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The map label for this gene is rfbD [H]

Identifier: 159901331

GI number: 159901331

Start: 6146730

End: 6147758

Strand: Direct

Name: rfbD [H]

Synonym: Haur_4820

Alternate gene names: 159901331

Gene position: 6146730-6147758 (Clockwise)

Preceding gene: 159901326

Following gene: 159901332

Centisome position: 96.85

GC content: 52.48

Gene sequence:

>1029_bases
ATGCGAATAGGCATAAACAGCTTCTTGCCAACAATCTCAACTCGCAGTATAGTGCGGGCTATGACACGAGCATTAATTAC
TGGCGCTAATGGCTTTGTTGGCCAACATCTTGTTCGCTATTTGCAGCAAGCAACCACTTGGGAATTATGGGCTTTGGGGC
GTGAAGCTCACCCACAACTTCCAACAGTGCTGGCTGATCTGCTTGATCGTTCGGCGGTAGCAACGGCGGTGGCAAATGCA
GCCCCCGATCTGGTGGTGCATTTAGCGGCCCAATCGGCGATTCCTCAATCGTTTCGTGATCCCGCCGGCACGTTTAGTAT
CAATGTGCTCGGCCAATTGCACCTATTTGAAGCGATCAAGTCGGCTCAACTTGATCCAATTGTGTTGGTGGTTGGCTCGA
ATGCGATGTATGGCATGGCCCATCGTTCAGGCTTGCCCGCCGATGAAAACACCATGCTTTGCCCAGCTGATCCCTATGCT
GTTTCCAAGGCAGCCCAAGATCTGTTGGCGGGGCAATGGTGGTATAGCCATGGCCTGAAGGTGATTCGTGCTCGGCCATT
TAACCATACTGGGCCTGGCCAACGGGCTGATTTTGTTGTGCCAGCCTTTGCCCACCAAATTGCTCGCATCGAAGCGGGCT
TGCAGCCGCCAGTGATTCAGGTTGGCAACCTTACGCCACAGCGTGATTTTAGCGATGTACGTGATGTTGTGCGGGCCTAT
CATCTGCTGCTTGAACGAGCGCAGCCTGGCGAAATTTATAATATTGGCGTAGGTCAGAGTGTCTCAATTCAGTCAATTCT
TGATCGCCTCATTGCACTCAGTGGCCAAACGATCACGGTCGAAGTTGATCCTCAACGCTTGCGTCCAGTTGATGTGCCAA
TCGTGGCGTGCGATGCTAGTCGCTTGCGCAGCCAAATCGGGTGGGAGCCGCAGTATTGCCTTGACGATACGCTGAGCGAT
ATTCTGAATGAATGGCGCAGCCACGTTGCAACCGAGCAAGAGAGAGTTGGTTCCCACATTAACCAATAA

Upstream 100 bases:

>100_bases
GCTGCTAACCGCAACTGGAAGGTGAAATTGCTCGAACATTCAACGATCTCCAGCACAAATATTCAAAACGCTAGCCTCAG
TATAGAAGATTAAGCCCCCC

Downstream 100 bases:

>100_bases
CAGAGGATTAACCATATGGATATTTTAGTGCTTGCTGGGGGTACTGGCTCGCGATTGTGGCCGCGTTCACGTCGCACCAA
GCCCAAGCAATTTTTACCCT

Product: NAD-dependent epimerase/dehydratase

Products: NA

Alternate protein names: GDP-D-mannose dehydratase [H]

Number of amino acids: Translated: 342; Mature: 342

Protein sequence:

>342_residues
MRIGINSFLPTISTRSIVRAMTRALITGANGFVGQHLVRYLQQATTWELWALGREAHPQLPTVLADLLDRSAVATAVANA
APDLVVHLAAQSAIPQSFRDPAGTFSINVLGQLHLFEAIKSAQLDPIVLVVGSNAMYGMAHRSGLPADENTMLCPADPYA
VSKAAQDLLAGQWWYSHGLKVIRARPFNHTGPGQRADFVVPAFAHQIARIEAGLQPPVIQVGNLTPQRDFSDVRDVVRAY
HLLLERAQPGEIYNIGVGQSVSIQSILDRLIALSGQTITVEVDPQRLRPVDVPIVACDASRLRSQIGWEPQYCLDDTLSD
ILNEWRSHVATEQERVGSHINQ

Sequences:

>Translated_342_residues
MRIGINSFLPTISTRSIVRAMTRALITGANGFVGQHLVRYLQQATTWELWALGREAHPQLPTVLADLLDRSAVATAVANA
APDLVVHLAAQSAIPQSFRDPAGTFSINVLGQLHLFEAIKSAQLDPIVLVVGSNAMYGMAHRSGLPADENTMLCPADPYA
VSKAAQDLLAGQWWYSHGLKVIRARPFNHTGPGQRADFVVPAFAHQIARIEAGLQPPVIQVGNLTPQRDFSDVRDVVRAY
HLLLERAQPGEIYNIGVGQSVSIQSILDRLIALSGQTITVEVDPQRLRPVDVPIVACDASRLRSQIGWEPQYCLDDTLSD
ILNEWRSHVATEQERVGSHINQ
>Mature_342_residues
MRIGINSFLPTISTRSIVRAMTRALITGANGFVGQHLVRYLQQATTWELWALGREAHPQLPTVLADLLDRSAVATAVANA
APDLVVHLAAQSAIPQSFRDPAGTFSINVLGQLHLFEAIKSAQLDPIVLVVGSNAMYGMAHRSGLPADENTMLCPADPYA
VSKAAQDLLAGQWWYSHGLKVIRARPFNHTGPGQRADFVVPAFAHQIARIEAGLQPPVIQVGNLTPQRDFSDVRDVVRAY
HLLLERAQPGEIYNIGVGQSVSIQSILDRLIALSGQTITVEVDPQRLRPVDVPIVACDASRLRSQIGWEPQYCLDDTLSD
ILNEWRSHVATEQERVGSHINQ

Specific function: Biosynthesis of the slime polysaccharide colanic acid. First of the three steps in the biosynthesis of GDP-fucose from GDP-mannose. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GDP-mannose 4,6-dehydratase family [H]

Homologues:

Organism=Homo sapiens, GI4504031, Length=340, Percent_Identity=24.1176470588235, Blast_Score=101, Evalue=9e-22,
Organism=Homo sapiens, GI7657641, Length=327, Percent_Identity=24.7706422018349, Blast_Score=96, Evalue=6e-20,
Organism=Escherichia coli, GI1788366, Length=348, Percent_Identity=24.1379310344828, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI48994969, Length=333, Percent_Identity=27.3273273273273, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1788353, Length=342, Percent_Identity=26.6081871345029, Blast_Score=92, Evalue=6e-20,
Organism=Escherichia coli, GI1786974, Length=339, Percent_Identity=25.3687315634218, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1788589, Length=276, Percent_Identity=26.0869565217391, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI1788365, Length=314, Percent_Identity=25.1592356687898, Blast_Score=62, Evalue=5e-11,
Organism=Caenorhabditis elegans, GI133901786, Length=278, Percent_Identity=25.1798561151079, Blast_Score=102, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI17539424, Length=278, Percent_Identity=25.1798561151079, Blast_Score=102, Evalue=4e-22,
Organism=Caenorhabditis elegans, GI133901788, Length=278, Percent_Identity=25.1798561151079, Blast_Score=102, Evalue=4e-22,
Organism=Caenorhabditis elegans, GI17539422, Length=278, Percent_Identity=25.1798561151079, Blast_Score=102, Evalue=4e-22,
Organism=Caenorhabditis elegans, GI133901790, Length=278, Percent_Identity=25.1798561151079, Blast_Score=102, Evalue=4e-22,
Organism=Caenorhabditis elegans, GI17507723, Length=278, Percent_Identity=24.1007194244604, Blast_Score=97, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17568069, Length=340, Percent_Identity=24.7058823529412, Blast_Score=89, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI115532424, Length=335, Percent_Identity=23.2835820895522, Blast_Score=79, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI71982035, Length=345, Percent_Identity=23.1884057971014, Blast_Score=77, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI71982038, Length=347, Percent_Identity=23.0547550432277, Blast_Score=77, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17539532, Length=352, Percent_Identity=26.4204545454545, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24158427, Length=338, Percent_Identity=23.6686390532544, Blast_Score=105, Evalue=6e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR006368
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =4.2.1.47 [H]

Molecular weight: Translated: 37413; Mature: 37413

Theoretical pI: Translated: 6.74; Mature: 6.74

Prosite motif: PS00226 IF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIGINSFLPTISTRSIVRAMTRALITGANGFVGQHLVRYLQQATTWELWALGREAHPQL
CCCCHHHHHCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHCCCCCCCCH
PTVLADLLDRSAVATAVANAAPDLVVHLAAQSAIPQSFRDPAGTFSINVLGQLHLFEAIK
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCHHHCCCCCEEEEEHHHHHHHHHHHH
SAQLDPIVLVVGSNAMYGMAHRSGLPADENTMLCPADPYAVSKAAQDLLAGQWWYSHGLK
HCCCCCEEEEECCCCEECHHHHCCCCCCCCCEECCCCCHHHHHHHHHHHHHHHHHHCCCE
VIRARPFNHTGPGQRADFVVPAFAHQIARIEAGLQPPVIQVGNLTPQRDFSDVRDVVRAY
EEEECCCCCCCCCCCCCEEHHHHHHHHHHHHCCCCCCEEEECCCCCCCCHHHHHHHHHHH
HLLLERAQPGEIYNIGVGQSVSIQSILDRLIALSGQTITVEVDPQRLRPVDVPIVACDAS
HHHHHCCCCCCEEEECCCCCCCHHHHHHHHHHCCCCEEEEEECHHHCCCCCCCEEECCHH
RLRSQIGWEPQYCLDDTLSDILNEWRSHVATEQERVGSHINQ
HHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MRIGINSFLPTISTRSIVRAMTRALITGANGFVGQHLVRYLQQATTWELWALGREAHPQL
CCCCHHHHHCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHCCCCCCCCH
PTVLADLLDRSAVATAVANAAPDLVVHLAAQSAIPQSFRDPAGTFSINVLGQLHLFEAIK
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCHHHCCCCCEEEEEHHHHHHHHHHHH
SAQLDPIVLVVGSNAMYGMAHRSGLPADENTMLCPADPYAVSKAAQDLLAGQWWYSHGLK
HCCCCCEEEEECCCCEECHHHHCCCCCCCCCEECCCCCHHHHHHHHHHHHHHHHHHCCCE
VIRARPFNHTGPGQRADFVVPAFAHQIARIEAGLQPPVIQVGNLTPQRDFSDVRDVVRAY
EEEECCCCCCCCCCCCCEEHHHHHHHHHHHHCCCCCCEEEECCCCCCCCHHHHHHHHHHH
HLLLERAQPGEIYNIGVGQSVSIQSILDRLIALSGQTITVEVDPQRLRPVDVPIVACDAS
HHHHHCCCCCCEEEECCCCCCCHHHHHHHHHHCCCCEEEEEECHHHCCCCCCCEEECCHH
RLRSQIGWEPQYCLDDTLSDILNEWRSHVATEQERVGSHINQ
HHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9098074; 10521656 [H]