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The map label for this gene is rimO

Identifier: 159901008

GI number: 159901008

Start: 5755981

End: 5757390

Strand: Reverse

Name: rimO

Synonym: Haur_4495

Alternate gene names: 159901008

Gene position: 5757390-5755981 (Counterclockwise)

Preceding gene: 159901010

Following gene: 159901006

Centisome position: 90.72

GC content: 54.11

Gene sequence:

>1410_bases
ATGAAGTTTCATATCATTACTTTAGGCTGTCCGAAAAATACCGTCGATAGCGAAGGCATGCATGGCATTCTGACGCGCGA
AGGCCACACCGCCGTCGATAGCAGCGATGGCGCTGACGTGGTGATCGTCAATACCTGTTCGTTTATCAACGCTGCCCGCG
AAGAAACCGTGGGCGTGTTGCAAGAGCTGGCCAACAACAAAGCGCCAGGCCAGAAATTAATTGCGGCTGGCTGTATGGCC
GAAAGCCATGGCGATGTCTTACGTTCACGTGTGCCTAAGCTTGATGCCACCCTCAGCACCAAAGAATGGATGCGCATCGG
TTCAGTTGTGGCTGGCGGCGTTGCGCCTAGCAAAACCACAGGCTTTGGGATTCCCTTGATGGGCGGCGCACCAAGTGCAA
TGCCCAGCACAGGGCTTAATCTTAGCCTCACGCCTGCCGCAACTGGCGATAGCCTCGGAGCCTATGGCGATTGGCGCACA
ACCGCGATTACCCGCAACAAACGCGGCCCCTCGGCCTATCTCAAAATTTCCGATGGCTGTAATTTGCGCTGTGCCTTCTG
TACGATTCCTTCGTTCAAAGGCGATATGCGCTCAAAAGCGGTTGGCTCGATTTTAGGTGAAGCGCGTGAGTTGGTCGAGG
CTGGGGTGCAAGAAATTATTCTGGTTGCCCAGCACCTCACCGATTATGGCCGCGATTTGGGCATGAAACAAAATGGCCTG
GGGGTTTTGCTCGAAGAGTTAGCCGCAGTTGTACCAGCCGACCGCTGGATTCGGCTGATGTACGCGTATCCTCAATCGGT
TACGCCTGATTTGGTCGAAACCATGGCGCGGCTGCCACAATTGTGTCATTATGTCGATATGCCATTGCAGCATGCCCACC
CTGATACCTTGCGCCGCATGCGCCGCCCACCCGACACCGACAAAACCAAAGCAATTGTCAATTCGTTGCGCCAAGCCATG
CCCGATTTGTCGTTACGCACAACGTTTATTGTGGGCTATCCTGGCGAAACCCGCGATGAATTCAAAGCTTTACTCGAATT
CCTCGAAGAAATGCAATTTGATCGCGTTGGCATGTTCCGCTATTCATTGGAGCCAGGCACAGTTGCTGGCGAATTGCCCG
ATCAAGTCGCTGAGCGAGTCAAAGAGCGGCGTTGGAACGAAGCCATGGCGGTGCAACAAGTGATTTCGCGTGCTCGCACT
GCGCGGTTTGTCGGCCAAACCATGAAGGTATTGGTCGAAGGCACTGGCACTGATGATGATGGTCGGGCGATTGTGGTTGG
GCGTTCGTATCGCGATGCCCCCGAAGTTGATGGCTTGGTCTTTGGCTATGGCGCTGCCGACGTTGGCCAATTTGCCAAGA
TTGCCATCAACAAAACCACCGATTACGACTTGTGGGGCGAAATCGTTTAA

Upstream 100 bases:

>100_bases
GGAGTTCAATTACAGCCGAAGCTGAAACTTGCTGGTACAATAGACACATCGATCTTAATGTTTAATCAACGAGGCTTGCC
TCATCGAGTTTATACAACAC

Downstream 100 bases:

>100_bases
GCAACCCCTCGCCACTCAACCCGCGCTTCCGCCAAGCGAGGCGAGAGCCAGTAAACCTAAACTGCGCAAAAGCCCCTCGC
CCGCCGCAGTGGGAGAGGGG

Product: MiaB-like tRNA modifying protein YliG

Products: NA

Alternate protein names: S12 MTTase; S12 methylthiotransferase; Ribosome maturation factor RimO

Number of amino acids: Translated: 469; Mature: 469

Protein sequence:

>469_residues
MKFHIITLGCPKNTVDSEGMHGILTREGHTAVDSSDGADVVIVNTCSFINAAREETVGVLQELANNKAPGQKLIAAGCMA
ESHGDVLRSRVPKLDATLSTKEWMRIGSVVAGGVAPSKTTGFGIPLMGGAPSAMPSTGLNLSLTPAATGDSLGAYGDWRT
TAITRNKRGPSAYLKISDGCNLRCAFCTIPSFKGDMRSKAVGSILGEARELVEAGVQEIILVAQHLTDYGRDLGMKQNGL
GVLLEELAAVVPADRWIRLMYAYPQSVTPDLVETMARLPQLCHYVDMPLQHAHPDTLRRMRRPPDTDKTKAIVNSLRQAM
PDLSLRTTFIVGYPGETRDEFKALLEFLEEMQFDRVGMFRYSLEPGTVAGELPDQVAERVKERRWNEAMAVQQVISRART
ARFVGQTMKVLVEGTGTDDDGRAIVVGRSYRDAPEVDGLVFGYGAADVGQFAKIAINKTTDYDLWGEIV

Sequences:

>Translated_469_residues
MKFHIITLGCPKNTVDSEGMHGILTREGHTAVDSSDGADVVIVNTCSFINAAREETVGVLQELANNKAPGQKLIAAGCMA
ESHGDVLRSRVPKLDATLSTKEWMRIGSVVAGGVAPSKTTGFGIPLMGGAPSAMPSTGLNLSLTPAATGDSLGAYGDWRT
TAITRNKRGPSAYLKISDGCNLRCAFCTIPSFKGDMRSKAVGSILGEARELVEAGVQEIILVAQHLTDYGRDLGMKQNGL
GVLLEELAAVVPADRWIRLMYAYPQSVTPDLVETMARLPQLCHYVDMPLQHAHPDTLRRMRRPPDTDKTKAIVNSLRQAM
PDLSLRTTFIVGYPGETRDEFKALLEFLEEMQFDRVGMFRYSLEPGTVAGELPDQVAERVKERRWNEAMAVQQVISRART
ARFVGQTMKVLVEGTGTDDDGRAIVVGRSYRDAPEVDGLVFGYGAADVGQFAKIAINKTTDYDLWGEIV
>Mature_469_residues
MKFHIITLGCPKNTVDSEGMHGILTREGHTAVDSSDGADVVIVNTCSFINAAREETVGVLQELANNKAPGQKLIAAGCMA
ESHGDVLRSRVPKLDATLSTKEWMRIGSVVAGGVAPSKTTGFGIPLMGGAPSAMPSTGLNLSLTPAATGDSLGAYGDWRT
TAITRNKRGPSAYLKISDGCNLRCAFCTIPSFKGDMRSKAVGSILGEARELVEAGVQEIILVAQHLTDYGRDLGMKQNGL
GVLLEELAAVVPADRWIRLMYAYPQSVTPDLVETMARLPQLCHYVDMPLQHAHPDTLRRMRRPPDTDKTKAIVNSLRQAM
PDLSLRTTFIVGYPGETRDEFKALLEFLEEMQFDRVGMFRYSLEPGTVAGELPDQVAERVKERRWNEAMAVQQVISRART
ARFVGQTMKVLVEGTGTDDDGRAIVVGRSYRDAPEVDGLVFGYGAADVGQFAKIAINKTTDYDLWGEIV

Specific function: Catalyzes the methylthiolation of an aspartic acid residue of ribosomal protein S12

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain

Homologues:

Organism=Homo sapiens, GI28872784, Length=456, Percent_Identity=26.5350877192982, Blast_Score=135, Evalue=1e-31,
Organism=Homo sapiens, GI28872782, Length=455, Percent_Identity=26.1538461538462, Blast_Score=134, Evalue=2e-31,
Organism=Homo sapiens, GI93277076, Length=423, Percent_Identity=25.2955082742317, Blast_Score=124, Evalue=3e-28,
Organism=Escherichia coli, GI1787057, Length=476, Percent_Identity=38.2352941176471, Blast_Score=301, Evalue=6e-83,
Organism=Escherichia coli, GI1786882, Length=475, Percent_Identity=28.4210526315789, Blast_Score=177, Evalue=1e-45,
Organism=Caenorhabditis elegans, GI17553146, Length=503, Percent_Identity=26.6401590457256, Blast_Score=149, Evalue=2e-36,
Organism=Caenorhabditis elegans, GI71996771, Length=249, Percent_Identity=26.5060240963855, Blast_Score=86, Evalue=3e-17,
Organism=Drosophila melanogaster, GI21356207, Length=449, Percent_Identity=25.8351893095768, Blast_Score=150, Evalue=2e-36,
Organism=Drosophila melanogaster, GI19922432, Length=390, Percent_Identity=24.3589743589744, Blast_Score=103, Evalue=2e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RIMO_HERA2 (A9AZS3)

