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The map label for this gene is dam [C]

Identifier: 159901051

GI number: 159901051

Start: 5810405

End: 5811199

Strand: Reverse

Name: dam [C]

Synonym: Haur_4539

Alternate gene names: 159901051

Gene position: 5811199-5810405 (Counterclockwise)

Preceding gene: 159901052

Following gene: 159901050

Centisome position: 91.56

GC content: 46.92

Gene sequence:

>795_bases
ATGCTGACCCAAGCTCTACCACTGCTCAATCCACCGCTCAAATGGGCTGGTGGTAAACGCTGGCTAATTCCCCGCCTGAG
CGATTTTTGGCAGAACTATCAAGATTATACCTTGGTTGAGCCATTTGTTGGTGGTTTGGCGATTGCGTTGGGTTTGCAAC
CACAACAGGCACTTTTGAATGATATTAATCCACATGTGATTAATTTTTATCACTGGTTGCAGCATGGCTTGGTGATCGAT
TTTGAATTACATAACGACCAAACTTATTATTATCGTTATCGTGACCAATTCAATCAATTAATTCGTGCTGGGGCTGAGCA
AACTGCTGAGGCTGCTGGGTTGTTTTATTATCTCAATCGCACGGGCTATAATGGGCTGTGTCGTTTTAATCAGCGTGGCT
TGTTTAATGTGCCATTTGGGCGCTACAAACGCATTCGTTATTGTTATGATTTTAGCCATTATGCCGAAACCTTGCGCCAA
TGGCGGTTTCAGCGGGGCCATTTTATTGAAATGCAAATCCCGGCTCAAGCCTTAATTTATGCCGACCCACCCTACGATGT
GCCATTTCGCGCCTATAGCCAGCAAGGATTTAATTGGAGCGAACAGGTGCAATTGGCTGAATGGCTAGCGCAACACCCTG
GCCCGGTGCTGGCATCGAATCAAGCAACCCAACGTATTTTAGATTTGTATCGCAATTTGGGCTTTTCAATCGAGATCTTG
CCTGCACCACGGCGTATCGCCTGCAACGGCGACCGTCAACCAGCGCTCGAAATGCTGGCCTTCAAAGGAGCTTGA

Upstream 100 bases:

>100_bases
TGCCGAATTTCGCGATTTTCGACTCGAAGCACTCGCGCAGGAATAGCATGTCTGTGTCAGTATGGTATAGTTGGGCAAAT
TATCGACCAACAGGAAGCCT

Downstream 100 bases:

>100_bases
GATGGCTGTTGTGCTCAAACCACCAACTGCTTTGCCCGCCACCAAAACCATGCCCACGATTTTTTTGGCCGGCTCGATCG
ATATGGGCAGTGCAATCGAT

Product: DNA adenine methylase

Products: NA

Alternate protein names: M.Eco67Dam; ORF1-EC67 DAM [H]

Number of amino acids: Translated: 264; Mature: 264

Protein sequence:

>264_residues
MLTQALPLLNPPLKWAGGKRWLIPRLSDFWQNYQDYTLVEPFVGGLAIALGLQPQQALLNDINPHVINFYHWLQHGLVID
FELHNDQTYYYRYRDQFNQLIRAGAEQTAEAAGLFYYLNRTGYNGLCRFNQRGLFNVPFGRYKRIRYCYDFSHYAETLRQ
WRFQRGHFIEMQIPAQALIYADPPYDVPFRAYSQQGFNWSEQVQLAEWLAQHPGPVLASNQATQRILDLYRNLGFSIEIL
PAPRRIACNGDRQPALEMLAFKGA

Sequences:

>Translated_264_residues
MLTQALPLLNPPLKWAGGKRWLIPRLSDFWQNYQDYTLVEPFVGGLAIALGLQPQQALLNDINPHVINFYHWLQHGLVID
FELHNDQTYYYRYRDQFNQLIRAGAEQTAEAAGLFYYLNRTGYNGLCRFNQRGLFNVPFGRYKRIRYCYDFSHYAETLRQ
WRFQRGHFIEMQIPAQALIYADPPYDVPFRAYSQQGFNWSEQVQLAEWLAQHPGPVLASNQATQRILDLYRNLGFSIEIL
PAPRRIACNGDRQPALEMLAFKGA
>Mature_264_residues
MLTQALPLLNPPLKWAGGKRWLIPRLSDFWQNYQDYTLVEPFVGGLAIALGLQPQQALLNDINPHVINFYHWLQHGLVID
FELHNDQTYYYRYRDQFNQLIRAGAEQTAEAAGLFYYLNRTGYNGLCRFNQRGLFNVPFGRYKRIRYCYDFSHYAETLRQ
WRFQRGHFIEMQIPAQALIYADPPYDVPFRAYSQQGFNWSEQVQLAEWLAQHPGPVLASNQATQRILDLYRNLGFSIEIL
PAPRRIACNGDRQPALEMLAFKGA

Specific function: Methylates DNA within the sequence GATC and protects the DNA from cleavage by the restriction endonuclease MboI. May play a regulatory role in the functions of the retron [H]

COG id: COG0338

COG function: function code L; Site-specific DNA methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789789, Length=270, Percent_Identity=33.7037037037037, Blast_Score=118, Evalue=4e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023095
- InterPro:   IPR002052
- InterPro:   IPR012327 [H]

Pfam domain/function: PF02086 MethyltransfD12 [H]

EC number: =2.1.1.72 [H]

Molecular weight: Translated: 30870; Mature: 30870

Theoretical pI: Translated: 8.68; Mature: 8.68

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTQALPLLNPPLKWAGGKRWLIPRLSDFWQNYQDYTLVEPFVGGLAIALGLQPQQALLN
CCCCCCCCCCCCCCCCCCCEEECCCHHHHHCCCCCCCHHHHHHHHEEEEECCCHHHHHHH
DINPHVINFYHWLQHGLVIDFELHNDQTYYYRYRDQFNQLIRAGAEQTAEAAGLFYYLNR
CCCHHHHHHHHHHHCCEEEEEEECCCCEEEEHHHHHHHHHHHHCHHHHHHHHHEEEEEEC
TGYNGLCRFNQRGLFNVPFGRYKRIRYCYDFSHYAETLRQWRFQRGHFIEMQIPAQALIY
CCCCCEEEECCCCCEECCCHHHHHHHHEECHHHHHHHHHHHHHCCCCEEEEECCCEEEEE
ADPPYDVPFRAYSQQGFNWSEQVQLAEWLAQHPGPVLASNQATQRILDLYRNLGFSIEIL
ECCCCCCCHHHHHCCCCCHHHHHHHHHHHHHCCCCEEECCHHHHHHHHHHHCCCCEEEEE
PAPRRIACNGDRQPALEMLAFKGA
CCCCEEEECCCCCHHHHHHHHCCC
>Mature Secondary Structure
MLTQALPLLNPPLKWAGGKRWLIPRLSDFWQNYQDYTLVEPFVGGLAIALGLQPQQALLN
CCCCCCCCCCCCCCCCCCCEEECCCHHHHHCCCCCCCHHHHHHHHEEEEECCCHHHHHHH
DINPHVINFYHWLQHGLVIDFELHNDQTYYYRYRDQFNQLIRAGAEQTAEAAGLFYYLNR
CCCHHHHHHHHHHHCCEEEEEEECCCCEEEEHHHHHHHHHHHHCHHHHHHHHHEEEEEEC
TGYNGLCRFNQRGLFNVPFGRYKRIRYCYDFSHYAETLRQWRFQRGHFIEMQIPAQALIY
CCCCCEEEECCCCCEECCCHHHHHHHHEECHHHHHHHHHHHHHCCCCEEEEECCCEEEEE
ADPPYDVPFRAYSQQGFNWSEQVQLAEWLAQHPGPVLASNQATQRILDLYRNLGFSIEIL
ECCCCCCCHHHHHCCCCCHHHHHHHHHHHHHCCCCEEECCHHHHHHHHHHHCCCCEEEEE
PAPRRIACNGDRQPALEMLAFKGA
CCCCEEEECCCCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1701261 [H]