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The map label for this gene is ybjS [C]

Identifier: 159901211

GI number: 159901211

Start: 6003683

End: 6004705

Strand: Direct

Name: ybjS [C]

Synonym: Haur_4699

Alternate gene names: 159901211

Gene position: 6003683-6004705 (Clockwise)

Preceding gene: 159901210

Following gene: 159901212

Centisome position: 94.6

GC content: 53.37

Gene sequence:

>1023_bases
ATGGCGCTTGAGCAATCCACACGGGCATTGGTGATTGGTGGTTGTGGCTTTGTCGGCAAACATTTGGTGCAGCAATTATT
GGCAGCAGGCCACCCTGTCCGCGTTTTCGATCTCCAGCCCTACCCCGACCCTCAAGTCGAATCGGTTGTAGGCGATTTAC
GCAAAGCTGAGCAGGTATTGCAAGCCTGTCACGATGTTGGTACGGTGTTTTTGTGTGCAGCAGCGGTCGATTGGGGTTGG
GGCAATGCCCAGCTCCTGCACGATGTCAATGTGTTGGGGCCGCAACATGTGGTTGCTGCTTGCCAAGCCACAGGTGTTGC
CCAACTGATTTATACCAGCAGCGTTGATGTGGTTTTCGAGGGCAAACCAATTCGGGCTGGCGATGAGCAATTGCCCTATC
CCAAGCAGCACTTAGATATTTATGGCGCGACTAAAACCGCTGGTGAACGTTTGGTTTTGGCGGCCAATGGTCAAGCAGGC
TTGGCAACCAGTGCCTTGCGATTGGGGGGCGTGTATGGCCCAGGCGATTCGCATCGCTTGCCATCGTTGGTGAATTTAGG
CAAGCGTGGCCCAATTCCACGTTTGGGCAATGGCTCGGCGCGATTTTCGCATATTTATGTTGAAAATGCCGCGCACGGCC
ATATTTTAGCAGCCCAGCGTTTAACCGCCGATGGTGCGATGGGCGGCCAAGCCTATTTTTTGGTTGACCCTAATCCTGAT
AACTTTTTTCTGTTTCTCAAGCCGATTGTTGAGGCCTTGGGTTTGCGCATGGCCAAGCGCCATGTGCCATTTGGCTTGAT
GCATTTCCTGGCATGGCCCAGCGAATTCTGGTATCGCACAACGCGCAGCAAAACCCGCCCAAGCCTAACGCGCTACACAG
TTACCTCAACCTGCGTTGATTTTTGGTTTACCGGAGCCAAGGCCGCCAACGATTTTGGCTATCAGCCGCTGGTTGATTTG
GCCGAAGCGCGGCAACGCACAATTGCATGGGCCAAACGTGAGTTTAATCTAGGCATGAAGTAA

Upstream 100 bases:

>100_bases
TGGTTGGCTCGGGCATTGATGTTTCGCGCTTGCCAGCCTTCGCTACTTTAGAACAAGCATTGAATTAGTTGCCGCATCGT
TGTAGTTGGGGGAATGGTCG

Downstream 100 bases:

>100_bases
CATCAAAAGTCAGAAATCAAAAATATAACTGTGGGGCGATGGGCTAGCTGAGATTATTGATAAGCTAATCGACTCATCGC
CTATAGCCAAAAGTCAATAG

Product: 3-beta hydroxysteroid dehydrogenase/isomerase

Products: NA

Alternate protein names: Cholesterol dehydrogenase; 3-beta-hydroxy-Delta(5)-steroid dehydrogenase; 3-beta-HSD; 3BHSD; 3-beta hydroxysterol dehydrogenase; 3-beta-hydroxy-5-ene steroid dehydrogenase; Progesterone reductase; Steroid Delta-isomerase; Delta-5-3-ketosteroid isomerase [H]

Number of amino acids: Translated: 340; Mature: 339

Protein sequence:

>340_residues
MALEQSTRALVIGGCGFVGKHLVQQLLAAGHPVRVFDLQPYPDPQVESVVGDLRKAEQVLQACHDVGTVFLCAAAVDWGW
GNAQLLHDVNVLGPQHVVAACQATGVAQLIYTSSVDVVFEGKPIRAGDEQLPYPKQHLDIYGATKTAGERLVLAANGQAG
LATSALRLGGVYGPGDSHRLPSLVNLGKRGPIPRLGNGSARFSHIYVENAAHGHILAAQRLTADGAMGGQAYFLVDPNPD
NFFLFLKPIVEALGLRMAKRHVPFGLMHFLAWPSEFWYRTTRSKTRPSLTRYTVTSTCVDFWFTGAKAANDFGYQPLVDL
AEARQRTIAWAKREFNLGMK

Sequences:

>Translated_340_residues
MALEQSTRALVIGGCGFVGKHLVQQLLAAGHPVRVFDLQPYPDPQVESVVGDLRKAEQVLQACHDVGTVFLCAAAVDWGW
GNAQLLHDVNVLGPQHVVAACQATGVAQLIYTSSVDVVFEGKPIRAGDEQLPYPKQHLDIYGATKTAGERLVLAANGQAG
LATSALRLGGVYGPGDSHRLPSLVNLGKRGPIPRLGNGSARFSHIYVENAAHGHILAAQRLTADGAMGGQAYFLVDPNPD
NFFLFLKPIVEALGLRMAKRHVPFGLMHFLAWPSEFWYRTTRSKTRPSLTRYTVTSTCVDFWFTGAKAANDFGYQPLVDL
AEARQRTIAWAKREFNLGMK
>Mature_339_residues
ALEQSTRALVIGGCGFVGKHLVQQLLAAGHPVRVFDLQPYPDPQVESVVGDLRKAEQVLQACHDVGTVFLCAAAVDWGWG
NAQLLHDVNVLGPQHVVAACQATGVAQLIYTSSVDVVFEGKPIRAGDEQLPYPKQHLDIYGATKTAGERLVLAANGQAGL
ATSALRLGGVYGPGDSHRLPSLVNLGKRGPIPRLGNGSARFSHIYVENAAHGHILAAQRLTADGAMGGQAYFLVDPNPDN
FFLFLKPIVEALGLRMAKRHVPFGLMHFLAWPSEFWYRTTRSKTRPSLTRYTVTSTCVDFWFTGAKAANDFGYQPLVDLA
EARQRTIAWAKREFNLGMK

Specific function: 3-beta-HSD is a bifunctional enzyme, that catalyzes the oxidation and isomerization of cholesterol, pregnenolone, and dehydroepiandrosterone (DHEA) into cholest-4-en-3-one, progesterone, and androsterone, respectively [H]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 3-beta-HSD family [H]

Homologues:

Organism=Homo sapiens, GI193211614, Length=329, Percent_Identity=36.4741641337386, Blast_Score=209, Evalue=4e-54,
Organism=Homo sapiens, GI8393516, Length=329, Percent_Identity=36.4741641337386, Blast_Score=209, Evalue=4e-54,
Organism=Homo sapiens, GI116268111, Length=351, Percent_Identity=35.042735042735, Blast_Score=162, Evalue=4e-40,
Organism=Homo sapiens, GI239745448, Length=330, Percent_Identity=34.2424242424242, Blast_Score=141, Evalue=7e-34,
Organism=Homo sapiens, GI310132178, Length=330, Percent_Identity=34.2424242424242, Blast_Score=141, Evalue=9e-34,
Organism=Homo sapiens, GI310113012, Length=330, Percent_Identity=34.2424242424242, Blast_Score=141, Evalue=9e-34,
Organism=Homo sapiens, GI19923621, Length=363, Percent_Identity=34.7107438016529, Blast_Score=139, Evalue=4e-33,
Organism=Homo sapiens, GI4504507, Length=355, Percent_Identity=29.8591549295775, Blast_Score=116, Evalue=3e-26,
Organism=Homo sapiens, GI260763931, Length=354, Percent_Identity=29.3785310734463, Blast_Score=112, Evalue=4e-25,
Organism=Homo sapiens, GI4504509, Length=354, Percent_Identity=29.3785310734463, Blast_Score=112, Evalue=4e-25,
Organism=Homo sapiens, GI218563686, Length=167, Percent_Identity=37.125748502994, Blast_Score=82, Evalue=1e-15,
Organism=Homo sapiens, GI218563684, Length=167, Percent_Identity=37.125748502994, Blast_Score=82, Evalue=1e-15,
Organism=Escherichia coli, GI87081792, Length=334, Percent_Identity=24.5508982035928, Blast_Score=86, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI17570557, Length=337, Percent_Identity=27.893175074184, Blast_Score=115, Evalue=3e-26,
Organism=Caenorhabditis elegans, GI193211272, Length=334, Percent_Identity=26.3473053892216, Blast_Score=97, Evalue=9e-21,
Organism=Caenorhabditis elegans, GI17509709, Length=235, Percent_Identity=29.3617021276596, Blast_Score=97, Evalue=9e-21,
Organism=Saccharomyces cerevisiae, GI6321437, Length=350, Percent_Identity=29.1428571428571, Blast_Score=147, Evalue=2e-36,
Organism=Drosophila melanogaster, GI21355349, Length=375, Percent_Identity=28.2666666666667, Blast_Score=95, Evalue=6e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: =1.1.1.145; =5.3.3.1 [H]

Molecular weight: Translated: 36969; Mature: 36838

Theoretical pI: Translated: 8.91; Mature: 8.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALEQSTRALVIGGCGFVGKHLVQQLLAAGHPVRVFDLQPYPDPQVESVVGDLRKAEQVL
CCCCCCCCEEEEECCHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHH
QACHDVGTVFLCAAAVDWGWGNAQLLHDVNVLGPQHVVAACQATGVAQLIYTSSVDVVFE
HHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHHHCCCEEEEEECCCEEEEC
GKPIRAGDEQLPYPKQHLDIYGATKTAGERLVLAANGQAGLATSALRLGGVYGPGDSHRL
CCCCCCCCCCCCCCHHHEEEEECCCCCCCEEEEEECCCCCHHHHHHHHCCCCCCCCCCCC
PSLVNLGKRGPIPRLGNGSARFSHIYVENAAHGHILAAQRLTADGAMGGQAYFLVDPNPD
CHHHHCCCCCCCCCCCCCCCEEEEEEEECCCCCCEEEHHHCCCCCCCCCEEEEEECCCCC
NFFLFLKPIVEALGLRMAKRHVPFGLMHFLAWPSEFWYRTTRSKTRPSLTRYTVTSTCVD
CEEEEHHHHHHHHHHHHHHHCCCHHHHHHHHCCHHHHHHHCCCCCCCCCEEEEHHHHHHH
FWFTGAKAANDFGYQPLVDLAEARQRTIAWAKREFNLGMK
HHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
ALEQSTRALVIGGCGFVGKHLVQQLLAAGHPVRVFDLQPYPDPQVESVVGDLRKAEQVL
CCCCCCCEEEEECCHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHH
QACHDVGTVFLCAAAVDWGWGNAQLLHDVNVLGPQHVVAACQATGVAQLIYTSSVDVVFE
HHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHHHCCCEEEEEECCCEEEEC
GKPIRAGDEQLPYPKQHLDIYGATKTAGERLVLAANGQAGLATSALRLGGVYGPGDSHRL
CCCCCCCCCCCCCCHHHEEEEECCCCCCCEEEEEECCCCCHHHHHHHHCCCCCCCCCCCC
PSLVNLGKRGPIPRLGNGSARFSHIYVENAAHGHILAAQRLTADGAMGGQAYFLVDPNPD
CHHHHCCCCCCCCCCCCCCCEEEEEEEECCCCCCEEEHHHCCCCCCCCCEEEEEECCCCC
NFFLFLKPIVEALGLRMAKRHVPFGLMHFLAWPSEFWYRTTRSKTRPSLTRYTVTSTCVD
CEEEEHHHHHHHHHHHHHHHCCCHHHHHHHHCCHHHHHHHCCCCCCCCCEEEEHHHHHHH
FWFTGAKAANDFGYQPLVDLAEARQRTIAWAKREFNLGMK
HHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]