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The map label for this gene is yfiK [H]

Identifier: 159900998

GI number: 159900998

Start: 5742996

End: 5743637

Strand: Reverse

Name: yfiK [H]

Synonym: Haur_4485

Alternate gene names: 159900998

Gene position: 5743637-5742996 (Counterclockwise)

Preceding gene: 159900999

Following gene: 159900997

Centisome position: 90.5

GC content: 51.71

Gene sequence:

>642_bases
ATGACCAACATTATCCGAATTATGCTTGTTGACGACCATGCCATTGTGCGTGAAGGGCTGCGTACCCTCTTAGCCGAAGA
TACCGAATTTTTGATTGTCGGCGAGGCTCAAAATGGAGCCGACGCGATCGAATTGGCAGCAGTTGTGCGGCCACATGTTA
TTTTGATGGATCTTGTGATGCCCAATGTCGATGGCATTCAGGCCACTCAAGCGCTTCAAAGTCTCATGCCCAGCAGCCGA
ATTATCGTCCTGACCAGTTTTAGTGATGATCAATGGGTCAGCAAGGCAATTCAGGCGGGTGCTAGTGGCTACCTGCTGAA
AAATGTGCTCAAGGCCGAGTTGTTTCTGGCAATTCGCACAGTTGCGAACGGCCAGCCATTTTTACATGCCGAAGCCCAGC
GCACCTTGATGCGTCAATCAACCGCTCCGCCTTCGCTGTTGCCAAGCCTAACTGAGCGTGAGCGCAGTGTGCTCGATGGG
ATTGTCGGCGGTTTGAGCAATAAAGAAATTGCTCGTTCGCTGCATCTGACCGAGGGCACAGTTAAAGGCTATGTGAGCAC
CATACTCACCAAGTTGGCGGTGGCCGATCGAACTCAAGCGGCGCTGTATGCCATCAAACATGGCTTTGATCTGACCCAGT
AA

Upstream 100 bases:

>100_bases
AAGCCTGTGGCGGAACACTGACGATGACTCTCGGAGCAACCGCAGGAACGGTGGTGCAAGCGATCATCCCGCGCGAAGCA
CCTCATGTTAGGAGCGAGCC

Downstream 100 bases:

>100_bases
TGTGTCAATCCTATCTTTCGTTAGGGGTCCCAACTACCTTTTGATAGCCCCAATTCCTAGCCTAGGCTAGGTGTGGCTTG
CCAAGTATTCTCGTATCCTA

Product: two component LuxR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 213; Mature: 212

Protein sequence:

>213_residues
MTNIIRIMLVDDHAIVREGLRTLLAEDTEFLIVGEAQNGADAIELAAVVRPHVILMDLVMPNVDGIQATQALQSLMPSSR
IIVLTSFSDDQWVSKAIQAGASGYLLKNVLKAELFLAIRTVANGQPFLHAEAQRTLMRQSTAPPSLLPSLTERERSVLDG
IVGGLSNKEIARSLHLTEGTVKGYVSTILTKLAVADRTQAALYAIKHGFDLTQ

Sequences:

>Translated_213_residues
MTNIIRIMLVDDHAIVREGLRTLLAEDTEFLIVGEAQNGADAIELAAVVRPHVILMDLVMPNVDGIQATQALQSLMPSSR
IIVLTSFSDDQWVSKAIQAGASGYLLKNVLKAELFLAIRTVANGQPFLHAEAQRTLMRQSTAPPSLLPSLTERERSVLDG
IVGGLSNKEIARSLHLTEGTVKGYVSTILTKLAVADRTQAALYAIKHGFDLTQ
>Mature_212_residues
TNIIRIMLVDDHAIVREGLRTLLAEDTEFLIVGEAQNGADAIELAAVVRPHVILMDLVMPNVDGIQATQALQSLMPSSRI
IVLTSFSDDQWVSKAIQAGASGYLLKNVLKAELFLAIRTVANGQPFLHAEAQRTLMRQSTAPPSLLPSLTERERSVLDGI
VGGLSNKEIARSLHLTEGTVKGYVSTILTKLAVADRTQAALYAIKHGFDLTQ

Specific function: Member of the two-component regulatory system yfiJ/yfiK [H]

COG id: COG2197

COG function: function code TK; Response regulator containing a CheY-like receiver domain and an HTH DNA-binding domain

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1788222, Length=209, Percent_Identity=33.9712918660287, Blast_Score=118, Evalue=3e-28,
Organism=Escherichia coli, GI1788521, Length=205, Percent_Identity=37.0731707317073, Blast_Score=109, Evalue=1e-25,
Organism=Escherichia coli, GI1787473, Length=205, Percent_Identity=36.0975609756098, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1786747, Length=199, Percent_Identity=28.1407035175879, Blast_Score=92, Evalue=3e-20,
Organism=Escherichia coli, GI1790102, Length=199, Percent_Identity=31.1557788944724, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI1788712, Length=185, Percent_Identity=27.027027027027, Blast_Score=82, Evalue=2e-17,
Organism=Escherichia coli, GI87082052, Length=113, Percent_Identity=35.3982300884956, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1788550, Length=119, Percent_Identity=34.453781512605, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI1789937, Length=127, Percent_Identity=27.5590551181102, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001789
- InterPro:   IPR000792
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00196 GerE; PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 23095; Mature: 22964

Theoretical pI: Translated: 6.36; Mature: 6.36

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS50043 HTH_LUXR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNIIRIMLVDDHAIVREGLRTLLAEDTEFLIVGEAQNGADAIELAAVVRPHVILMDLVM
CCCEEEEEEECCHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHC
PNVDGIQATQALQSLMPSSRIIVLTSFSDDQWVSKAIQAGASGYLLKNVLKAELFLAIRT
CCCCCHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
VANGQPFLHAEAQRTLMRQSTAPPSLLPSLTERERSVLDGIVGGLSNKEIARSLHLTEGT
HCCCCCEEHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCHHH
VKGYVSTILTKLAVADRTQAALYAIKHGFDLTQ
HHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
TNIIRIMLVDDHAIVREGLRTLLAEDTEFLIVGEAQNGADAIELAAVVRPHVILMDLVM
CCEEEEEEECCHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHC
PNVDGIQATQALQSLMPSSRIIVLTSFSDDQWVSKAIQAGASGYLLKNVLKAELFLAIRT
CCCCCHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
VANGQPFLHAEAQRTLMRQSTAPPSLLPSLTERERSVLDGIVGGLSNKEIARSLHLTEGT
HCCCCCEEHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCHHH
VKGYVSTILTKLAVADRTQAALYAIKHGFDLTQ
HHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8973323; 9384377 [H]