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The map label for this gene is ispB [C]

Identifier: 159901006

GI number: 159901006

Start: 5754252

End: 5755271

Strand: Reverse

Name: ispB [C]

Synonym: Haur_4493

Alternate gene names: 159901006

Gene position: 5755271-5754252 (Counterclockwise)

Preceding gene: 159901008

Following gene: 159901005

Centisome position: 90.68

GC content: 51.96

Gene sequence:

>1020_bases
ATGAAACACATTTCTGACGAATCGATTCGCCAAGCAATGCAGAGTGCCTTCCCACCTGCCGATGCTCGTGTAACCATGTT
TTATGAAATGCAGGAATATCATTTGGGCTGGCGTAATGCTCAGCTTGAGCCAACCCAAGCCGATAGTGGCAAGTTACTGC
GTCCGCGCTTTTGTTTGCTCGCCTGCGCTGCGGTTGGCGGCGATCCCCAACAGGCTGAGCCTTTAGCCGCCGCAATTCAG
CTGCTCCATGATTTTTCGCTTATCCACGATGATATTGAAGATCACAGCCCAACTCGCCGTGGCCGCGAAACCGTGTGGAA
ATTGTGGGAAGTTCCACAAGCGATCAATGTTGGCGATGGTATGTTTACGCTTGCCCAGCTTTCATTATTTCGTTTGGCAG
AAGTTGGGGTCGAATCATCGGTCGTGGTCGAAATTGCACGGCGCTTTAATCAAACCATTATTCGTTTATGCGAAGGCCAG
TATCTCGATATGTCGTTTGAGCAGCGCCTCGACATCAGCGAAGGCGATTATTTGGCGATGATCAGCCGCAAAACGGCAGC
ATTAATTGCCGCTGCTGCTGGTTTGGGGGCAATTTTGGGCAATGCCAACCGCGAACAAGCCGCCGCCCTGTATAATTGGG
GTGAAGCCTTGGGCTTGGCCTTCCAAATTGAAGATGATATGCTCGGCATTTGGGGCGCAGAAGCCGTCACTGGCAAGCCC
GATGCTCACGATATTTGGGGCCGCAAAAAAAGCCTGCCAATTATCCATGCCCTAGCTCACGCCGATGCTGAGGATGGCGG
CAAGCTGGCGGCGATTTATCAAAAAGAGCCGCTTGAGGCCAGCGATATTCAAATTGTGCTGACAATTTTAGAGCGCACTG
GCTCACAAGGCTATACTGCGGGCGTGGCCAAGTTCTATCACGAGCAAGCCTTAGCCGCCCTAGCCGATTTACAAGGCGAG
GCCGAGCCAATTGCCGAGTTGCATGCATTAACCAAACAATTACTGGGACGAGTGAAATAG

Upstream 100 bases:

>100_bases
TGGTGCAACGGGTTGTTCTTAGCTGTCTTGAGTGTACGCCCTTGCAAGAAATGGTACTAGAGCCGAAGATTACAAAGACA
TTACGTAGTACGAGGTTACT

Downstream 100 bases:

>100_bases
CTGGGCAATCAGCGCTGCGGGGTCTAGCCCAGCAATCTGCTAACCAAGGAGTAAAGGTTGGGCTGCGGCCTTGCTGCTAG
CCACCGAGAATACATGATGC

Product: polyprenyl synthetase

Products: NA

Alternate protein names: GGPP synthase; GGPPSase; (2E,6E)-farnesyl diphosphate synthase; Dimethylallyltranstransferase; Farnesyl diphosphate synthase; Farnesyltranstransferase; Geranylgeranyl diphosphate synthase; Geranyltranstransferase [H]

Number of amino acids: Translated: 339; Mature: 339

Protein sequence:

>339_residues
MKHISDESIRQAMQSAFPPADARVTMFYEMQEYHLGWRNAQLEPTQADSGKLLRPRFCLLACAAVGGDPQQAEPLAAAIQ
LLHDFSLIHDDIEDHSPTRRGRETVWKLWEVPQAINVGDGMFTLAQLSLFRLAEVGVESSVVVEIARRFNQTIIRLCEGQ
YLDMSFEQRLDISEGDYLAMISRKTAALIAAAAGLGAILGNANREQAAALYNWGEALGLAFQIEDDMLGIWGAEAVTGKP
DAHDIWGRKKSLPIIHALAHADAEDGGKLAAIYQKEPLEASDIQIVLTILERTGSQGYTAGVAKFYHEQALAALADLQGE
AEPIAELHALTKQLLGRVK

Sequences:

>Translated_339_residues
MKHISDESIRQAMQSAFPPADARVTMFYEMQEYHLGWRNAQLEPTQADSGKLLRPRFCLLACAAVGGDPQQAEPLAAAIQ
LLHDFSLIHDDIEDHSPTRRGRETVWKLWEVPQAINVGDGMFTLAQLSLFRLAEVGVESSVVVEIARRFNQTIIRLCEGQ
YLDMSFEQRLDISEGDYLAMISRKTAALIAAAAGLGAILGNANREQAAALYNWGEALGLAFQIEDDMLGIWGAEAVTGKP
DAHDIWGRKKSLPIIHALAHADAEDGGKLAAIYQKEPLEASDIQIVLTILERTGSQGYTAGVAKFYHEQALAALADLQGE
AEPIAELHALTKQLLGRVK
>Mature_339_residues
MKHISDESIRQAMQSAFPPADARVTMFYEMQEYHLGWRNAQLEPTQADSGKLLRPRFCLLACAAVGGDPQQAEPLAAAIQ
LLHDFSLIHDDIEDHSPTRRGRETVWKLWEVPQAINVGDGMFTLAQLSLFRLAEVGVESSVVVEIARRFNQTIIRLCEGQ
YLDMSFEQRLDISEGDYLAMISRKTAALIAAAAGLGAILGNANREQAAALYNWGEALGLAFQIEDDMLGIWGAEAVTGKP
DAHDIWGRKKSLPIIHALAHADAEDGGKLAAIYQKEPLEASDIQIVLTILERTGSQGYTAGVAKFYHEQALAALADLQGE
AEPIAELHALTKQLLGRVK

Specific function: Catalyzes the trans-addition of the three molecules of IPP onto DMAPP to form geranylgeranyl pyrophosphate which is a precursor of the ether-linked lipids [H]

COG id: COG0142

COG function: function code H; Geranylgeranyl pyrophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FPP/GGPP synthase family [H]

Homologues:

Organism=Homo sapiens, GI50659086, Length=224, Percent_Identity=28.125, Blast_Score=89, Evalue=4e-18,
Organism=Homo sapiens, GI4758430, Length=257, Percent_Identity=26.8482490272374, Blast_Score=80, Evalue=3e-15,
Organism=Homo sapiens, GI83700220, Length=257, Percent_Identity=26.8482490272374, Blast_Score=80, Evalue=3e-15,
Organism=Escherichia coli, GI1789578, Length=266, Percent_Identity=31.203007518797, Blast_Score=104, Evalue=1e-23,
Organism=Escherichia coli, GI1786623, Length=220, Percent_Identity=34.5454545454545, Blast_Score=74, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17505681, Length=239, Percent_Identity=28.0334728033473, Blast_Score=74, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6325188, Length=293, Percent_Identity=26.962457337884, Blast_Score=86, Evalue=8e-18,
Organism=Drosophila melanogaster, GI24651612, Length=270, Percent_Identity=25.9259259259259, Blast_Score=95, Evalue=5e-20,
Organism=Drosophila melanogaster, GI281365769, Length=243, Percent_Identity=30.4526748971193, Blast_Score=81, Evalue=9e-16,
Organism=Drosophila melanogaster, GI24660002, Length=243, Percent_Identity=30.4526748971193, Blast_Score=81, Evalue=9e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000092
- InterPro:   IPR017446
- InterPro:   IPR008949 [H]

Pfam domain/function: PF00348 polyprenyl_synt [H]

EC number: =2.5.1.1; =2.5.1.29; =2.5.1.10 [H]

Molecular weight: Translated: 37153; Mature: 37153

Theoretical pI: Translated: 4.83; Mature: 4.83

Prosite motif: PS00012 PHOSPHOPANTETHEINE ; PS00723 POLYPRENYL_SYNTHET_1 ; PS00444 POLYPRENYL_SYNTHET_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKHISDESIRQAMQSAFPPADARVTMFYEMQEYHLGWRNAQLEPTQADSGKLLRPRFCLL
CCCCCHHHHHHHHHHCCCCCCCEEEEEEEHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHH
ACAAVGGDPQQAEPLAAAIQLLHDFSLIHDDIEDHSPTRRGRETVWKLWEVPQAINVGDG
HHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCH
MFTLAQLSLFRLAEVGVESSVVVEIARRFNQTIIRLCEGQYLDMSFEQRLDISEGDYLAM
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCCCCCCCEEEE
ISRKTAALIAAAAGLGAILGNANREQAAALYNWGEALGLAFQIEDDMLGIWGAEAVTGKP
EHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHEEEEEECCCEEEECCCCCCCCCC
DAHDIWGRKKSLPIIHALAHADAEDGGKLAAIYQKEPLEASDIQIVLTILERTGSQGYTA
CCHHHCCCCCCCHHHHHHHHCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHCCCCCCHH
GVAKFYHEQALAALADLQGEAEPIAELHALTKQLLGRVK
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKHISDESIRQAMQSAFPPADARVTMFYEMQEYHLGWRNAQLEPTQADSGKLLRPRFCLL
CCCCCHHHHHHHHHHCCCCCCCEEEEEEEHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHH
ACAAVGGDPQQAEPLAAAIQLLHDFSLIHDDIEDHSPTRRGRETVWKLWEVPQAINVGDG
HHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCH
MFTLAQLSLFRLAEVGVESSVVVEIARRFNQTIIRLCEGQYLDMSFEQRLDISEGDYLAM
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCCCCCCCEEEE
ISRKTAALIAAAAGLGAILGNANREQAAALYNWGEALGLAFQIEDDMLGIWGAEAVTGKP
EHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHEEEEEECCCEEEECCCCCCCCCC
DAHDIWGRKKSLPIIHALAHADAEDGGKLAAIYQKEPLEASDIQIVLTILERTGSQGYTA
CCHHHCCCCCCCHHHHHHHHCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHCCCCCCHH
GVAKFYHEQALAALADLQGEAEPIAELHALTKQLLGRVK
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]