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The map label for this gene is ndhF [H]

Identifier: 159901499

GI number: 159901499

Start: 6323038

End: 6325248

Strand: Direct

Name: ndhF [H]

Synonym: Haur_4988

Alternate gene names: 159901499

Gene position: 6323038-6325248 (Clockwise)

Preceding gene: 159901498

Following gene: 159901500

Centisome position: 99.63

GC content: 51.88

Gene sequence:

>2211_bases
ATGCCGTTTTTTGATCTTGCGTGGTTGATTCCGCTGCTGCCATTGGCAGCATTTGTGTTGATCACCCTCGTGCCACCGAT
TGGGCGCTCGCGCAAAGCGTCGTATACCACGGCGTTAGTCTTGCTTGGCCTGGCTACGGTGATTGCCTGGGGTGTTTTAG
CACAATCGATCAGCGAAGGATTCCCTAATGCACCATTGGTTGCCGCAGCTAGCCAAGAATCGGCCAGCGAGGCTACCGCT
GAAGGTGGCCATGCGGAAACCGCAATTCCAACATTTGGGAATTTCTATGCTAAATCGTTTGCATGGGCACCAGATGGTTC
AGGGTTTTTTAAATTTGGCTATCGCTTAGATGGTGCTACGGTGCTGATGTTGGCGATGGTCACGCTGGCTGCATGGTGTA
TTCACCTCTTCTCAGTGGGCTATATGGCTCACGATGAATTCCCCACAGGCCACCAACGCCAATCGCGCTTCTTCTCCTAT
ATTGCGTTATTTACCGCTTCGATGTTGGGAATGACGCTGGCAGATAATTTATTGCTGTTCTTTATCTGCTGGGAGTTGAT
GGGTCTGTGTTCGTTCTTGTTGATTGGTTTTTGGTTCTTCAAGCCCTCGGCTCGCGAAGCTGCCAAAAAAGCCTTTATCA
CCACCCGGATTGGTGATGTGGGCATGATGTTGGGGATGATGTACATCTACAACAAGGCTGGCTCGTTGACCTTCGGCCAT
GAACCAGGCCAAATTTACAACGGCGAATTTTTGGAAAGCATCAAAACTGCTACGAGCTTGATTCCTGGAATTAGCGCTGC
TTCTTTGATGGCAGGCTTAGTATTTCTTGGCACGGTTGGCAAATCGGCGCAGTTTCCGCTGCACGTTTGGTTGCCCGATG
CGATGGAAGGCCCAACGCCTGTTTCAGCGTTGATTCACGCTGCGACCATGGTTGCGGCTGGGGTGTTCTTGGTCATTCGC
ACTTTCCCAATCTTTGCGGTCAGCGAAGTTTTGCCAGTAGTCGCCTTTATTGGCGCATTCACGGCCTTGTTCGCAGCCCT
GATTGCGGTGGCGCAATATGACATCAAGCGCATTCTGGCCTTCTCGACCTTATCGCAGCTTGGTTTCATGGTGGCAGCAC
TAGGGATTGGCGCTTGGGTTGCAGCGCTGTTCCACTTGCTGACCCACGCGTTCTTTAAAGCACTCTTGTTCCTTGGTTCT
GGCTCGGTGATCCACGGGATGGAAGCAACCGTTGGCCATGATCCTGATAAAGCCCAAGATATCCGCAACATGGGTACCTT
GCGCAAATTCATGCCAATCACCTTCTTGACCTATATGGCTGGTTTCTTCGCCTTGATTGGGATTCCACCGTTTGCTGGGT
TCTGGTCGAAGGACGAAATTATCCTTGATGCCTTCCTGCATCACCATTATGTGGTGTATGGTGTGCTGACCGCCGCCGCT
TTCTTGACGGCGTTCTATATGACCCGCCAAATTGTGGTGGTGTTCTTTGGCAAGTTCCGTGGCTACGAACCACGGAAGCT
GGCTCACGCTGCGGCTCATGGTCACGATGATCACGGTCATGGCGACCACGACGACCATCACCATCATCACGAAGCCCATG
ATCCCCACGAATCGCCGCGCTCGATGACGATTCCGTTGATCATTTTGAGCTTGTTTGCGGTGTTTGCTGGGTTTGTCAAC
GCTGGCTTCTTGGGCATTCACTGGTTTGCTGACTATGTGAACCCAGGCGGACTTATTCCTAAACCTGACTTCATGGTGGC
GGGGATTGCCACGGCGGTAGCTTTGGTTGGTGCTGGTTTAGGCTATTTGACCTATCGCAACGCCTTCAAAAATGCCAATG
ATCGTGACCCCTTGGCCGGAGCTTTGGGGCCAATCTGGACGCTGTTGGAAAATCGCTTCTACATCGACCAGATTTACGAC
AAGACCTTTATTGCCCTGACCTATGGCGCAGGCCAAGTAATGAACTGGATCGACCGCCATGTGGTTGATCGGGTGGTCAA
CTTGACAGGCTTGCTGACCTTGTTTATTGGCCGCATCAACTTCATCATCGACGACTTCTCGTTGAATACCGTCACCGACG
ATATTGGCGAAGGCACGATTATGGTGGGCGATGGGGTGCGTCAATCGGCCACGGGCAAGATTCAAGACTACGGTGCCTAC
ATCTTTGGTGGCGTGGTGCTGTTGGCCTTGATTTATATGTATGCCTTCTAG

Upstream 100 bases:

>100_bases
TAGATACACTTAAAGGATAGTGACGTTTTGCCAAGGCCGGCGAGAGTGCCCAGCACTTTGACCACCCTTTGTAATTCGTC
TCGAAGTAGGGGACTGATCA

Downstream 100 bases:

>100_bases
GAGCGTGAGCGAATGCTGAACGAATTTATCAAATTCAGTGATTGGAGCATCACCACGCTAATTGCCCTTTCGCCATTGGT
GGGGATGTTGCTGGCCTTGG

Product: proton-translocating NADH-quinone oxidoreductase subunit L

Products: NAD+; ubiquinol

Alternate protein names: NAD(P)H dehydrogenase I, chain 5; NDH-1, chain 5 [H]

Number of amino acids: Translated: 736; Mature: 735

Protein sequence:

>736_residues
MPFFDLAWLIPLLPLAAFVLITLVPPIGRSRKASYTTALVLLGLATVIAWGVLAQSISEGFPNAPLVAAASQESASEATA
EGGHAETAIPTFGNFYAKSFAWAPDGSGFFKFGYRLDGATVLMLAMVTLAAWCIHLFSVGYMAHDEFPTGHQRQSRFFSY
IALFTASMLGMTLADNLLLFFICWELMGLCSFLLIGFWFFKPSAREAAKKAFITTRIGDVGMMLGMMYIYNKAGSLTFGH
EPGQIYNGEFLESIKTATSLIPGISAASLMAGLVFLGTVGKSAQFPLHVWLPDAMEGPTPVSALIHAATMVAAGVFLVIR
TFPIFAVSEVLPVVAFIGAFTALFAALIAVAQYDIKRILAFSTLSQLGFMVAALGIGAWVAALFHLLTHAFFKALLFLGS
GSVIHGMEATVGHDPDKAQDIRNMGTLRKFMPITFLTYMAGFFALIGIPPFAGFWSKDEIILDAFLHHHYVVYGVLTAAA
FLTAFYMTRQIVVVFFGKFRGYEPRKLAHAAAHGHDDHGHGDHDDHHHHHEAHDPHESPRSMTIPLIILSLFAVFAGFVN
AGFLGIHWFADYVNPGGLIPKPDFMVAGIATAVALVGAGLGYLTYRNAFKNANDRDPLAGALGPIWTLLENRFYIDQIYD
KTFIALTYGAGQVMNWIDRHVVDRVVNLTGLLTLFIGRINFIIDDFSLNTVTDDIGEGTIMVGDGVRQSATGKIQDYGAY
IFGGVVLLALIYMYAF

Sequences:

>Translated_736_residues
MPFFDLAWLIPLLPLAAFVLITLVPPIGRSRKASYTTALVLLGLATVIAWGVLAQSISEGFPNAPLVAAASQESASEATA
EGGHAETAIPTFGNFYAKSFAWAPDGSGFFKFGYRLDGATVLMLAMVTLAAWCIHLFSVGYMAHDEFPTGHQRQSRFFSY
IALFTASMLGMTLADNLLLFFICWELMGLCSFLLIGFWFFKPSAREAAKKAFITTRIGDVGMMLGMMYIYNKAGSLTFGH
EPGQIYNGEFLESIKTATSLIPGISAASLMAGLVFLGTVGKSAQFPLHVWLPDAMEGPTPVSALIHAATMVAAGVFLVIR
TFPIFAVSEVLPVVAFIGAFTALFAALIAVAQYDIKRILAFSTLSQLGFMVAALGIGAWVAALFHLLTHAFFKALLFLGS
GSVIHGMEATVGHDPDKAQDIRNMGTLRKFMPITFLTYMAGFFALIGIPPFAGFWSKDEIILDAFLHHHYVVYGVLTAAA
FLTAFYMTRQIVVVFFGKFRGYEPRKLAHAAAHGHDDHGHGDHDDHHHHHEAHDPHESPRSMTIPLIILSLFAVFAGFVN
AGFLGIHWFADYVNPGGLIPKPDFMVAGIATAVALVGAGLGYLTYRNAFKNANDRDPLAGALGPIWTLLENRFYIDQIYD
KTFIALTYGAGQVMNWIDRHVVDRVVNLTGLLTLFIGRINFIIDDFSLNTVTDDIGEGTIMVGDGVRQSATGKIQDYGAY
IFGGVVLLALIYMYAF
>Mature_735_residues
PFFDLAWLIPLLPLAAFVLITLVPPIGRSRKASYTTALVLLGLATVIAWGVLAQSISEGFPNAPLVAAASQESASEATAE
GGHAETAIPTFGNFYAKSFAWAPDGSGFFKFGYRLDGATVLMLAMVTLAAWCIHLFSVGYMAHDEFPTGHQRQSRFFSYI
ALFTASMLGMTLADNLLLFFICWELMGLCSFLLIGFWFFKPSAREAAKKAFITTRIGDVGMMLGMMYIYNKAGSLTFGHE
PGQIYNGEFLESIKTATSLIPGISAASLMAGLVFLGTVGKSAQFPLHVWLPDAMEGPTPVSALIHAATMVAAGVFLVIRT
FPIFAVSEVLPVVAFIGAFTALFAALIAVAQYDIKRILAFSTLSQLGFMVAALGIGAWVAALFHLLTHAFFKALLFLGSG
SVIHGMEATVGHDPDKAQDIRNMGTLRKFMPITFLTYMAGFFALIGIPPFAGFWSKDEIILDAFLHHHYVVYGVLTAAAF
LTAFYMTRQIVVVFFGKFRGYEPRKLAHAAAHGHDDHGHGDHDDHHHHHEAHDPHESPRSMTIPLIILSLFAVFAGFVNA
GFLGIHWFADYVNPGGLIPKPDFMVAGIATAVALVGAGLGYLTYRNAFKNANDRDPLAGALGPIWTLLENRFYIDQIYDK
TFIALTYGAGQVMNWIDRHVVDRVVNLTGLLTLFIGRINFIIDDFSLNTVTDDIGEGTIMVGDGVRQSATGKIQDYGAYI
FGGVVLLALIYMYAF

Specific function: NDH-1 shuttles electrons from NAD(P)H, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be plastoquinone. Couples the redox reaction to proton tran

COG id: COG1009

COG function: function code CP; NADH:ubiquinone oxidoreductase subunit 5 (chain L)/Multisubunit Na+/H+ antiporter, MnhA subunit

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 5 family [H]

Homologues:

Organism=Homo sapiens, GI251831117, Length=401, Percent_Identity=38.9027431421446, Blast_Score=244, Evalue=2e-64,
Organism=Escherichia coli, GI1788614, Length=596, Percent_Identity=36.5771812080537, Blast_Score=306, Evalue=3e-84,
Organism=Escherichia coli, GI1788829, Length=356, Percent_Identity=29.7752808988764, Blast_Score=142, Evalue=9e-35,
Organism=Escherichia coli, GI1788831, Length=382, Percent_Identity=30.1047120418848, Blast_Score=129, Evalue=7e-31,
Organism=Escherichia coli, GI1788827, Length=369, Percent_Identity=31.9783197831978, Blast_Score=128, Evalue=2e-30,
Organism=Escherichia coli, GI1788613, Length=314, Percent_Identity=28.9808917197452, Blast_Score=89, Evalue=1e-18,
Organism=Escherichia coli, GI145693160, Length=390, Percent_Identity=26.1538461538462, Blast_Score=86, Evalue=1e-17,
Organism=Escherichia coli, GI2367154, Length=321, Percent_Identity=23.98753894081, Blast_Score=77, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001750
- InterPro:   IPR001516
- InterPro:   IPR002128
- InterPro:   IPR003945
- InterPro:   IPR018393 [H]

Pfam domain/function: PF00361 Oxidored_q1; PF01010 Oxidored_q1_C; PF00662 Oxidored_q1_N [H]

EC number: 1.6.5.3

Molecular weight: Translated: 80080; Mature: 79949

Theoretical pI: Translated: 6.72; Mature: 6.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPFFDLAWLIPLLPLAAFVLITLVPPIGRSRKASYTTALVLLGLATVIAWGVLAQSISEG
CCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC
FPNAPLVAAASQESASEATAEGGHAETAIPTFGNFYAKSFAWAPDGSGFFKFGYRLDGAT
CCCCCEEEECCCHHHHHHHCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCEEECEEECHHH
VLMLAMVTLAAWCIHLFSVGYMAHDEFPTGHQRQSRFFSYIALFTASMLGMTLADNLLLF
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FICWELMGLCSFLLIGFWFFKPSAREAAKKAFITTRIGDVGMMLGMMYIYNKAGSLTFGH
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECC
EPGQIYNGEFLESIKTATSLIPGISAASLMAGLVFLGTVGKSAQFPLHVWLPDAMEGPTP
CCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCCH
VSALIHAATMVAAGVFLVIRTFPIFAVSEVLPVVAFIGAFTALFAALIAVAQYDIKRILA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FSTLSQLGFMVAALGIGAWVAALFHLLTHAFFKALLFLGSGSVIHGMEATVGHDPDKAQD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCHHCCCCCHHHHH
IRNMGTLRKFMPITFLTYMAGFFALIGIPPFAGFWSKDEIILDAFLHHHYVVYGVLTAAA
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
FLTAFYMTRQIVVVFFGKFRGYEPRKLAHAAAHGHDDHGHGDHDDHHHHHEAHDPHESPR
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCC
SMTIPLIILSLFAVFAGFVNAGFLGIHWFADYVNPGGLIPKPDFMVAGIATAVALVGAGL
CCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCH
GYLTYRNAFKNANDRDPLAGALGPIWTLLENRFYIDQIYDKTFIALTYGAGQVMNWIDRH
HHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHCCEEEEEECCHHHHHHHHHHH
VVDRVVNLTGLLTLFIGRINFIIDDFSLNTVTDDIGEGTIMVGDGVRQSATGKIQDYGAY
HHHHHHHHHHHHHHHHHHHHHHEECCCCCHHHHCCCCCEEEECCCCCCCCCCCHHHHHHH
IFGGVVLLALIYMYAF
HHHHHHHHHHHHHHCC
>Mature Secondary Structure 
PFFDLAWLIPLLPLAAFVLITLVPPIGRSRKASYTTALVLLGLATVIAWGVLAQSISEG
CCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC
FPNAPLVAAASQESASEATAEGGHAETAIPTFGNFYAKSFAWAPDGSGFFKFGYRLDGAT
CCCCCEEEECCCHHHHHHHCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCEEECEEECHHH
VLMLAMVTLAAWCIHLFSVGYMAHDEFPTGHQRQSRFFSYIALFTASMLGMTLADNLLLF
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FICWELMGLCSFLLIGFWFFKPSAREAAKKAFITTRIGDVGMMLGMMYIYNKAGSLTFGH
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECC
EPGQIYNGEFLESIKTATSLIPGISAASLMAGLVFLGTVGKSAQFPLHVWLPDAMEGPTP
CCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCCH
VSALIHAATMVAAGVFLVIRTFPIFAVSEVLPVVAFIGAFTALFAALIAVAQYDIKRILA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FSTLSQLGFMVAALGIGAWVAALFHLLTHAFFKALLFLGSGSVIHGMEATVGHDPDKAQD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCHHCCCCCHHHHH
IRNMGTLRKFMPITFLTYMAGFFALIGIPPFAGFWSKDEIILDAFLHHHYVVYGVLTAAA
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
FLTAFYMTRQIVVVFFGKFRGYEPRKLAHAAAHGHDDHGHGDHDDHHHHHEAHDPHESPR
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCC
SMTIPLIILSLFAVFAGFVNAGFLGIHWFADYVNPGGLIPKPDFMVAGIATAVALVGAGL
CCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCH
GYLTYRNAFKNANDRDPLAGALGPIWTLLENRFYIDQIYDKTFIALTYGAGQVMNWIDRH
HHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHCCEEEEEECCHHHHHHHHHHH
VVDRVVNLTGLLTLFIGRINFIIDDFSLNTVTDDIGEGTIMVGDGVRQSATGKIQDYGAY
HHHHHHHHHHHHHHHHHHHHHHEECCCCCHHHHCCCCCEEEECCCCCCCCCCCHHHHHHH
IFGGVVLLALIYMYAF
HHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADH; H+; ubiquinone

Specific reaction: NADH + H+ + ubiquinone = NAD+ + ubiquinol

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]