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The map label for this gene is alaXL [H]

Identifier: 159901144

GI number: 159901144

Start: 5918351

End: 5919514

Strand: Direct

Name: alaXL [H]

Synonym: Haur_4632

Alternate gene names: 159901144

Gene position: 5918351-5919514 (Clockwise)

Preceding gene: 159901141

Following gene: 159901146

Centisome position: 93.25

GC content: 53.52

Gene sequence:

>1164_bases
ATGAGCACTGAACGACTTTATTGGCACGATGCCTATTTGCGCGAATGGTCAGCCCAAATTGTAGCTCGCGATCAGCAACG
GGTGGCGCTTGATCGCAGCGCTTTTTATCCCGAAGGTGGTGGTCAGCCCGCTGATCATGGCTGGATTAACGGGGTGGCGG
TGGTCGATGTGCAGGCTGATGATCATGATTTGGTGTGGCATACCCTCGCTGCGCCAATTGATAGCGATAGCGTGGAATGT
CGCCTAGATTGGCAACGACGCTTCGATCATATGCAGCAACATCATGGCCAGCATTTGCTCTCGGCAGCATTTGATCAGCT
ATTCAATTGGCGCACAGTTGGTTTTCATCTCAGCAGCGAGTATGTCACAATTGATTTAGCCACTGGTGAAGCTGACTCCG
AGCAACTTGGCGAGGCTGAAACTCTCGCCAACCAAGTAATTTGGCAAAATTTGCCGATTTTGGCGCGATTTGTCAGCGCC
GAAGAACTTGCCACGCTGGCCTTGCGCAAAGCTCCCAGCGTAACTGGAGCAATTCGCGTGGTTTCGGCTGGCGATTTTGA
TCATTCAGCATGTGGCGGAACCCACCCCAATGCGACTGGCGCGGTTGGTCAGATTCATATTCGGCGCAGCGAAAAGCGTG
GCGAGAGTGTGCGAATTGAATTTGTGTGTGGTGGGCGAGCGTTGCAGGATTTGCGTTGGAAGAATGCTGCGCTTGGCCGA
ATCGCCGCAGGTTTTAGCGTGGCTCAACCTCAAGCCGAGCAAGCGATCGAACGGTTACGTGAGCAAGAACAACAAGTACG
CAAACAGTTTCAGCAGGCCGAGCAACAACTATTGGAGTTTGAAGCCAAAGCGTTGGTTGAGCAAGCTCAGCGGATTGGTC
AATTAAAGGTTGTGGCCCAAGTTTGGCTGCGCGAACCGCAAACCGTGCGCCAACTAGCCAATTTGGTGGCTCAGGCAGGC
GGGGTAGCCTTGTTTGGCGTGGCAGCTAGCAAACCCCAATTAATCTTTGCCGGCCAAGGCTTGGATTGCGGGGCAATTTT
GCGGCAGGTTGTGGCAGCATTTGGCGGCAAGGGCGGTGGTAGCGTTCAACAAGCCCAAGGTGGCATTGCTGAGGCCAACG
ATTTAGCCGCCGCCTTAGAGTTGGCAAAACAGCAAATTGCTTAA

Upstream 100 bases:

>100_bases
GTTTCTTTAGCGTAATACTCGGCGGCCTTACTCGTCATTGAGGTTCCTCCTTGACACAGATTGATCTATCATAGCATATT
CTGTTTTGCAGGAGCAGTTG

Downstream 100 bases:

>100_bases
ATAGCAAATGCAGGCATGCCGAATGTACAGCATGCCTGCATCGCTAAAATAGGTTAGCTGCGAATTTGCACGCCCAATTC
GCGTTCTAGCTGCTTGAGCA

Product: threonyl/alanyl tRNA synthetase SAD

Products: AMP; diphosphate; L-alanyl-tRNA(Ala)

Alternate protein names: AlaX-L; Alanyl-tRNA deacylase AlaX-L [H]

Number of amino acids: Translated: 387; Mature: 386

Protein sequence:

>387_residues
MSTERLYWHDAYLREWSAQIVARDQQRVALDRSAFYPEGGGQPADHGWINGVAVVDVQADDHDLVWHTLAAPIDSDSVEC
RLDWQRRFDHMQQHHGQHLLSAAFDQLFNWRTVGFHLSSEYVTIDLATGEADSEQLGEAETLANQVIWQNLPILARFVSA
EELATLALRKAPSVTGAIRVVSAGDFDHSACGGTHPNATGAVGQIHIRRSEKRGESVRIEFVCGGRALQDLRWKNAALGR
IAAGFSVAQPQAEQAIERLREQEQQVRKQFQQAEQQLLEFEAKALVEQAQRIGQLKVVAQVWLREPQTVRQLANLVAQAG
GVALFGVAASKPQLIFAGQGLDCGAILRQVVAAFGGKGGGSVQQAQGGIAEANDLAAALELAKQQIA

Sequences:

>Translated_387_residues
MSTERLYWHDAYLREWSAQIVARDQQRVALDRSAFYPEGGGQPADHGWINGVAVVDVQADDHDLVWHTLAAPIDSDSVEC
RLDWQRRFDHMQQHHGQHLLSAAFDQLFNWRTVGFHLSSEYVTIDLATGEADSEQLGEAETLANQVIWQNLPILARFVSA
EELATLALRKAPSVTGAIRVVSAGDFDHSACGGTHPNATGAVGQIHIRRSEKRGESVRIEFVCGGRALQDLRWKNAALGR
IAAGFSVAQPQAEQAIERLREQEQQVRKQFQQAEQQLLEFEAKALVEQAQRIGQLKVVAQVWLREPQTVRQLANLVAQAG
GVALFGVAASKPQLIFAGQGLDCGAILRQVVAAFGGKGGGSVQQAQGGIAEANDLAAALELAKQQIA
>Mature_386_residues
STERLYWHDAYLREWSAQIVARDQQRVALDRSAFYPEGGGQPADHGWINGVAVVDVQADDHDLVWHTLAAPIDSDSVECR
LDWQRRFDHMQQHHGQHLLSAAFDQLFNWRTVGFHLSSEYVTIDLATGEADSEQLGEAETLANQVIWQNLPILARFVSAE
ELATLALRKAPSVTGAIRVVSAGDFDHSACGGTHPNATGAVGQIHIRRSEKRGESVRIEFVCGGRALQDLRWKNAALGRI
AAGFSVAQPQAEQAIERLREQEQQVRKQFQQAEQQLLEFEAKALVEQAQRIGQLKVVAQVWLREPQTVRQLANLVAQAGG
VALFGVAASKPQLIFAGQGLDCGAILRQVVAAFGGKGGGSVQQAQGGIAEANDLAAALELAKQQIA

Specific function: Functions in trans to edit the amino acid moiety from mischarged charged tRNA(Ala) [H]

COG id: COG2872

COG function: function code R; Predicted metal-dependent hydrolases related to alanyl-tRNA synthetase HxxxH domain

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. Editing domain AlaX-L subfamily [H]

Homologues:

Organism=Homo sapiens, GI13376886, Length=261, Percent_Identity=34.0996168582375, Blast_Score=123, Evalue=3e-28,
Organism=Homo sapiens, GI217416402, Length=261, Percent_Identity=34.0996168582375, Blast_Score=122, Evalue=4e-28,
Organism=Escherichia coli, GI1789048, Length=367, Percent_Identity=28.3378746594005, Blast_Score=95, Evalue=7e-21,
Organism=Saccharomyces cerevisiae, GI6324288, Length=277, Percent_Identity=27.4368231046931, Blast_Score=96, Evalue=1e-20,
Organism=Drosophila melanogaster, GI18543305, Length=405, Percent_Identity=26.6666666666667, Blast_Score=111, Evalue=8e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018165
- InterPro:   IPR018164
- InterPro:   IPR003156
- InterPro:   IPR018163
- InterPro:   IPR012947 [H]

Pfam domain/function: PF02272 DHHA1; PF01411 tRNA-synt_2c; PF07973 tRNA_SAD [H]

EC number: 6.1.1.7

Molecular weight: Translated: 42254; Mature: 42123

Theoretical pI: Translated: 5.93; Mature: 5.93

Prosite motif: PS50860 AA_TRNA_LIGASE_II_ALA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
0.3 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTERLYWHDAYLREWSAQIVARDQQRVALDRSAFYPEGGGQPADHGWINGVAVVDVQAD
CCCCEEEEHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEEECC
DHDLVWHTLAAPIDSDSVECRLDWQRRFDHMQQHHGQHLLSAAFDQLFNWRTVGFHLSSE
CCCHHHHHHHCCCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCC
YVTIDLATGEADSEQLGEAETLANQVIWQNLPILARFVSAEELATLALRKAPSVTGAIRV
EEEEEEECCCCCHHHCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEE
VSAGDFDHSACGGTHPNATGAVGQIHIRRSEKRGESVRIEFVCGGRALQDLRWKNAALGR
EECCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCCEEEEEEEECCHHHHHHHHHHHHHHH
IAAGFSVAQPQAEQAIERLREQEQQVRKQFQQAEQQLLEFEAKALVEQAQRIGQLKVVAQ
HHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VWLREPQTVRQLANLVAQAGGVALFGVAASKPQLIFAGQGLDCGAILRQVVAAFGGKGGG
HHHCCCHHHHHHHHHHHHCCCEEEEEEECCCCCEEEECCCCCHHHHHHHHHHHHCCCCCC
SVQQAQGGIAEANDLAAALELAKQQIA
CHHHHCCCCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
STERLYWHDAYLREWSAQIVARDQQRVALDRSAFYPEGGGQPADHGWINGVAVVDVQAD
CCCEEEEHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEEECC
DHDLVWHTLAAPIDSDSVECRLDWQRRFDHMQQHHGQHLLSAAFDQLFNWRTVGFHLSSE
CCCHHHHHHHCCCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCC
YVTIDLATGEADSEQLGEAETLANQVIWQNLPILARFVSAEELATLALRKAPSVTGAIRV
EEEEEEECCCCCHHHCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEE
VSAGDFDHSACGGTHPNATGAVGQIHIRRSEKRGESVRIEFVCGGRALQDLRWKNAALGR
EECCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCCEEEEEEEECCHHHHHHHHHHHHHHH
IAAGFSVAQPQAEQAIERLREQEQQVRKQFQQAEQQLLEFEAKALVEQAQRIGQLKVVAQ
HHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VWLREPQTVRQLANLVAQAGGVALFGVAASKPQLIFAGQGLDCGAILRQVVAAFGGKGGG
HHHCCCHHHHHHHHHHHHCCCEEEEEEECCCCCEEEECCCCCHHHHHHHHHHHHCCCCCC
SVQQAQGGIAEANDLAAALELAKQQIA
CHHHHCCCCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-alanine; tRNA(Ala)

Specific reaction: ATP + L-alanine + tRNA(Ala) = AMP + diphosphate + L-alanyl-tRNA(Ala)

General reaction: Aminoacylation; Esterification [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9679194 [H]