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The map label for this gene is nuoI2 [H]

Identifier: 159901495

GI number: 159901495

Start: 6319670

End: 6320662

Strand: Direct

Name: nuoI2 [H]

Synonym: Haur_4984

Alternate gene names: 159901495

Gene position: 6319670-6320662 (Clockwise)

Preceding gene: 159901494

Following gene: 159901496

Centisome position: 99.58

GC content: 52.97

Gene sequence:

>993_bases
ATGACGTTGAGCCACTTAGCAACTGAAGACGTGTTCGTTGTTGAACGCAATGACGCGGATTTTCGGGCTGGTCAGGGGAT
GACCAAGGGCTTGGGAATTGTGTTTCGCAAATTCCGCAAGGCTTTGTTTGGCAAACCCAACAGCGACCGCGCCGATACCG
AAGGCCAATTCAATGTGCAATATCCCGAAGAGCGGCTGGTGCTGCCTGAGGCTTATCGCAATATGCCAATTTTGCTCTAC
GATGATGCAACTGGCCATGAATTGTGTACTTCATGTTTCCAATGTGAGCGGATCTGCCCGCCGCAGGTGATTCACATTAC
CCAAGCGAAAGATCCCGCAACTGGTAAGCCAGTTCCAGCCGCTGCCAACTTTGTGTTGGAATACGACACCTGTATGTCGT
GTGGCTACTGTGCCGAAGTTTGTCCATTTGATGCAATCAAAATGGACCATGATTTTGAATTATCAACGGGCAACCACCCA
GAGCTTGATGTGAACTACGACCGTTTGTTGCGCCCAATCTCGTATTACGAAGCGATTGCGCCAACCATGTGGTCGGATGT
CAAAGAACAAGCCATGAAGAAGTTGCAAAACAATATCAAACGCCGCCCAGGCGCGATTGGTCGCGACCATGGCCAAAAGG
TCAAGGGTGGTGCTGCGGTTGCCGTCGCTGCAACTCCAGTAGCTGGCGCTGCGCCAGCCGCTGCTGCTAAGCCACGCGGC
CCTGTGGCAGCAATTGGCAAAAACATGCCAGAAGAAAAAGTTGCCAAGCTGATTTCAATTCGTGCTGCCAACGCTGCTAA
GCGTGGTGGTGGCGATGCTGGTGCTGCACCAGTCGCTGAAGCTGCGCCAATTGTGGAAACAGTTGTCGATACGCCAGCAG
CCAGCGCCGCACCAAGTGGCCCAGTTGCGGCAATTGGTCAGAACATGTCGGAAGACAAACGTGCCAAGCTTGAGGCAATT
CGCGCTGCGAAACGTGCCCAACGAGGAGAATAA

Upstream 100 bases:

>100_bases
TCAACCTTTCATCGCTGGCTGATATGTGCAAAGGCTACTCGATTGCCGACGTTGTGGTTATCTTGGGTAGTATCGATATC
GTGATGGGCGAGGTAGACCG

Downstream 100 bases:

>100_bases
TCGATGGCTGATGCACTAACACAACTAGGCTTTGCATCATGGCTGAGTGTTCTGATTGCCAGCATCATTAAAGTAACCCT
CATTCTCGTGGGCGGTTCGG

Product: 4Fe-4S ferredoxin iron-sulfur binding domain-containing protein

Products: NA

Alternate protein names: NADH dehydrogenase I subunit I 2; NDH-1 subunit I 2 [H]

Number of amino acids: Translated: 330; Mature: 329

Protein sequence:

>330_residues
MTLSHLATEDVFVVERNDADFRAGQGMTKGLGIVFRKFRKALFGKPNSDRADTEGQFNVQYPEERLVLPEAYRNMPILLY
DDATGHELCTSCFQCERICPPQVIHITQAKDPATGKPVPAAANFVLEYDTCMSCGYCAEVCPFDAIKMDHDFELSTGNHP
ELDVNYDRLLRPISYYEAIAPTMWSDVKEQAMKKLQNNIKRRPGAIGRDHGQKVKGGAAVAVAATPVAGAAPAAAAKPRG
PVAAIGKNMPEEKVAKLISIRAANAAKRGGGDAGAAPVAEAAPIVETVVDTPAASAAPSGPVAAIGQNMSEDKRAKLEAI
RAAKRAQRGE

Sequences:

>Translated_330_residues
MTLSHLATEDVFVVERNDADFRAGQGMTKGLGIVFRKFRKALFGKPNSDRADTEGQFNVQYPEERLVLPEAYRNMPILLY
DDATGHELCTSCFQCERICPPQVIHITQAKDPATGKPVPAAANFVLEYDTCMSCGYCAEVCPFDAIKMDHDFELSTGNHP
ELDVNYDRLLRPISYYEAIAPTMWSDVKEQAMKKLQNNIKRRPGAIGRDHGQKVKGGAAVAVAATPVAGAAPAAAAKPRG
PVAAIGKNMPEEKVAKLISIRAANAAKRGGGDAGAAPVAEAAPIVETVVDTPAASAAPSGPVAAIGQNMSEDKRAKLEAI
RAAKRAQRGE
>Mature_329_residues
TLSHLATEDVFVVERNDADFRAGQGMTKGLGIVFRKFRKALFGKPNSDRADTEGQFNVQYPEERLVLPEAYRNMPILLYD
DATGHELCTSCFQCERICPPQVIHITQAKDPATGKPVPAAANFVLEYDTCMSCGYCAEVCPFDAIKMDHDFELSTGNHPE
LDVNYDRLLRPISYYEAIAPTMWSDVKEQAMKKLQNNIKRRPGAIGRDHGQKVKGGAAVAVAATPVAGAAPAAAAKPRGP
VAAIGKNMPEEKVAKLISIRAANAAKRGGGDAGAAPVAEAAPIVETVVDTPAASAAPSGPVAAIGQNMSEDKRAKLEAIR
AAKRAQRGE

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1143

COG function: function code C; Formate hydrogenlyase subunit 6/NADH:ubiquinone oxidoreductase 23 kD subunit (chain I)

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Homo sapiens, GI4505371, Length=152, Percent_Identity=32.2368421052632, Blast_Score=65, Evalue=7e-11,
Organism=Escherichia coli, GI1788617, Length=108, Percent_Identity=35.1851851851852, Blast_Score=62, Evalue=4e-11,
Organism=Drosophila melanogaster, GI17864306, Length=104, Percent_Identity=37.5, Blast_Score=66, Evalue=4e-11,

Paralogues:

None

Copy number: 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001450
- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR010226 [H]

Pfam domain/function: PF00037 Fer4 [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 35286; Mature: 35154

Theoretical pI: Translated: 7.73; Mature: 7.73

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLSHLATEDVFVVERNDADFRAGQGMTKGLGIVFRKFRKALFGKPNSDRADTEGQFNVQ
CCCHHHCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEE
YPEERLVLPEAYRNMPILLYDDATGHELCTSCFQCERICPPQVIHITQAKDPATGKPVPA
CCHHHEECCHHHCCCCEEEEECCCCHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCH
AANFVLEYDTCMSCGYCAEVCPFDAIKMDHDFELSTGNHPELDVNYDRLLRPISYYEAIA
HHHHEEEHHHHHCCCCHHHHCCCCCEECCCCEEECCCCCCCCCCCHHHHHHHHHHHHHHH
PTMWSDVKEQAMKKLQNNIKRRPGAIGRDHGQKVKGGAAVAVAATPVAGAAPAAAAKPRG
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCC
PVAAIGKNMPEEKVAKLISIRAANAAKRGGGDAGAAPVAEAAPIVETVVDTPAASAAPSG
CHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCC
PVAAIGQNMSEDKRAKLEAIRAAKRAQRGE
CHHHHCCCCCHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TLSHLATEDVFVVERNDADFRAGQGMTKGLGIVFRKFRKALFGKPNSDRADTEGQFNVQ
CCHHHCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEE
YPEERLVLPEAYRNMPILLYDDATGHELCTSCFQCERICPPQVIHITQAKDPATGKPVPA
CCHHHEECCHHHCCCCEEEEECCCCHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCH
AANFVLEYDTCMSCGYCAEVCPFDAIKMDHDFELSTGNHPELDVNYDRLLRPISYYEAIA
HHHHEEEHHHHHCCCCHHHHCCCCCEECCCCEEECCCCCCCCCCCHHHHHHHHHHHHHHH
PTMWSDVKEQAMKKLQNNIKRRPGAIGRDHGQKVKGGAAVAVAATPVAGAAPAAAAKPRG
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCC
PVAAIGKNMPEEKVAKLISIRAANAAKRGGGDAGAAPVAEAAPIVETVVDTPAASAAPSG
CHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCC
PVAAIGQNMSEDKRAKLEAIRAAKRAQRGE
CHHHHCCCCCHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA