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The map label for this gene is aes [H]

Identifier: 159901227

GI number: 159901227

Start: 6024127

End: 6025017

Strand: Direct

Name: aes [H]

Synonym: Haur_4715

Alternate gene names: 159901227

Gene position: 6024127-6025017 (Clockwise)

Preceding gene: 159901226

Following gene: 159901230

Centisome position: 94.92

GC content: 48.71

Gene sequence:

>891_bases
ATGACAACCACTCCAACTCGTGCTCAACATATCAAAAAAGCCCAACGTTTTGCCAATGTACAGCGTTTAGTAAGCACAAT
TCAACGCTTTTTCTATCGTTTCAAACGTTCGGTTGGCCGCCAAGAGCTATCCATTGCAACTGAATTTGGCAAGGTTCGGG
TGCTCGGCTATGGCTTAGAAAATCCCAATCTAAGCCCAATTCTGTTTGATTTGCATGGTGGTGGCTTTATTTTGGGTAGT
GCCGATATGGACGAAGCCATGAATGTGCATTTTAAACAGCAAGTTGGCTGTAAAGTGATCAGCATCGATTATGCCAAAGC
GCCCAAATTTCCCTATCCAACGGCGGTTAATCAGGTGTATGCGGTGGTCAAATATTTTTATGAAAACGCTGAGAGCTATG
CGATTGATCGCGATCTGATGGCGATTGGTGGGCATAGTGCTGGCGGCAATTTAAGCGCGGTAACCTGCCTCAAAGCCAAG
CACGAAGGTCAATTTCAATTTGTCTGCCAAGTGCTCGATTATCCACCGTTGGATCTTGCAACCAGCCCGTTTGCTAAGCC
CAATCCCAAGGGTGCAATTCCACCGCAGATGGCGGCAATGTTCGATGATTGCTATCGCGAAGCCAGCCAAGCCAAGCATC
CATATGTCTCGCCTGTGTATGCCGAGCCAGCCGATTTGGTAGGCTTGCCGCCAGCCTTGTTTATTCTGGCGGGGCGCGAT
TCGCTACATGACGAGGCCTTGCGCTACGAGCAAATGCTCAAAAACGCTGGAGTACTCACCGAATGCTACGATTACCCTGA
GGCGGCCCACGGCTTTACCTACAAAACTTCAGCCGATACAACCGATGCGATCGCCAAAATGGTGGCATTTTTGCAGAAAT
ATTTACGCTGA

Upstream 100 bases:

>100_bases
GTAGGACTCCTATGGCCCAATACTATTTAGGTATTTCGGATTTTATGCTAGTGCTAAAATAGCTCCTGATTAATCGATTT
ATGCAGAAGGGTGGCTTCCA

Downstream 100 bases:

>100_bases
TGCATAAGCCTCGCACCTCCGACCCGCAGTGGGAGAGGGGTCGGAGAGCCTTACAAGGGCAGATTTTAAGCTCTCTCACT
CCCTGCCCCCTCTCCTACTG

Product: alpha/beta hydrolase domain-containing protein

Products: alcohol; a carboxylate [C]

Alternate protein names: NA

Number of amino acids: Translated: 296; Mature: 295

Protein sequence:

>296_residues
MTTTPTRAQHIKKAQRFANVQRLVSTIQRFFYRFKRSVGRQELSIATEFGKVRVLGYGLENPNLSPILFDLHGGGFILGS
ADMDEAMNVHFKQQVGCKVISIDYAKAPKFPYPTAVNQVYAVVKYFYENAESYAIDRDLMAIGGHSAGGNLSAVTCLKAK
HEGQFQFVCQVLDYPPLDLATSPFAKPNPKGAIPPQMAAMFDDCYREASQAKHPYVSPVYAEPADLVGLPPALFILAGRD
SLHDEALRYEQMLKNAGVLTECYDYPEAAHGFTYKTSADTTDAIAKMVAFLQKYLR

Sequences:

>Translated_296_residues
MTTTPTRAQHIKKAQRFANVQRLVSTIQRFFYRFKRSVGRQELSIATEFGKVRVLGYGLENPNLSPILFDLHGGGFILGS
ADMDEAMNVHFKQQVGCKVISIDYAKAPKFPYPTAVNQVYAVVKYFYENAESYAIDRDLMAIGGHSAGGNLSAVTCLKAK
HEGQFQFVCQVLDYPPLDLATSPFAKPNPKGAIPPQMAAMFDDCYREASQAKHPYVSPVYAEPADLVGLPPALFILAGRD
SLHDEALRYEQMLKNAGVLTECYDYPEAAHGFTYKTSADTTDAIAKMVAFLQKYLR
>Mature_295_residues
TTTPTRAQHIKKAQRFANVQRLVSTIQRFFYRFKRSVGRQELSIATEFGKVRVLGYGLENPNLSPILFDLHGGGFILGSA
DMDEAMNVHFKQQVGCKVISIDYAKAPKFPYPTAVNQVYAVVKYFYENAESYAIDRDLMAIGGHSAGGNLSAVTCLKAKH
EGQFQFVCQVLDYPPLDLATSPFAKPNPKGAIPPQMAAMFDDCYREASQAKHPYVSPVYAEPADLVGLPPALFILAGRDS
LHDEALRYEQMLKNAGVLTECYDYPEAAHGFTYKTSADTTDAIAKMVAFLQKYLR

Specific function: Displays esterase activity towards short chain fatty esters (acyl chain length of up to 8 carbons). Able to hydrolyze triacetylglycerol (triacetin) and tributyrylglycerol (tributyrin), but not trioleylglycerol (triolein) or cholesterol oleate. Negatively

COG id: COG0657

COG function: function code I; Esterase/lipase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'GDXG' lipolytic enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1786682, Length=145, Percent_Identity=31.0344827586207, Blast_Score=75, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI17567059, Length=296, Percent_Identity=29.7297297297297, Blast_Score=107, Evalue=6e-24,
Organism=Caenorhabditis elegans, GI72001146, Length=124, Percent_Identity=33.0645161290323, Blast_Score=69, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24656084, Length=115, Percent_Identity=32.1739130434783, Blast_Score=69, Evalue=4e-12,
Organism=Drosophila melanogaster, GI24656076, Length=115, Percent_Identity=32.1739130434783, Blast_Score=69, Evalue=4e-12,
Organism=Drosophila melanogaster, GI20130169, Length=115, Percent_Identity=32.1739130434783, Blast_Score=69, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013094
- InterPro:   IPR002168 [H]

Pfam domain/function: PF07859 Abhydrolase_3 [H]

EC number: 3.1.1.- [C]

Molecular weight: Translated: 32871; Mature: 32740

Theoretical pI: Translated: 8.19; Mature: 8.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTTPTRAQHIKKAQRFANVQRLVSTIQRFFYRFKRSVGRQELSIATEFGKVRVLGYGLE
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHCCCEEEEEECCC
NPNLSPILFDLHGGGFILGSADMDEAMNVHFKQQVGCKVISIDYAKAPKFPYPTAVNQVY
CCCCCEEEEEECCCEEEEECCCHHHHHCCHHHHHCCCEEEEEECCCCCCCCCCHHHHHHH
AVVKYFYENAESYAIDRDLMAIGGHSAGGNLSAVTCLKAKHEGQFQFVCQVLDYPPLDLA
HHHHHHHHCHHHHHHCHHHHEECCCCCCCCCCEEEEEHHCCCCHHHHHHHHHCCCCCCCC
TSPFAKPNPKGAIPPQMAAMFDDCYREASQAKHPYVSPVYAEPADLVGLPPALFILAGRD
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHCCCCHHHEEEECCC
SLHDEALRYEQMLKNAGVLTECYDYPEAAHGFTYKTSADTTDAIAKMVAFLQKYLR
CHHHHHHHHHHHHHHCCCHHHHHCCCCHHCCCEEECCCCHHHHHHHHHHHHHHHHC
>Mature Secondary Structure 
TTTPTRAQHIKKAQRFANVQRLVSTIQRFFYRFKRSVGRQELSIATEFGKVRVLGYGLE
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHCCCEEEEEECCC
NPNLSPILFDLHGGGFILGSADMDEAMNVHFKQQVGCKVISIDYAKAPKFPYPTAVNQVY
CCCCCEEEEEECCCEEEEECCCHHHHHCCHHHHHCCCEEEEEECCCCCCCCCCHHHHHHH
AVVKYFYENAESYAIDRDLMAIGGHSAGGNLSAVTCLKAKHEGQFQFVCQVLDYPPLDLA
HHHHHHHHCHHHHHHCHHHHEECCCCCCCCCCEEEEEHHCCCCHHHHHHHHHCCCCCCCC
TSPFAKPNPKGAIPPQMAAMFDDCYREASQAKHPYVSPVYAEPADLVGLPPALFILAGRD
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHCCCCHHHEEEECCC
SLHDEALRYEQMLKNAGVLTECYDYPEAAHGFTYKTSADTTDAIAKMVAFLQKYLR
CHHHHHHHHHHHHHHCCCHHHHHCCCCHHCCCEEECCCCHHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Carboxylic ester; H2O [C]

Specific reaction: Carboxylic ester + H2O = an alcohol + carboxylate [C]

General reaction: Hydrolase; Acting on ester bonds; Carboxylic ester hydrolases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA