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The map label for this gene is rlmN

Identifier: 159900818

GI number: 159900818

Start: 5496695

End: 5497786

Strand: Reverse

Name: rlmN

Synonym: Haur_4305

Alternate gene names: 159900818

Gene position: 5497786-5496695 (Counterclockwise)

Preceding gene: 159900820

Following gene: 159900817

Centisome position: 86.63

GC content: 52.56

Gene sequence:

>1092_bases
ATGAATATTTACGATCTTGATTTGAAGGGCTTGACCGCGCAATTGACCGAATTAGGCCAACCAGCCTTTCGGGCGCGGCA
AATTTATGCCCATTTATATAAAAAATTAGCTAACGATTTTGCCGCCATGACCGATTTGCCCGCCGCACTGCGTGAGCAAT
TGACAGCGAATTTGCAGATTGGCAGCCTTGAATTGGTGCGCGAACAAACCACCGACGATGGCTTAACCCGCAAAGTGCTG
TGGCGCTGCCCAGGCGATGCGGTGATTGAAACTGTGCTGATGATCTACCCGCCAGATCGGGCGACGATTTGCGTTTCGAC
CCAAGCGGGTTGTGCCATGGGTTGCGTCTTTTGTGCAACTGGCAAATTAGGCTTGTTGCGCAATATCTCCAGCGGCGAGA
TTATGGAGCAGGTGTTGTATTTCGAGCGCTATCTGCGCAATGAAGGTGCTGCGATTGCCAAACGCCACGGCGGGCCAGTG
CCCGACCATATCACCAACTTGGTGTTTATGGGCATGGGCGAACCATTTGCCAACTACGAGCGTTGGTGGGCGGCGGTCGA
ACGGCTCAATGATAAACAGGGCTTTAATCTTGGCGCTCGCAACATGACCGTTTCAACCGTGGGCTTGGTCAAAGGTATTC
GCCAATTGGCCGATGAAGCCTTACAGGTCAATTTGGCGGTTTCGTTGCATGCTCCCAACGATCAATTGCGCAGCGAACTC
ATGCCCGTCAACGATCGCTTTGATATCAGCGATTTGATGGATGCGATTCGTTATTACACCGATAAAACCCATCGGCGGGT
TTCATTTGAATATGTGCTGCTCGATGATAAAAACGACACGCCTGAGCTTGCCGCCCAACTTGCCAGTTTGGTAAAGGGCA
TGCTCTGCCATGTCAATTTAATTCCGTGGAATCCTATCCCTGGAACGCCCTTGACGCGTTCGCATCGTGAGCGGGTTACG
GCTTTTCAACGAGTCGTGCAAGCAGCAGGCATTTCGTGCACCGTGCGAGTGGAGCGCGGGGTTGAAATCGCCGCCGCCTG
TGGCCAACTTGCCGCGATTCCATCGCCAACCAGCCCGATAAACCTGTATTAA

Upstream 100 bases:

>100_bases
AAAAACTTAACCTTCGTGCCCTTCGCGTCCTTCGTGGATCAACGACCGATCCCCACGATAAAGAAAGATGGTATCATGGG
GCGTAACAAACGAGGAACCC

Downstream 100 bases:

>100_bases
AGTCAGAATTCAGAAGGCAAAAATCAAAATAGTTAATTGATAAGCCGCTGTATTGCTGGATTCAGAACGATTGGAATTAA
CTGTATCAAGGCATGTAGGT

Product: ribosomal RNA large subunit methyltransferase N

Products: NA

Alternate protein names: 23S rRNA m2A2503 methyltransferase

Number of amino acids: Translated: 363; Mature: 363

Protein sequence:

>363_residues
MNIYDLDLKGLTAQLTELGQPAFRARQIYAHLYKKLANDFAAMTDLPAALREQLTANLQIGSLELVREQTTDDGLTRKVL
WRCPGDAVIETVLMIYPPDRATICVSTQAGCAMGCVFCATGKLGLLRNISSGEIMEQVLYFERYLRNEGAAIAKRHGGPV
PDHITNLVFMGMGEPFANYERWWAAVERLNDKQGFNLGARNMTVSTVGLVKGIRQLADEALQVNLAVSLHAPNDQLRSEL
MPVNDRFDISDLMDAIRYYTDKTHRRVSFEYVLLDDKNDTPELAAQLASLVKGMLCHVNLIPWNPIPGTPLTRSHRERVT
AFQRVVQAAGISCTVRVERGVEIAAACGQLAAIPSPTSPINLY

Sequences:

>Translated_363_residues
MNIYDLDLKGLTAQLTELGQPAFRARQIYAHLYKKLANDFAAMTDLPAALREQLTANLQIGSLELVREQTTDDGLTRKVL
WRCPGDAVIETVLMIYPPDRATICVSTQAGCAMGCVFCATGKLGLLRNISSGEIMEQVLYFERYLRNEGAAIAKRHGGPV
PDHITNLVFMGMGEPFANYERWWAAVERLNDKQGFNLGARNMTVSTVGLVKGIRQLADEALQVNLAVSLHAPNDQLRSEL
MPVNDRFDISDLMDAIRYYTDKTHRRVSFEYVLLDDKNDTPELAAQLASLVKGMLCHVNLIPWNPIPGTPLTRSHRERVT
AFQRVVQAAGISCTVRVERGVEIAAACGQLAAIPSPTSPINLY
>Mature_363_residues
MNIYDLDLKGLTAQLTELGQPAFRARQIYAHLYKKLANDFAAMTDLPAALREQLTANLQIGSLELVREQTTDDGLTRKVL
WRCPGDAVIETVLMIYPPDRATICVSTQAGCAMGCVFCATGKLGLLRNISSGEIMEQVLYFERYLRNEGAAIAKRHGGPV
PDHITNLVFMGMGEPFANYERWWAAVERLNDKQGFNLGARNMTVSTVGLVKGIRQLADEALQVNLAVSLHAPNDQLRSEL
MPVNDRFDISDLMDAIRYYTDKTHRRVSFEYVLLDDKNDTPELAAQLASLVKGMLCHVNLIPWNPIPGTPLTRSHRERVT
AFQRVVQAAGISCTVRVERGVEIAAACGQLAAIPSPTSPINLY

Specific function: Specifically methylates position 2 of adenine 2503 in 23S rRNA

COG id: COG0820

COG function: function code R; Predicted Fe-S-cluster redox enzyme

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. RlmN family

Homologues:

Organism=Escherichia coli, GI1788865, Length=355, Percent_Identity=41.4084507042254, Blast_Score=258, Evalue=5e-70,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLMN_HERA2 (A9AY55)

Other databases:

- EMBL:   CP000875
- RefSeq:   YP_001547065.1
- ProteinModelPortal:   A9AY55
- GeneID:   5736164
- GenomeReviews:   CP000875_GR
- KEGG:   hau:Haur_4305
- HOGENOM:   HBG335345
- OMA:   TFCSTAQ
- ProtClustDB:   PRK14461
- BioCyc:   HAUR316274:HAUR_4305-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01849
- InterPro:   IPR006638
- InterPro:   IPR007197
- InterPro:   IPR004383
- PIRSF:   PIRSF006004
- SMART:   SM00729
- TIGRFAMs:   TIGR00048

Pfam domain/function: PF04055 Radical_SAM

EC number: =2.1.1.192

Molecular weight: Translated: 40216; Mature: 40216

Theoretical pI: Translated: 6.85; Mature: 6.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIYDLDLKGLTAQLTELGQPAFRARQIYAHLYKKLANDFAAMTDLPAALREQLTANLQI
CCEEECCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCEE
GSLELVREQTTDDGLTRKVLWRCPGDAVIETVLMIYPPDRATICVSTQAGCAMGCVFCAT
CHHHHHHHHCCCCCHHHHHHHCCCCHHHHHHHHEEECCCCCEEEEECCCCHHHHHHHHHC
GKLGLLRNISSGEIMEQVLYFERYLRNEGAAIAKRHGGPVPDHITNLVFMGMGEPFANYE
CCHHHHHCCCCHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCHHHHHEEEECCCCCHHHHH
RWWAAVERLNDKQGFNLGARNMTVSTVGLVKGIRQLADEALQVNLAVSLHAPNDQLRSEL
HHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHEEEEEEEEECCCHHHHHHC
MPVNDRFDISDLMDAIRYYTDKTHRRVSFEYVLLDDKNDTPELAAQLASLVKGMLCHVNL
CCCCCCCCHHHHHHHHHHHHCCCCCEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHEEEE
IPWNPIPGTPLTRSHRERVTAFQRVVQAAGISCTVRVERGVEIAAACGQLAAIPSPTSPI
ECCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHCCCCCCCCC
NLY
CCC
>Mature Secondary Structure
MNIYDLDLKGLTAQLTELGQPAFRARQIYAHLYKKLANDFAAMTDLPAALREQLTANLQI
CCEEECCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCEE
GSLELVREQTTDDGLTRKVLWRCPGDAVIETVLMIYPPDRATICVSTQAGCAMGCVFCAT
CHHHHHHHHCCCCCHHHHHHHCCCCHHHHHHHHEEECCCCCEEEEECCCCHHHHHHHHHC
GKLGLLRNISSGEIMEQVLYFERYLRNEGAAIAKRHGGPVPDHITNLVFMGMGEPFANYE
CCHHHHHCCCCHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCHHHHHEEEECCCCCHHHHH
RWWAAVERLNDKQGFNLGARNMTVSTVGLVKGIRQLADEALQVNLAVSLHAPNDQLRSEL
HHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHEEEEEEEEECCCHHHHHHC
MPVNDRFDISDLMDAIRYYTDKTHRRVSFEYVLLDDKNDTPELAAQLASLVKGMLCHVNL
CCCCCCCCHHHHHHHHHHHHCCCCCEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHEEEE
IPWNPIPGTPLTRSHRERVTAFQRVVQAAGISCTVRVERGVEIAAACGQLAAIPSPTSPI
ECCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHCCCCCCCCC
NLY
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA