVEPKLYLS
HHHEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHCCCHHHHHH
AVHTLKELIYAVINCSVQDTHQGECIRVAIDKLVLFDVTLVFDTYIRSLVSEIENAKDKA
HHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EKYAQSMESKVKERTQQLEELSQTDPLTGLLNVRHLQETATRILRAAQRRAEPVSVIYLD
HHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEE
VDDFKSFNDTQGHRSGDEVLRAVAKAIKEITRAEDHCFRYGGDEFCIIMPNCREEQARDN
HHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHH
FITRFNQKIKLSLNNISLSFGIIDTGPYEYEEANSLIHKADQRMYSYKRASKQQQQTMAA
HHHHCCCEEEEEEEEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ANDLAPENSTADVHSISQSQSVDKAVE
HHHCCCCCCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MLQTEQTLFEQMRITELEIESRKALFSFTGADVKALQSFKPIIEENIDKIVDDFYGLQTS
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VSEIALLIGDSDTLARLRTAQRRYVLDLFNGVYDLEYVNNRLRIGLVHKRIGVEPKLYLS
HHHEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHCCCHHHHHH
AVHTLKELIYAVINCSVQDTHQGECIRVAIDKLVLFDVTLVFDTYIRSLVSEIENAKDKA
HHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EKYAQSMESKVKERTQQLEELSQTDPLTGLLNVRHLQETATRILRAAQRRAEPVSVIYLD
HHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEE
VDDFKSFNDTQGHRSGDEVLRAVAKAIKEITRAEDHCFRYGGDEFCIIMPNCREEQARDN
HHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHH
FITRFNQKIKLSLNNISLSFGIIDTGPYEYEEANSLIHKADQRMYSYKRASKQQQQTMAA
HHHHCCCEEEEEEEEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ANDLAPENSTADVHSISQSQSVDKAVE
HHHCCCCCCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]