Other databases:

- EMBL:   CP000875
- RefSeq:   YP_001547255.1
- ProteinModelPortal:   A9AZS3
- SMR:   A9AZS3
- GeneID:   5736346
- GenomeReviews:   CP000875_GR
- KEGG:   hau:Haur_4495
- HOGENOM:   HBG457663
- OMA:   KADAPEI
- ProtClustDB:   CLSK973014
- BioCyc:   HAUR316274:HAUR_4495-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01865
- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR000385
- InterPro:   IPR012340
- InterPro:   IPR007197
- InterPro:   IPR005840
- InterPro:   IPR002792
- Gene3D:   G3DSA:2.40.50.140
- PANTHER:   PTHR11918
- SMART:   SM00729
- TIGRFAMs:   TIGR01125
- TIGRFAMs:   TIGR00089

Pfam domain/function: PF04055 Radical_SAM; PF00919 UPF0004

EC number: NA

Molecular weight: Translated: 50948; Mature: 50948

Theoretical pI: Translated: 6.33; Mature: 6.33

Prosite motif: PS51449 MTTASE_N; PS01278 MTTASE_RADICAL; PS50926 TRAM; PS01305 MOAA_NIFB_PQQE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFHIITLGCPKNTVDSEGMHGILTREGHTAVDSSDGADVVIVNTCSFINAAREETVGVL
CEEEEEEECCCCCCCCCCCCCEEEECCCCCCCCCCCCCCEEEEECHHHHHHHHHHHHHHH
QELANNKAPGQKLIAAGCMAESHGDVLRSRVPKLDATLSTKEWMRIGSVVAGGVAPSKTT
HHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCCCC
GFGIPLMGGAPSAMPSTGLNLSLTPAATGDSLGAYGDWRTTAITRNKRGPSAYLKISDGC
CCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCEEEEEECCCCCCCEEEEECCCC
NLRCAFCTIPSFKGDMRSKAVGSILGEARELVEAGVQEIILVAQHLTDYGRDLGMKQNGL
CEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCH
GVLLEELAAVVPADRWIRLMYAYPQSVTPDLVETMARLPQLCHYVDMPLQHAHPDTLRRM
HHHHHHHHHHCCCHHHEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCHHCCCHHHHHHH
RRPPDTDKTKAIVNSLRQAMPDLSLRTTFIVGYPGETRDEFKALLEFLEEMQFDRVGMFR
CCCCCCHHHHHHHHHHHHHCCCCEEEEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCEEE
YSLEPGTVAGELPDQVAERVKERRWNEAMAVQQVISRARTARFVGQTMKVLVEGTGTDDD
EECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCC
GRAIVVGRSYRDAPEVDGLVFGYGAADVGQFAKIAINKTTDYDLWGEIV
CCEEEEECCCCCCCCCCCEEEECCCHHHHHHHEEEEECCCCCCCCCCCC
>Mature Secondary Structure
MKFHIITLGCPKNTVDSEGMHGILTREGHTAVDSSDGADVVIVNTCSFINAAREETVGVL
CEEEEEEECCCCCCCCCCCCCEEEECCCCCCCCCCCCCCEEEEECHHHHHHHHHHHHHHH
QELANNKAPGQKLIAAGCMAESHGDVLRSRVPKLDATLSTKEWMRIGSVVAGGVAPSKTT
HHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCCCC
GFGIPLMGGAPSAMPSTGLNLSLTPAATGDSLGAYGDWRTTAITRNKRGPSAYLKISDGC
CCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCEEEEEECCCCCCCEEEEECCCC
NLRCAFCTIPSFKGDMRSKAVGSILGEARELVEAGVQEIILVAQHLTDYGRDLGMKQNGL
CEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCH
GVLLEELAAVVPADRWIRLMYAYPQSVTPDLVETMARLPQLCHYVDMPLQHAHPDTLRRM
HHHHHHHHHHCCCHHHEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCHHCCCHHHHHHH
RRPPDTDKTKAIVNSLRQAMPDLSLRTTFIVGYPGETRDEFKALLEFLEEMQFDRVGMFR
CCCCCCHHHHHHHHHHHHHCCCCEEEEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCEEE
YSLEPGTVAGELPDQVAERVKERRWNEAMAVQQVISRARTARFVGQTMKVLVEGTGTDDD
EECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCC
GRAIVVGRSYRDAPEVDGLVFGYGAADVGQFAKIAINKTTDYDLWGEIV
CCEEEEECCCCCCCCCCCEEEECCCHHHHHHHEEEEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA