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The map label for this gene is liaS [H]

Identifier: 159900999

GI number: 159900999

Start: 5743634

End: 5745382

Strand: Reverse

Name: liaS [H]

Synonym: Haur_4486

Alternate gene names: 159900999

Gene position: 5745382-5743634 (Counterclockwise)

Preceding gene: 159901003

Following gene: 159900998

Centisome position: 90.53

GC content: 53.23

Gene sequence:

>1749_bases
ATGGATATACCAGATCCCGCTCGGGCTTCCGCAAGCCTACCAGCGGCAATCAATTCGATCTCCACCGATTGGCGAGCCTG
TCTAAGCACGCTCAACCGAATTGCTCACCTCATGGCGCAACATCTTGACCTCACAGCATTATTGCAGCATGCCCTCGTGA
TTATTAGCGAGGACTTTAAGTTTTCGACTCTGCGCTTGTTGTTGGTCAATCGGCCTGAGGCTGATGGCTTGATCTGTCGG
GCAGGAATTGGCCCAGCTACGCTTCCGTTGGAGCTTGATACGCTTTTTCCGCCTGACCAAGGCATTATTGGGGCAGCGGT
GCAGCTCCGTCAGCTGATTTGGATCGATAATGCACCAGGTTTATCGCATTTGGCGATTCCGATCATTATTAGCGAGCAGC
TTGAAGGAATTCTCTACATCGAATCGCAGCGCCAGGTGCTGGCTGATGATTCCACCAATTTGGCGATTGTGGCTGATCAG
CTGGGCGTGGCGATTGAAAATGCTTGTCTGTTTGATCAGCTTCAGCACCATTTGGCCGAAACCAAATTGATGTTTGAGAC
CAGCCACAACATTAGTAGTGCTCGCACAGTCGCCGAGGTGGTGGCGGCCTACCTCAATCATATTGCTGCCCGCCGGCGTT
ATGCCTGCACGGTTGTGTTGTATGAATTTGATGCCCAAGGCCAGTTTGTGGCTCGCACGGTCAAAGGGCGTTGGTCGCCA
GAACATGGCTTATCCTCGCACCACGATCGCCAGCCCCATGAACGTGATGCCTTAGACGATTTGCTTGATGCTGGCGAGAC
CGTAACAATTGCCGATGTGCGTACTGATCCACGGGTTGCGCCTAGCTTGCGGCGTATGCAAGAAGCTGAACAGCGGCTGG
CGATGGCCTTTATCCCGCTAATTGTGCGCAACCAGCGGATTGGCTTGGTTGTGTTGACTGCTGCCAAGCCGCACGCGTGG
TCACAGGCCGATCTCCATCCTTATCAAGTTACCGCCGAACATCTAGCGATCGTCATTGACAATCGTCGCCAACAACGGCT
GCTGTATGCCCACGGCGAACGGATTGCGGTGTTGGATGAGCGCCAGCGACTCGCCCGCGATCTGCATGATTCGGTAACCC
AAATGATCTTCAGCATTACGTTGATCTCGCAAACCGTTACCTCGGCATGGCAGCGTGATCCGGCTGAAGGCGAGCGCCGC
GTTAATCGCTTGATCGAATTGAGCCAACTAGCGCTGGCCGAAATGCGTTCATTATTGACCGATCTGCATCCGATTGAGCG
TGAGCATCCGCAGGCTGTGCTTTATGCTCAACCAACACCATCTTCAGCCCCCGATCAACCAAGTAAATTACTGCAACGAT
TGCATAAATATGCCACCGAGGTGATTGGCGAGCAGCTTCAGGTTAGCTTTGATACGACCAGTTACGAGCAACAGTCAGCT
GTCTGTGAAGATGCACTTTATCGAATTATCCAAGAAGCACTAAATAATGTCAGCAAACATGCCCAGGCCAGTCATGTGGC
GGTAACCCTTCGCGTTCATGGAAATCGGTTGATCTTGAGCGTTCAGGACGATGGGATTGGCTTCGATCAATCGAGCCAGT
CAGTGAGCCAAGGCTTAGGTCTCTACAGCATGCGTAAACGGGTTGAAGCCTGTGGCGGAACACTGACGATGACTCTCGGA
GCAACCGCAGGAACGGTGGTGCAAGCGATCATCCCGCGCGAAGCACCTCATGTTAGGAGCGAGCCATGA

Upstream 100 bases:

>100_bases
GGCAACCACCAATCAAACGTCCCTCGCCCACCGTCGTGGGTGAGAGAGTAACGTTTGTTGATGAAATACACCATTTCCAC
TAGCTTAAGGATGTAGGTGA

Downstream 100 bases:

>100_bases
CCAACATTATCCGAATTATGCTTGTTGACGACCATGCCATTGTGCGTGAAGGGCTGCGTACCCTCTTAGCCGAAGATACC
GAATTTTTGATTGTCGGCGA

Product: GAF sensor signal transduction histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 582; Mature: 582

Protein sequence:

>582_residues
MDIPDPARASASLPAAINSISTDWRACLSTLNRIAHLMAQHLDLTALLQHALVIISEDFKFSTLRLLLVNRPEADGLICR
AGIGPATLPLELDTLFPPDQGIIGAAVQLRQLIWIDNAPGLSHLAIPIIISEQLEGILYIESQRQVLADDSTNLAIVADQ
LGVAIENACLFDQLQHHLAETKLMFETSHNISSARTVAEVVAAYLNHIAARRRYACTVVLYEFDAQGQFVARTVKGRWSP
EHGLSSHHDRQPHERDALDDLLDAGETVTIADVRTDPRVAPSLRRMQEAEQRLAMAFIPLIVRNQRIGLVVLTAAKPHAW
SQADLHPYQVTAEHLAIVIDNRRQQRLLYAHGERIAVLDERQRLARDLHDSVTQMIFSITLISQTVTSAWQRDPAEGERR
VNRLIELSQLALAEMRSLLTDLHPIEREHPQAVLYAQPTPSSAPDQPSKLLQRLHKYATEVIGEQLQVSFDTTSYEQQSA
VCEDALYRIIQEALNNVSKHAQASHVAVTLRVHGNRLILSVQDDGIGFDQSSQSVSQGLGLYSMRKRVEACGGTLTMTLG
ATAGTVVQAIIPREAPHVRSEP

Sequences:

>Translated_582_residues
MDIPDPARASASLPAAINSISTDWRACLSTLNRIAHLMAQHLDLTALLQHALVIISEDFKFSTLRLLLVNRPEADGLICR
AGIGPATLPLELDTLFPPDQGIIGAAVQLRQLIWIDNAPGLSHLAIPIIISEQLEGILYIESQRQVLADDSTNLAIVADQ
LGVAIENACLFDQLQHHLAETKLMFETSHNISSARTVAEVVAAYLNHIAARRRYACTVVLYEFDAQGQFVARTVKGRWSP
EHGLSSHHDRQPHERDALDDLLDAGETVTIADVRTDPRVAPSLRRMQEAEQRLAMAFIPLIVRNQRIGLVVLTAAKPHAW
SQADLHPYQVTAEHLAIVIDNRRQQRLLYAHGERIAVLDERQRLARDLHDSVTQMIFSITLISQTVTSAWQRDPAEGERR
VNRLIELSQLALAEMRSLLTDLHPIEREHPQAVLYAQPTPSSAPDQPSKLLQRLHKYATEVIGEQLQVSFDTTSYEQQSA
VCEDALYRIIQEALNNVSKHAQASHVAVTLRVHGNRLILSVQDDGIGFDQSSQSVSQGLGLYSMRKRVEACGGTLTMTLG
ATAGTVVQAIIPREAPHVRSEP
>Mature_582_residues
MDIPDPARASASLPAAINSISTDWRACLSTLNRIAHLMAQHLDLTALLQHALVIISEDFKFSTLRLLLVNRPEADGLICR
AGIGPATLPLELDTLFPPDQGIIGAAVQLRQLIWIDNAPGLSHLAIPIIISEQLEGILYIESQRQVLADDSTNLAIVADQ
LGVAIENACLFDQLQHHLAETKLMFETSHNISSARTVAEVVAAYLNHIAARRRYACTVVLYEFDAQGQFVARTVKGRWSP
EHGLSSHHDRQPHERDALDDLLDAGETVTIADVRTDPRVAPSLRRMQEAEQRLAMAFIPLIVRNQRIGLVVLTAAKPHAW
SQADLHPYQVTAEHLAIVIDNRRQQRLLYAHGERIAVLDERQRLARDLHDSVTQMIFSITLISQTVTSAWQRDPAEGERR
VNRLIELSQLALAEMRSLLTDLHPIEREHPQAVLYAQPTPSSAPDQPSKLLQRLHKYATEVIGEQLQVSFDTTSYEQQSA
VCEDALYRIIQEALNNVSKHAQASHVAVTLRVHGNRLILSVQDDGIGFDQSSQSVSQGLGLYSMRKRVEACGGTLTMTLG
ATAGTVVQAIIPREAPHVRSEP

Specific function: Member of the two-component regulatory system liaS/liaR probably involved in response to a subset of cell wall-active antibiotics that interfere with the lipid II cycle in the cytoplasmic membrane (bacitracin, nisin, ramoplanin and vancomycin). Seems also

COG id: COG4585

COG function: function code T; Signal transduction histidine kinase

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI87082311, Length=99, Percent_Identity=41.4141414141414, Blast_Score=66, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR011712
- InterPro:   IPR005467
- InterPro:   IPR017202 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF07730 HisKA_3 [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 64446; Mature: 64446

Theoretical pI: Translated: 6.30; Mature: 6.30

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDIPDPARASASLPAAINSISTDWRACLSTLNRIAHLMAQHLDLTALLQHALVIISEDFK
CCCCCCCCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHEEEECCCC
FSTLRLLLVNRPEADGLICRAGIGPATLPLELDTLFPPDQGIIGAAVQLRQLIWIDNAPG
EEEEEEEEEECCCCCCEEEECCCCCCCCCEEECCCCCCCCCHHHHHHHHHHHHEECCCCC
LSHLAIPIIISEQLEGILYIESQRQVLADDSTNLAIVADQLGVAIENACLFDQLQHHLAE
CCCEEEEEEEECCCCEEEEEECCCCEECCCCCCEEEEEHHHCHHHHHHHHHHHHHHHHHH
TKLMFETSHNISSARTVAEVVAAYLNHIAARRRYACTVVLYEFDAQGQFVARTVKGRWSP
HHEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCEEEEEEEEEECCCCCEEEEECCCCCCC
EHGLSSHHDRQPHERDALDDLLDAGETVTIADVRTDPRVAPSLRRMQEAEQRLAMAFIPL
CCCCCCCCCCCCCHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHH
IVRNQRIGLVVLTAAKPHAWSQADLHPYQVTAEHLAIVIDNRRQQRLLYAHGERIAVLDE
HHCCCCCCEEEEEECCCCCCCCCCCCCCEEEHHHEEEEECCCCHHHHHHCCCCEEEEHHH
RQRLARDLHDSVTQMIFSITLISQTVTSAWQRDPAEGERRVNRLIELSQLALAEMRSLLT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
DLHPIEREHPQAVLYAQPTPSSAPDQPSKLLQRLHKYATEVIGEQLQVSFDTTSYEQQSA
HCCCCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHCCHHEEEEECCCCCHHHHH
VCEDALYRIIQEALNNVSKHAQASHVAVTLRVHGNRLILSVQDDGIGFDQSSQSVSQGLG
HHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCEEEEEEECCCCCCCCCHHHHHHCCC
LYSMRKRVEACGGTLTMTLGATAGTVVQAIIPREAPHVRSEP
HHHHHHHHHHCCCEEEEEECCCHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MDIPDPARASASLPAAINSISTDWRACLSTLNRIAHLMAQHLDLTALLQHALVIISEDFK
CCCCCCCCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHEEEECCCC
FSTLRLLLVNRPEADGLICRAGIGPATLPLELDTLFPPDQGIIGAAVQLRQLIWIDNAPG
EEEEEEEEEECCCCCCEEEECCCCCCCCCEEECCCCCCCCCHHHHHHHHHHHHEECCCCC
LSHLAIPIIISEQLEGILYIESQRQVLADDSTNLAIVADQLGVAIENACLFDQLQHHLAE
CCCEEEEEEEECCCCEEEEEECCCCEECCCCCCEEEEEHHHCHHHHHHHHHHHHHHHHHH
TKLMFETSHNISSARTVAEVVAAYLNHIAARRRYACTVVLYEFDAQGQFVARTVKGRWSP
HHEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCEEEEEEEEEECCCCCEEEEECCCCCCC
EHGLSSHHDRQPHERDALDDLLDAGETVTIADVRTDPRVAPSLRRMQEAEQRLAMAFIPL
CCCCCCCCCCCCCHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHH
IVRNQRIGLVVLTAAKPHAWSQADLHPYQVTAEHLAIVIDNRRQQRLLYAHGERIAVLDE
HHCCCCCCEEEEEECCCCCCCCCCCCCCEEEHHHEEEEECCCCHHHHHHCCCCEEEEHHH
RQRLARDLHDSVTQMIFSITLISQTVTSAWQRDPAEGERRVNRLIELSQLALAEMRSLLT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
DLHPIEREHPQAVLYAQPTPSSAPDQPSKLLQRLHKYATEVIGEQLQVSFDTTSYEQQSA
HCCCCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHCCHHEEEEECCCCCHHHHH
VCEDALYRIIQEALNNVSKHAQASHVAVTLRVHGNRLILSVQDDGIGFDQSSQSVSQGLG
HHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCEEEEEEECCCCCCCCCHHHHHHCCC
LYSMRKRVEACGGTLTMTLGATAGTVVQAIIPREAPHVRSEP
HHHHHHHHHHCCCEEEEEECCCHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9639930; 9384377 [H]